Proteus mirabilis carrying NTEKPC-IId, blaNDM-1, blaOXA-10, aph(3')-VI, qnrD1 and IncQ and Col3M plasmids from a hospital in Recife-PE, Brazil

Detalhes bibliográficos
Autor(a) principal: Beltrão, Elizabeth Maria Bispo
Data de Publicação: 2021
Outros Autores: Oliveira, Érica Maria, Vasconcelos, Crhisllane Rafaele dos Santos Vasconcelos, Rezende, Antônio Mauro, Lopes, Ana Catarina de Souza
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Research, Society and Development
Texto Completo: https://rsdjournal.org/index.php/rsd/article/view/22919
Resumo: The present study objective to characterize the clinical aspects of a patient infected with two strains of P. mirabilis and the presence of resistance determinants in the two isolates from a patient at a public hospital in Recife-PE, Brazil. The total DNA of the isolates was extracted and submitted to PCR and amplicon sequencing for the investigation of resistance genes, blaKPC, blaOXA-10, blaOXA-23, blaOXA-48, blaOXA-58, blaVIM, blaIMP, blaSPM, blaGES, blaNDM, qnrD and aac(6')-Ib). Isolate P21-A2 harbored the aac(6')-Ib, blaOXA-10 and qnrD genes. One of the isolates, P20-A2, was selected for plasmid DNA sequencing. The results showed that the patient developed multiple infections with various pathogens including two strains of P. mirabilis. The patient was hospitalized for 103 days, had septic shock of skin, abdominal, pulmonary and ulcer focus, and died. Isolate P20-A2 harbored the genes blaNDM, qnrD, aph(3')-VI, blaKPC and blaOXA-10, and plasmids IncQ and Col3M, together with NTEKPC-IId. To our knowledge, this is the first report of P. mirabilis harboring NTEKPC-IId. Although P. mirabilis is standing out as a cause of nosocomial infections and a resistant multidrug pathogen, this species is still neglected, the emergence of these P. mirabilis isolates harboring aforementioned resistance determinants and the plasmids IncQ and Col3M demonstrate the potential for dissemination of important resistance genes, mainly in the case of P. mirabilis.
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spelling Proteus mirabilis carrying NTEKPC-IId, blaNDM-1, blaOXA-10, aph(3')-VI, qnrD1 and IncQ and Col3M plasmids from a hospital in Recife-PE, BrazilProteus mirabilis transportando NTEKPC-IId, blaNDM-1, blaOXA-10, aph(3')-VI, qnrD1 e plásmidos IncQ y Col3M de un hospital en Recife-PE, BrasilProteus mirabilis portador de NTEKPC-IId, blaNDM-1, blaOXA-10, aph(3’)-VI, qnrD1 e plasmídeos IncQ e Col3M proveniente de paciente internado em hospital de Recife-PE, BrasilProteus mirabilisNTEKPC-IIdIncQCol3M.Proteus mirabilisNTEKPC-IIdIncQCol3M.Proteus mirabilisNTEKPC-IIdIncQCol3M.The present study objective to characterize the clinical aspects of a patient infected with two strains of P. mirabilis and the presence of resistance determinants in the two isolates from a patient at a public hospital in Recife-PE, Brazil. The total DNA of the isolates was extracted and submitted to PCR and amplicon sequencing for the investigation of resistance genes, blaKPC, blaOXA-10, blaOXA-23, blaOXA-48, blaOXA-58, blaVIM, blaIMP, blaSPM, blaGES, blaNDM, qnrD and aac(6')-Ib). Isolate P21-A2 harbored the aac(6')-Ib, blaOXA-10 and qnrD genes. One of the isolates, P20-A2, was selected for plasmid DNA sequencing. The results showed that the patient developed multiple infections with various pathogens including two strains of P. mirabilis. The patient was hospitalized for 103 days, had septic shock of skin, abdominal, pulmonary and ulcer focus, and died. Isolate P20-A2 harbored the genes blaNDM, qnrD, aph(3')-VI, blaKPC and blaOXA-10, and plasmids IncQ and