Isolation, identification and characterization of a novel high level β-glucosidase-producing Lichtheimia ramosa strain

Detalhes bibliográficos
Autor(a) principal: Gonçalves, Fabiano Avelino
Data de Publicação: 2013
Outros Autores: Leite, Rodrigo Simões Ribeiro, Rodrigues, André [UNESP], Argandoña, Eliana Janet Sanjinez, Fonseca, Gustavo Graciano
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da UNESP
Texto Completo: http://dx.doi.org/10.1016/j.bcab.2013.06.006
http://hdl.handle.net/11449/227207
Resumo: The aim of this work was to prospect for microorganisms in the local environment, selecting those with higher production of cellulolytic and hemicellulolytic enzymes. A total of 36 microorganisms were isolated in a sugarcane-based medium. Colonies were replated in medium containing carboxymethylcellulose as the main carbon source, where all grew, confirming their potential for plant biomass degradation. The enzyme production was evaluated by cultivating the isolated microorganisms in solid-state bioprocess using two different carbon sources, sugarcane bagasse and wheat bran. Among the microorganisms obtained, isolate 11, identified as Lichtheimia ramosa, presented a high level β-glucosidase production of 15.58. U/mL, as well as 2.41. U/mL of xylanase and 2.13. U/mL of CMCase when cultivated on wheat bran. Changes in temperature and cultivation time favored a higher production of β-glucosidase: 17.26. U/mL in wheat bran-based medium at 35. °C for 120. h. © 2013 Elsevier Ltd.
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spelling Isolation, identification and characterization of a novel high level β-glucosidase-producing Lichtheimia ramosa strainScreeningSugarcane bagasseWheat branThe aim of this work was to prospect for microorganisms in the local environment, selecting those with higher production of cellulolytic and hemicellulolytic enzymes. A total of 36 microorganisms were isolated in a sugarcane-based medium. Colonies were replated in medium containing carboxymethylcellulose as the main carbon source, where all grew, confirming their potential for plant biomass degradation. The enzyme production was evaluated by cultivating the isolated microorganisms in solid-state bioprocess using two different carbon sources, sugarcane bagasse and wheat bran. Among the microorganisms obtained, isolate 11, identified as Lichtheimia ramosa, presented a high level β-glucosidase production of 15.58. U/mL, as well as 2.41. U/mL of xylanase and 2.13. U/mL of CMCase when cultivated on wheat bran. Changes in temperature and cultivation time favored a higher production of β-glucosidase: 17.26. U/mL in wheat bran-based medium at 35. °C for 120. h. © 2013 Elsevier Ltd.Laboratory of Bioengineering Faculty of Engineering Federal University of Grande Dourados, Dourados, Mato Grosso do SulLaboratory of Enzymology Faculty of Biological and Environmental Sciences Federal University of Grande Dourados, Dourados, Mato Grosso do SulLaboratory of Fungal Ecology and Systematics Department of Biochemistry and Microbiology São Paulo State University, Rio Claro, São PauloLaboratory of Food Technology Faculty of Engineering Federal University of Grande Dourados, Dourados, Mato Grosso do SulLaboratory of Fungal Ecology and Systematics Department of Biochemistry and Microbiology São Paulo State University, Rio Claro, São PauloFederal University of Grande DouradosUniversidade Estadual Paulista (UNESP)Gonçalves, Fabiano AvelinoLeite, Rodrigo Simões RibeiroRodrigues, André [UNESP]Argandoña, Eliana Janet SanjinezFonseca, Gustavo Graciano2022-04-29T07:12:07Z2022-04-29T07:12:07Z2013-10-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article377-384http://dx.doi.org/10.1016/j.bcab.2013.06.006Biocatalysis and Agricultural Biotechnology, v. 2, n. 4, p. 377-384, 2013.1878-8181http://hdl.handle.net/11449/22720710.1016/j.bcab.2013.06.0062-s2.0-84884245249Scopusreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengBiocatalysis and Agricultural Biotechnologyinfo:eu-repo/semantics/openAccess2022-04-29T07:12:07Zoai:repositorio.unesp.br:11449/227207Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462024-08-05T22:57:33.906007Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false
