Evaluation of the use of resistant starch in the prevention of colitis caused by the antibiotic tetracycline
Autor(a) principal: | |
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Data de Publicação: | 2022 |
Outros Autores: | , , , , , , |
Tipo de documento: | Artigo |
Idioma: | por |
Título da fonte: | Research, Society and Development |
Texto Completo: | https://rsdjournal.org/index.php/rsd/article/view/34080 |
Resumo: | This study aimed to evaluate the effect of diets with resistant starches in preventing the occurrence and/or severity of pseudomembranous colitis in animals treated with and without the antibiotic tetracycline. The experiment was carried out with 20 female Wistar rats, divided into four groups that received different diets: pregelatinized starch with 9.5% resistant starch; pregelatinized starch with 9.5% resistant starch and tetracycline; pregelatinized starch and resistant starch with 18.1% resistant starch and pregelatinized starch and resistant starch with 18.1% resistant starch and tetracycline. Weight, feed and water consumption were monitored for 21 days and analysis of total cholesterol, triglycerides and liver, muscle and cardiac glycogen were performed. Histological analysis of staining by hematoxylin-eosin and periodic acid Schiff of the animals' large intestine was performed. The results showed changes in the cytoarchitecture of the intestinal colon of the animals, which varied according to the treatment to which they were submitted, showing from mucosal atrophy in animals that received tetracycline, the formation of edema and congestion of vessels in the submucosa in animals fed 18 .1% of resistant starch and the presence of cellular debris in the lumen of the organ and loss of brush border when it was associated with tetracycline, demonstrating changes in the absorption and intestinal functioning of the animals, which contributes to the loss of organ homeostasis. |
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Evaluation of the use of resistant starch in the prevention of colitis caused by the antibiotic tetracyclineEvaluación del uso de almidón resistente en la prevención de la colitis causada por el antibiótico tetraciclinaAvaliação do uso de amido resistente na prevenção de colite provocada pelo antibiótico tetraciclinaResistant starchExperimental dietsHistological analysisTriglyceridesGlycogenCholesterol.Almidón resistenteDietas experimentalesAnálisis histológicoTriglicéridosGlucógenoColesterol.Amido resistenteDietas experimentaisAnálise histológicaTriglicerídeosGlicogênioColesterol.This study aimed to evaluate the effect of diets with resistant starches in preventing the occurrence and/or severity of pseudomembranous colitis in animals treated with and without the antibiotic tetracycline. The experiment was carried out with 20 female Wistar rats, divided into four groups that received different diets: pregelatinized starch with 9.5% resistant starch; pregelatinized starch with 9.5% resistant starch and tetracycline; pregelatinized starch and resistant starch with 18.1% resistant starch and pregelatinized starch and resistant starch with 18.1% resistant starch and tetracycline. Weight, feed and water consumption were monitored for 21 days and analysis of total cholesterol, triglycerides and liver, muscle and cardiac glycogen were performed. Histological analysis of staining by hematoxylin-eosin and periodic acid Schiff of the animals' large intestine was performed. The results showed changes in the cytoarchitecture of the intestinal colon of the animals, which varied according to the treatment to which they were submitted, showing from mucosal atrophy in animals that received tetracycline, the formation of edema and congestion of vessels in the submucosa in animals fed 18 .1% of resistant starch and the presence of cellular debris in the lumen of the organ and loss of brush border when it was associated with tetracycline, demonstrating changes in the absorption and intestinal functioning of the animals, which contributes to the loss of organ homeostasis.Este estudio tuvo como objetivo evaluar el efecto de las dietas con almidones resistentes en la prevención de la aparición y/o gravedad de la colitis pseudomembranosa en animales tratados con y sin el antibiótico tetraciclina. El experimento se realizó con 20 ratas Wistar hembra, divididas en cuatro grupos que recibieron diferentes dietas: almidón pregelatinizado con 9,5% de almidón resistente; almidón pregelatinizado con 9,5% de almidón resistente y tetraciclina; almidón pregelatinizado y almidón resistente con 18,1% de almidón resistente y almidón pregelatinizado y almidón resistente con 18,1% de almidón resistente y tetraciclina. Se controló el peso, el consumo de alimento y agua durante 21 días y se realizaron análisis de colesterol total, triglicéridos y glucógeno