Detection of three Allexivirus species infecting garlic in Brazil

Detalhes bibliográficos
Autor(a) principal: Melo Filho, Péricles de Albuquerque
Data de Publicação: 2004
Outros Autores: Nagata, Tatsuya, Dusi, André Nepomuceno, Buso, José Amauri, Torres, Antonio Carlos, Eiras, Marcelo, Resende, Renato de Oliveira
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da UnB
Texto Completo: http://repositorio.unb.br/handle/10482/26161
https://dx.doi.org/10.1590/S0100-204X2004000800002
Resumo: Garlic viruses often occur in mixed infections under field conditions. In this study, garlic samples collected in three geographical areas of Brazil were tested by Dot-ELISA for the detection of allexiviruses using monoclonal specific antibodies to detect Garlic virus A (GarV-A), Garlic virus B (GarV-B), Garlic virus C (GarV-C) and a polyclonal antiserum able to detect the three virus species mentioned plus Garlic virus D (GarV-D). The detected viruses were biologically isolated by successive passages through Chenopodium quinoa. Reverse Transcriptase Polimerase Chain Reaction (RT-PCR) was performed using primers designed from specific regions of the coat protein genes of Japanese allexiviruses available in the Genetic Bank of National Center of Biotechnology Information (NCBI). By these procedures, individual garlic virus genomes were isolated and sequenced. The nucleotide and amino acid sequence analysis and the one with serological data revealed the presence of three distinct allexiviruses GarV-C, GarV-D and a recently described allexivirus, named Garlic mite-borne filamentous virus (GarMbFV), in Brazil.
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spelling Detection of three Allexivirus species infecting garlic in BrazilDetecção de três espécies de Allexivirus que infectam o alho no BrasilAllium sativumVírus - detecçãoProteínas capsidiaisDot-ELISART-PCRGarlic viruses often occur in mixed infections under field conditions. In this study, garlic samples collected in three geographical areas of Brazil were tested by Dot-ELISA for the detection of allexiviruses using monoclonal specific antibodies to detect Garlic virus A (GarV-A), Garlic virus B (GarV-B), Garlic virus C (GarV-C) and a polyclonal antiserum able to detect the three virus species mentioned plus Garlic virus D (GarV-D). The detected viruses were biologically isolated by successive passages through Chenopodium quinoa. Reverse Transcriptase Polimerase Chain Reaction (RT-PCR) was performed using primers designed from specific regions of the coat protein genes of Japanese allexiviruses available in the Genetic Bank of National Center of Biotechnology Information (NCBI). By these procedures, individual garlic virus genomes were isolated and sequenced. The nucleotide and amino acid sequence analysis and the one with serological data revealed the presence of three distinct allexiviruses GarV-C, GarV-D and a recently described allexivirus, named Garlic mite-borne filamentous virus (GarMbFV), in Brazil.Infecções virais em alho são normalmente causadas por um complexo viral. Neste estudo, um complexo viral de alho, coletado em campo, em três regiões geográficas, foi testado com anti-soros monoclonais específicos para Garlic virus A (GarV-A), Garlic virus B (GarV-B), Garlic virus C (GarV-C) e um anti-soro policlonal capaz de detectar os três vírus mencionados e Garlic virus D (GarV-D). Procedeu-se à amplificação por transcriptase reversa-reação em cadeia da polimerase (RT-PCR) usando oligonucleotídeos sintetizados a partir de regiões específicas de genes de proteínas capsidiais de allexivirus japoneses e disponíveis no GeneBank (National Center of Biotechnology Information - NCBI). Por esse procedimento, vírus individuais foram isolados e seqüenciados. Os vírus detectados foram biologicamente isolados por meio de sucessivas inoculações em Chenopodium quinoa. A análise das seqüências de nucleotídeos, de aminoácidos e os resultados sorológicos revelaram a presença de três espécies distintas de allexivirus GarV-C, GarV-D e Garlic mite-borne filamentous virus (GarMbFV) no Brasil.Em processamentoEmbrapa Informação TecnológicaPesquisa Agropecuária Brasileira2017-12-07T04:39:14Z2017-12-07T04:39:14Z2004info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfPesq. agropec. bras.,v.39,n.8,p.735-740,2004http://repositorio.unb.br/handle/10482/26161https://dx.doi.org/10.1590/S0100-204X2004000800002Melo Filho, Péricles de AlbuquerqueNagata, TatsuyaDusi, André NepomucenoBuso, José AmauriTorres, Antonio CarlosEiras, MarceloResende, Renato de Oliveirainfo:eu-repo/semantics/openAccessengreponame:Repositório Institucional da UnBinstname:Universidade de Brasília (UnB)instacron:UNB2024-08-28T16:17:04Zoai:repositorio.unb.br:10482/26161Repositório InstitucionalPUBhttps://repositorio.unb.br/oai/requestrepositorio@unb.bropendoar:2024-08-28T16:17:04Repositório Institucional da UnB - Universidade de Brasília (UnB)false
