Assessment of the fatty acid composition of different parts of zebrafish fed diets incorporated with linseed and sunflower oils
Autor(a) principal: | |
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Data de Publicação: | 2021 |
Outros Autores: | , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Research, Society and Development |
Texto Completo: | https://rsdjournal.org/index.php/rsd/article/view/23177 |
Resumo: | This study aimed to evaluate the fatty acid composition of zebrafish fed diets containing linseed oil compared to sunflower oil. First, diets supplemented with linseed and sunflower were formulated, fish were fed for 40 days, and their parts collected for analysis. Diet composition analysis, extraction and derivatization of fatty acids, gas chromatography analysis, RNA extraction and cDNA synthesis, quantitative real-time polymerase chain reaction (qRT-PCR, and statistical analyses were performed. Linseed oil exhibited an omega-3 rich lipid profile. 18:3n-3 content incorporated into the muscle tissue of fish fed linseed oil was 50% higher than that fed sunflower oil. This higher amount of 18:3n-3 favored the production of 20:5n-3 and 22:6n-3 fatty acids by synthetic pathways in the organism since these fatty acids were not initially found in the oil composition. Furthermore, in all analyzed parts of zebrafish that were fed linseed oil, concentration of 20:4n-6 were lower, while 20:5n-3 and 22:6n-3 were higher compared to the same parts fed with sunflower oil. PCR expression assay showed no significant difference, indicating that linseed oil diet was not harmful. Thus, this work evidenced that synthesis of essential fatty acids, primarily omega-3 fatty acids, was greater in zebrafish upon consumption of diets supplemented with linseed oil. |
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Assessment of the fatty acid composition of different parts of zebrafish fed diets incorporated with linseed and sunflower oils Evaluación de la composición lipídica de diferentes partes del pez cebra alimentado con dietas incorporadas con aceites de linaza y girassolAvaliação da composição lipídica de diferentes partes de peixe-zebra alimentados com dietas incorporadas com óleos de linhaça e girassol Ração de peixesÁcido graxoAnálise lipídicaCromatografia gasosaPCR.Dieta de pescadoÁcidos grasosAnálisis de lipídiosCromatografía de gasesPCR.Fish dietFatty acidLipid analysisGas chromatographyPCR.This study aimed to evaluate the fatty acid composition of zebrafish fed diets containing linseed oil compared to sunflower oil. First, diets supplemented with linseed and sunflower were formulated, fish were fed for 40 days, and their parts collected for analysis. Diet composition analysis, extraction and derivatization of fatty acids, gas chromatography analysis, RNA extraction and cDNA synthesis, quantitative real-time polymerase chain reaction (qRT-PCR, and statistical analyses were performed. Linseed oil exhibited an omega-3 rich lipid profile. 18:3n-3 content incorporated into the muscle tissue of fish fed linseed oil was 50% higher than that fed sunflower oil. This higher amount of 18:3n-3 favored the production of 20:5n-3 and 22:6n-3 fatty acids by synthetic pathways in the organism since these fatty acids were not initially found in the oil composition. Furthermore, in all analyzed parts of zebrafish that were fed linseed oil, concentration of 20:4n-6 were lower, while 20:5n-3 and 22:6n-3 were higher compared to the same parts fed with sunflower oil. PCR expression assay showed no significant difference, indicating that linseed oil diet was not harmful. Thus, this work evidenced that synthesis of essential fatty acids, primarily omega-3 fatty acids, was greater in zebrafish upon consumption of diets supplemented with linseed oil. El objetivo de este estudio era evaluar la composición de ácidos grasos del pez cebra alimentado con dietas que contenían aceite de linaza en comparación con el aceite de girasol. En primer lugar, se formularon dietas suplementadas con linaza y girasol, se alimentó a los peces durante 40 días y se recogieron sus partes para su análisis. Se realizó un análisis de la composición de la dieta, la extracción y derivatización de los ácidos grasos, el análisis por cromatografía de gases, la extracción de ARN y la síntesis de ADNc, la reacción en cadena de la polimerasa en tiempo real (qRT-PCR) y los análisis estadísticos. El aceite de linaza mostró un perfil lipídico rico en omega-3. El contenido de 18:3n-3 incorporado en el tejido muscular de los peces alimentados con aceite de linaza fue un 50% superior al de los alimentados con aceite de girasol. Esta mayor cantidad de 18:3n-3 favoreció la producción de ácidos grasos 20:5n-3 y 22:6n-3 por vías sintéticas en el organismo, ya