Alternative and sustainable method for performing Gram and Wirtz-Conklin bacterial staining: Environmental and economic relevance in practical microbiology teaching

Detalhes bibliográficos
Autor(a) principal: Reine, Fabricio Ueda
Data de Publicação: 2021
Outros Autores: Souza, Lorena Aparecida Bianchi de, Menezes, Jéssica Lima de, Gomes, Rosana Ferreira Carli Cangussu, Souza, Aline Campos Reis de, Mota, Camila Alves, Abreu Filho, Benício Alves de
Tipo de documento: Artigo
Idioma: por
Título da fonte: Research, Society and Development
Texto Completo: https://rsdjournal.org/index.php/rsd/article/view/17585
Resumo: Stained preparations are the most commonly microscopic exam used in microbiology, as they provide a better view of microorganisms. Bearing in mind that Gram and Wirtz-Conklin staining techniques are commonly used in the routine of microbiology laboratories and in practical classes, this project aimed to compare the standard staining technique on slide surfaces, with the technique by immersion, which in turn can avoid waste of dyes, environmental impact and reduce laboratory and teaching costs. Thus, bacterial smears (Escherichia coli; Staphylococcus aureus; Alicyclobacillus acidoterrestris and Bacillus subtilis) were made on glass slides, followed by flame fixation. After, the slides were submitted to staining using the surface technique and the immersion technique. Subsequently, the slides were viewed under a standard optical microscope and the resolution of the images and tinting characteristics were evaluated and recorded. The staining by immersion, both for Gram stain and Wirtz-Conklin, avoided the waste of the dyes used in the techniques, as well as, the saturation of the solutions after many slide passes. Thus, the immersion staining technique is favorable for use in classes or microbiology laboratories. The application of this technique has shown significant results for the reduction of waste and, consequently, the impacts on the environment and the economy of educational institutions.
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spelling Alternative and sustainable method for performing Gram and Wirtz-Conklin bacterial staining: Environmental and economic relevance in practical microbiology teachingMétodo alternativo y sostenible para realizar la tinción bacteriana Gram y Wirtz-Conklin: Relevancia ambiental y económica en la enseñanza práctica de microbiologiaMétodo alternativo e sustentável para a realização de coloração bacteriana de Gram e Wirtz-Conklin: Relevância ambiental e econômica no ensino prático da microbiologia Coloração de GramColoração de Wirtz-ConklinMicrorganismosMicrobiologiaEscherichia coliStaphylococcus aureusAlicyclobacillus acidoterrestrisBacillus subtilis.Tinción de GramTinción de Wirtz-ConklinMicroorganismosMicrobiologíaEscherichia coliStaphylococcus aureusAlicyclobacillus acidoterrestrisBacillus subtilis.Gram’s stainWirtz-Conklin stainMicroorganismsMicrobiologyEscherichia coliStaphylococcus aureusAlicyclobacillus acidoterrestrisBacillus subtilis.Stained preparations are the most commonly microscopic exam used in microbiology, as they provide a better view of microorganisms. Bearing in mind that Gram and Wirtz-Conklin staining techniques are commonly used in the routine of microbiology laboratories and in practical classes, this project aimed to compare the standard staining technique on slide surfaces, with the technique by immersion, which in turn can avoid waste of dyes, environmental impact and reduce laboratory and teaching costs. Thus, bacterial smears (Escherichia coli; Staphylococcus aureus; Alicyclobacillus acidoterrestris and Bacillus subtilis) were made on glass slides, followed by flame fixation. After, the slides were submitted to staining using the surface technique and the immersion technique. Subsequently, the slides were viewed under a standard optical microscope and the resolution of the images and tinting characteristics were evaluated and recorded. The staining by immersion, both for Gram stain and Wirtz-Conklin, avoided