Separation of the toxic zierin from Zollernia ilicifolia by high speed countercurrent chromatography

Detalhes bibliográficos
Autor(a) principal: Coelho, R. G. [UNESP]
Data de Publicação: 2007
Outros Autores: Calvo, T. R. [UNESP], Santos, Lourdes Campaner dos [UNESP], Di Stasi, L. C. [UNESP], Vilegas, Wagner [UNESP]
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da UNESP
Texto Completo: http://www.ibb.unesp.br/Home/Departamentos/Botanica/RBPM-RevistaBrasileiradePlantasMedicinais/artigo7_v9_n4.pdf
http://hdl.handle.net/11449/69965
Resumo: Preliminary pharmacological assays of the 70% methanol extract from the leaves of the Brazilian medicinal plant Zollernia ilicifolia Vog. (Fabaceae) showed analgesic and antiulcerogenic effects. Previous analyses have shown that this extract contains, besides flavonoid glycosides and saponins, a toxic cyanogenic glycoside. Flavonoids and saponins are compounds reported in literature with antiulcerogenic activity. In this work, we developed a methodology to separate the cyanogenic glycoside from these compounds in order to obtain enough amount of material to perform pharmacological assays. The cyanogenic glycoside zierin (2S)-β-D-glucopyranosyloxy-(3-hydroxy-phenyl)- acetonitrile was separated from the other components by high speed countercurrent chromatography (HSCCC). The solvent system used was composed of chloroform-methanol-n-propanol-water (5:6:1:4, v/v/v/v). This technique led to the separation of zierin from the possible active compounds of Zollernia ilicifolia.
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spelling Separation of the toxic zierin from Zollernia ilicifolia by high speed countercurrent chromatographyHigh-speed counter-current chromatographyZierinZollernia ilicifoliachloroformflavonoidglycosidemethanolplant extractpropanolsaponin derivativesolventunclassified drugwaterzierinzollernia ilicifolia extractchromatographydrug structurehigh performance liquid chromatographyhigh speed countercurrent chromatographylegumemedicinal plantseparation techniquethin layer chromatographyFabaceaeZollerniaPreliminary pharmacological assays of the 70% methanol extract from the leaves of the Brazilian medicinal plant Zollernia ilicifolia Vog. (Fabaceae) showed analgesic and antiulcerogenic effects. Previous analyses have shown that this extract contains, besides flavonoid glycosides and saponins, a toxic cyanogenic glycoside. Flavonoids and saponins are compounds reported in literature with antiulcerogenic activity. In this work, we developed a methodology to separate the cyanogenic glycoside from these compounds in order to obtain enough amount of material to perform pharmacological assays. The cyanogenic glycoside zierin (2S)-β-D-glucopyranosyloxy-(3-hydroxy-phenyl)- acetonitrile was separated from the other components by high speed countercurrent chromatography (HSCCC). The solvent system used was composed of chloroform-methanol-n-propanol-water (5:6:1:4, v/v/v/v). This technique led to the separation of zierin from the possible active compounds of Zollernia ilicifolia.Departamento de Química Orgânica Instituto de Química Universidade Estadual Paulista, CP 355, CEP 14801-970, Araraquara, SPDepartamento de Farmacologia Instituto de Biociências Universidade Estadual Paulista, 18618-000, Botucatu, SPDepartamento de Química Orgânica Instituto de Química Universidade Estadual Paulista, CP 355, CEP 14801-970, Araraquara, SPDepartamento de Farmacologia Instituto de Biociências Universidade Estadual Paulista, 18618-000, Botucatu, SPUniversidade Estadual Paulista (Unesp)Coelho, R. G. [UNESP]Calvo, T. R. [UNESP]Santos, Lourdes Campaner dos [UNESP]Di Stasi, L. C. [UNESP]Vilegas, Wagner [UNESP]2014-05-27T11:22:38Z2014-05-27T11:22:38Z2007-11-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article39-43application/pdfhttp://www.ibb.unesp.br/Home/Departamentos/Botanica/RBPM-RevistaBrasileiradePlantasMedicinais/artigo7_v9_n4.pdfRevista Brasileira de Plantas Medicinais, v. 9, n. 4, p. 39-43, 2007.1516-0572http://hdl.handle.net/11449/699652-s2.0-385491647862-s2.0-38549164786.pdf1697547325096457353825364060297735382536406029770000-0003-3032-2556Scopusreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengRevista Brasileira de Plantas Medicinais0,199info:eu-repo/semantics/openAccess2024-01-03T06:25:21Zoai:repositorio.unesp.br:11449/69965Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462024-01-03T06:25:21Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false
dc.title.none.fl_str_mv Separation of the toxic zierin from Zollernia ilicifolia by high speed countercurrent chromatography
title Separation of the toxic zierin from Zollernia ilicifolia by high speed countercurrent chromatography
spellingShingle Separation of the toxic zierin from Zollernia ilicifolia by high speed countercurrent chromatography
Coelho, R. G. [UNESP]
High-speed counter-current chromatography
