Potencial de controle biológico da ferrugem do cafeeiro com bactérias residentes do filoplano

Detalhes bibliográficos
Autor(a) principal: Godinho, Márcio Tadeu
Data de Publicação: 2010
Tipo de documento: Dissertação
Idioma: por
Título da fonte: LOCUS Repositório Institucional da UFV
Texto Completo: http://locus.ufv.br/handle/123456789/4380
Resumo: Looking for promising microorganisms for the control of coffee leaf rust, caused by Hemileia vastatrix, were isolated phylloplane residents from coffee leaves healthy by simply washing the sheet or by using ultrasound, with subsequent dilution. Were also selected bacteria capable of forming endospores, yielding a total of 217 isolates. After isolation were carried out four tests for selection of these organisms as biocontrol agents of rust. The first test conducted was the germination of urediniospores of H. vastatrix on glass slide, comparing the germination in distilled water and germination when a drop of each of the isolates obtained was deposited, from this experiment we selected 33 isolates with an inhibitory effect on germination. Subsequently, isolates were tested on leaf discs, as its capacity to suppress disease progression. The 33 isolates were sprayed on leaves of coffee plants at three different times in relation to inoculation with H. vastatrix (24 hours before, simultaneously and 24 hours later). Four isolates were considered capable of reducing the severity of the disease in the conditions of the experiment. These four isolates were tested on detached leaves to confirm the results, again observing reductions in disease severity compared to control, which contained only distilled water. The last experiment was conducted in green-house, comparing the four isolates with the controls (sprayed only with distilled water and with the systemic fungicide epoxiconazol). The four isolates provided the level of disease control similar to that obtained by the use of fungicide (approximately 100%). The identification based on fatty acid profile of the isolates identified UFV 025, UFV UFV 033 and 080 as Bacillus cereus, isolate UFV and 070 as Bacillus sphaericus. With the results of the experiments we can conclude that resident bacteria in the phylloplane of coffee can be isolated and selected as potential biocontrol agents of rust.
id UFV_e5d7155d58be734a90c5c23ae89337ed
oai_identifier_str oai:locus.ufv.br:123456789/4380
network_acronym_str UFV
network_name_str LOCUS Repositório Institucional da UFV
repository_id_str 2145
spelling Godinho, Márcio Tadeuhttp://lattes.cnpq.br/2989231021480966Bettiol, Wagnerhttp://buscatextual.cnpq.br/buscatextual/visualizacv.do?id=K4787836Z6Lopes, Everaldo Antoniohttp://buscatextual.cnpq.br/buscatextual/visualizacv.do?id=K4762607A4Romeiro, Reginaldo da Silvahttp://buscatextual.cnpq.br/buscatextual/visualizacv.do?id=K4783791J6Freitas, Leandro Grassi dehttp://buscatextual.cnpq.br/buscatextual/visualizacv.do?id=K4784897J6Oliveira, José Rogério dehttp://buscatextual.cnpq.br/buscatextual/visualizacv.do?id=K4785966E6Maffia, Luiz Antôniohttp://buscatextual.cnpq.br/buscatextual/visualizacv.do?id=K4783229P92015-03-26T13:37:42Z2011-10-142015-03-26T13:37:42Z2010-02-23GODINHO, Márcio Tadeu. Potential biological control of coffee rust with phylloplane resident bacteria. 2010. 47 f. Dissertação (Mestrado em Etiologia; Epidemiologia; Controle) - Universidade Federal de Viçosa, Viçosa, 2010.http://locus.ufv.br/handle/123456789/4380Looking for promising microorganisms for the control of coffee leaf rust, caused by Hemileia vastatrix, were isolated phylloplane residents from coffee leaves healthy by simply washing the sheet or by using ultrasound, with subsequent dilution. Were also selected bacteria capable of forming endospores, yielding a total of 217 isolates. After isolation were carried out four tests for selection of these organisms as biocontrol agents of rust. The first test conducted was the germination of urediniospores of H. vastatrix on glass slide, comparing the germination in distilled water and