Polymerase chain reaction evaluation for canine visceral leishmaniasis diagnosis in dog blood samples
Autor(a) principal: | |
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Data de Publicação: | 2007 |
Outros Autores: | , , , , , |
Tipo de documento: | Artigo |
Idioma: | por |
Título da fonte: | Repositório Institucional da UNESP |
Texto Completo: | http://hdl.handle.net/11449/224978 |
Resumo: | Canine and human visceral leishmaniasis is endemic in several States of Brazil, and it is associated with infected dogs and the presence of the vector. Aiming at using polymerase chain reaction as a diagnostic tool in dogs, we amplified a 120bp fragment from kDNA of Leishmania spp. by PCR in blood samples. The lower detection limit observed was 0.1 parasites per 500microL of blood, which is a highly satisfactory result. On the other hand, PCR evaluation in 166 blood samples of dogs from Poxoréo, MS, Brazil, resulted in 55% sensitivity and 66.3% specificity, considering indirect imunnofluorescent test as gold standard. |
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Repositório Institucional da UNESP |
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spelling |
Polymerase chain reaction evaluation for canine visceral leishmaniasis diagnosis in dog blood samplesAvaliação da reação em cadeia pela polimerase para diagnóstico da leishmaniose visceral em sangue de cães.Canine and human visceral leishmaniasis is endemic in several States of Brazil, and it is associated with infected dogs and the presence of the vector. Aiming at using polymerase chain reaction as a diagnostic tool in dogs, we amplified a 120bp fragment from kDNA of Leishmania spp. by PCR in blood samples. The lower detection limit observed was 0.1 parasites per 500microL of blood, which is a highly satisfactory result. On the other hand, PCR evaluation in 166 blood samples of dogs from Poxoréo, MS, Brazil, resulted in 55% sensitivity and 66.3% specificity, considering indirect imunnofluorescent test as gold standard.Rua Clóvis Pestana 93 Curso de Medicina Veterinária Departamento de Apoio Produção e Saúde Animal Universidade Estadual Paulista Júlio de Mesquita Filho (UNESP) Campus de Araçatuba AraçatubaRua Clóvis Pestana 93 Curso de Medicina Veterinária Departamento de Apoio Produção e Saúde Animal Universidade Estadual Paulista Júlio de Mesquita Filho (UNESP) Campus de Araçatuba AraçatubaUniversidade Estadual Paulista (UNESP)Nunes, Cáris M [UNESP]Dias, Ana Karina K [UNESP]Gottardi, Fernanda Patricia [UNESP]De Paula, Henrique B [UNESP]De Azevedo, Márcia A A [UNESP]De Lima, Valéria M F [UNESP]Garcia, José Fernando [UNESP]2022-04-28T20:22:08Z2022-04-28T20:22:08Z2007-01-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article5-9Revista brasileira de parasitologia veterinária = Brazilian journal of veterinary parasitology : Órgão Oficial do Colégio Brasileiro de Parasitologia Veterinária, v. 16, n. 1, p. 5-9, 2007.0103-846Xhttp://hdl.handle.net/11449/2249782-s2.0-35548944536Scopusreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPporRevista brasileira de parasitologia veterinária = Brazilian journal of veterinary parasitology : Órgão Oficial do Colégio Brasileiro de Parasitologia Veterináriainfo:eu-repo/semantics/openAccess2024-09-04T19:15:25Zoai:repositorio.unesp.br:11449/224978Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestrepositoriounesp@unesp.bropendoar:29462024-09-04T19:15:25Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false |
dc.title.none.fl_str_mv |
Polymerase chain reaction evaluation for canine visceral leishmaniasis diagnosis in dog blood samples Avaliação da reação em cadeia pela polimerase para diagnóstico da leishmaniose visceral em sangue de cães. |
title |
Polymerase chain reaction evaluation for canine visceral leishmaniasis diagnosis in dog blood samples |
