Achievement of constitutive fluorescent pLEXSY-egfp Leishmania braziliensis and its application as an alternative method for drug screening in vitro

Detalhes bibliográficos
Autor(a) principal: Bastos, Matheus Silva e
Data de Publicação: 2016
Outros Autores: Souza, Luciana Ângelo de, Onofre, Thiago Souza, Almeida, Márcia Rogéria de, Bressan, Gustavo Costa, Fietto, Juliana Lopes Rangel, Silva Júnior, Abelardo
Tipo de documento: Artigo
Idioma: eng
Título da fonte: LOCUS Repositório Institucional da UFV
Texto Completo: http://dx.doi.org/10.1590/0074-02760160237
http://www.locus.ufv.br/handle/123456789/12018
Resumo: Gene reporter-fluorescent cells have emerged as alternative method for drug screening. Achievement of constitutive expression of fluorescent protein GFP by Leishmania braziliensis as alternative method for drug screening. L. braziliensis-GFP was generated using Leishmania tarentolae pLEXSY-egfp for constitutive expression of GFP. Fluorescent cells were selected and subjected to standardisation tests of anti-promastigote and anti-intracellular amastigote assays. Our results showed that L. braziliensis-GFP method is faster and more sensitive than Allamar Blue-resazurin. Transfected parasites maintained stable fluorescence after successive in vitro passages and pLEXSY system can be used to achieve non-L. tarentolae fluorescent cells.
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spelling Achievement of constitutive fluorescent pLEXSY-egfp Leishmania braziliensis and its application as an alternative method for drug screening in vitroLeishmania braziliensisGreen fluorescent proteinDrug screeningGene reporter-fluorescent cells have emerged as alternative method for drug screening. Achievement of constitutive expression of fluorescent protein GFP by Leishmania braziliensis as alternative method for drug screening. L. braziliensis-GFP was generated using Leishmania tarentolae pLEXSY-egfp for constitutive expression of GFP. Fluorescent cells were selected and subjected to standardisation tests of anti-promastigote and anti-intracellular amastigote assays. Our results showed that L. braziliensis-GFP method is faster and more sensitive than Allamar Blue-resazurin. Transfected parasites maintained stable fluorescence after successive in vitro passages and pLEXSY system can be used to achieve non-L. tarentolae fluorescent cells.Memórias do Instituto Oswaldo Cruz2017-10-11T13:47:46Z2017-10-11T13:47:46Z2016-10-20info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articlepdfapplication/pdf1678-8060http://dx.doi.org/10.1590/0074-02760160237http://www.locus.ufv.br/handle/123456789/12018engvol.112 n.2, 155-159 Feb. 2017Bastos, Matheus Silva eSouza, Luciana Ângelo deOnofre, Thiago SouzaAlmeida, Márcia Rogéria deBressan, Gustavo CostaFietto, Juliana Lopes RangelSilva Júnior, Abelardoinfo:eu-repo/semantics/openAccessreponame:LOCUS Repositório Institucional da UFVinstname:Universidade Federal de Viçosa (UFV)instacron:UFV2024-07-12T08:22:07Zoai:locus.ufv.br:123456789/12018Repositório InstitucionalPUBhttps://www.locus.ufv.br/oai/requestfabiojreis@ufv.bropendoar:21452024-07-12T08:22:07LOCUS Repositório Institucional da UFV - Universidade Federal de Viçosa (UFV)false
dc.title.none.fl_str_mv Achievement of constitutive fluorescent pLEXSY-egfp Leishmania braziliensis and its application as an alternative method for drug screening in vitro
title Achievement of constitutive fluorescent pLEXSY-egfp Leishmania braziliensis and its application as an alternative method for drug screening in vitro
spellingShingle Achievement of constitutive fluorescent pLEXSY-egfp Leishmania braziliensis and its application as an alternative method for drug screening in vitro
Bastos, Matheus Silva e
Leishmania braziliensis
Green fluorescent protein
Drug screening
title_short Achievement of constitutive fluorescent pLEXSY-egfp Leishmania braziliensis and its application as an alternative method for drug screening in vitro
title_full Achievement of constitutive fluorescent pLEXSY-egfp Leishmania braziliensis and its application as an alternative method for drug screening in vitro
title_fullStr Achievement of constitutive fluorescent pLEXSY-egfp Leishmania braziliensis and its application as an alternative method for drug screening in vitro
title_full_unstemmed Achievement of constitutive fluorescent pLEXSY-egfp Leishmania braziliensis and its application as an alternative method for drug screening in vitro
title_sort Achievement of constitutive fluorescent pLEXSY-egfp Leishmania braziliensis and its application as an alternative method for drug screening in vitro
author Bastos, Matheus Silva e
author_facet Bastos, Matheus Silva e
Souza, Luciana Ângelo de
Onofre, Thiago Souza
Almeida, Márcia Rogéria de
Bressan, Gustavo Costa
Fietto, Juliana Lopes Rangel
Silva Júnior, Abelardo
author_role author
author2 Souza, Luciana Ângelo de
Onofre, Thiago Souza
Almeida, Márcia Rogéria de
Bressan, Gustavo Costa
Fietto, Juliana Lopes Rangel
Silva Júnior, Abelardo
author2_role author
author
author
author
author
author
dc.contributor.author.fl_str_mv Bastos, Matheus Silva e
Souza, Luciana Ângelo de
Onofre, Thiago Souza
Almeida, Márcia Rogéria de
Bressan, Gustavo Costa
Fietto, Juliana Lopes Rangel
Silva Júnior, Abelardo
dc.subject.por.fl_str_mv Leishmania braziliensis
Green fluorescent protein
Drug screening
topic Leishmania braziliensis
Green fluorescent protein
Drug screening
description Gene reporter-fluorescent cells have emerged as alternative method for drug screening. Achievement of constitutive expression of fluorescent protein GFP by Leishmania braziliensis as alternative method for drug screening. L. braziliensis-GFP was generated using Leishmania tarentolae pLEXSY-egfp for constitutive expression of GFP. Fluorescent cells were selected and subjected to standardisation tests of anti-promastigote and anti-intracellular amastigote assays. Our results showed that L. braziliensis-GFP method is faster and more sensitive than Allamar Blue-resazurin. Transfected parasites maintained stable fluorescence after successive in vitro passages and pLEXSY system can be used to achieve non-L. tarentolae fluorescent cells.
publishDate 2016
dc.date.none.fl_str_mv 2016-10-20
2017-10-11T13:47:46Z
2017-10-11T13:47:46Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv 1678-8060
http://dx.doi.org/10.1590/0074-02760160237
http://www.locus.ufv.br/handle/123456789/12018
identifier_str_mv 1678-8060
url http://dx.doi.org/10.1590/0074-02760160237
http://www.locus.ufv.br/handle/123456789/12018
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv vol.112 n.2, 155-159 Feb. 2017
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv pdf
application/pdf
dc.publisher.none.fl_str_mv Memórias do Instituto Oswaldo Cruz
publisher.none.fl_str_mv Memórias do Instituto Oswaldo Cruz
dc.source.none.fl_str_mv reponame:LOCUS Repositório Institucional da UFV
instname:Universidade Federal de Viçosa (UFV)
instacron:UFV
instname_str Universidade Federal de Viçosa (UFV)
instacron_str UFV
institution UFV
reponame_str LOCUS Repositório Institucional da UFV
collection LOCUS Repositório Institucional da UFV
repository.name.fl_str_mv LOCUS Repositório Institucional da UFV - Universidade Federal de Viçosa (UFV)
repository.mail.fl_str_mv fabiojreis@ufv.br
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