que estos ácidos grasos no se encontraban inicialmente en la composición del aceite. Además, en todas las partes analizadas del pez cebra que fue alimentado con aceite de linaza, la concentración de 20:4n-6 fue menor, mientras que la de 20:5n-3 y 22:6n-3 fue mayor en comparación con las mismas partes alimentadas con aceite de girasol. El ensayo de expresión por PCR no mostró diferencias significativas, lo que indica que la dieta con aceite de linaza no era perjudicial. Así pues, este trabajo evidenció que la síntesis de ácidos grasos esenciales, principalmente de ácidos grasos omega-3, fue mayor en el pez cebra al consumir dietas suplementadas con aceite de linaza. Este trabalho teve como objetivo avaliar a composição em ácidos graxos de peixes-zebra alimentados com dietas contendo óleo de linhaça em comparação com o óleo de girassol. Primeiramente, as dietas suplementadas com óleo de linhaça e girassol foram formuladas, os peixes receberam a alimentação durante 40 dias e suas partes foram coletadas para análise. Realizou-se análises de composição das dietas, extração e derivatização de ácidos graxos, análise por cromatografia gasosa, extração de RNA e síntese de cDNA, reação em cadeia da polimerase quantitativa em tempo real (qRT-PCR) e análise estatística. Após análises, o óleo de linhaça apresentou perfil lipídico rico, principalmente em n-3. A quantidade de ácido graxo 18:3n-3 incorporada ao tecido muscular do peixe que recebeu óleo de linhaça foi 50% superior ao alimentado com óleo de girassol. Essa maior concentração de 18:3n-3 favoreceu a produção de ácidos graxos 20:5n-3 e 22:6n-3 por vias sintéticas do organismo, uma vez que estes não foram encontrados inicialmente na composição dos óleos. Além disso, em todas as partes analisadas dos peixes-zebra que receberam ração de óleo de linhaça, as concentrações de 20:4n-6 foram inferiores, enquanto 20:5n-3 e 22:6n-3 foram superiores em comparação aos alimentos com ração de óleo de girassol. O ensaio de expressão PCR não apresentou diferença significativa, indicando que a ração com óleo de linhaça não era prejudicial. Desta forma, o trabalho evidenciou que a síntese de ácidos graxos essenciais, principalmente dos ácidos graxos n-3, é melhor estabelecida com o consumo de dieta adicionada de óleo de linhaça para peixe-zebra.Research, Society and Development2021-12-08info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionapplication/pdfhttps://rsdjournal.org/index.php/rsd/article/view/2317710.33448/rsd-v10i16.23177Research, Society and Development; Vol. 10 No. 16; e113101623177Research, Society and Development; Vol. 10 Núm. 16; e113101623177Research, Society and Development; v. 10 n. 16; e1131016231772525-3409reponame:Research, Society and Developmentinstname:Universidade Federal de Itajubá (UNIFEI)instacron:UNIFEIenghttps://rsdjournal.org/index.php/rsd/article/view/23177/20777Copyright (c) 2021 Victor Hugo Maldonado da Cruz; Geovane Aparecido Ramos da Silva; Matheus Campos Castro; Isadora Boaventura Ponhozi; Patrícia Magalhães de Souza; Jesui Vergilio Visentainer; Oscar Oliveira Santos Júniorhttps://creativecommons.org/licenses/by/4.0info:eu-repo/semantics/openAccessCruz, Victor Hugo Maldonado da Silva, Geovane Aparecido Ramos da Castro, Matheus Campos Ponhozi, Isadora Boaventura Souza, Patrícia Magalhães de Visentainer, Jesui Vergilio Santos Júnior, Oscar Oliveira 2021-12-20T11:03:07Zoai:ojs.pkp.sfu.ca:article/23177Revistahttps://rsdjournal.org/index.php/rsd/indexPUBhttps://rsdjournal.org/index.php/rsd/oairsd.articles@gmail.com2525-34092525-3409opendoar:2024-01-17T09:42:07.519683Research, Society and Development - Universidade Federal de Itajubá (UNIFEI)false |
dc.title.none.fl_str_mv |
Assessment of the fatty acid composition of different parts of zebrafish fed diets incorporated with linseed and sunflower oils Evaluación de la composición lipídica de diferentes partes del pez cebra alimentado con dietas incorporadas con aceites de linaza y girassol Avaliação da composição lipídica de diferentes partes de peixe-zebra alimentados com dietas incorporadas com óleos de linhaça e girassol |
title |
Assessment of the fatty acid composition of different parts of zebrafish fed diets incorporated with linseed and sunflower oils |
spellingShingle |
Assessment of the fatty acid composition of different parts of zebrafish fed diets incorporated with linseed and sunflower oils Cruz, Victor Hugo Maldonado da Ração de peixes Ácido graxo Análise lipídica Cromatografia gasosa PCR. Dieta de pescado Ácidos grasos Análisis de lipídios Cromatografía de gases PCR. Fish diet Fatty acid Lipid analysis Gas chromatography PCR. |
title_short |
Assessment of the fatty acid composition of different parts of zebrafish fed diets incorporated with linseed and sunflower oils |