hepático, muscular y cardíaco. Se realizó el análisis histológico de la tinción con hematoxilina-eosina y ácido peryódico de Schiff del intestino grueso de los animales. Los resultados mostraron cambios en la citoarquitectura del colon intestinal de los animales, la cual varió de acuerdo al tratamiento al que fueron sometidos, mostrándose desde atrofia mucosa en animales que recibieron tetraciclina, formación de edema y congestión de vasos en la submucosa en animales alimentado con 18,1% de almidón resistente y la presencia de restos celulares en la luz del órgano y pérdida del borde en cepillo cuando se asoció con tetraciclina, demostrando cambios en la absorción y funcionamiento intestinal de los animales, lo que contribuye a la pérdida de homeostasis de órganos.Este trabalho teve como objetivo avaliar o efeito de dietas com amidos resistentes na prevenção da ocorrência e/ou gravidade da colite pseudomembranosa de animais tratados com e sem o antibiótico tetraciclina. O experimento foi realizado com 20 ratos wistar fêmeas, divididos em quatro grupos que receberam diferentes dietas: amido pré-gelatinizado com 9,5% de amido resistente; amido pré-gelatinizado com 9,5% de amido resistente e tetraciclina; amido pré-gelatinizado e amido resistente com 18,1% de amido resistente e amido pré-gelatinizado e amido resistente com 18,1% de amido resistente e tetraciclina. O peso, consumo de ração e água foram monitorados durante 21 dias e realizado análise de colesterol total, triglicerídeos e glicogênio hepático, muscular e cardíaco. Foi realizado análise histológica de coloração pela hematoxilina-eosina e pelo ácido periódico de Schiff do intestino grosso dos animais. Os resultados demonstraram alterações na citoarquitetura do colo intestinal dos animais, que variaram de acordo com o tratamento a que foram submetidos, mostrando desde atrofia da mucosa nos animais que receberam tetraciclina, a formação de edema e congestionamento de vasos na submucosa nos animais alimentados com 18,1% de amido resistente e a presença de debris celulares na luz do órgão e perda da bordadura em escova quando este foi associado a tetraciclina, demonstrando modificações na absorção e no funcionamento intestinal dos animais, o que contribui para a perda de homeostasia do órgão.Research, Society and Development2022-08-30info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionapplication/pdfhttps://rsdjournal.org/index.php/rsd/article/view/3408010.33448/rsd-v11i11.34080Research, Society and Development; Vol. 11 No. 11; e494111134080Research, Society and Development; Vol. 11 Núm. 11; e494111134080Research, Society and Development; v. 11 n. 11; e4941111340802525-3409reponame:Research, Society and Developmentinstname:Universidade Federal de Itajubá (UNIFEI)instacron:UNIFEIporhttps://rsdjournal.org/index.php/rsd/article/view/34080/28640Copyright (c) 2022 Armindo Antonio Alves; Bruna Lago Tagliapietra; Grasiela Dias de Campos Severi-Aguiar; Mariana Woord Mello; Débora Regina Delgado; Acácio Antônio Pigoso; Yoon Kil Chang; Maria Teresa Pedrosa Silva Clericihttps://creativecommons.org/licenses/by/4.0info:eu-repo/semantics/openAccessAlves, Armindo Antonio Tagliapietra, Bruna LagoSeveri-Aguiar, Grasiela Dias de Campos Mello, Mariana Woord Delgado, Débora Regina Pigoso, Acácio Antônio Chang, Yoon Kil Clerici, Maria Teresa Pedrosa Silva 2022-09-05T13:24:46Zoai:ojs.pkp.sfu.ca:article/34080Revistahttps://rsdjournal.org/index.php/rsd/indexPUBhttps://rsdjournal.org/index.php/rsd/oairsd.articles@gmail.com2525-34092525-3409opendoar:2024-01-17T09:49:31.547205Research, Society and Development - Universidade Federal de Itajubá (UNIFEI)false |
dc.title.none.fl_str_mv |
Evaluation of the use of resistant starch in the prevention of colitis caused by the antibiotic tetracycline Evaluación del uso de almidón resistente en la prevención de la colitis causada por el antibiótico tetraciclina Avaliação do uso de amido resistente na prevenção de colite provocada pelo antibiótico tetraciclina |
title |
Evaluation of the use of resistant starch in the prevention of colitis caused by the antibiotic tetracycline |
spellingShingle |
Evaluation of the use of resistant starch in the prevention of colitis caused by the antibiotic tetracycline Alves, Armindo Antonio Resistant starch Experimental diets Histological analysis Triglycerides Glycogen Cholesterol. Almidón resistente Dietas experimentales Análisis histológico Triglicéridos Glucógeno Colesterol. Amido resistente Dietas experimentais Análise histológica Triglicerídeos Glicogênio Colesterol. |
title_short |
Evaluation of the use of resistant starch in the prevention of colitis caused by the antibiotic tetracycline |
title_full |
Evaluation of the use of resistant starch in the prevention of colitis caused by the antibiotic tetracycline |
title_fullStr |
