Garlic viral complex: identification of Potyviruses and Carlavirus in Central Brazil

Detalhes bibliográficos
Autor(a) principal: FAJARDO, THOR V. M.
Data de Publicação: 2001
Outros Autores: NISHIJIMA, MARTA, BUSO, JOSÉ A., TORRES, ANTÔNIO C., ÁVILA, ANTÔNIO C., RESENDE, RENATO O.
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da UnB
Texto Completo: http://repositorio.unb.br/handle/10482/25724
https://dx.doi.org/10.1590/S0100-41582001000300007
Resumo: Garlic viruses often occur in complex infections in nature. In this study, a garlic virus complex, collected in fields in Brazil, was purified. RT-PCR was performed using specific primers designed from the consensus regions of the coat protein genes of Onion yellow dwarf virus, a garlic strain (OYDV-G) and Leek yellow stripe virus (LYSV). cDNA of Garlic common latent virus (GCLV) was synthesized using oligo-dT and random primers. By these procedures individual garlic virus genomes were isolated and sequenced. The nucleotide sequence analysis associated with serological data reveals the presence of two Potyvirus OYDV-G and LYSV, and GCLV, a Carlavirus, simultaneously infecting garlic plants. Deduced amino acid sequences of the Brazilian isolates were compared with related viruses reported in different geographical regions of the world. The analysis showed closed relations considering the Brazilian isolates of OYDV-G and GCLV, and large divergence considering LYSV isolate. The detection of these virus species was confirmed by specific reactions observed when coat protein genes of the Brazilian isolates were used as probes in dot-blot and Southern blot hybridization assays. In field natural viral re-infection of virus-free garlic was evaluated.
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spelling Garlic viral complex: identification of Potyviruses and Carlavirus in Central BrazilComplexo viral do alho: identificação de Potyvirus e Carlavirus na região central do BrasilOydv-glysvgclvDetecçãoProteína de revestimentoPCRSondasEpidemiologiaGarlic viruses often occur in complex infections in nature. In this study, a garlic virus complex, collected in fields in Brazil, was purified. RT-PCR was performed using specific primers designed from the consensus regions of the coat protein genes of Onion yellow dwarf virus, a garlic strain (OYDV-G) and Leek yellow stripe virus (LYSV). cDNA of Garlic common latent virus (GCLV) was synthesized using oligo-dT and random primers. By these procedures individual garlic virus genomes were isolated and sequenced. The nucleotide sequence analysis associated with serological data reveals the presence of two Potyvirus OYDV-G and LYSV, and GCLV, a Carlavirus, simultaneously infecting garlic plants. Deduced amino acid sequences of the Brazilian isolates were compared with related viruses reported in different geographical regions of the world. The analysis showed closed relations considering the Brazilian isolates of OYDV-G and GCLV, and large divergence considering LYSV isolate. The detection of these virus species was confirmed by specific reactions observed when coat protein genes of the Brazilian isolates were used as probes in dot-blot and Southern blot hybridization assays. In field natural viral re-infection of virus-free garlic was evaluated.Infecções virais em alho são normalmente causadas por um complexo viral. Neste estudo, um complexo viral de alho, coletado em campo, foi purificado. Procedeu-se à amplificação por RT-PCR usando oligonucleotídeos desenhados para regiões-consenso dos genes das proteínas capsidiais de Onion yellow dwarf virus, estirpe do alho e de Leek yellow stripe virus. cDNA de Garlic common latent virus foi sintetizado usando oligo-dT e oligonucleotídeos aleatórios. Por estes procedimentos clones de diferentes espécies virais foram isolados e sequenciados. A análise das sequências nucleotídicas e os resultados sorológicos revelaram a presença dos Potyvirus OYDV-G e LYSV e do Carlavirus GCLV, simultaneamente infetando plantas de alho. As seqüências de aminoácidos deduzidos dos isolados brasileiros foram comparadas com aquelas de vírus relacionados, relatados em diferentes regiões do mundo. A análise mostrou pequena variabilidade em relação aos isolados brasileiros de OYDV-G e GCLV, e maior divergência em relação ao isolado de LYSV. A detecção destas espécies virais também foi obtida por reações específicas observadas quando o gene da proteína capsídica dos isolados brasileiros foi usado como sonda em ensaios de hibridização do tipo dot-blot e Southern blot. Em campo, a re-infecção natural de alho livre de vírus foi avaliada.Em processamentoSociedade Brasileira de Fitopatologia2017-12-07T04:33:54Z2017-12-07T04:33:54Z2001info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfFitopatol. bras.,v.26,n.3,p.619-626,2001http://repositorio.unb.br/handle/10482/25724https://dx.doi.org/10.1590/S0100-41582001000300007FAJARDO, THOR V. M.NISHIJIMA, MARTABUSO, JOSÉ A.TORRES, ANTÔNIO C.ÁVILA, ANTÔNIO C.RESENDE, RENATO O.info:eu-repo/semantics/openAccessengreponame:Repositório Institucional da UnBinstname:Universidade de Brasília (UnB)instacron:UNB2024-08-28T19:04:08Zoai:repositorio.unb.br:10482/25724Repositório InstitucionalPUBhttps://repositorio.unb.br/oai/requestrepositorio@unb.bropendoar:2024-08-28T19:04:08Repositório Institucional da UnB - Universidade de Brasília (UnB)false
dc.title.none.fl_str_mv Garlic viral complex: identification of Potyviruses and Carlavirus in Central Brazil
Complexo viral do alho: identificação de Potyvirus e Carlavirus na região central do Brasil
title Garlic viral complex: identification of Potyviruses and Carlavirus in Central Brazil
spellingShingle Garlic viral complex: identification of Potyviruses and Carlavirus in Central Brazil
FAJARDO, THOR V. M.