germination when a drop of each of the isolates obtained was deposited, from this experiment we selected 33 isolates with an inhibitory effect on germination. Subsequently, isolates were tested on leaf discs, as its capacity to suppress disease progression. The 33 isolates were sprayed on leaves of coffee plants at three different times in relation to inoculation with H. vastatrix (24 hours before, simultaneously and 24 hours later). Four isolates were considered capable of reducing the severity of the disease in the conditions of the experiment. These four isolates were tested on detached leaves to confirm the results, again observing reductions in disease severity compared to control, which contained only distilled water. The last experiment was conducted in green-house, comparing the four isolates with the controls (sprayed only with distilled water and with the systemic fungicide epoxiconazol). The four isolates provided the level of disease control similar to that obtained by the use of fungicide (approximately 100%). The identification based on fatty acid profile of the isolates identified UFV 025, UFV UFV 033 and 080 as Bacillus cereus, isolate UFV and 070 as Bacillus sphaericus. With the results of the experiments we can conclude that resident bacteria in the phylloplane of coffee can be isolated and selected as potential biocontrol agents of rust.Procurando-se microorganismos promissores para o controle da ferrugem do cafeeiro, causada por Hemileia vastatrix, foram isolados residentes do filoplano de folhas de café sadias por simples lavagem de folha ou por utilização de ultra-som, com posterior diluição. Também foram selecionadas bactérias capazes de formar endósporos, obtendo-se um total de 217 isolados. Após o isolamento foram realizados quatro ensaios para seleção destes organismos como agentes de biocontrole da ferrugem. O primeiro ensaio realizado foi de germinação de urediniósporos de H. vastatrix em lâmina de vidro, comparando-se a germinação em água destilada e a germinação quando foi depositada uma gota de cada um dos isolados obtidos, deste experimento foram selecionados 33 isolados com efeito inibitório na germinação. Posteriormente os isolados foram testados, em discos de folhas, quanto a sua capacidade em suprimir o avanço da doença. Os 33 isolados foram atomizados em folhas de cafeeiro em três diferentes tempos em relação à inoculação de H. vastatrix (24 horas antes, simultaneamente e 24 horas depois). Quatro isolados foram selecionados e considerados capazes de reduzir a severidade da doença nas condições do experimento. Esses quatro isolados foram testados em folhas destacadas para confirmação dos resultados, novamente observando-se redução na intensidade da doença em relação à testemunha, que continha apenas água destilada. O último experimento foi conduzido em casa-de-vegetação, comparando-se os quatro isolados com as testemunhas (pulverização apenas com água destilada e com o fungicida sistêmico epoxiconazol). Os quatro isolados proporcionaram o nível de controle da doença similar ao obtido pelo uso do fungicida (aproximadamente 100%). A identificação baseada no perfil de ácidos graxos identificou os isolados UFV 025, UFV 033 e UFV 080 como Bacillus cereus, e o isolado UFV 070 como Bacillus sphaericus. Com os resultados dos experimentos podemos concluir que bactérias residentes do filoplano de cafeeiro podem ser isoladas e selecionadas como potenciais agentes de biocontrole de ferrugemapplication/pdfporUniversidade Federal de ViçosaMestrado em FitopatologiaUFVBREtiologia; Epidemiologia; ControleControle biológicoHemileia vastatrixCaféFiloplanoFerrugemBiological controlHemileia vastatrixCoffeePhylloplaneRustCNPQ::CIENCIAS AGRARIAS::AGRONOMIA::FITOSSANIDADE::FITOPATOLOGIAPotencial de controle biológico da ferrugem do cafeeiro com bactérias residentes do filoplanoPotential biological control of coffee rust with phylloplane resident bacteriainfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/masterThesisinfo:eu-repo/semantics/openAccessreponame:LOCUS