Pathogenicity and genetic profile of oral Porphyromonas species from canine periodontitis
Autor(a) principal: | |
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Data de Publicação: | 2017 |
Outros Autores: | , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UNESP |
Texto Completo: | http://dx.doi.org/10.1016/j.archoralbio.2017.07.001 http://hdl.handle.net/11449/174858 |
Resumo: | Objective In this study, the presence of the prtC and fimA genes involved in the pathogenicity of oral Porphyromonas spp. isolated from dogs with periodontitis and healthy, as well as their genetic diversity was investigated. Design Thirty-two Beagle dogs, 24 with periodontitis and 8 healthy were evaluated. Subgingival samples from only one gingival site of both groups were collected. Bacteria grown in anaerobiosis were identified by RAPID ID 32A kits. From each strain the respective DNA was obtained and used to genotyping by conventional PCR and AP-PCR. Results Dogs with periodontitis harbored 28 P. gulae, 2 P. creviocaricanis, 1 P. cangingivalis and 7 P. macacae; and from healthy dogs, 11 P. gulae and 5 P. circumdentaria. In P. gulae isolated from periodontal dogs the gene prtC was observed in 19 (67.85%) and in 7 (63.63%) from healthy dogs. P. gulae strains from periodontal dogs harbored either the gene fimA I or fimA II; while strains from healthy dogs harbored the gene fimA I, fimA II, fimA III or fimA IV, as well as 1 P. circumdentaria the gene fimA II. By AP-PCR strains were grouped in different clusters suggesting heterogeneity of these microorganisms. Conclusions The results presented herein inform that Porphyromonas spp. isolated from dogs with and without periodontitis harbored the prtC and fimA genes and it could be a role in the establishment of the infectious process. |
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Pathogenicity and genetic profile of oral Porphyromonas species from canine periodontitisfimA geneGenetic diversityPeriodontal diseasePorphyromonas spp.prtC geneObjective In this study, the presence of the prtC and fimA genes involved in the pathogenicity of oral Porphyromonas spp. isolated from dogs with periodontitis and healthy, as well as their genetic diversity was investigated. Design Thirty-two Beagle dogs, 24 with periodontitis and 8 healthy were evaluated. Subgingival samples from only one gingival site of both groups were collected. Bacteria grown in anaerobiosis were identified by RAPID ID 32A kits. From each strain the respective DNA was obtained and used to genotyping by conventional PCR and AP-PCR. Results Dogs with periodontitis harbored 28 P. gulae, 2 P. creviocaricanis, 1 P. cangingivalis and 7 P. macacae; and from healthy dogs, 11 P. gulae and 5 P. circumdentaria. In P. gulae isolated from periodontal dogs the gene prtC was observed in 19 (67.85%) and in 7 (63.63%) from healthy dogs. P. gulae strains from periodontal dogs harbored either the gene fimA I or fimA II; while strains from healthy dogs harbored the gene fimA I, fimA II, fimA III or fimA IV, as well as 1 P. circumdentaria the gene fimA II. By AP-PCR strains were grouped in different clusters suggesting heterogeneity of these microorganisms. Conclusions The results presented herein inform that Porphyromonas spp. isolated from dogs with and without periodontitis harbored the prtC and fimA genes and it could be a role in the establishment of the infectious process.Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)Anaerobe Laboratory Department of Microbiology Institute of Biomedical Sciences University of Sao Paulo – USPDepartment of Dental Materials and Prosthodontics School of Dentistry of Araraquara Univ Estadual Paulista – UNESPDepartment of Dental Materials and Prosthodontics School of Dentistry of Araraquara Univ Estadual Paulista – UNESPFAPESP: 2013/13652-6Universidade de São Paulo (USP)Universidade Estadual Paulista (Unesp)do Nascimento Silva, Amandade Avila, Erica Dorigatti [UNESP]Nakano, VivianeAvila-Campos, Mario J.2018-12-11T17:13:10Z2018-12-11T17:13:10Z2017-11-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article20-24application/pdfhttp://dx.doi.org/10.1016/j.archoralbio.2017.07.001Archives of Oral Biology, v. 83, p. 20-24.1879-15060003-9969http://hdl.handle.net/11449/17485810.1016/j.archoralbio.2017.07.0012-s2.0-850219730522-s2.0-85021973052.pdfScopusreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengArchives of Oral Biology0,752info:eu-repo/semantics/openAccess2024-01-18T06:32:52Zoai:repositorio.unesp.br:11449/174858Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462024-01-18T06:32:52Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false |