Zierin
Zollernia ilicifolia
chloroform
flavonoid
glycoside
methanol
plant extract
propanol
saponin derivative
solvent
unclassified drug
water
zierin
zollernia ilicifolia extract
chromatography
drug structure
high performance liquid chromatography
high speed countercurrent chromatography
legume
medicinal plant
separation technique
thin layer chromatography
Fabaceae
Zollernia
title_short Separation of the toxic zierin from Zollernia ilicifolia by high speed countercurrent chromatography
title_full Separation of the toxic zierin from Zollernia ilicifolia by high speed countercurrent chromatography
title_fullStr Separation of the toxic zierin from Zollernia ilicifolia by high speed countercurrent chromatography
title_full_unstemmed Separation of the toxic zierin from Zollernia ilicifolia by high speed countercurrent chromatography
title_sort Separation of the toxic zierin from Zollernia ilicifolia by high speed countercurrent chromatography
author Coelho, R. G. [UNESP]
author_facet Coelho, R. G. [UNESP]
Calvo, T. R. [UNESP]
Santos, Lourdes Campaner dos [UNESP]
Di Stasi, L. C. [UNESP]
Vilegas, Wagner [UNESP]
author_role author
author2 Calvo, T. R. [UNESP]
Santos, Lourdes Campaner dos [UNESP]
Di Stasi, L. C. [UNESP]
Vilegas, Wagner [UNESP]
author2_role author
author
author
author
dc.contributor.none.fl_str_mv Universidade Estadual Paulista (Unesp)
dc.contributor.author.fl_str_mv Coelho, R. G. [UNESP]
Calvo, T. R. [UNESP]
Santos, Lourdes Campaner dos [UNESP]
Di Stasi, L. C. [UNESP]
Vilegas, Wagner [UNESP]
dc.subject.por.fl_str_mv High-speed counter-current chromatography
Zierin
Zollernia ilicifolia
chloroform
flavonoid
glycoside
methanol
plant extract
propanol
saponin derivative
solvent
unclassified drug
water
zierin
zollernia ilicifolia extract
chromatography
drug structure
high performance liquid chromatography
high speed countercurrent chromatography
legume
medicinal plant
separation technique
thin layer chromatography
Fabaceae
Zollernia
topic High-speed counter-current chromatography
Zierin
Zollernia ilicifolia
chloroform
flavonoid
glycoside
methanol
plant extract
propanol
saponin derivative
solvent
unclassified drug
water
zierin
zollernia ilicifolia extract
chromatography
drug structure
high performance liquid chromatography
high speed countercurrent chromatography
legume
medicinal plant
separation technique
thin layer chromatography
Fabaceae
Zollernia
description Preliminary pharmacological assays of the 70% methanol extract from the leaves of the Brazilian medicinal plant Zollernia ilicifolia Vog. (Fabaceae) showed analgesic and antiulcerogenic effects. Previous analyses have shown that this extract contains, besides flavonoid glycosides and saponins, a toxic cyanogenic glycoside. Flavonoids and saponins are compounds reported in literature with antiulcerogenic activity. In this work, we developed a methodology to separate the cyanogenic glycoside from these compounds in order to obtain enough amount of material to perform pharmacological assays. The cyanogenic glycoside zierin (2S)-β-D-glucopyranosyloxy-(3-hydroxy-phenyl)- acetonitrile was separated from the other components by high speed countercurrent chromatography (HSCCC). The solvent system used was composed of chloroform-methanol-n-propanol-water (5:6:1:4, v/v/v/v). This technique led to the separation of zierin from the possible active compounds of Zollernia ilicifolia.
publishDate 2007
dc.date.none.fl_str_mv 2007-11-01
2014-05-27T11:22:38Z
2014-05-27T11:22:38Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://www.ibb.unesp.br/Home/Departamentos/Botanica/RBPM-RevistaBrasileiradePlantasMedicinais/artigo7_v9_n4.pdf
Revista Brasileira de Plantas Medicinais, v. 9, n. 4, p. 39-43, 2007.
1516-0572
http://hdl.handle.net/11449/69965
2-s2.0-38549164786
2-s2.0-38549164786.pdf
1697547325096457
3538253640602977
3538253640602977
0000-0003-3032-2556
url http://www.ibb.unesp.br/Home/Departamentos/Botanica/RBPM-RevistaBrasileiradePlantasMedicinais/artigo7_v9_n4.pdf
http://hdl.handle.net/11449/69965
identifier_str_mv Revista Brasileira de Plantas Medicinais, v. 9, n. 4, p. 39-43, 2007.
1516-0572
2-s2.0-38549164786
2-s2.0-38549164786.pdf
1697547325096457
3538253640602977
0000-0003-3032-2556
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv Revista Brasileira de Plantas Medicinais
0,199
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv 39-43
application/pdf
dc.source.none.fl_str_mv Scopus
reponame:Repositório Institucional da UNESP
instname:Universidade Estadual Paulista (UNESP)
instacron:UNESP
instname_str Universidade Estadual Paulista (UNESP)
instacron_str UNESP
institution UNESP
reponame_str Repositório Institucional da UNESP
collection Repositório Institucional da UNESP
repository.name.fl_str_mv Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)
repository.mail.fl_str_mv
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