Evaluation of ERIC-PCR as Genotyping Method for Corynebacterium pseudotuberculosis Isolates

Detalhes bibliográficos
Autor(a) principal: Dorneles, Elaine M. S.
Data de Publicação: 2014
Outros Autores: Santana, Jordana A., Ribeiro, Dayana, Dorella, Fernanda Alves, Guimaraes, Alessandro S., Moawad, Mohamed S., Selim, Salah A., Garaldi, Ana Luiza M., Miyoshi, Anderson, Ribeiro, Márcio Garcia [UNESP], Gouveia, Aurora M. G., Azevedo, Vasco, Heinemann, Marcos B., Lage, Andrey P.
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da UNESP
Texto Completo: http://dx.doi.org/10.1371/journal.pone.0098758
http://hdl.handle.net/11449/112349
Resumo: The aim of this study was to evaluate the Enterobacterial Repetitive Intergenic Consensus (ERIC-PCR) as a tool for molecular typing of C. pseudotuberculosis isolates from eight different hosts in twelve countries. Ninety-nine C. pseudotuberculosis field strains, one type strain (ATCC 19410(T)) and one vaccine strain (1002) were fingerprinted using the ERIC-1R and ERIC-2 primers, and the ERIC-1R+ERIC-2 primer pair. Twenty-nine different genotypes were generated by ERIC 1-PCR, 28 by ERIC 2-PCR and 35 by ERIC 1+2-PCR. The discriminatory index calculated for ERIC 1, ERIC 2, and ERIC 1+2-PCR was 0.89, 0.86, and 0.92, respectively. Epidemiological concordance was established for all ERIC-PCR assays. ERIC 1+2-PCR was defined as the best method based on suitability of the amplification patterns and discriminatory index. Minimal spanning tree for ERIC 1+2-PCR revealed three major clonal complexes and clustering around nitrate-positive (biovar Equi) and nitrate-negative (biovar Ovis) strains. Therefore, ERIC 1+2-PCR proved to be the best technique evaluated in this study for genotyping C. pseudotuberculosis strains, due to its usefulness for molecular epidemiology investigations.
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spelling Evaluation of ERIC-PCR as Genotyping Method for Corynebacterium pseudotuberculosis IsolatesThe aim of this study was to evaluate the Enterobacterial Repetitive Intergenic Consensus (ERIC-PCR) as a tool for molecular typing of C. pseudotuberculosis isolates from eight different hosts in twelve countries. Ninety-nine C. pseudotuberculosis field strains, one type strain (ATCC 19410(T)) and one vaccine strain (1002) were fingerprinted using the ERIC-1R and ERIC-2 primers, and the ERIC-1R+ERIC-2 primer pair. Twenty-nine different genotypes were generated by ERIC 1-PCR, 28 by ERIC 2-PCR and 35 by ERIC 1+2-PCR. The discriminatory index calculated for ERIC 1, ERIC 2, and ERIC 1+2-PCR was 0.89, 0.86, and 0.92, respectively. Epidemiological concordance was established for all ERIC-PCR assays. ERIC 1+2-PCR was defined as the best method based on suitability of the amplification patterns and discriminatory index. Minimal spanning tree for ERIC 1+2-PCR revealed three major clonal complexes and clustering around nitrate-positive (biovar Equi) and nitrate-negative (biovar Ovis) strains. Therefore, ERIC 1+2-PCR proved to be the best technique evaluated in this study for genotyping C. pseudotuberculosis strains, due to its usefulness for molecular epidemiology investigations.Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)Fundação de Amparo à Pesquisa do Estado de Minas Gerais (FAPEMIG)Fundacao de Estudo e Pesquisa em Medicina Veterinaria e Zootecnia - FEP-MVZUniv Fed Minas Gerais, Dept Med Vet Prevent, Escola Vet, Belo Horizonte, MG, BrazilUniv Fed Minas Gerais, Inst Ciencias Biol, Dept Biol Geral, Belo Horizonte, MG, BrazilEmpresa Brasileira Pesquisa Agr, Embrapa Gado Leite, Juiz De Fora, BrazilUniv Fed Lavras, Dept Vet Med, Lavras, MG, BrazilCairo Univ, Dept Toxicol & Forens Med, Cairo, EgyptUniv Estado Rio de Janeiro, Ctr Biomed, Fac Ciencias Med, BR-20550011 Rio De Janeiro, BrazilUniv Estadual Paulista, Dept Higiene Vet & Saude Publ, Fac Med Vet & Zootecnia, Botucatu, SP, BrazilUniv Estadual Paulista, Dept Higiene Vet & Saude Publ, Fac Med Vet & Zootecnia, Botucatu, SP, BrazilPublic Library ScienceUniversidade Federal de Minas Gerais (UFMG)Empresa Brasileira de Pesquisa Agropecuária (EMBRAPA)Universidade Federal de Lavras (UFLA)Cairo UnivUniversidade do Estado do Rio de Janeiro (UERJ)Universidade Estadual Paulista (Unesp)Dorneles, Elaine M. S.Santana, Jordana A.Ribeiro, DayanaDorella, Fernanda AlvesGuimaraes, Alessandro S.Moawad, Mohamed S.Selim, Salah A.Garaldi, Ana Luiza M.Miyoshi, AndersonRibeiro, Márcio Garcia [UNESP]Gouveia, Aurora M. G.Azevedo, VascoHeinemann, Marcos B.Lage, Andrey P.2014-12-03T13:10:38Z2014-12-03T13:10:38Z2014-06-05info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article10application/pdfhttp://dx.doi.org/10.1371/journal.pone.0098758Plos One. San Francisco: Public Library Science, v. 9, n. 6, 10 p., 2014.1932-6203http://hdl.handle.net/11449/11234910.1371/journal.pone.0098758WOS:000336841400049WOS000336841400049.pdf2209124317273797Web of Sciencereponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengPLOS ONE2.7661,164info:eu-repo/semantics/openAccess2023-12-07T06:21:50Zoai:repositorio.unesp.br:11449/112349Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462024-08-05T19:44:36.503492Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false
dc.title.none.fl_str_mv Evaluation of ERIC-PCR as Genotyping Method for Corynebacterium pseudotuberculosis Isolates
title Evaluation of ERIC-PCR as Genotyping Method for Corynebacterium pseudotuberculosis Isolates
spellingShingle Evaluation of ERIC-PCR as Genotyping Method for Corynebacterium pseudotuberculosis Isolates
Dorneles, Elaine M. S.