dc.title.none.fl_str_mv |
Pathogenicity and genetic profile of oral Porphyromonas species from canine periodontitis |
title |
Pathogenicity and genetic profile of oral Porphyromonas species from canine periodontitis |
spellingShingle |
Pathogenicity and genetic profile of oral Porphyromonas species from canine periodontitis do Nascimento Silva, Amanda fimA gene Genetic diversity Periodontal disease Porphyromonas spp. prtC gene |
title_short |
Pathogenicity and genetic profile of oral Porphyromonas species from canine periodontitis |
title_full |
Pathogenicity and genetic profile of oral Porphyromonas species from canine periodontitis |
title_fullStr |
Pathogenicity and genetic profile of oral Porphyromonas species from canine periodontitis |
title_full_unstemmed |
Pathogenicity and genetic profile of oral Porphyromonas species from canine periodontitis |
title_sort |
Pathogenicity and genetic profile of oral Porphyromonas species from canine periodontitis |
author |
do Nascimento Silva, Amanda |
author_facet |
do Nascimento Silva, Amanda de Avila, Erica Dorigatti [UNESP] Nakano, Viviane Avila-Campos, Mario J. |
author_role |
author |
author2 |
de Avila, Erica Dorigatti [UNESP] Nakano, Viviane Avila-Campos, Mario J. |
author2_role |
author author author |
dc.contributor.none.fl_str_mv |
Universidade de São Paulo (USP) Universidade Estadual Paulista (Unesp) |
dc.contributor.author.fl_str_mv |
do Nascimento Silva, Amanda de Avila, Erica Dorigatti [UNESP] Nakano, Viviane Avila-Campos, Mario J. |
dc.subject.por.fl_str_mv |
fimA gene Genetic diversity Periodontal disease Porphyromonas spp. prtC gene |
topic |
fimA gene Genetic diversity Periodontal disease Porphyromonas spp. prtC gene |
description |
Objective In this study, the presence of the prtC and fimA genes involved in the pathogenicity of oral Porphyromonas spp. isolated from dogs with periodontitis and healthy, as well as their genetic diversity was investigated. Design Thirty-two Beagle dogs, 24 with periodontitis and 8 healthy were evaluated. Subgingival samples from only one gingival site of both groups were collected. Bacteria grown in anaerobiosis were identified by RAPID ID 32A kits. From each strain the respective DNA was obtained and used to genotyping by conventional PCR and AP-PCR. Results Dogs with periodontitis harbored 28 P. gulae, 2 P. creviocaricanis, 1 P. cangingivalis and 7 P. macacae; and from healthy dogs, 11 P. gulae and 5 P. circumdentaria. In P. gulae isolated from periodontal dogs the gene prtC was observed in 19 (67.85%) and in 7 (63.63%) from healthy dogs. P. gulae strains from periodontal dogs harbored either the gene fimA I or fimA II; while strains from healthy dogs harbored the gene fimA I, fimA II, fimA III or fimA IV, as well as 1 P. circumdentaria the gene fimA II. By AP-PCR strains were grouped in different clusters suggesting heterogeneity of these microorganisms. Conclusions The results presented herein inform that Porphyromonas spp. isolated from dogs with and without periodontitis harbored the prtC and fimA genes and it could be a role in the establishment of the infectious process. |
publishDate |
2017 |
dc.date.none.fl_str_mv |
2017-11-01 2018-12-11T17:13:10Z 2018-12-11T17:13:10Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://dx.doi.org/10.1016/j.archoralbio.2017.07.001 Archives of Oral Biology, v. 83, p. 20-24. 1879-1506 0003-9969 http://hdl.handle.net/11449/174858 10.1016/j.archoralbio.2017.07.001 2-s2.0-85021973052 2-s2.0-85021973052.pdf |
url |
http://dx.doi.org/10.1016/j.archoralbio.2017.07.001 http://hdl.handle.net/11449/174858 |
identifier_str_mv |
Archives of Oral Biology, v. 83, p. 20-24. 1879-1506 0003-9969 10.1016/j.archoralbio.2017.07.001 2-s2.0-85021973052 2-s2.0-85021973052.pdf |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Archives of Oral Biology 0,752 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
20-24 application/pdf |
dc.source.none.fl_str_mv |
Scopus reponame:Repositório Institucional da UNESP instname:Universidade Estadual Paulista (UNESP) instacron:UNESP |
instname_str |
Universidade Estadual Paulista (UNESP) |
instacron_str |
UNESP |
institution |
UNESP |
reponame_str |
Repositório Institucional da UNESP |
collection |
Repositório Institucional da UNESP |
repository.name.fl_str_mv |
Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP) |
repository.mail.fl_str_mv |
|
_version_ |
1799965660360474624 |