Repositório Institucional da UFVinstname:Universidade Federal de Viçosa (UFV)instacron:UFVORIGINALtexto completo.pdfapplication/pdf611413https://locus.ufv.br//bitstream/123456789/4380/1/texto%20completo.pdfd8119b46b042a6c0388e233d2daa1dccMD51TEXTtexto completo.pdf.txttexto completo.pdf.txtExtracted texttext/plain68841https://locus.ufv.br//bitstream/123456789/4380/2/texto%20completo.pdf.txt3fe2aa998a3d9325f4043a6d0821f433MD52THUMBNAILtexto completo.pdf.jpgtexto completo.pdf.jpgIM Thumbnailimage/jpeg3576https://locus.ufv.br//bitstream/123456789/4380/3/texto%20completo.pdf.jpgefe08c9fc187f5f5e7b1828f1533dd85MD53123456789/43802016-04-10 23:09:36.286oai:locus.ufv.br:123456789/4380Repositório InstitucionalPUBhttps://www.locus.ufv.br/oai/requestfabiojreis@ufv.bropendoar:21452016-04-11T02:09:36LOCUS Repositório Institucional da UFV - Universidade Federal de Viçosa (UFV)false
dc.title.por.fl_str_mv Potencial de controle biológico da ferrugem do cafeeiro com bactérias residentes do filoplano
dc.title.alternative.eng.fl_str_mv Potential biological control of coffee rust with phylloplane resident bacteria
title Potencial de controle biológico da ferrugem do cafeeiro com bactérias residentes do filoplano
spellingShingle Potencial de controle biológico da ferrugem do cafeeiro com bactérias residentes do filoplano
Godinho, Márcio Tadeu
Controle biológico
Hemileia vastatrix
Café
Filoplano
Ferrugem
Biological control
Hemileia vastatrix
Coffee
Phylloplane
Rust
CNPQ::CIENCIAS AGRARIAS::AGRONOMIA::FITOSSANIDADE::FITOPATOLOGIA
title_short Potencial de controle biológico da ferrugem do cafeeiro com bactérias residentes do filoplano
title_full Potencial de controle biológico da ferrugem do cafeeiro com bactérias residentes do filoplano
title_fullStr Potencial de controle biológico da ferrugem do cafeeiro com bactérias residentes do filoplano
title_full_unstemmed Potencial de controle biológico da ferrugem do cafeeiro com bactérias residentes do filoplano
title_sort Potencial de controle biológico da ferrugem do cafeeiro com bactérias residentes do filoplano
author Godinho, Márcio Tadeu
author_facet Godinho, Márcio Tadeu
author_role author
dc.contributor.authorLattes.por.fl_str_mv http://lattes.cnpq.br/2989231021480966
dc.contributor.author.fl_str_mv Godinho, Márcio Tadeu
dc.contributor.advisor-co1.fl_str_mv Bettiol, Wagner
dc.contributor.advisor-co1Lattes.fl_str_mv http://buscatextual.cnpq.br/buscatextual/visualizacv.do?id=K4787836Z6
dc.contributor.advisor-co2.fl_str_mv Lopes, Everaldo Antonio
dc.contributor.advisor-co2Lattes.fl_str_mv http://buscatextual.cnpq.br/buscatextual/visualizacv.do?id=K4762607A4
dc.contributor.advisor1.fl_str_mv Romeiro, Reginaldo da Silva
dc.contributor.advisor1Lattes.fl_str_mv http://buscatextual.cnpq.br/buscatextual/visualizacv.do?id=K4783791J6
dc.contributor.referee1.fl_str_mv Freitas, Leandro Grassi de
dc.contributor.referee1Lattes.fl_str_mv http://buscatextual.cnpq.br/buscatextual/visualizacv.do?id=K4784897J6
dc.contributor.referee2.fl_str_mv Oliveira, José Rogério de
dc.contributor.referee2Lattes.fl_str_mv http://buscatextual.cnpq.br/buscatextual/visualizacv.do?id=K4785966E6
dc.contributor.referee3.fl_str_mv Maffia, Luiz Antônio
dc.contributor.referee3Lattes.fl_str_mv http://buscatextual.cnpq.br/buscatextual/visualizacv.do?id=K4783229P9
contributor_str_mv Bettiol, Wagner
Lopes, Everaldo Antonio
Romeiro, Reginaldo da Silva
Freitas, Leandro Grassi de
Oliveira, José Rogério de
Maffia, Luiz Antônio
dc.subject.por.fl_str_mv Controle biológico
Hemileia vastatrix
Café
Filoplano
Ferrugem
topic Controle biológico
Hemileia vastatrix
Café
Filoplano
Ferrugem
Biological control
Hemileia vastatrix
Coffee
Phylloplane
Rust
CNPQ::CIENCIAS AGRARIAS::AGRONOMIA::FITOSSANIDADE::FITOPATOLOGIA
dc.subject.eng.fl_str_mv Biological control
Hemileia vastatrix
Coffee
Phylloplane
Rust
dc.subject.cnpq.fl_str_mv CNPQ::CIENCIAS AGRARIAS::AGRONOMIA::FITOSSANIDADE::FITOPATOLOGIA
