Extracellular α-Galactosidase from Debaryomyces hansenii UFV-1 and Its use in the hydrolysis of raffinose oligosaccharides
Autor(a) principal: | |
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Data de Publicação: | 2006 |
Outros Autores: | , , , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | LOCUS Repositório Institucional da UFV |
Texto Completo: | http://dx.doi.org/10.1021/jf0526442 http://www.locus.ufv.br/handle/123456789/19902 |
Resumo: | Raffinose oligosaccharides (RO) are the factors primarily responsible for flatulence upon ingestion of soybean-derived products. ROs are hydrolyzed by α-galactosidases that cleave α-1,6-linkages of α-galactoside residues. The objectives of this study were the purification and characterization of extracellular α-galactosidase from Debaryomyces hansenii UFV-1. The enzyme purified by gel filtration and anion exchange chromatographies presented an Mr value of 60 kDa and the N-terminal amino acid sequence YENGLNLVPQMGWN. The Km values for hydrolysis of pNPαGal, melibiose, stachyose, and raffinose were 0.30, 2.01, 9.66, and 16 mM, respectively. The α-galactosidase presented absolute specificity for galactose in the α-position, hydrolyzing pNPGal, stachyose, raffinose, melibiose, and polymers. The enzyme was noncompetitively inhibited by galactose (Ki = 2.7 mM) and melibiose (Ki = 1.2 mM). Enzyme treatments of soy milk for 4 h at 60 °C reduced the amounts of stachyose and raffinose by 100%. |
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LOCUS Repositório Institucional da UFV |
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Extracellular α-Galactosidase from Debaryomyces hansenii UFV-1 and Its use in the hydrolysis of raffinose oligosaccharidesα-GalactosidaseDebaryomyces hansenii UFV-1Raffinose oligosaccharidesCharacterizationFlatulenceRaffinose oligosaccharides (RO) are the factors primarily responsible for flatulence upon ingestion of soybean-derived products. ROs are hydrolyzed by α-galactosidases that cleave α-1,6-linkages of α-galactoside residues. The objectives of this study were the purification and characterization of extracellular α-galactosidase from Debaryomyces hansenii UFV-1. The enzyme purified by gel filtration and anion exchange chromatographies presented an Mr value of 60 kDa and the N-terminal amino acid sequence YENGLNLVPQMGWN. The Km values for hydrolysis of pNPαGal, melibiose, stachyose, and raffinose were 0.30, 2.01, 9.66, and 16 mM, respectively. The α-galactosidase presented absolute specificity for galactose in the α-position, hydrolyzing pNPGal, stachyose, raffinose, melibiose, and polymers. The enzyme was noncompetitively inhibited by galactose (Ki = 2.7 mM) and melibiose (Ki = 1.2 mM). Enzyme treatments of soy milk for 4 h at 60 °C reduced the amounts of stachyose and raffinose by 100%.Journal of Agricultural and Food Chemistry2018-06-04T12:00:06Z2018-06-04T12:00:06Z2006-02-17info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articlepdfapplication/pdf15205118http://dx.doi.org/10.1021/jf0526442http://www.locus.ufv.br/handle/123456789/19902engv. 54, n. 6, p. 2385–2391, Fevereiro 2006American Chemical Societyinfo:eu-repo/semantics/openAccessRezende, Sebastião T. deMarques, Virgínia M.Trevizano, Larissa M.Passos, Flávia M. L.Oliveira, Maria G. A.Bemquerer, Marcelo P.Oliveira, Jamil S.Guimarães, Valéria M.Viana, Pollyanna A.reponame:LOCUS Repositório Institucional da UFVinstname:Universidade Federal de Viçosa (UFV)instacron:UFV2024-07-12T06:41:47Zoai:locus.ufv.br:123456789/19902Repositório InstitucionalPUBhttps://www.locus.ufv.br/oai/requestfabiojreis@ufv.bropendoar:21452024-07-12T06:41:47LOCUS Repositório Institucional da UFV - Universidade Federal de Viçosa (UFV)false |
dc.title.none.fl_str_mv |
Extracellular α-Galactosidase from Debaryomyces hansenii UFV-1 and Its use in the hydrolysis of raffinose oligosaccharides |
title |
Extracellular α-Galactosidase from Debaryomyces hansenii UFV-1 and Its use in the hydrolysis of raffinose oligosaccharides |
spellingShingle |
Extracellular α-Galactosidase from Debaryomyces hansenii UFV-1 and Its use in the hydrolysis of raffinose oligosaccharides Rezende, Sebastião T. de α-Galactosidase Debaryomyces hansenii UFV-1 Raffinose oligosaccharides Characterization Flatulence |
