Chitosan in association with osteogenic factors as a cell-homing platform for dentin regeneration: Analysis in a pulp-in-a-chip model

Detalhes bibliográficos
Autor(a) principal: Bordini, E. A.F. [UNESP]
Data de Publicação: 2022
Outros Autores: Cassiano, F. B., Bronze-Uhle, E. S., Alamo, L., Hebling, J. [UNESP], de Souza Costa, C. A. [UNESP], Soares, D. G.
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da UNESP
Texto Completo: http://dx.doi.org/10.1016/j.dental.2022.02.004
http://hdl.handle.net/11449/230442
Resumo: Objective: In this paper we propose the association of β-glycerophosphate (βGP) and calcium-hydroxide with chitosan (CH) to formulate a porous bioactive scaffold suitable as a cell-homing platform for dentin regeneration. Methods: Calcium hydroxide and βGP solutions were incorporated into chitosan to modulate scaffold architecture and composition by a phase separation technique. Architecture, chemical composition, and degradability were evaluated, and biological characterizations were performed by the seeding of dental pulp cells (DPCs) onto scaffolds, or by cultivating them in contact with leachable components (extracts), to determine cytocompatibility and odontoblastic differentiation. Cell-free scaffolds were then positioned in intimate contact with a 3D culture of DPCs in a pulp-in-a-chip platform under simulated pulp pressure. Cell mobilization and odontoblastic marker expression were evaluated. Deposition of mineralized matrix was assessed in direct contact with dentin, in the absence of osteogenic factors. Results: Incorporation of calcium hydroxide and βGP generated a stable porous chitosan scaffold containing Ca-P nanoglobule topography (CH-Ca-βGP), which favored cell viability, alkaline phosphatase activity, and mineralized matrix deposition by cells seeded onto the scaffold structure and at a distance. The pulp-in-a-chip assay denoted its chemotactic and bioactive potential, since dentin sialoprotein-positive DPCs from 3D culture adhered to CH-Ca-βGP more than to plain chitosan. The higher deposition of mineralized matrix onto the scaffold and surrounding dentin was also observed. Significance: A CH-Ca-βGP scaffold creates a microenvironment capable of mobilizing DPC migration toward its structure, harnessing the odontogenic potential and culminating in the expression of a highly mineralizing phenotype, key factors for a cell-homing strategy.
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spelling Chitosan in association with osteogenic factors as a cell-homing platform for dentin regeneration: Analysis in a pulp-in-a-chip modelChitosanDental pulp cellsDentin regenerationβ-glycerophosphateObjective: In this paper we propose the association of β-glycerophosphate (βGP) and calcium-hydroxide with chitosan (CH) to formulate a porous bioactive scaffold suitable as a cell-homing platform for dentin regeneration. Methods: Calcium hydroxide and βGP solutions were incorporated into chitosan to modulate scaffold architecture and composition by a phase separation technique. Architecture, chemical composition, and degradability were evaluated, and biological characterizations were performed by the seeding of dental pulp cells (DPCs) onto scaffolds, or by cultivating them in contact with leachable components (extracts), to determine cytocompatibility and odontoblastic differentiation. Cell-free scaffolds were then positioned in intimate contact with a 3D culture of DPCs in a pulp-in-a-chip platform under simulated pulp pressure. Cell mobilization and odontoblastic marker expression were evaluated. Deposition of mineralized matrix was assessed in direct contact with dentin, in the absence of osteogenic factors. Results: Incorporation of calcium hydroxide and βGP generated a stable porous chitosan scaffold containing Ca-P nanoglobule topography (CH-Ca-βGP), which favored cell viability, alkaline phosphatase activity, and mineralized matrix deposition by cells seeded onto the scaffold structure and at a distance. The pulp-in-a-chip assay denoted its chemotactic and bioactive potential, since dentin sialoprotein-positive DPCs from 3D culture adhered to CH-Ca-βGP more than to plain chitosan. The higher deposition of mineralized matrix onto the scaffold and surrounding dentin was also observed. Significance: A CH-Ca-βGP scaffold creates a microenvironment capable of mobilizing DPC migration toward its structure, harnessing the odontogenic potential and culminating in the expression of a highly mineralizing phenotype, key factors for a cell-homing strategy.Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)Department of Physiology and Pathology Univ. Estadual Paulista – UNESP Araraquara School of Dentistry, Humaitá Street, 1680, SPDepartment of Operative Dentistry Endodontics and Dental Materials Sao Paulo University – USP Bauru School of Dentistry, Al. Dr. Octávio Pinheiro Brizola, 9-75, SPDepartment of Orthodontics and Pediatric Dentistry Univ. Estadual Paulista – UNESP Araraquara School of Dentistry, Humaitá Street, 1680, SPDepartment of Physiology and Pathology Univ. Estadual Paulista – UNESP Araraquara School of Dentistry, Humaitá Street, 1680, SPDepartment of Orthodontics and Pediatric Dentistry Univ. Estadual Paulista – UNESP Araraquara School of Dentistry, Humaitá Street, 1680, SPFAPESP: 2016/15674-5FAPESP: 2017/20181-0CNPq: 429934 2018 1Universidade Estadual Paulista (UNESP)Universidade de São Paulo (USP)Bordini, E. A.F. [UNESP]Cassiano, F. B.Bronze-Uhle, E. S.Alamo, L.Hebling, J. [UNESP]de Souza Costa, C. A. [UNESP]Soares, D. G.2022-04-29T08:39:57Z2022-04-29T08:39:57Z2022-01-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articlehttp://dx.doi.org/10.1016/j.dental.2022.02.004Dental Materials.0109-5641http://hdl.handle.net/11449/23044210.1016/j.dental.2022.02.0042-s2.0-85125117317Scopusreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengDental Materialsinfo:eu-repo/semantics/openAccess2024-09-27T14:04:57Zoai:repositorio.unesp.br:11449/230442Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestrepositoriounesp@unesp.bropendoar:29462024-09-27T14:04:57Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false
