Prevalence of human papillomavirus types in women with pre-neoplastic and neoplastic cervical lesions in the Federal District of Brazil

Detalhes bibliográficos
Autor(a) principal: Camara, Geni NL
Data de Publicação: 2003
Outros Autores: Cerqueira, Daniela M, Oliveira, Ana PG, Silva, Evandro O, Carvalho, Luciano GS, Martins, Cláudia RF
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da UnB
Texto Completo: http://repositorio.unb.br/handle/10482/25984
https://dx.doi.org/10.1590/S0074-02762003000700003
Resumo: As a contribution to the public health authorities in planning prophylactic and therapeutic vaccine strategies, we describe the prevalence of human papillomavirus (HPV) types in women presenting abnormal cytological results in Pap smear screening tests in the Federal District, Central Brazil. We studied 129 cervical scraping samples from women whose cytological tests showed either pre-neoplastic or neoplastic lesions. Amplification of HPV DNA was performed by polymerase chain reaction using consensus primers MY09 and MY11 followed by identification of isolates by restriction fragment length polymorphism. We detected HPV DNA in 62% of the samples, including HPV-16 in 43.8%, HPV-58 in 12.5%, HPV-31 in 10%, HPV-53 in 6.3%, each of HPV-18 and HPV-33 in 3.8% of the isolates. Other types (HPV-35, -52, -66, -CP8304, -6, -11, and -CP8061) were less frequent (= or < 2.5% each). The prevalence of HPV-58 was relatively higher in this population than in data in South America, but similar to results obtained in other studies in Latin America, Europe, and Eastern Asia. Case-control studies need to be carried out to establish the association between the prevalence of HPV types specially the less frequent high-risk types and cervical cancer.
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spelling Prevalence of human papillomavirus types in women with pre-neoplastic and neoplastic cervical lesions in the Federal District of BrazilPapilomavírus humanoGenótiposCervical - lesãoAs a contribution to the public health authorities in planning prophylactic and therapeutic vaccine strategies, we describe the prevalence of human papillomavirus (HPV) types in women presenting abnormal cytological results in Pap smear screening tests in the Federal District, Central Brazil. We studied 129 cervical scraping samples from women whose cytological tests showed either pre-neoplastic or neoplastic lesions. Amplification of HPV DNA was performed by polymerase chain reaction using consensus primers MY09 and MY11 followed by identification of isolates by restriction fragment length polymorphism. We detected HPV DNA in 62% of the samples, including HPV-16 in 43.8%, HPV-58 in 12.5%, HPV-31 in 10%, HPV-53 in 6.3%, each of HPV-18 and HPV-33 in 3.8% of the isolates. Other types (HPV-35, -52, -66, -CP8304, -6, -11, and -CP8061) were less frequent (= or < 2.5% each). The prevalence of HPV-58 was relatively higher in this population than in data in South America, but similar to results obtained in other studies in Latin America, Europe, and Eastern Asia. Case-control studies need to be carried out to establish the association between the prevalence of HPV types specially the less frequent high-risk types and cervical cancer.Em processamentoInstituto Oswaldo Cruz, Ministério da Saúde2017-12-07T04:37:36Z2017-12-07T04:37:36Z2003info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfMem. Inst. Oswaldo Cruz,v.98,n.7,p.879-883,2003http://repositorio.unb.br/handle/10482/25984https://dx.doi.org/10.1590/S0074-02762003000700003Camara, Geni NLCerqueira, Daniela MOliveira, Ana PGSilva, Evandro OCarvalho, Luciano GSMartins, Cláudia RFinfo:eu-repo/semantics/openAccessengreponame:Repositório Institucional da UnBinstname:Universidade de Brasília (UnB)instacron:UNB2024-08-28T16:31:55Zoai:repositorio.unb.br:10482/25984Repositório InstitucionalPUBhttps://repositorio.unb.br/oai/requestrepositorio@unb.bropendoar:2024-08-28T16:31:55Repositório Institucional da UnB - Universidade de Brasília (UnB)false
dc.title.none.fl_str_mv Prevalence of human papillomavirus types in women with pre-neoplastic and neoplastic cervical lesions in the Federal District of Brazil