title_short |
Extracellular α-Galactosidase from Debaryomyces hansenii UFV-1 and Its use in the hydrolysis of raffinose oligosaccharides |
title_full |
Extracellular α-Galactosidase from Debaryomyces hansenii UFV-1 and Its use in the hydrolysis of raffinose oligosaccharides |
title_fullStr |
Extracellular α-Galactosidase from Debaryomyces hansenii UFV-1 and Its use in the hydrolysis of raffinose oligosaccharides |
title_full_unstemmed |
Extracellular α-Galactosidase from Debaryomyces hansenii UFV-1 and Its use in the hydrolysis of raffinose oligosaccharides |
title_sort |
Extracellular α-Galactosidase from Debaryomyces hansenii UFV-1 and Its use in the hydrolysis of raffinose oligosaccharides |
author |
Rezende, Sebastião T. de |
author_facet |
Rezende, Sebastião T. de Marques, Virgínia M. Trevizano, Larissa M. Passos, Flávia M. L. Oliveira, Maria G. A. Bemquerer, Marcelo P. Oliveira, Jamil S. Guimarães, Valéria M. Viana, Pollyanna A. |
author_role |
author |
author2 |
Marques, Virgínia M. Trevizano, Larissa M. Passos, Flávia M. L. Oliveira, Maria G. A. Bemquerer, Marcelo P. Oliveira, Jamil S. Guimarães, Valéria M. Viana, Pollyanna A. |
author2_role |
author author author author author author author author |
dc.contributor.author.fl_str_mv |
Rezende, Sebastião T. de Marques, Virgínia M. Trevizano, Larissa M. Passos, Flávia M. L. Oliveira, Maria G. A. Bemquerer, Marcelo P. Oliveira, Jamil S. Guimarães, Valéria M. Viana, Pollyanna A. |
dc.subject.por.fl_str_mv |
α-Galactosidase Debaryomyces hansenii UFV-1 Raffinose oligosaccharides Characterization Flatulence |
topic |
α-Galactosidase Debaryomyces hansenii UFV-1 Raffinose oligosaccharides Characterization Flatulence |
description |
Raffinose oligosaccharides (RO) are the factors primarily responsible for flatulence upon ingestion of soybean-derived products. ROs are hydrolyzed by α-galactosidases that cleave α-1,6-linkages of α-galactoside residues. The objectives of this study were the purification and characterization of extracellular α-galactosidase from Debaryomyces hansenii UFV-1. The enzyme purified by gel filtration and anion exchange chromatographies presented an Mr value of 60 kDa and the N-terminal amino acid sequence YENGLNLVPQMGWN. The Km values for hydrolysis of pNPαGal, melibiose, stachyose, and raffinose were 0.30, 2.01, 9.66, and 16 mM, respectively. The α-galactosidase presented absolute specificity for galactose in the α-position, hydrolyzing pNPGal, stachyose, raffinose, melibiose, and polymers. The enzyme was noncompetitively inhibited by galactose (Ki = 2.7 mM) and melibiose (Ki = 1.2 mM). Enzyme treatments of soy milk for 4 h at 60 °C reduced the amounts of stachyose and raffinose by 100%. |
publishDate |
2006 |
dc.date.none.fl_str_mv |
2006-02-17 2018-06-04T12:00:06Z 2018-06-04T12:00:06Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
15205118 http://dx.doi.org/10.1021/jf0526442 http://www.locus.ufv.br/handle/123456789/19902 |
identifier_str_mv |
15205118 |
url |
http://dx.doi.org/10.1021/jf0526442 http://www.locus.ufv.br/handle/123456789/19902 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
v. 54, n. 6, p. 2385–2391, Fevereiro 2006 |
dc.rights.driver.fl_str_mv |
American Chemical Society info:eu-repo/semantics/openAccess |
rights_invalid_str_mv |
American Chemical Society |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
pdf application/pdf |
dc.publisher.none.fl_str_mv |
Journal of Agricultural and Food Chemistry |
publisher.none.fl_str_mv |
Journal of Agricultural and Food Chemistry |
dc.source.none.fl_str_mv |
reponame:LOCUS Repositório Institucional da UFV instname:Universidade Federal de Viçosa (UFV) instacron:UFV |
instname_str |
Universidade Federal de Viçosa (UFV) |
instacron_str |
UFV |
institution |
UFV |
reponame_str |
LOCUS Repositório Institucional da UFV |
collection |
LOCUS Repositório Institucional da UFV |
repository.name.fl_str_mv |
LOCUS Repositório Institucional da UFV - Universidade Federal de Viçosa (UFV) |
repository.mail.fl_str_mv |
fabiojreis@ufv.br |
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1817559860583071744 |