Evaluation of ERIC-PCR as Genotyping Method for Corynebacterium pseudotuberculosis Isolates

• Autor(a) principal: Dorneles, Elaine M. S.
• Data de Publicação: 2014
• Outros Autores: Santana, Jordana A., Ribeiro, Dayana, Dorella, Fernanda Alves, Guimaraes, Alessandro S., Moawad, Mohamed S., Selim, Salah A., Garaldi, Ana Luiza M., Miyoshi, Anderson, Ribeiro, Márcio Garcia [UNESP], Gouveia, Aurora M. G., Azevedo, Vasco, Heinemann, Marcos B., Lage, Andrey P.
• Tipo de documento: Artigo
• Idioma: eng
• Título da fonte: Repositório Institucional da UNESP
• Texto Completo: http://dx.doi.org/10.1371/journal.pone.0098758; http://hdl.handle.net/11449/112349

The aim of this study was to evaluate the Enterobacterial Repetitive Intergenic Consensus (ERIC-PCR) as a tool for molecular typing of C. pseudotuberculosis isolates from eight different hosts in twelve countries. Ninety-nine C. pseudotuberculosis field strains, one type strain (ATCC 19410(T)) and one vaccine strain (1002) were fingerprinted using the ERIC-1R and ERIC-2 primers, and the ERIC-1R+ERIC-2 primer pair. Twenty-nine different genotypes were generated by ERIC 1-PCR, 28 by ERIC 2-PCR and 35 by ERIC 1+2-PCR. The discriminatory index calculated for ERIC 1, ERIC 2, and ERIC 1+2-PCR was 0.89, 0.86, and 0.92, respectively. Epidemiological concordance was established for all ERIC-PCR assays. ERIC 1+2-PCR was defined as the best method based on suitability of the amplification patterns and discriminatory index. Minimal spanning tree for ERIC 1+2-PCR revealed three major clonal complexes and clustering around nitrate-positive (biovar Equi) and nitrate-negative (biovar Ovis) strains. Therefore, ERIC 1+2-PCR proved to be the best technique evaluated in this study for genotyping C. pseudotuberculosis strains, due to its usefulness for molecular epidemiology investigations.