Oydv-g
lysv
gclv
Detecção
Proteína de revestimento
PCR
Sondas
Epidemiologia
title_short Garlic viral complex: identification of Potyviruses and Carlavirus in Central Brazil
title_full Garlic viral complex: identification of Potyviruses and Carlavirus in Central Brazil
title_fullStr Garlic viral complex: identification of Potyviruses and Carlavirus in Central Brazil
title_full_unstemmed Garlic viral complex: identification of Potyviruses and Carlavirus in Central Brazil
title_sort Garlic viral complex: identification of Potyviruses and Carlavirus in Central Brazil
author FAJARDO, THOR V. M.
author_facet FAJARDO, THOR V. M.
NISHIJIMA, MARTA
BUSO, JOSÉ A.
TORRES, ANTÔNIO C.
ÁVILA, ANTÔNIO C.
RESENDE, RENATO O.
author_role author
author2 NISHIJIMA, MARTA
BUSO, JOSÉ A.
TORRES, ANTÔNIO C.
ÁVILA, ANTÔNIO C.
RESENDE, RENATO O.
author2_role author
author
author
author
author
dc.contributor.author.fl_str_mv FAJARDO, THOR V. M.
NISHIJIMA, MARTA
BUSO, JOSÉ A.
TORRES, ANTÔNIO C.
ÁVILA, ANTÔNIO C.
RESENDE, RENATO O.
dc.subject.por.fl_str_mv Oydv-g
lysv
gclv
Detecção
Proteína de revestimento
PCR
Sondas
Epidemiologia
topic Oydv-g
lysv
gclv
Detecção
Proteína de revestimento
PCR
Sondas
Epidemiologia
description Garlic viruses often occur in complex infections in nature. In this study, a garlic virus complex, collected in fields in Brazil, was purified. RT-PCR was performed using specific primers designed from the consensus regions of the coat protein genes of Onion yellow dwarf virus, a garlic strain (OYDV-G) and Leek yellow stripe virus (LYSV). cDNA of Garlic common latent virus (GCLV) was synthesized using oligo-dT and random primers. By these procedures individual garlic virus genomes were isolated and sequenced. The nucleotide sequence analysis associated with serological data reveals the presence of two Potyvirus OYDV-G and LYSV, and GCLV, a Carlavirus, simultaneously infecting garlic plants. Deduced amino acid sequences of the Brazilian isolates were compared with related viruses reported in different geographical regions of the world. The analysis showed closed relations considering the Brazilian isolates of OYDV-G and GCLV, and large divergence considering LYSV isolate. The detection of these virus species was confirmed by specific reactions observed when coat protein genes of the Brazilian isolates were used as probes in dot-blot and Southern blot hybridization assays. In field natural viral re-infection of virus-free garlic was evaluated.
publishDate 2001
dc.date.none.fl_str_mv 2001
2017-12-07T04:33:54Z
2017-12-07T04:33:54Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv Fitopatol. bras.,v.26,n.3,p.619-626,2001
http://repositorio.unb.br/handle/10482/25724
https://dx.doi.org/10.1590/S0100-41582001000300007
identifier_str_mv Fitopatol. bras.,v.26,n.3,p.619-626,2001
url http://repositorio.unb.br/handle/10482/25724
https://dx.doi.org/10.1590/S0100-41582001000300007
dc.language.iso.fl_str_mv eng
language eng
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Sociedade Brasileira de Fitopatologia
publisher.none.fl_str_mv Sociedade Brasileira de Fitopatologia
dc.source.none.fl_str_mv reponame:Repositório Institucional da UnB
instname:Universidade de Brasília (UnB)
instacron:UNB
instname_str Universidade de Brasília (UnB)
instacron_str UNB
institution UNB
reponame_str Repositório Institucional da UnB
collection Repositório Institucional da UnB
repository.name.fl_str_mv Repositório Institucional da UnB - Universidade de Brasília (UnB)
repository.mail.fl_str_mv repositorio@unb.br
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