Evaluation of the use of resistant starch in the prevention of colitis caused by the antibiotic tetracycline |
title_full_unstemmed |
Evaluation of the use of resistant starch in the prevention of colitis caused by the antibiotic tetracycline |
title_sort |
Evaluation of the use of resistant starch in the prevention of colitis caused by the antibiotic tetracycline |
author |
Alves, Armindo Antonio |
author_facet |
Alves, Armindo Antonio Tagliapietra, Bruna Lago Severi-Aguiar, Grasiela Dias de Campos Mello, Mariana Woord Delgado, Débora Regina Pigoso, Acácio Antônio Chang, Yoon Kil Clerici, Maria Teresa Pedrosa Silva |
author_role |
author |
author2 |
Tagliapietra, Bruna Lago Severi-Aguiar, Grasiela Dias de Campos Mello, Mariana Woord Delgado, Débora Regina Pigoso, Acácio Antônio Chang, Yoon Kil Clerici, Maria Teresa Pedrosa Silva |
author2_role |
author author author author author author author |
dc.contributor.author.fl_str_mv |
Alves, Armindo Antonio Tagliapietra, Bruna Lago Severi-Aguiar, Grasiela Dias de Campos Mello, Mariana Woord Delgado, Débora Regina Pigoso, Acácio Antônio Chang, Yoon Kil Clerici, Maria Teresa Pedrosa Silva |
dc.subject.por.fl_str_mv |
Resistant starch Experimental diets Histological analysis Triglycerides Glycogen Cholesterol. Almidón resistente Dietas experimentales Análisis histológico Triglicéridos Glucógeno Colesterol. Amido resistente Dietas experimentais Análise histológica Triglicerídeos Glicogênio Colesterol. |
topic |
Resistant starch Experimental diets Histological analysis Triglycerides Glycogen Cholesterol. Almidón resistente Dietas experimentales Análisis histológico Triglicéridos Glucógeno Colesterol. Amido resistente Dietas experimentais Análise histológica Triglicerídeos Glicogênio Colesterol. |
description |
This study aimed to evaluate the effect of diets with resistant starches in preventing the occurrence and/or severity of pseudomembranous colitis in animals treated with and without the antibiotic tetracycline. The experiment was carried out with 20 female Wistar rats, divided into four groups that received different diets: pregelatinized starch with 9.5% resistant starch; pregelatinized starch with 9.5% resistant starch and tetracycline; pregelatinized starch and resistant starch with 18.1% resistant starch and pregelatinized starch and resistant starch with 18.1% resistant starch and tetracycline. Weight, feed and water consumption were monitored for 21 days and analysis of total cholesterol, triglycerides and liver, muscle and cardiac glycogen were performed. Histological analysis of staining by hematoxylin-eosin and periodic acid Schiff of the animals' large intestine was performed. The results showed changes in the cytoarchitecture of the intestinal colon of the animals, which varied according to the treatment to which they were submitted, showing from mucosal atrophy in animals that received tetracycline, the formation of edema and congestion of vessels in the submucosa in animals fed 18 .1% of resistant starch and the presence of cellular debris in the lumen of the organ and loss of brush border when it was associated with tetracycline, demonstrating changes in the absorption and intestinal functioning of the animals, which contributes to the loss of organ homeostasis. |
publishDate |
2022 |
dc.date.none.fl_str_mv |
2022-08-30 |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
https://rsdjournal.org/index.php/rsd/article/view/34080 10.33448/rsd-v11i11.34080 |
url |
https://rsdjournal.org/index.php/rsd/article/view/34080 |
identifier_str_mv |
10.33448/rsd-v11i11.34080 |
dc.language.iso.fl_str_mv |
por |
language |
por |
dc.relation.none.fl_str_mv |
https://rsdjournal.org/index.php/rsd/article/view/34080/28640 |
dc.rights.driver.fl_str_mv |
https://creativecommons.org/licenses/by/4.0 info:eu-repo/semantics/openAccess |
rights_invalid_str_mv |
https://creativecommons.org/licenses/by/4.0 |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
application/pdf |
dc.publisher.none.fl_str_mv |
Research, Society and Development |
publisher.none.fl_str_mv |
Research, Society and Development |
dc.source.none.fl_str_mv |
Research, Society and Development; Vol. 11 No. 11; e494111134080 Research, Society and Development; Vol. 11 Núm. 11; e494111134080 Research, Society and Development; v. 11 n. 11; e494111134080 2525-3409 reponame:Research, Society and Development instname:Universidade Federal de Itajubá (UNIFEI) instacron:UNIFEI |
instname_str |
Universidade Federal de Itajubá (UNIFEI) |
instacron_str |
UNIFEI |
institution |
UNIFEI |
reponame_str |
Research, Society and Development |
collection |
Research, Society and Development |
repository.name.fl_str_mv |
Research, Society and Development - Universidade Federal de Itajubá (UNIFEI) |
repository.mail.fl_str_mv |
rsd.articles@gmail.com |
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1797052722216697856 |