description Looking for promising microorganisms for the control of coffee leaf rust, caused by Hemileia vastatrix, were isolated phylloplane residents from coffee leaves healthy by simply washing the sheet or by using ultrasound, with subsequent dilution. Were also selected bacteria capable of forming endospores, yielding a total of 217 isolates. After isolation were carried out four tests for selection of these organisms as biocontrol agents of rust. The first test conducted was the germination of urediniospores of H. vastatrix on glass slide, comparing the germination in distilled water and germination when a drop of each of the isolates obtained was deposited, from this experiment we selected 33 isolates with an inhibitory effect on germination. Subsequently, isolates were tested on leaf discs, as its capacity to suppress disease progression. The 33 isolates were sprayed on leaves of coffee plants at three different times in relation to inoculation with H. vastatrix (24 hours before, simultaneously and 24 hours later). Four isolates were considered capable of reducing the severity of the disease in the conditions of the experiment. These four isolates were tested on detached leaves to confirm the results, again observing reductions in disease severity compared to control, which contained only distilled water. The last experiment was conducted in green-house, comparing the four isolates with the controls (sprayed only with distilled water and with the systemic fungicide epoxiconazol). The four isolates provided the level of disease control similar to that obtained by the use of fungicide (approximately 100%). The identification based on fatty acid profile of the isolates identified UFV 025, UFV UFV 033 and 080 as Bacillus cereus, isolate UFV and 070 as Bacillus sphaericus. With the results of the experiments we can conclude that resident bacteria in the phylloplane of coffee can be isolated and selected as potential biocontrol agents of rust.
publishDate 2010
dc.date.issued.fl_str_mv 2010-02-23
dc.date.available.fl_str_mv 2011-10-14
2015-03-26T13:37:42Z
dc.date.accessioned.fl_str_mv 2015-03-26T13:37:42Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/masterThesis
format masterThesis
status_str publishedVersion
dc.identifier.citation.fl_str_mv GODINHO, Márcio Tadeu. Potential biological control of coffee rust with phylloplane resident bacteria. 2010. 47 f. Dissertação (Mestrado em Etiologia; Epidemiologia; Controle) - Universidade Federal de Viçosa, Viçosa, 2010.
dc.identifier.uri.fl_str_mv http://locus.ufv.br/handle/123456789/4380
identifier_str_mv GODINHO, Márcio Tadeu. Potential biological control of coffee rust with phylloplane resident bacteria. 2010. 47 f. Dissertação (Mestrado em Etiologia; Epidemiologia; Controle) - Universidade Federal de Viçosa, Viçosa, 2010.
url http://locus.ufv.br/handle/123456789/4380
dc.language.iso.fl_str_mv por
language por
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Universidade Federal de Viçosa
dc.publisher.program.fl_str_mv Mestrado em Fitopatologia
dc.publisher.initials.fl_str_mv UFV
dc.publisher.country.fl_str_mv BR
dc.publisher.department.fl_str_mv Etiologia; Epidemiologia; Controle
publisher.none.fl_str_mv Universidade Federal de Viçosa
dc.source.none.fl_str_mv reponame:LOCUS Repositório Institucional da UFV
instname:Universidade Federal de Viçosa (UFV)
instacron:UFV
instname_str Universidade Federal de Viçosa (UFV)
instacron_str UFV
institution UFV
reponame_str LOCUS Repositório Institucional da UFV
collection LOCUS Repositório Institucional da UFV
bitstream.url.fl_str_mv https://locus.ufv.br//bitstream/123456789/4380/1/texto%20completo.pdf
https://locus.ufv.br//bitstream/123456789/4380/2/texto%20completo.pdf.txt
https://locus.ufv.br//bitstream/123456789/4380/3/texto%20completo.pdf.jpg
bitstream.checksum.fl_str_mv d8119b46b042a6c0388e233d2daa1dcc
3fe2aa998a3d9325f4043a6d0821f433
efe08c9fc187f5f5e7b1828f1533dd85
bitstream.checksumAlgorithm.fl_str_mv MD5
MD5
MD5
repository.name.fl_str_mv LOCUS Repositório Institucional da UFV - Universidade Federal de Viçosa (UFV)
repository.mail.fl_str_mv fabiojreis@ufv.br
_version_ 1801213108011663360