spellingShingle |
Polymerase chain reaction evaluation for canine visceral leishmaniasis diagnosis in dog blood samples Nunes, Cáris M [UNESP] |
title_short |
Polymerase chain reaction evaluation for canine visceral leishmaniasis diagnosis in dog blood samples |
title_full |
Polymerase chain reaction evaluation for canine visceral leishmaniasis diagnosis in dog blood samples |
title_fullStr |
Polymerase chain reaction evaluation for canine visceral leishmaniasis diagnosis in dog blood samples |
title_full_unstemmed |
Polymerase chain reaction evaluation for canine visceral leishmaniasis diagnosis in dog blood samples |
title_sort |
Polymerase chain reaction evaluation for canine visceral leishmaniasis diagnosis in dog blood samples |
author |
Nunes, Cáris M [UNESP] |
author_facet |
Nunes, Cáris M [UNESP] Dias, Ana Karina K [UNESP] Gottardi, Fernanda Patricia [UNESP] De Paula, Henrique B [UNESP] De Azevedo, Márcia A A [UNESP] De Lima, Valéria M F [UNESP] Garcia, José Fernando [UNESP] |
author_role |
author |
author2 |
Dias, Ana Karina K [UNESP] Gottardi, Fernanda Patricia [UNESP] De Paula, Henrique B [UNESP] De Azevedo, Márcia A A [UNESP] De Lima, Valéria M F [UNESP] Garcia, José Fernando [UNESP] |
author2_role |
author author author author author author |
dc.contributor.none.fl_str_mv |
Universidade Estadual Paulista (UNESP) |
dc.contributor.author.fl_str_mv |
Nunes, Cáris M [UNESP] Dias, Ana Karina K [UNESP] Gottardi, Fernanda Patricia [UNESP] De Paula, Henrique B [UNESP] De Azevedo, Márcia A A [UNESP] De Lima, Valéria M F [UNESP] Garcia, José Fernando [UNESP] |
description |
Canine and human visceral leishmaniasis is endemic in several States of Brazil, and it is associated with infected dogs and the presence of the vector. Aiming at using polymerase chain reaction as a diagnostic tool in dogs, we amplified a 120bp fragment from kDNA of Leishmania spp. by PCR in blood samples. The lower detection limit observed was 0.1 parasites per 500microL of blood, which is a highly satisfactory result. On the other hand, PCR evaluation in 166 blood samples of dogs from Poxoréo, MS, Brazil, resulted in 55% sensitivity and 66.3% specificity, considering indirect imunnofluorescent test as gold standard. |
publishDate |
2007 |
dc.date.none.fl_str_mv |
2007-01-01 2022-04-28T20:22:08Z 2022-04-28T20:22:08Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
Revista brasileira de parasitologia veterinária = Brazilian journal of veterinary parasitology : Órgão Oficial do Colégio Brasileiro de Parasitologia Veterinária, v. 16, n. 1, p. 5-9, 2007. 0103-846X http://hdl.handle.net/11449/224978 2-s2.0-35548944536 |
identifier_str_mv |
Revista brasileira de parasitologia veterinária = Brazilian journal of veterinary parasitology : Órgão Oficial do Colégio Brasileiro de Parasitologia Veterinária, v. 16, n. 1, p. 5-9, 2007. 0103-846X 2-s2.0-35548944536 |
url |
http://hdl.handle.net/11449/224978 |
dc.language.iso.fl_str_mv |
por |
language |
por |
dc.relation.none.fl_str_mv |
Revista brasileira de parasitologia veterinária = Brazilian journal of veterinary parasitology : Órgão Oficial do Colégio Brasileiro de Parasitologia Veterinária |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
5-9 |
dc.source.none.fl_str_mv |
Scopus reponame:Repositório Institucional da UNESP instname:Universidade Estadual Paulista (UNESP) instacron:UNESP |
instname_str |
Universidade Estadual Paulista (UNESP) |
instacron_str |
UNESP |
institution |
UNESP |
reponame_str |
Repositório Institucional da UNESP |
collection |
Repositório Institucional da UNESP |
repository.name.fl_str_mv |
Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP) |
repository.mail.fl_str_mv |
repositoriounesp@unesp.br |
_version_ |
1810021381571608576 |