dc.title.none.fl_str_mv Chitosan in association with osteogenic factors as a cell-homing platform for dentin regeneration: Analysis in a pulp-in-a-chip model
title Chitosan in association with osteogenic factors as a cell-homing platform for dentin regeneration: Analysis in a pulp-in-a-chip model
spellingShingle Chitosan in association with osteogenic factors as a cell-homing platform for dentin regeneration: Analysis in a pulp-in-a-chip model
Bordini, E. A.F. [UNESP]
Chitosan
Dental pulp cells
Dentin regeneration
β-glycerophosphate
title_short Chitosan in association with osteogenic factors as a cell-homing platform for dentin regeneration: Analysis in a pulp-in-a-chip model
title_full Chitosan in association with osteogenic factors as a cell-homing platform for dentin regeneration: Analysis in a pulp-in-a-chip model
title_fullStr Chitosan in association with osteogenic factors as a cell-homing platform for dentin regeneration: Analysis in a pulp-in-a-chip model
title_full_unstemmed Chitosan in association with osteogenic factors as a cell-homing platform for dentin regeneration: Analysis in a pulp-in-a-chip model
title_sort Chitosan in association with osteogenic factors as a cell-homing platform for dentin regeneration: Analysis in a pulp-in-a-chip model
author Bordini, E. A.F. [UNESP]
author_facet Bordini, E. A.F. [UNESP]
Cassiano, F. B.
Bronze-Uhle, E. S.
Alamo, L.
Hebling, J. [UNESP]
de Souza Costa, C. A. [UNESP]
Soares, D. G.
author_role author
author2 Cassiano, F. B.
Bronze-Uhle, E. S.
Alamo, L.
Hebling, J. [UNESP]
de Souza Costa, C. A. [UNESP]
Soares, D. G.
author2_role author
author
author
author
author
author
dc.contributor.none.fl_str_mv Universidade Estadual Paulista (UNESP)
Universidade de São Paulo (USP)
dc.contributor.author.fl_str_mv Bordini, E. A.F. [UNESP]
Cassiano, F. B.
Bronze-Uhle, E. S.
Alamo, L.
Hebling, J. [UNESP]
de Souza Costa, C. A. [UNESP]
Soares, D. G.
dc.subject.por.fl_str_mv Chitosan
Dental pulp cells
Dentin regeneration
β-glycerophosphate
topic Chitosan
Dental pulp cells
Dentin regeneration
β-glycerophosphate
description Objective: In this paper we propose the association of β-glycerophosphate (βGP) and calcium-hydroxide with chitosan (CH) to formulate a porous bioactive scaffold suitable as a cell-homing platform for dentin regeneration. Methods: Calcium hydroxide and βGP solutions were incorporated into chitosan to modulate scaffold architecture and composition by a phase separation technique. Architecture, chemical composition, and degradability were evaluated, and biological characterizations were performed by the seeding of dental pulp cells (DPCs) onto scaffolds, or by cultivating them in contact with leachable components (extracts), to determine cytocompatibility and odontoblastic differentiation. Cell-free scaffolds were then positioned in intimate contact with a 3D culture of DPCs in a pulp-in-a-chip platform under simulated pulp pressure. Cell mobilization and odontoblastic marker expression were evaluated. Deposition of mineralized matrix was assessed in direct contact with dentin, in the absence of osteogenic factors. Results: Incorporation of calcium hydroxide and βGP generated a stable porous chitosan scaffold containing Ca-P nanoglobule topography (CH-Ca-βGP), which favored cell viability, alkaline phosphatase activity, and mineralized matrix deposition by cells seeded onto the scaffold structure and at a distance. The pulp-in-a-chip assay denoted its chemotactic and bioactive potential, since dentin sialoprotein-positive DPCs from 3D culture adhered to CH-Ca-βGP more than to plain chitosan. The higher deposition of mineralized matrix onto the scaffold and surrounding dentin was also observed. Significance: A CH-Ca-βGP scaffold creates a microenvironment capable of mobilizing DPC migration toward its structure, harnessing the odontogenic potential and culminating in the expression of a highly mineralizing phenotype, key factors for a cell-homing strategy.
publishDate 2022
dc.date.none.fl_str_mv 2022-04-29T08:39:57Z
2022-04-29T08:39:57Z
2022-01-01
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://dx.doi.org/10.1016/j.dental.2022.02.004
Dental Materials.
0109-5641
http://hdl.handle.net/11449/230442
10.1016/j.dental.2022.02.004
2-s2.0-85125117317
url http://dx.doi.org/10.1016/j.dental.2022.02.004
http://hdl.handle.net/11449/230442
identifier_str_mv Dental Materials.
0109-5641
10.1016/j.dental.2022.02.004
2-s2.0-85125117317
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv Dental Materials
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.source.none.fl_str_mv Scopus
reponame:Repositório Institucional da UNESP
instname:Universidade Estadual Paulista (UNESP)
instacron:UNESP
instname_str Universidade Estadual Paulista (UNESP)
instacron_str UNESP
institution UNESP
reponame_str Repositório Institucional da UNESP
collection Repositório Institucional da UNESP
repository.name.fl_str_mv Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)
repository.mail.fl_str_mv repositoriounesp@unesp.br
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