the waste of the dyes used in the techniques, as well as, the saturation of the solutions after many slide passes. Thus, the immersion staining technique is favorable for use in classes or microbiology laboratories. The application of this technique has shown significant results for the reduction of waste and, consequently, the impacts on the environment and the economy of educational institutions.Las preparaciones teñidas son los exámenes microscópicos más utilizados en microbiología, ya que proporcionan una mejor visión de los microorganismos. Teniendo en cuenta que las técnicas de tinción de Gram y Wirtz-Conklin se utilizan comúnmente en la rutina de los laboratorios de microbiología y en las clases de microscopía teñida, este proyecto tuvo como objetivo comparar la técnica de tinción estándar en superficies de portaobjetos, con la técnica de inmersión, que a su vez puede evitar el desperdicio de tintes, el impacto ambiental y reducir los costos de laboratorio y enseñanza. Por lo tanto, se hicieron frotis bacterianos (Escherichia coli; Staphylococcus aureus; Alicyclobacillus acidoterrestris y Bacillus subtilis) en portaobjetos, seguidos de fijación con llama. Después de este procedimiento, los portaobjetos se sometieron a tinción mediante la técnica de superficie y la técnica de inmersión. Posteriormente, los portaobjetos se visualizaron bajo un microscopio óptico estándar y se evaluó y registró la resolución de las imágenes y las características de tinción. La técnica de tinción por inmersión, tanto para la tinción de Gram como para Wirtz-Conklin, evitó el desperdicio de los colorantes utilizados en las técnicas, así como la saturación de las soluciones tras muchas pasadas de portaobjetos. Por tanto, la técnica de tinción por inmersión es favorable para su uso en clases o en laboratorios de microbiología. La aplicación de esta técnica ha mostrado resultados significativos para la reducción de residuos y, en consecuencia, los impactos sobre el medio ambiente y la economía de las instituciones educativas.As preparações coradas são os exames microscópicos mais comumente empregados em microbiologia, pois proporcionam uma melhor visualização dos microrganismos. Tendo em vista que as técnicas de coloração de Gram e Wirtz-Conklin são comumente utilizadas na rotina de laboratórios de microbiologia e nas aulas de microscopias coradas, este projeto teve como objetivo comparar a técnica padrão de coloração em superfície das lâminas, com a técnica por imersão, que por sua vez pode evitar o desperdício de corantes, impacto ambiental e diminuir custos laboratoriais e no ensino. Dessa forma, foram feitos esfregaços bacterianos (Escherichia coli; Staphylococcus aureus; Alicyclobacillus acidoterrestris e Bacillus subtilis) em lâmina, seguidos de fixação em chama. Após esse procedimento as lâminas foram submetidas à coloração pela técnica de superfície e pela técnica de imersão. Posteriormente as lâminas foram visualizadas em microscópio óptico comum e a resolução das imagens e características tintoriais foram avaliadas e registradas. A técnica de coloração por imersão, tanto para a coloração de Gram quanto para Wirtz-Conklin, evitou desperdício dos corantes utilizados nas técnicas, bem como, a saturação das soluções após muitas passagens de lâminas. Assim, a técnica de coloração por imersão é favorável para o uso nas aulas ou nos laboratórios de microbiologia. A aplicação dessa técnica mostrou resultados significativos para a diminuição do desperdício e, consequentemente, os impactos ao meio ambiente e à economia das instituições de ensino.Research, Society and Development2021-07-22info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionapplication/pdfhttps://rsdjournal.org/index.php/rsd/article/view/1758510.33448/rsd-v10i9.17585Research, Society and Development; Vol. 10 No. 9; e9510917585Research, Society and Development; Vol. 10 Núm. 9; e9510917585Research, Society and Development; v. 10 n. 9; e95109175852525-3409reponame:Research, Society and Developmentinstname:Universidade Federal de