Col3M, together with NTEKPC-IId. To our knowledge, this is the first report of P. mirabilis harboring NTEKPC-IId. Although P. mirabilis is standing out as a cause of nosocomial infections and a resistant multidrug pathogen, this species is still neglected, the emergence of these P. mirabilis isolates harboring aforementioned resistance determinants and the plasmids IncQ and Col3M demonstrate the potential for dissemination of important resistance genes, mainly in the case of P. mirabilis.El presente estudio tuvo como objetivo caracterizar los aspectos clínicos de un paciente infectado con dos cepas de P. mirabilis y la presencia de determinantes de resistencia en los dos aislamientos de un paciente de un hospital público de Recife-PE, Brasil. El DNA total de los aislamientos fue extraído y sometido a PCR y secuenciación de amplicones para la investigación de genes de resistencia, blaKPC, blaOXA-10, blaOXA-23, blaOXA-48, blaOXA-58, blaVIM, blaIMP, blaSPM, blaGES, blaNDM, qnrD y aac(6')-Ib). El aislamiento P21-A2 albergaba los genes aac(6')-Ib, blaOXA-10 y qnrD. Uno de los aislados, P20-A2, se seleccionó para la secuenciación del DNA plasmídico. Los resultados mostraron que el paciente desarrolló múltiples infecciones con varios patógenos, incluidas dos cepas de P. mirabilis. El paciente estuvo hospitalizado durante 103 días, presentó shock séptico de piel, foco abdominal, pulmonar y ulceroso, y falleció. El aislamiento P20-A2 albergaba los genes blaNDM, qnrD, aph(3')-VI, blaKPC y blaOXA-10, y los plásmidos IncQ y Col3M, junto con NTEKPC-IId. Hasta donde sabemos, este es el primer informe de P. mirabilis que alberga NTEKPC-IId. Aunque P. mirabilis se destaca como una causa de infecciones nosocomiales y un patógeno resistente a múltiples fármacos, esta especie aún se descuida, la aparición de estos aislados de P. mirabilis que albergan los determinantes de resistencia antes mencionados y los plásmidos IncQ y Col3M demuestran el potencial de diseminación de importantes genes de resistencia, principalmente en el caso de P. mirabilis.O presente estudo teve por objetivo caracterizar aspectos clínicos de um paciente com infecção por duas cepas de P. mirabilis e a presença de determinantes de resistência nos dois isolados provenientes de um paciente de um hospital público de Recife-PE, Brasil. O DNA total dos isolados foi extraído e submetido a PCR e sequenciamento dos amplicons para a investigação de genes de resistência, blaKPC, blaOXA-10, blaOXA-23, blaOXA-48, blaOXA-58, blaVIM, blaIMP, blaSPM, blaGES, blaNDM, qnrD e aac(6’)-Ib). O isolado P21-A2 albergava os genes aac(6’)-Ib, blaOXA-10 e qnrD. Um dos isolados, P20-A2, foi selecionado para o sequenciamento do DNA plasmidial. Os resultados mostraram que o paciente desenvolveu diversas infecções por vários patógenos incluindo duas cepas de P. mirabilis. O paciente ficou internado por 103 dias, teve choque séptico de foco cutâneo, abdominal, pulmonar e úlcera, e veio a óbito. O isolado P20-A2 albergava os genes blaNDM, qnrD, aph(3’)-VI, blaKPC e blaOXA-10, e os plasmídeos IncQ e Col3M, juntamente com NTEKPC-IId. Para o nosso conhecimento, este é o primeiro relato de P. mirabilis albergando NTEKPC-IId. Apesar de P. mirabilis estar se destacando como causador de infecções nosocomiais e patógeno multidroga resistente, esta espécie ainda é negligência,  a emergência desses isolados de P. mirabilis albergando determinantes de resistência antes mencionados e os plasmídeos IncQ e Col3M demonstra o potencial de disseminação de importantes genes de resistência, principalmente se tratando de P. mirabilis.Research, Society and