Cell-mediated immune response to Leishmania chagasi experimental infection of BALB/c immunosuppressed mice

Detalhes bibliográficos
Autor(a) principal: Machado,JG
Data de Publicação: 2010
Outros Autores: Hoffmann,JL, Krause,VLK, da Silva,AV, Dias-Melicio,LA, Langoni,H
Tipo de documento: Artigo
Idioma: eng
Título da fonte: The Journal of venomous animals and toxins including tropical diseases (Online)
Texto Completo: http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992010000100013
Resumo: Leishmaniasis, a zoonosis of worldwide distribution, presents a significant impact on immunosupressed patients. This study aimed to evaluate Leishmania chagasi infection in BALB/c mice immunosuppressed with dexamethasone. Spleen cells stimulated or not with L. chagasi were cultured for cytokine quantification (IFN-γ, IL-2, IL-4 and IL-10) by sandwich ELISA. Parasite loads in the spleen and liver were determined by means of culture microtitration. Immunosuppressed groups showed statistically lower spleen weight and CD4-cell percentage in blood on the day of infection and produced Th1 and Th2 cytokines on other days of the study. The other infected groups, weather immunosupressed or not, also produced Th1 and Th2 cytokines. Parasite loads in the spleen and liver were not statistically different among the groups. It was concluded that L. chagasi infection was not affected by dexamethasone-induced immunosuppression, probably due the reversible effect of the treatment.
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spelling Cell-mediated immune response to Leishmania chagasi experimental infection of BALB/c immunosuppressed micevisceral leishmaniasisimmunosuppressionimmunopathologymiceinterleukinsLeishmaniasis, a zoonosis of worldwide distribution, presents a significant impact on immunosupressed patients. This study aimed to evaluate Leishmania chagasi infection in BALB/c mice immunosuppressed with dexamethasone. Spleen cells stimulated or not with L. chagasi were cultured for cytokine quantification (IFN-γ, IL-2, IL-4 and IL-10) by sandwich ELISA. Parasite loads in the spleen and liver were determined by means of culture microtitration. Immunosuppressed groups showed statistically lower spleen weight and CD4-cell percentage in blood on the day of infection and produced Th1 and Th2 cytokines on other days of the study. The other infected groups, weather immunosupressed or not, also produced Th1 and Th2 cytokines. Parasite loads in the spleen and liver were not statistically different among the groups. It was concluded that L. chagasi infection was not affected by dexamethasone-induced immunosuppression, probably due the reversible effect of the treatment.Centro de Estudos de Venenos e Animais Peçonhentos (CEVAP/UNESP)2010-01-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersiontext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992010000100013Journal of Venomous Animals and Toxins including Tropical Diseases v.16 n.1 2010reponame:The Journal of venomous animals and toxins including tropical diseases (Online)instname:Universidade Estadual Paulista (UNESP)instacron:UNESP10.1590/S1678-91992010000100013info:eu-repo/semantics/openAccessMachado,JGHoffmann,JLKrause,VLKda Silva,AVDias-Melicio,LALangoni,Heng2010-03-18T00:00:00Zoai:scielo:S1678-91992010000100013Revistahttp://www.scielo.br/jvatitdPUBhttps://old.scielo.br/oai/scielo-oai.php||editorial@jvat.org.br1678-91991678-9180opendoar:2010-03-18T00:00The Journal of venomous animals and toxins including tropical diseases (Online) - Universidade Estadual Paulista (UNESP)false
dc.title.none.fl_str_mv Cell-mediated immune response to Leishmania chagasi experimental infection of BALB/c immunosuppressed mice
title Cell-mediated immune response to Leishmania chagasi experimental infection of BALB/c immunosuppressed mice
spellingShingle Cell-mediated immune response to Leishmania chagasi experimental infection of BALB/c immunosuppressed mice
Machado,JG
visceral leishmaniasis
immunosuppression
immunopathology
mice
interleukins
title_short Cell-mediated immune response to Leishmania chagasi experimental infection of BALB/c immunosuppressed mice
title_full Cell-mediated immune response to Leishmania chagasi experimental infection of BALB/c immunosuppressed mice
title_fullStr Cell-mediated immune response to Leishmania chagasi experimental infection of BALB/c immunosuppressed mice
title_full_unstemmed Cell-mediated immune response to Leishmania chagasi experimental infection of BALB/c immunosuppressed mice
title_sort Cell-mediated immune response to Leishmania chagasi experimental infection of BALB/c immunosuppressed mice
author Machado,JG
author_facet Machado,JG
Hoffmann,JL
Krause,VLK
da Silva,AV
Dias-Melicio,LA
Langoni,H
author_role author
author2 Hoffmann,JL
Krause,VLK
da Silva,AV
Dias-Melicio,LA
Langoni,H
author2_role author
author
author
author
author
dc.contributor.author.fl_str_mv Machado,JG
Hoffmann,JL
Krause,VLK
da Silva,AV
Dias-Melicio,LA
Langoni,H
dc.subject.por.fl_str_mv visceral leishmaniasis
immunosuppression
immunopathology
mice
interleukins
topic visceral leishmaniasis
immunosuppression
immunopathology
mice
interleukins
description Leishmaniasis, a zoonosis of worldwide distribution, presents a significant impact on immunosupressed patients. This study aimed to evaluate Leishmania chagasi infection in BALB/c mice immunosuppressed with dexamethasone. Spleen cells stimulated or not with L. chagasi were cultured for cytokine quantification (IFN-γ, IL-2, IL-4 and IL-10) by sandwich ELISA. Parasite loads in the spleen and liver were determined by means of culture microtitration. Immunosuppressed groups showed statistically lower spleen weight and CD4-cell percentage in blood on the day of infection and produced Th1 and Th2 cytokines on other days of the study. The other infected groups, weather immunosupressed or not, also produced Th1 and Th2 cytokines. Parasite loads in the spleen and liver were not statistically different among the groups. It was concluded that L. chagasi infection was not affected by dexamethasone-induced immunosuppression, probably due the reversible effect of the treatment.
publishDate 2010
dc.date.none.fl_str_mv 2010-01-01
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992010000100013
url http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992010000100013
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv 10.1590/S1678-91992010000100013
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv text/html
dc.publisher.none.fl_str_mv Centro de Estudos de Venenos e Animais Peçonhentos (CEVAP/UNESP)
publisher.none.fl_str_mv Centro de Estudos de Venenos e Animais Peçonhentos (CEVAP/UNESP)
dc.source.none.fl_str_mv Journal of Venomous Animals and Toxins including Tropical Diseases v.16 n.1 2010
reponame:The Journal of venomous animals and toxins including tropical diseases (Online)
instname:Universidade Estadual Paulista (UNESP)
instacron:UNESP
instname_str Universidade Estadual Paulista (UNESP)
instacron_str UNESP
institution UNESP
reponame_str The Journal of venomous animals and toxins including tropical diseases (Online)
collection The Journal of venomous animals and toxins including tropical diseases (Online)
repository.name.fl_str_mv The Journal of venomous animals and toxins including tropical diseases (Online) - Universidade Estadual Paulista (UNESP)
repository.mail.fl_str_mv ||editorial@jvat.org.br
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