Itajubá (UNIFEI)instacron:UNIFEIporhttps://rsdjournal.org/index.php/rsd/article/view/17585/16034Copyright (c) 2021 Fabricio Ueda Reine; Lorena Aparecida Bianchi de Souza; Jéssica Lima de Menezes; Rosana Ferreira Carli Cangussu Gomes; Aline Campos Reis de Souza; Camila Alves Mota; Benício Alves de Abreu Filhohttps://creativecommons.org/licenses/by/4.0info:eu-repo/semantics/openAccessReine, Fabricio UedaSouza, Lorena Aparecida Bianchi de Menezes, Jéssica Lima de Gomes, Rosana Ferreira Carli Cangussu Souza, Aline Campos Reis de Mota, Camila Alves Abreu Filho, Benício Alves de2021-09-12T14:28:06Zoai:ojs.pkp.sfu.ca:article/17585Revistahttps://rsdjournal.org/index.php/rsd/indexPUBhttps://rsdjournal.org/index.php/rsd/oairsd.articles@gmail.com2525-34092525-3409opendoar:2024-01-17T09:37:54.087360Research, Society and Development - Universidade Federal de Itajubá (UNIFEI)false
dc.title.none.fl_str_mv Alternative and sustainable method for performing Gram and Wirtz-Conklin bacterial staining: Environmental and economic relevance in practical microbiology teaching
Método alternativo y sostenible para realizar la tinción bacteriana Gram y Wirtz-Conklin: Relevancia ambiental y económica en la enseñanza práctica de microbiologia
Método alternativo e sustentável para a realização de coloração bacteriana de Gram e Wirtz-Conklin: Relevância ambiental e econômica no ensino prático da microbiologia
title Alternative and sustainable method for performing Gram and Wirtz-Conklin bacterial staining: Environmental and economic relevance in practical microbiology teaching
spellingShingle Alternative and sustainable method for performing Gram and Wirtz-Conklin bacterial staining: Environmental and economic relevance in practical microbiology teaching
Reine, Fabricio Ueda
Coloração de Gram
Coloração de Wirtz-Conklin
Microrganismos
Microbiologia
Escherichia coli
Staphylococcus aureus
Alicyclobacillus acidoterrestris
Bacillus subtilis.
Tinción de Gram
Tinción de Wirtz-Conklin
Microorganismos
Microbiología
Escherichia coli
Staphylococcus aureus
Alicyclobacillus acidoterrestris
Bacillus subtilis.
Gram’s stain
Wirtz-Conklin stain
Microorganisms
Microbiology
Escherichia coli
Staphylococcus aureus
Alicyclobacillus acidoterrestris
Bacillus subtilis.
title_short Alternative and sustainable method for performing Gram and Wirtz-Conklin bacterial staining: Environmental and economic relevance in practical microbiology teaching
title_full Alternative and sustainable method for performing Gram and Wirtz-Conklin bacterial staining: Environmental and economic relevance in practical microbiology teaching
title_fullStr Alternative and sustainable method for performing Gram and Wirtz-Conklin bacterial staining: Environmental and economic relevance in practical microbiology teaching
title_full_unstemmed Alternative and sustainable method for performing Gram and Wirtz-Conklin bacterial staining: Environmental and economic relevance in practical microbiology teaching
title_sort Alternative and sustainable method for performing Gram and Wirtz-Conklin bacterial staining: Environmental and economic relevance in practical microbiology teaching
author Reine, Fabricio Ueda
author_facet Reine, Fabricio Ueda
Souza, Lorena Aparecida Bianchi de
Menezes, Jéssica Lima de
Gomes, Rosana Ferreira Carli Cangussu
Souza, Aline Campos Reis de
Mota, Camila Alves
Abreu Filho, Benício Alves de
author_role author
author2 Souza, Lorena Aparecida Bianchi de
Menezes, Jéssica Lima de
Gomes, Rosana Ferreira Carli Cangussu
Souza, Aline Campos Reis de
Mota, Camila Alves
Abreu Filho, Benício Alves de
author2_role author
author
author
author
author
author
dc.contributor.author.fl_str_mv Reine, Fabricio Ueda
Souza, Lorena Aparecida Bianchi de
Menezes, Jéssica Lima de
Gomes, Rosana Ferreira Carli Cangussu
Souza, Aline Campos Reis de
Mota, Camila Alves
Abreu Filho, Benício Alves de
dc.subject.por.fl_str_mv Coloração de Gram
Coloração de Wirtz-Conklin
Microrganismos
Microbiologia
Escherichia coli
Staphylococcus aureus
Alicyclobacillus acidoterrestris
Bacillus subtilis.
