Pathogenicity and genetic profile of oral Porphyromonas species from canine periodontitis

Detalhes bibliográficos
Autor(a) principal: do Nascimento Silva, Amanda
Data de Publicação: 2017
Outros Autores: de Avila, Erica Dorigatti [UNESP], Nakano, Viviane, Avila-Campos, Mario J.
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da UNESP
Texto Completo: http://dx.doi.org/10.1016/j.archoralbio.2017.07.001
http://hdl.handle.net/11449/174858
Resumo: Objective In this study, the presence of the prtC and fimA genes involved in the pathogenicity of oral Porphyromonas spp. isolated from dogs with periodontitis and healthy, as well as their genetic diversity was investigated. Design Thirty-two Beagle dogs, 24 with periodontitis and 8 healthy were evaluated. Subgingival samples from only one gingival site of both groups were collected. Bacteria grown in anaerobiosis were identified by RAPID ID 32A kits. From each strain the respective DNA was obtained and used to genotyping by conventional PCR and AP-PCR. Results Dogs with periodontitis harbored 28 P. gulae, 2 P. creviocaricanis, 1 P. cangingivalis and 7 P. macacae; and from healthy dogs, 11 P. gulae and 5 P. circumdentaria. In P. gulae isolated from periodontal dogs the gene prtC was observed in 19 (67.85%) and in 7 (63.63%) from healthy dogs. P. gulae strains from periodontal dogs harbored either the gene fimA I or fimA II; while strains from healthy dogs harbored the gene fimA I, fimA II, fimA III or fimA IV, as well as 1 P. circumdentaria the gene fimA II. By AP-PCR strains were grouped in different clusters suggesting heterogeneity of these microorganisms. Conclusions The results presented herein inform that Porphyromonas spp. isolated from dogs with and without periodontitis harbored the prtC and fimA genes and it could be a role in the establishment of the infectious process.
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spelling Pathogenicity and genetic profile of oral Porphyromonas species from canine periodontitisfimA geneGenetic diversityPeriodontal diseasePorphyromonas spp.prtC geneObjective In this study, the presence of the prtC and fimA genes involved in the pathogenicity of oral Porphyromonas spp. isolated from dogs with periodontitis and healthy, as well as their genetic diversity was investigated. Design Thirty-two Beagle dogs, 24 with periodontitis and 8 healthy were evaluated. Subgingival samples from only one gingival site of both groups were collected. Bacteria grown in anaerobiosis were identified by RAPID ID 32A kits. From each strain the respective DNA was obtained and used to genotyping by conventional PCR and AP-PCR. Results Dogs with periodontitis harbored 28 P. gulae, 2 P. creviocaricanis, 1 P. cangingivalis and 7 P. macacae; and from healthy dogs, 11 P. gulae and 5 P. circumdentaria. In P. gulae isolated from periodontal dogs the gene prtC was observed in 19 (67.85%) and in 7 (63.63%) from healthy dogs. P. gulae strains from periodontal dogs harbored either the gene fimA I or fimA II; while strains from healthy dogs harbored the gene fimA I, fimA II, fimA III or fimA IV, as well as 1 P. circumdentaria the gene fimA II. By AP-PCR strains were grouped in different clusters suggesting heterogeneity of these microorganisms. Conclusions The results presented herein inform that Porphyromonas spp. isolated from dogs with and without periodontitis harbored the prtC and fimA genes and it could be a role in the establishment of the infectious process.Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)Anaerobe Laboratory Department of Microbiology Institute of Biomedical Sciences University of Sao Paulo – USPDepartment of Dental Materials and Prosthodontics School of Dentistry of Araraquara Univ Estadual Paulista – UNESPDepartment of Dental Materials and Prosthodontics School of Dentistry of Araraquara Univ Estadual Paulista – UNESPFAPESP: 2013/13652-6Universidade de São Paulo (USP)Universidade Estadual Paulista (Unesp)do Nascimento Silva, Amandade Avila, Erica Dorigatti [UNESP]Nakano, VivianeAvila-Campos, Mario J.2018-12-11T17:13:10Z2018-12-11T17:13:10Z2017-11-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article20-24application/pdfhttp://dx.doi.org/10.1016/j.archoralbio.2017.07.001Archives of Oral Biology, v. 83, p. 20-24.1879-15060003-9969http://hdl.handle.net/11449/17485810.1016/j.archoralbio.2017.07.0012-s2.0-850219730522-s2.0-85021973052.pdfScopusreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengArchives of Oral Biology0,752info:eu-repo/semantics/openAccess2024-01-18T06:32:52Zoai:repositorio.unesp.br:11449/174858Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462024-01-18T06:32:52Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false
dc.title.none.fl_str_mv Pathogenicity and genetic profile of oral Porphyromonas species from canine periodontitis
title Pathogenicity and genetic profile of oral Porphyromonas species from canine periodontitis
spellingShingle Pathogenicity and genetic profile of oral Porphyromonas species from canine periodontitis
do Nascimento Silva, Amanda
fimA gene
Genetic diversity
Periodontal disease
Porphyromonas spp.