title_short Evaluation of ERIC-PCR as Genotyping Method for Corynebacterium pseudotuberculosis Isolates
title_full Evaluation of ERIC-PCR as Genotyping Method for Corynebacterium pseudotuberculosis Isolates
title_fullStr Evaluation of ERIC-PCR as Genotyping Method for Corynebacterium pseudotuberculosis Isolates
title_full_unstemmed Evaluation of ERIC-PCR as Genotyping Method for Corynebacterium pseudotuberculosis Isolates
title_sort Evaluation of ERIC-PCR as Genotyping Method for Corynebacterium pseudotuberculosis Isolates
author Dorneles, Elaine M. S.
author_facet Dorneles, Elaine M. S.
Santana, Jordana A.
Ribeiro, Dayana
Dorella, Fernanda Alves
Guimaraes, Alessandro S.
Moawad, Mohamed S.
Selim, Salah A.
Garaldi, Ana Luiza M.
Miyoshi, Anderson
Ribeiro, Márcio Garcia [UNESP]
Gouveia, Aurora M. G.
Azevedo, Vasco
Heinemann, Marcos B.
Lage, Andrey P.
author_role author
author2 Santana, Jordana A.
Ribeiro, Dayana
Dorella, Fernanda Alves
Guimaraes, Alessandro S.
Moawad, Mohamed S.
Selim, Salah A.
Garaldi, Ana Luiza M.
Miyoshi, Anderson
Ribeiro, Márcio Garcia [UNESP]
Gouveia, Aurora M. G.
Azevedo, Vasco
Heinemann, Marcos B.
Lage, Andrey P.
author2_role author
author
author
author
author
author
author
author
author
author
author
author
author
dc.contributor.none.fl_str_mv Universidade Federal de Minas Gerais (UFMG)
Empresa Brasileira de Pesquisa Agropecuária (EMBRAPA)
Universidade Federal de Lavras (UFLA)
Cairo Univ
Universidade do Estado do Rio de Janeiro (UERJ)
Universidade Estadual Paulista (Unesp)
dc.contributor.author.fl_str_mv Dorneles, Elaine M. S.
Santana, Jordana A.
Ribeiro, Dayana
Dorella, Fernanda Alves
Guimaraes, Alessandro S.
Moawad, Mohamed S.
Selim, Salah A.
Garaldi, Ana Luiza M.
Miyoshi, Anderson
Ribeiro, Márcio Garcia [UNESP]
Gouveia, Aurora M. G.
Azevedo, Vasco
Heinemann, Marcos B.
Lage, Andrey P.
description The aim of this study was to evaluate the Enterobacterial Repetitive Intergenic Consensus (ERIC-PCR) as a tool for molecular typing of C. pseudotuberculosis isolates from eight different hosts in twelve countries. Ninety-nine C. pseudotuberculosis field strains, one type strain (ATCC 19410(T)) and one vaccine strain (1002) were fingerprinted using the ERIC-1R and ERIC-2 primers, and the ERIC-1R+ERIC-2 primer pair. Twenty-nine different genotypes were generated by ERIC 1-PCR, 28 by ERIC 2-PCR and 35 by ERIC 1+2-PCR. The discriminatory index calculated for ERIC 1, ERIC 2, and ERIC 1+2-PCR was 0.89, 0.86, and 0.92, respectively. Epidemiological concordance was established for all ERIC-PCR assays. ERIC 1+2-PCR was defined as the best method based on suitability of the amplification patterns and discriminatory index. Minimal spanning tree for ERIC 1+2-PCR revealed three major clonal complexes and clustering around nitrate-positive (biovar Equi) and nitrate-negative (biovar Ovis) strains. Therefore, ERIC 1+2-PCR proved to be the best technique evaluated in this study for genotyping C. pseudotuberculosis strains, due to its usefulness for molecular epidemiology investigations.
publishDate 2014
dc.date.none.fl_str_mv 2014-12-03T13:10:38Z
2014-12-03T13:10:38Z
2014-06-05
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://dx.doi.org/10.1371/journal.pone.0098758
Plos One. San Francisco: Public Library Science, v. 9, n. 6, 10 p., 2014.
1932-6203
http://hdl.handle.net/11449/112349
10.1371/journal.pone.0098758
WOS:000336841400049
WOS000336841400049.pdf
2209124317273797
url http://dx.doi.org/10.1371/journal.pone.0098758
http://hdl.handle.net/11449/112349
identifier_str_mv Plos One. San Francisco: Public Library Science, v. 9, n. 6, 10 p., 2014.
1932-6203
10.1371/journal.pone.0098758
WOS:000336841400049
WOS000336841400049.pdf
2209124317273797
dc.language.iso.fl_str_mv eng
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publisher.none.fl_str_mv Public Library Science
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