Tinción de Gram
Tinción de Wirtz-Conklin
Microorganismos
Microbiología
Escherichia coli
Staphylococcus aureus
Alicyclobacillus acidoterrestris
Bacillus subtilis.
Gram’s stain
Wirtz-Conklin stain
Microorganisms
Microbiology
Escherichia coli
Staphylococcus aureus
Alicyclobacillus acidoterrestris
Bacillus subtilis.
topic Coloração de Gram
Coloração de Wirtz-Conklin
Microrganismos
Microbiologia
Escherichia coli
Staphylococcus aureus
Alicyclobacillus acidoterrestris
Bacillus subtilis.
Tinción de Gram
Tinción de Wirtz-Conklin
Microorganismos
Microbiología
Escherichia coli
Staphylococcus aureus
Alicyclobacillus acidoterrestris
Bacillus subtilis.
Gram’s stain
Wirtz-Conklin stain
Microorganisms
Microbiology
Escherichia coli
Staphylococcus aureus
Alicyclobacillus acidoterrestris
Bacillus subtilis.
description Stained preparations are the most commonly microscopic exam used in microbiology, as they provide a better view of microorganisms. Bearing in mind that Gram and Wirtz-Conklin staining techniques are commonly used in the routine of microbiology laboratories and in practical classes, this project aimed to compare the standard staining technique on slide surfaces, with the technique by immersion, which in turn can avoid waste of dyes, environmental impact and reduce laboratory and teaching costs. Thus, bacterial smears (Escherichia coli; Staphylococcus aureus; Alicyclobacillus acidoterrestris and Bacillus subtilis) were made on glass slides, followed by flame fixation. After, the slides were submitted to staining using the surface technique and the immersion technique. Subsequently, the slides were viewed under a standard optical microscope and the resolution of the images and tinting characteristics were evaluated and recorded. The staining by immersion, both for Gram stain and Wirtz-Conklin, avoided the waste of the dyes used in the techniques, as well as, the saturation of the solutions after many slide passes. Thus, the immersion staining technique is favorable for use in classes or microbiology laboratories. The application of this technique has shown significant results for the reduction of waste and, consequently, the impacts on the environment and the economy of educational institutions.
publishDate 2021
dc.date.none.fl_str_mv 2021-07-22
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv https://rsdjournal.org/index.php/rsd/article/view/17585
10.33448/rsd-v10i9.17585
url https://rsdjournal.org/index.php/rsd/article/view/17585
identifier_str_mv 10.33448/rsd-v10i9.17585
dc.language.iso.fl_str_mv por
language por
dc.relation.none.fl_str_mv https://rsdjournal.org/index.php/rsd/article/view/17585/16034
dc.rights.driver.fl_str_mv https://creativecommons.org/licenses/by/4.0
info:eu-repo/semantics/openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by/4.0
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Research, Society and Development
publisher.none.fl_str_mv Research, Society and Development
dc.source.none.fl_str_mv Research, Society and Development; Vol. 10 No. 9; e9510917585
Research, Society and Development; Vol. 10 Núm. 9; e9510917585
Research, Society and Development; v. 10 n. 9; e9510917585
2525-3409
reponame:Research, Society and Development
instname:Universidade Federal de Itajubá (UNIFEI)
instacron:UNIFEI
instname_str Universidade Federal de Itajubá (UNIFEI)
instacron_str UNIFEI
institution UNIFEI
reponame_str Research, Society and Development
collection Research, Society and Development
repository.name.fl_str_mv Research, Society and Development - Universidade Federal de Itajubá (UNIFEI)
repository.mail.fl_str_mv rsd.articles@gmail.com
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