prtC gene
title_short Pathogenicity and genetic profile of oral Porphyromonas species from canine periodontitis
title_full Pathogenicity and genetic profile of oral Porphyromonas species from canine periodontitis
title_fullStr Pathogenicity and genetic profile of oral Porphyromonas species from canine periodontitis
title_full_unstemmed Pathogenicity and genetic profile of oral Porphyromonas species from canine periodontitis
title_sort Pathogenicity and genetic profile of oral Porphyromonas species from canine periodontitis
author do Nascimento Silva, Amanda
author_facet do Nascimento Silva, Amanda
de Avila, Erica Dorigatti [UNESP]
Nakano, Viviane
Avila-Campos, Mario J.
author_role author
author2 de Avila, Erica Dorigatti [UNESP]
Nakano, Viviane
Avila-Campos, Mario J.
author2_role author
author
author
dc.contributor.none.fl_str_mv Universidade de São Paulo (USP)
Universidade Estadual Paulista (Unesp)
dc.contributor.author.fl_str_mv do Nascimento Silva, Amanda
de Avila, Erica Dorigatti [UNESP]
Nakano, Viviane
Avila-Campos, Mario J.
dc.subject.por.fl_str_mv fimA gene
Genetic diversity
Periodontal disease
Porphyromonas spp.
prtC gene
topic fimA gene
Genetic diversity
Periodontal disease
Porphyromonas spp.
prtC gene
description Objective In this study, the presence of the prtC and fimA genes involved in the pathogenicity of oral Porphyromonas spp. isolated from dogs with periodontitis and healthy, as well as their genetic diversity was investigated. Design Thirty-two Beagle dogs, 24 with periodontitis and 8 healthy were evaluated. Subgingival samples from only one gingival site of both groups were collected. Bacteria grown in anaerobiosis were identified by RAPID ID 32A kits. From each strain the respective DNA was obtained and used to genotyping by conventional PCR and AP-PCR. Results Dogs with periodontitis harbored 28 P. gulae, 2 P. creviocaricanis, 1 P. cangingivalis and 7 P. macacae; and from healthy dogs, 11 P. gulae and 5 P. circumdentaria. In P. gulae isolated from periodontal dogs the gene prtC was observed in 19 (67.85%) and in 7 (63.63%) from healthy dogs. P. gulae strains from periodontal dogs harbored either the gene fimA I or fimA II; while strains from healthy dogs harbored the gene fimA I, fimA II, fimA III or fimA IV, as well as 1 P. circumdentaria the gene fimA II. By AP-PCR strains were grouped in different clusters suggesting heterogeneity of these microorganisms. Conclusions The results presented herein inform that Porphyromonas spp. isolated from dogs with and without periodontitis harbored the prtC and fimA genes and it could be a role in the establishment of the infectious process.
publishDate 2017
dc.date.none.fl_str_mv 2017-11-01
2018-12-11T17:13:10Z
2018-12-11T17:13:10Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://dx.doi.org/10.1016/j.archoralbio.2017.07.001
Archives of Oral Biology, v. 83, p. 20-24.
1879-1506
0003-9969
http://hdl.handle.net/11449/174858
10.1016/j.archoralbio.2017.07.001
2-s2.0-85021973052
2-s2.0-85021973052.pdf
url http://dx.doi.org/10.1016/j.archoralbio.2017.07.001
http://hdl.handle.net/11449/174858
identifier_str_mv Archives of Oral Biology, v. 83, p. 20-24.
1879-1506
0003-9969
10.1016/j.archoralbio.2017.07.001
2-s2.0-85021973052
2-s2.0-85021973052.pdf
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv Archives of Oral Biology
0,752
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv 20-24
application/pdf
dc.source.none.fl_str_mv Scopus
reponame:Repositório Institucional da UNESP
instname:Universidade Estadual Paulista (UNESP)
instacron:UNESP
instname_str Universidade Estadual Paulista (UNESP)
instacron_str UNESP
institution UNESP
reponame_str Repositório Institucional da UNESP
collection Repositório Institucional da UNESP
repository.name.fl_str_mv Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)
repository.mail.fl_str_mv
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