Extracellular α-Galactosidase from Debaryomyces hansenii UFV-1 and Its use in the hydrolysis of raffinose oligosaccharides

Detalhes bibliográficos
Autor(a) principal: Rezende, Sebastião T. de
Data de Publicação: 2006
Outros Autores: Marques, Virgínia M., Trevizano, Larissa M., Passos, Flávia M. L., Oliveira, Maria G. A., Bemquerer, Marcelo P., Oliveira, Jamil S., Guimarães, Valéria M., Viana, Pollyanna A.
Tipo de documento: Artigo
Idioma: eng
Título da fonte: LOCUS Repositório Institucional da UFV
Texto Completo: http://dx.doi.org/10.1021/jf0526442
http://www.locus.ufv.br/handle/123456789/19902
Resumo: Raffinose oligosaccharides (RO) are the factors primarily responsible for flatulence upon ingestion of soybean-derived products. ROs are hydrolyzed by α-galactosidases that cleave α-1,6-linkages of α-galactoside residues. The objectives of this study were the purification and characterization of extracellular α-galactosidase from Debaryomyces hansenii UFV-1. The enzyme purified by gel filtration and anion exchange chromatographies presented an Mr value of 60 kDa and the N-terminal amino acid sequence YENGLNLVPQMGWN. The Km values for hydrolysis of pNPαGal, melibiose, stachyose, and raffinose were 0.30, 2.01, 9.66, and 16 mM, respectively. The α-galactosidase presented absolute specificity for galactose in the α-position, hydrolyzing pNPGal, stachyose, raffinose, melibiose, and polymers. The enzyme was noncompetitively inhibited by galactose (Ki = 2.7 mM) and melibiose (Ki = 1.2 mM). Enzyme treatments of soy milk for 4 h at 60 °C reduced the amounts of stachyose and raffinose by 100%.
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spelling Extracellular α-Galactosidase from Debaryomyces hansenii UFV-1 and Its use in the hydrolysis of raffinose oligosaccharidesα-GalactosidaseDebaryomyces hansenii UFV-1Raffinose oligosaccharidesCharacterizationFlatulenceRaffinose oligosaccharides (RO) are the factors primarily responsible for flatulence upon ingestion of soybean-derived products. ROs are hydrolyzed by α-galactosidases that cleave α-1,6-linkages of α-galactoside residues. The objectives of this study were the purification and characterization of extracellular α-galactosidase from Debaryomyces hansenii UFV-1. The enzyme purified by gel filtration and anion exchange chromatographies presented an Mr value of 60 kDa and the N-terminal amino acid sequence YENGLNLVPQMGWN. The Km values for hydrolysis of pNPαGal, melibiose, stachyose, and raffinose were 0.30, 2.01, 9.66, and 16 mM, respectively. The α-galactosidase presented absolute specificity for galactose in the α-position, hydrolyzing pNPGal, stachyose, raffinose, melibiose, and polymers. The enzyme was noncompetitively inhibited by galactose (Ki = 2.7 mM) and melibiose (Ki = 1.2 mM). Enzyme treatments of soy milk for 4 h at 60 °C reduced the amounts of stachyose and raffinose by 100%.Journal of Agricultural and Food Chemistry2018-06-04T12:00:06Z2018-06-04T12:00:06Z2006-02-17info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articlepdfapplication/pdf15205118http://dx.doi.org/10.1021/jf0526442http://www.locus.ufv.br/handle/123456789/19902engv. 54, n. 6, p. 2385–2391, Fevereiro 2006American Chemical Societyinfo:eu-repo/semantics/openAccessRezende, Sebastião T. deMarques, Virgínia M.Trevizano, Larissa M.Passos, Flávia M. L.Oliveira, Maria G. A.Bemquerer, Marcelo P.Oliveira, Jamil S.Guimarães, Valéria M.Viana, Pollyanna A.reponame:LOCUS Repositório Institucional da UFVinstname:Universidade Federal de Viçosa (UFV)instacron:UFV2024-07-12T06:41:47Zoai:locus.ufv.br:123456789/19902Repositório InstitucionalPUBhttps://www.locus.ufv.br/oai/requestfabiojreis@ufv.bropendoar:21452024-07-12T06:41:47LOCUS Repositório Institucional da UFV - Universidade Federal de Viçosa (UFV)false
dc.title.none.fl_str_mv Extracellular α-Galactosidase from Debaryomyces hansenii UFV-1 and Its use in the hydrolysis of raffinose oligosaccharides
title Extracellular α-Galactosidase from Debaryomyces hansenii UFV-1 and Its use in the hydrolysis of raffinose oligosaccharides
spellingShingle Extracellular α-Galactosidase from Debaryomyces hansenii UFV-1 and Its use in the hydrolysis of raffinose oligosaccharides
Rezende, Sebastião T. de
α-Galactosidase
Debaryomyces hansenii UFV-1
Raffinose oligosaccharides
Characterization
Flatulence
title_short Extracellular α-Galactosidase from Debaryomyces hansenii UFV-1 and Its use in the hydrolysis of raffinose oligosaccharides
title_full Extracellular α-Galactosidase from Debaryomyces hansenii UFV-1 and Its use in the hydrolysis of raffinose oligosaccharides
title_fullStr Extracellular α-Galactosidase from Debaryomyces hansenii UFV-1 and Its use in the hydrolysis of raffinose oligosaccharides
title_full_unstemmed Extracellular α-Galactosidase from Debaryomyces hansenii UFV-1 and Its use in the hydrolysis of raffinose oligosaccharides
title_sort Extracellular α-Galactosidase from Debaryomyces hansenii UFV-1 and Its use in the hydrolysis of raffinose oligosaccharides
author Rezende, Sebastião T. de
author_facet Rezende, Sebastião T. de
Marques, Virgínia M.