Development2021-11-28info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionapplication/pdfhttps://rsdjournal.org/index.php/rsd/article/view/2291910.33448/rsd-v10i15.22919Research, Society and Development; Vol. 10 No. 15; e399101522919Research, Society and Development; Vol. 10 Núm. 15; e399101522919Research, Society and Development; v. 10 n. 15; e3991015229192525-3409reponame:Research, Society and Developmentinstname:Universidade Federal de Itajubá (UNIFEI)instacron:UNIFEIenghttps://rsdjournal.org/index.php/rsd/article/view/22919/20423Copyright (c) 2021 Elizabeth Maria Bispo Beltrão; Érica Maria Oliveira; Crhisllane Rafaele dos Santos Vasconcelos Vasconcelos; Antônio Mauro Rezende; Ana Catarina de Souza Lopeshttps://creativecommons.org/licenses/by/4.0info:eu-repo/semantics/openAccessBeltrão, Elizabeth Maria BispoOliveira, Érica MariaVasconcelos, Crhisllane Rafaele dos Santos VasconcelosRezende, Antônio Mauro Lopes, Ana Catarina de Souza2021-12-06T10:13:53Zoai:ojs.pkp.sfu.ca:article/22919Revistahttps://rsdjournal.org/index.php/rsd/indexPUBhttps://rsdjournal.org/index.php/rsd/oairsd.articles@gmail.com2525-34092525-3409opendoar:2024-01-17T09:41:56.740388Research, Society and Development - Universidade Federal de Itajubá (UNIFEI)false
dc.title.none.fl_str_mv Proteus mirabilis carrying NTEKPC-IId, blaNDM-1, blaOXA-10, aph(3')-VI, qnrD1 and IncQ and Col3M plasmids from a hospital in Recife-PE, Brazil
Proteus mirabilis transportando NTEKPC-IId, blaNDM-1, blaOXA-10, aph(3')-VI, qnrD1 e plásmidos IncQ y Col3M de un hospital en Recife-PE, Brasil
Proteus mirabilis portador de NTEKPC-IId, blaNDM-1, blaOXA-10, aph(3’)-VI, qnrD1 e plasmídeos IncQ e Col3M proveniente de paciente internado em hospital de Recife-PE, Brasil
title Proteus mirabilis carrying NTEKPC-IId, blaNDM-1, blaOXA-10, aph(3')-VI, qnrD1 and IncQ and Col3M plasmids from a hospital in Recife-PE, Brazil
spellingShingle Proteus mirabilis carrying NTEKPC-IId, blaNDM-1, blaOXA-10, aph(3')-VI, qnrD1 and IncQ and Col3M plasmids from a hospital in Recife-PE, Brazil
Beltrão, Elizabeth Maria Bispo
Proteus mirabilis
NTEKPC-IId
IncQ
Col3M.
Proteus mirabilis
NTEKPC-IId
IncQ
Col3M.
Proteus mirabilis
NTEKPC-IId
IncQ
Col3M.
title_short Proteus mirabilis carrying NTEKPC-IId, blaNDM-1, blaOXA-10, aph(3')-VI, qnrD1 and IncQ and Col3M plasmids from a hospital in Recife-PE, Brazil
title_full Proteus mirabilis carrying NTEKPC-IId, blaNDM-1, blaOXA-10, aph(3')-VI, qnrD1 and IncQ and Col3M plasmids from a hospital in Recife-PE, Brazil
title_fullStr Proteus mirabilis carrying NTEKPC-IId, blaNDM-1, blaOXA-10, aph(3')-VI, qnrD1 and IncQ and Col3M plasmids from a hospital in Recife-PE, Brazil
title_full_unstemmed Proteus mirabilis carrying NTEKPC-IId, blaNDM-1, blaOXA-10, aph(3')-VI, qnrD1 and IncQ and Col3M plasmids from a hospital in Recife-PE, Brazil
title_sort Proteus mirabilis carrying NTEKPC-IId, blaNDM-1, blaOXA-10, aph(3')-VI, qnrD1 and IncQ and Col3M plasmids from a hospital in Recife-PE, Brazil
author Beltrão, Elizabeth Maria Bispo
author_facet Beltrão, Elizabeth Maria Bispo
Oliveira, Érica Maria
Vasconcelos, Crhisllane Rafaele dos Santos Vasconcelos
Rezende, Antônio Mauro
Lopes, Ana Catarina de Souza
author_role author
author2 Oliveira, Érica Maria
Vasconcelos, Crhisllane Rafaele dos Santos Vasconcelos
Rezende, Antônio Mauro
Lopes, Ana Catarina de Souza
author2_role author
author
author
author
dc.contributor.author.fl_str_mv Beltrão, Elizabeth Maria Bispo
Oliveira, Érica Maria
Vasconcelos, Crhisllane Rafaele dos Santos Vasconcelos
Rezende, Antônio Mauro
Lopes, Ana Catarina de Souza
dc.subject.por.fl_str_mv Proteus mirabilis
NTEKPC-IId
IncQ
Col3M.