title_full |
Assessment of the fatty acid composition of different parts of zebrafish fed diets incorporated with linseed and sunflower oils |
title_fullStr |
Assessment of the fatty acid composition of different parts of zebrafish fed diets incorporated with linseed and sunflower oils |
title_full_unstemmed |
Assessment of the fatty acid composition of different parts of zebrafish fed diets incorporated with linseed and sunflower oils |
title_sort |
Assessment of the fatty acid composition of different parts of zebrafish fed diets incorporated with linseed and sunflower oils |
author |
Cruz, Victor Hugo Maldonado da |
author_facet |
Cruz, Victor Hugo Maldonado da Silva, Geovane Aparecido Ramos da Castro, Matheus Campos Ponhozi, Isadora Boaventura Souza, Patrícia Magalhães de Visentainer, Jesui Vergilio Santos Júnior, Oscar Oliveira |
author_role |
author |
author2 |
Silva, Geovane Aparecido Ramos da Castro, Matheus Campos Ponhozi, Isadora Boaventura Souza, Patrícia Magalhães de Visentainer, Jesui Vergilio Santos Júnior, Oscar Oliveira |
author2_role |
author author author author author author |
dc.contributor.author.fl_str_mv |
Cruz, Victor Hugo Maldonado da Silva, Geovane Aparecido Ramos da Castro, Matheus Campos Ponhozi, Isadora Boaventura Souza, Patrícia Magalhães de Visentainer, Jesui Vergilio Santos Júnior, Oscar Oliveira |
dc.subject.por.fl_str_mv |
Ração de peixes Ácido graxo Análise lipídica Cromatografia gasosa PCR. Dieta de pescado Ácidos grasos Análisis de lipídios Cromatografía de gases PCR. Fish diet Fatty acid Lipid analysis Gas chromatography PCR. |
topic |
Ração de peixes Ácido graxo Análise lipídica Cromatografia gasosa PCR. Dieta de pescado Ácidos grasos Análisis de lipídios Cromatografía de gases PCR. Fish diet Fatty acid Lipid analysis Gas chromatography PCR. |
description |
This study aimed to evaluate the fatty acid composition of zebrafish fed diets containing linseed oil compared to sunflower oil. First, diets supplemented with linseed and sunflower were formulated, fish were fed for 40 days, and their parts collected for analysis. Diet composition analysis, extraction and derivatization of fatty acids, gas chromatography analysis, RNA extraction and cDNA synthesis, quantitative real-time polymerase chain reaction (qRT-PCR, and statistical analyses were performed. Linseed oil exhibited an omega-3 rich lipid profile. 18:3n-3 content incorporated into the muscle tissue of fish fed linseed oil was 50% higher than that fed sunflower oil. This higher amount of 18:3n-3 favored the production of 20:5n-3 and 22:6n-3 fatty acids by synthetic pathways in the organism since these fatty acids were not initially found in the oil composition. Furthermore, in all analyzed parts of zebrafish that were fed linseed oil, concentration of 20:4n-6 were lower, while 20:5n-3 and 22:6n-3 were higher compared to the same parts fed with sunflower oil. PCR expression assay showed no significant difference, indicating that linseed oil diet was not harmful. Thus, this work evidenced that synthesis of essential fatty acids, primarily omega-3 fatty acids, was greater in zebrafish upon consumption of diets supplemented with linseed oil. |
publishDate |
2021 |
dc.date.none.fl_str_mv |
2021-12-08 |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
https://rsdjournal.org/index.php/rsd/article/view/23177 10.33448/rsd-v10i16.23177 |
url |
https://rsdjournal.org/index.php/rsd/article/view/23177 |
identifier_str_mv |
10.33448/rsd-v10i16.23177 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
https://rsdjournal.org/index.php/rsd/article/view/23177/20777 |
dc.rights.driver.fl_str_mv |
https://creativecommons.org/licenses/by/4.0 info:eu-repo/semantics/openAccess |
rights_invalid_str_mv |
https://creativecommons.org/licenses/by/4.0 |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
application/pdf |
dc.publisher.none.fl_str_mv |
Research, Society and Development |
publisher.none.fl_str_mv |
Research, Society and Development |
dc.source.none.fl_str_mv |
Research, Society and Development; Vol. 10 No. 16; e113101623177 Research, Society and Development; Vol. 10 Núm. 16; e113101623177 Research, Society and Development; v. 10 n. 16; e113101623177 2525-3409 reponame:Research, Society and Development instname:Universidade Federal de Itajubá (UNIFEI) instacron:UNIFEI |
instname_str |
Universidade Federal de Itajubá (UNIFEI) |
instacron_str |
UNIFEI |
institution |
UNIFEI |
reponame_str |
Research, Society and Development |
collection |
Research, Society and Development |
repository.name.fl_str_mv |
Research, Society and Development - Universidade Federal de Itajubá (UNIFEI) |
repository.mail.fl_str_mv |
rsd.articles@gmail.com |
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1797052759267082240 |