dc.title.none.fl_str_mv Detection of three Allexivirus species infecting garlic in Brazil
Detecção de três espécies de Allexivirus que infectam o alho no Brasil
title Detection of three Allexivirus species infecting garlic in Brazil
spellingShingle Detection of three Allexivirus species infecting garlic in Brazil
Melo Filho, Péricles de Albuquerque
Allium sativum
Vírus - detecção
Proteínas capsidiais
Dot-ELISA
RT-PCR
title_short Detection of three Allexivirus species infecting garlic in Brazil
title_full Detection of three Allexivirus species infecting garlic in Brazil
title_fullStr Detection of three Allexivirus species infecting garlic in Brazil
title_full_unstemmed Detection of three Allexivirus species infecting garlic in Brazil
title_sort Detection of three Allexivirus species infecting garlic in Brazil
author Melo Filho, Péricles de Albuquerque
author_facet Melo Filho, Péricles de Albuquerque
Nagata, Tatsuya
Dusi, André Nepomuceno
Buso, José Amauri
Torres, Antonio Carlos
Eiras, Marcelo
Resende, Renato de Oliveira
author_role author
author2 Nagata, Tatsuya
Dusi, André Nepomuceno
Buso, José Amauri
Torres, Antonio Carlos
Eiras, Marcelo
Resende, Renato de Oliveira
author2_role author
author
author
author
author
author
dc.contributor.author.fl_str_mv Melo Filho, Péricles de Albuquerque
Nagata, Tatsuya
Dusi, André Nepomuceno
Buso, José Amauri
Torres, Antonio Carlos
Eiras, Marcelo
Resende, Renato de Oliveira
dc.subject.por.fl_str_mv Allium sativum
Vírus - detecção
Proteínas capsidiais
Dot-ELISA
RT-PCR
topic Allium sativum
Vírus - detecção
Proteínas capsidiais
Dot-ELISA
RT-PCR
description Garlic viruses often occur in mixed infections under field conditions. In this study, garlic samples collected in three geographical areas of Brazil were tested by Dot-ELISA for the detection of allexiviruses using monoclonal specific antibodies to detect Garlic virus A (GarV-A), Garlic virus B (GarV-B), Garlic virus C (GarV-C) and a polyclonal antiserum able to detect the three virus species mentioned plus Garlic virus D (GarV-D). The detected viruses were biologically isolated by successive passages through Chenopodium quinoa. Reverse Transcriptase Polimerase Chain Reaction (RT-PCR) was performed using primers designed from specific regions of the coat protein genes of Japanese allexiviruses available in the Genetic Bank of National Center of Biotechnology Information (NCBI). By these procedures, individual garlic virus genomes were isolated and sequenced. The nucleotide and amino acid sequence analysis and the one with serological data revealed the presence of three distinct allexiviruses GarV-C, GarV-D and a recently described allexivirus, named Garlic mite-borne filamentous virus (GarMbFV), in Brazil.
publishDate 2004
dc.date.none.fl_str_mv 2004
2017-12-07T04:39:14Z
2017-12-07T04:39:14Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv Pesq. agropec. bras.,v.39,n.8,p.735-740,2004
http://repositorio.unb.br/handle/10482/26161
https://dx.doi.org/10.1590/S0100-204X2004000800002
identifier_str_mv Pesq. agropec. bras.,v.39,n.8,p.735-740,2004
url http://repositorio.unb.br/handle/10482/26161
https://dx.doi.org/10.1590/S0100-204X2004000800002
dc.language.iso.fl_str_mv eng
language eng
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Embrapa Informação TecnológicaPesquisa Agropecuária Brasileira
publisher.none.fl_str_mv Embrapa Informação TecnológicaPesquisa Agropecuária Brasileira
dc.source.none.fl_str_mv reponame:Repositório Institucional da UnB
instname:Universidade de Brasília (UnB)
instacron:UNB
instname_str Universidade de Brasília (UnB)
instacron_str UNB
institution UNB
reponame_str Repositório Institucional da UnB
collection Repositório Institucional da UnB
repository.name.fl_str_mv Repositório Institucional da UnB - Universidade de Brasília (UnB)
repository.mail.fl_str_mv repositorio@unb.br
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