α-Galactosidases production by Debaryomyces hansenii UFV-1

• Autor(a) principal: Viana, Pollyanna Amaral
• Data de Publicação: 2011
• Outros Autores: Rezende, Sebastião Tavares de, Passos, Flávia Maria Lopes, Machado, Solimar Gonçalves, Maitan, Gabriela Picollo, Coelho, Vinicio Tadeu da Silva, Guimarães, Valéria Monteze
• Tipo de documento: Artigo
• Idioma: eng
• Título da fonte: LOCUS Repositório Institucional da UFV
• Texto Completo: http://dx.doi.org/10.1007/s10068-011-0085-7; http://www.locus.ufv.br/handle/123456789/21760
• topic: Debaryomyces hansenii UFV-1; α-galactosidase; Culture media; Carbon source; Enzyme production

Extracellular and intracellular α-galactosidases were produced by yeast Debaryomyces hansenii UFV-1 grown on different media with several carbon sources. D. hansenii grown in YP-medium (1% yeast extract and 2% peptone) presented maximum cell mass (8.45 mg/mL) after 36 h of cultivation, with lactose as carbon source, followed by sucrose, glucose, raffinose, and galactose. Higher extracellular and intracellular α-galactosidases activities were observed at 48 h of D. hansenii cultivation in YPmedium containing galactose (0.97 and 5.27 U/mL) and lactose (1.28 and 4.88 U/mL), supporting the evidence for the model of induction for the yeast GAL/MEL regulon, such as described in Sacharomyces cereviseae.