Trevizano, Larissa M.
Passos, Flávia M. L.
Oliveira, Maria G. A.
Bemquerer, Marcelo P.
Oliveira, Jamil S.
Guimarães, Valéria M.
Viana, Pollyanna A.
author_role author
author2 Marques, Virgínia M.
Trevizano, Larissa M.
Passos, Flávia M. L.
Oliveira, Maria G. A.
Bemquerer, Marcelo P.
Oliveira, Jamil S.
Guimarães, Valéria M.
Viana, Pollyanna A.
author2_role author
author
author
author
author
author
author
author
dc.contributor.author.fl_str_mv Rezende, Sebastião T. de
Marques, Virgínia M.
Trevizano, Larissa M.
Passos, Flávia M. L.
Oliveira, Maria G. A.
Bemquerer, Marcelo P.
Oliveira, Jamil S.
Guimarães, Valéria M.
Viana, Pollyanna A.
dc.subject.por.fl_str_mv α-Galactosidase
Debaryomyces hansenii UFV-1
Raffinose oligosaccharides
Characterization
Flatulence
topic α-Galactosidase
Debaryomyces hansenii UFV-1
Raffinose oligosaccharides
Characterization
Flatulence
description Raffinose oligosaccharides (RO) are the factors primarily responsible for flatulence upon ingestion of soybean-derived products. ROs are hydrolyzed by α-galactosidases that cleave α-1,6-linkages of α-galactoside residues. The objectives of this study were the purification and characterization of extracellular α-galactosidase from Debaryomyces hansenii UFV-1. The enzyme purified by gel filtration and anion exchange chromatographies presented an Mr value of 60 kDa and the N-terminal amino acid sequence YENGLNLVPQMGWN. The Km values for hydrolysis of pNPαGal, melibiose, stachyose, and raffinose were 0.30, 2.01, 9.66, and 16 mM, respectively. The α-galactosidase presented absolute specificity for galactose in the α-position, hydrolyzing pNPGal, stachyose, raffinose, melibiose, and polymers. The enzyme was noncompetitively inhibited by galactose (Ki = 2.7 mM) and melibiose (Ki = 1.2 mM). Enzyme treatments of soy milk for 4 h at 60 °C reduced the amounts of stachyose and raffinose by 100%.
publishDate 2006
dc.date.none.fl_str_mv 2006-02-17
2018-06-04T12:00:06Z
2018-06-04T12:00:06Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv 15205118
http://dx.doi.org/10.1021/jf0526442
http://www.locus.ufv.br/handle/123456789/19902
identifier_str_mv 15205118
url http://dx.doi.org/10.1021/jf0526442
http://www.locus.ufv.br/handle/123456789/19902
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv v. 54, n. 6, p. 2385–2391, Fevereiro 2006
dc.rights.driver.fl_str_mv American Chemical Society
info:eu-repo/semantics/openAccess
rights_invalid_str_mv American Chemical Society
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv pdf
application/pdf
dc.publisher.none.fl_str_mv Journal of Agricultural and Food Chemistry
publisher.none.fl_str_mv Journal of Agricultural and Food Chemistry
dc.source.none.fl_str_mv reponame:LOCUS Repositório Institucional da UFV
instname:Universidade Federal de Viçosa (UFV)
instacron:UFV
instname_str Universidade Federal de Viçosa (UFV)
instacron_str UFV
institution UFV
reponame_str LOCUS Repositório Institucional da UFV
collection LOCUS Repositório Institucional da UFV
repository.name.fl_str_mv LOCUS Repositório Institucional da UFV - Universidade Federal de Viçosa (UFV)
repository.mail.fl_str_mv fabiojreis@ufv.br
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