dc.title.none.fl_str_mv Isolation, identification and characterization of a novel high level β-glucosidase-producing Lichtheimia ramosa strain
title Isolation, identification and characterization of a novel high level β-glucosidase-producing Lichtheimia ramosa strain
spellingShingle Isolation, identification and characterization of a novel high level β-glucosidase-producing Lichtheimia ramosa strain
Gonçalves, Fabiano Avelino
Screening
Sugarcane bagasse
Wheat bran
title_short Isolation, identification and characterization of a novel high level β-glucosidase-producing Lichtheimia ramosa strain
title_full Isolation, identification and characterization of a novel high level β-glucosidase-producing Lichtheimia ramosa strain
title_fullStr Isolation, identification and characterization of a novel high level β-glucosidase-producing Lichtheimia ramosa strain
title_full_unstemmed Isolation, identification and characterization of a novel high level β-glucosidase-producing Lichtheimia ramosa strain
title_sort Isolation, identification and characterization of a novel high level β-glucosidase-producing Lichtheimia ramosa strain
author Gonçalves, Fabiano Avelino
author_facet Gonçalves, Fabiano Avelino
Leite, Rodrigo Simões Ribeiro
Rodrigues, André [UNESP]
Argandoña, Eliana Janet Sanjinez
Fonseca, Gustavo Graciano
author_role author
author2 Leite, Rodrigo Simões Ribeiro
Rodrigues, André [UNESP]
Argandoña, Eliana Janet Sanjinez
Fonseca, Gustavo Graciano
author2_role author
author
author
author
dc.contributor.none.fl_str_mv Federal University of Grande Dourados
Universidade Estadual Paulista (UNESP)
dc.contributor.author.fl_str_mv Gonçalves, Fabiano Avelino
Leite, Rodrigo Simões Ribeiro
Rodrigues, André [UNESP]
Argandoña, Eliana Janet Sanjinez
Fonseca, Gustavo Graciano
dc.subject.por.fl_str_mv Screening
Sugarcane bagasse
Wheat bran
topic Screening
Sugarcane bagasse
Wheat bran
description The aim of this work was to prospect for microorganisms in the local environment, selecting those with higher production of cellulolytic and hemicellulolytic enzymes. A total of 36 microorganisms were isolated in a sugarcane-based medium. Colonies were replated in medium containing carboxymethylcellulose as the main carbon source, where all grew, confirming their potential for plant biomass degradation. The enzyme production was evaluated by cultivating the isolated microorganisms in solid-state bioprocess using two different carbon sources, sugarcane bagasse and wheat bran. Among the microorganisms obtained, isolate 11, identified as Lichtheimia ramosa, presented a high level β-glucosidase production of 15.58. U/mL, as well as 2.41. U/mL of xylanase and 2.13. U/mL of CMCase when cultivated on wheat bran. Changes in temperature and cultivation time favored a higher production of β-glucosidase: 17.26. U/mL in wheat bran-based medium at 35. °C for 120. h. © 2013 Elsevier Ltd.
publishDate 2013
dc.date.none.fl_str_mv 2013-10-01
2022-04-29T07:12:07Z
2022-04-29T07:12:07Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://dx.doi.org/10.1016/j.bcab.2013.06.006
Biocatalysis and Agricultural Biotechnology, v. 2, n. 4, p. 377-384, 2013.
1878-8181
http://hdl.handle.net/11449/227207
10.1016/j.bcab.2013.06.006
2-s2.0-84884245249
url http://dx.doi.org/10.1016/j.bcab.2013.06.006
http://hdl.handle.net/11449/227207
identifier_str_mv Biocatalysis and Agricultural Biotechnology, v. 2, n. 4, p. 377-384, 2013.
1878-8181
10.1016/j.bcab.2013.06.006
2-s2.0-84884245249
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv Biocatalysis and Agricultural Biotechnology
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv 377-384
dc.source.none.fl_str_mv Scopus
reponame:Repositório Institucional da UNESP
instname:Universidade Estadual Paulista (UNESP)
instacron:UNESP
instname_str Universidade Estadual Paulista (UNESP)
instacron_str UNESP
institution UNESP
reponame_str Repositório Institucional da UNESP
collection Repositório Institucional da UNESP
repository.name.fl_str_mv Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)
repository.mail.fl_str_mv
_version_ 1808129477939560448

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