Proteus mirabilis
NTEKPC-IId
IncQ
Col3M.
Proteus mirabilis
NTEKPC-IId
IncQ
Col3M.
topic Proteus mirabilis
NTEKPC-IId
IncQ
Col3M.
Proteus mirabilis
NTEKPC-IId
IncQ
Col3M.
Proteus mirabilis
NTEKPC-IId
IncQ
Col3M.
description The present study objective to characterize the clinical aspects of a patient infected with two strains of P. mirabilis and the presence of resistance determinants in the two isolates from a patient at a public hospital in Recife-PE, Brazil. The total DNA of the isolates was extracted and submitted to PCR and amplicon sequencing for the investigation of resistance genes, blaKPC, blaOXA-10, blaOXA-23, blaOXA-48, blaOXA-58, blaVIM, blaIMP, blaSPM, blaGES, blaNDM, qnrD and aac(6')-Ib). Isolate P21-A2 harbored the aac(6')-Ib, blaOXA-10 and qnrD genes. One of the isolates, P20-A2, was selected for plasmid DNA sequencing. The results showed that the patient developed multiple infections with various pathogens including two strains of P. mirabilis. The patient was hospitalized for 103 days, had septic shock of skin, abdominal, pulmonary and ulcer focus, and died. Isolate P20-A2 harbored the genes blaNDM, qnrD, aph(3')-VI, blaKPC and blaOXA-10, and plasmids IncQ and Col3M, together with NTEKPC-IId. To our knowledge, this is the first report of P. mirabilis harboring NTEKPC-IId. Although P. mirabilis is standing out as a cause of nosocomial infections and a resistant multidrug pathogen, this species is still neglected, the emergence of these P. mirabilis isolates harboring aforementioned resistance determinants and the plasmids IncQ and Col3M demonstrate the potential for dissemination of important resistance genes, mainly in the case of P. mirabilis.
publishDate 2021
dc.date.none.fl_str_mv 2021-11-28
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv https://rsdjournal.org/index.php/rsd/article/view/22919
10.33448/rsd-v10i15.22919
url https://rsdjournal.org/index.php/rsd/article/view/22919
identifier_str_mv 10.33448/rsd-v10i15.22919
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv https://rsdjournal.org/index.php/rsd/article/view/22919/20423
dc.rights.driver.fl_str_mv https://creativecommons.org/licenses/by/4.0
info:eu-repo/semantics/openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by/4.0
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Research, Society and Development
publisher.none.fl_str_mv Research, Society and Development
dc.source.none.fl_str_mv Research, Society and Development; Vol. 10 No. 15; e399101522919
Research, Society and Development; Vol. 10 Núm. 15; e399101522919
Research, Society and Development; v. 10 n. 15; e399101522919
2525-3409
reponame:Research, Society and Development
instname:Universidade Federal de Itajubá (UNIFEI)
instacron:UNIFEI
instname_str Universidade Federal de Itajubá (UNIFEI)
instacron_str UNIFEI
institution UNIFEI
reponame_str Research, Society and Development
collection Research, Society and Development
repository.name.fl_str_mv Research, Society and Development - Universidade Federal de Itajubá (UNIFEI)
repository.mail.fl_str_mv rsd.articles@gmail.com
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