title Prevalence of human papillomavirus types in women with pre-neoplastic and neoplastic cervical lesions in the Federal District of Brazil
spellingShingle Prevalence of human papillomavirus types in women with pre-neoplastic and neoplastic cervical lesions in the Federal District of Brazil
Camara, Geni NL
Papilomavírus humano
Genótipos
Cervical - lesão
title_short Prevalence of human papillomavirus types in women with pre-neoplastic and neoplastic cervical lesions in the Federal District of Brazil
title_full Prevalence of human papillomavirus types in women with pre-neoplastic and neoplastic cervical lesions in the Federal District of Brazil
title_fullStr Prevalence of human papillomavirus types in women with pre-neoplastic and neoplastic cervical lesions in the Federal District of Brazil
title_full_unstemmed Prevalence of human papillomavirus types in women with pre-neoplastic and neoplastic cervical lesions in the Federal District of Brazil
title_sort Prevalence of human papillomavirus types in women with pre-neoplastic and neoplastic cervical lesions in the Federal District of Brazil
author Camara, Geni NL
author_facet Camara, Geni NL
Cerqueira, Daniela M
Oliveira, Ana PG
Silva, Evandro O
Carvalho, Luciano GS
Martins, Cláudia RF
author_role author
author2 Cerqueira, Daniela M
Oliveira, Ana PG
Silva, Evandro O
Carvalho, Luciano GS
Martins, Cláudia RF
author2_role author
author
author
author
author
dc.contributor.author.fl_str_mv Camara, Geni NL
Cerqueira, Daniela M
Oliveira, Ana PG
Silva, Evandro O
Carvalho, Luciano GS
Martins, Cláudia RF
dc.subject.por.fl_str_mv Papilomavírus humano
Genótipos
Cervical - lesão
topic Papilomavírus humano
Genótipos
Cervical - lesão
description As a contribution to the public health authorities in planning prophylactic and therapeutic vaccine strategies, we describe the prevalence of human papillomavirus (HPV) types in women presenting abnormal cytological results in Pap smear screening tests in the Federal District, Central Brazil. We studied 129 cervical scraping samples from women whose cytological tests showed either pre-neoplastic or neoplastic lesions. Amplification of HPV DNA was performed by polymerase chain reaction using consensus primers MY09 and MY11 followed by identification of isolates by restriction fragment length polymorphism. We detected HPV DNA in 62% of the samples, including HPV-16 in 43.8%, HPV-58 in 12.5%, HPV-31 in 10%, HPV-53 in 6.3%, each of HPV-18 and HPV-33 in 3.8% of the isolates. Other types (HPV-35, -52, -66, -CP8304, -6, -11, and -CP8061) were less frequent (= or < 2.5% each). The prevalence of HPV-58 was relatively higher in this population than in data in South America, but similar to results obtained in other studies in Latin America, Europe, and Eastern Asia. Case-control studies need to be carried out to establish the association between the prevalence of HPV types specially the less frequent high-risk types and cervical cancer.
publishDate 2003
dc.date.none.fl_str_mv 2003
2017-12-07T04:37:36Z
2017-12-07T04:37:36Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv Mem. Inst. Oswaldo Cruz,v.98,n.7,p.879-883,2003
http://repositorio.unb.br/handle/10482/25984
https://dx.doi.org/10.1590/S0074-02762003000700003
identifier_str_mv Mem. Inst. Oswaldo Cruz,v.98,n.7,p.879-883,2003
url http://repositorio.unb.br/handle/10482/25984
https://dx.doi.org/10.1590/S0074-02762003000700003
dc.language.iso.fl_str_mv eng
language eng
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Instituto Oswaldo Cruz, Ministério da Saúde
publisher.none.fl_str_mv Instituto Oswaldo Cruz, Ministério da Saúde
dc.source.none.fl_str_mv reponame:Repositório Institucional da UnB
instname:Universidade de Brasília (UnB)
instacron:UNB
instname_str Universidade de Brasília (UnB)
instacron_str UNB
institution UNB
reponame_str Repositório Institucional da UnB
collection Repositório Institucional da UnB
repository.name.fl_str_mv Repositório Institucional da UnB - Universidade de Brasília (UnB)
repository.mail.fl_str_mv repositorio@unb.br
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