Biocompatibility of a chlorhexidine local delivery system in a subcutaneous mouse model

Detalhes bibliográficos
Autor(a) principal: Monteiro, Adriana-Socorro-Ferreira [UNESP]
Data de Publicação: 2011
Outros Autores: Macedo, Luis-Guilherme-Scavone [UNESP], Macedo, Nelson-Luiz [UNESP], Feitosa, Fernanda-Alves, Toyoshima, Thiago [UNESP]
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da UNESP
Texto Completo: http://dx.doi.org/10.4317/medoral.16.e278
http://hdl.handle.net/11449/22734
Resumo: Objective: This study aimed evaluating histologically and histomorphometrically the response of the conjunctive tissue face to the implant of chlorhexidine chips in the subcutaneous tissues of rats. Study Design: In this research 35 male rats Wistar were used to analyze the biocompatibility and the degradation process of chlorhexidine chip. In each animal, it was made 2 incisions for subcutaneous implantation of chlorhexidine chip (test group) and a polytetrafluorethylene membrane (control group). The morphological changes in subcutaneous implantations were assessed after 1, 3, 5, 7, 10, 14, 21 days. The data were submitted to Friedman nonparametric test to analyze the comparisons among observation periods and to allow the comparison among groups. Results: Differences were found in the analysis of the inflammatory response when comparing the tested materials (p values <= 0.05). In test group was observed hemorrhage, edema and intense inflammatory infiltrate predominantly neutrophilic around material. From 3-day and subsequent periods was verified granulation tissue externally at this infiltrate. From 10-day on was observed crescent area of degradation of chlorhexidine chip, associated with neutrophilic and macrophagic infiltrate, that maintained until 21-day. In the control group, moderate inflammatory infiltrate was observed initially, predominantly polymorphonuclear, edema and granulation tissue 3-day period. The inflammatory infiltrate was gradually replaced for granulation tissue, culminating in a fibrous capsule. Giant multinucleate cells situated at contact interface with the coating was examined since 3-day and persisted until 21-day. Conclusion: The chlorhexidine chip induces an intense acute inflammatory response at subcutaneous tissue of rats. Therefore, at conditions of this study was not biocompatible.
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spelling Biocompatibility of a chlorhexidine local delivery system in a subcutaneous mouse modelBiocompatibilitychlorhexidine toxicitydrug delivery systemperiodontitisObjective: This study aimed evaluating histologically and histomorphometrically the response of the conjunctive tissue face to the implant of chlorhexidine chips in the subcutaneous tissues of rats. Study Design: In this research 35 male rats Wistar were used to analyze the biocompatibility and the degradation process of chlorhexidine chip. In each animal, it was made 2 incisions for subcutaneous implantation of chlorhexidine chip (test group) and a polytetrafluorethylene membrane (control group). The morphological changes in subcutaneous implantations were assessed after 1, 3, 5, 7, 10, 14, 21 days. The data were submitted to Friedman nonparametric test to analyze the comparisons among observation periods and to allow the comparison among groups. Results: Differences were found in the analysis of the inflammatory response when comparing the tested materials (p values <= 0.05). In test group was observed hemorrhage, edema and intense inflammatory infiltrate predominantly neutrophilic around material. From 3-day and subsequent periods was verified granulation tissue externally at this infiltrate. From 10-day on was observed crescent area of degradation of chlorhexidine chip, associated with neutrophilic and macrophagic infiltrate, that maintained until 21-day. In the control group, moderate inflammatory infiltrate was observed initially, predominantly polymorphonuclear, edema and granulation tissue 3-day period. The inflammatory infiltrate was gradually replaced for granulation tissue, culminating in a fibrous capsule. Giant multinucleate cells situated at contact interface with the coating was examined since 3-day and persisted until 21-day. Conclusion: The chlorhexidine chip induces an intense acute inflammatory response at subcutaneous tissue of rats. Therefore, at conditions of this study was not biocompatible.São Paulo State Univ, Sao Jose dos Campos Dent Sch, Dept Diag & Surg, UNESP,Biosci Ctr Special Hlth Care Needs CEBAPE, BR-12245000 Sao Jose Dos Campos, SP, BrazilSão Paulo State Univ, Sao Jose dos Campos Dent Sch, UNESP, Dept Dent Mat & Prosthesis, BR-12245000 Sao Jose Dos Campos, SP, BrazilSão Paulo State Univ, Sao Jose dos Campos Dent Sch, UNESP, Periodont Div,Dept Diag & Surg, BR-12245000 Sao Jose Dos Campos, SP, BrazilSão Paulo State Univ, Sao Jose dos Campos Dent Sch, Dept Diag & Surg, UNESP,Biosci Ctr Special Hlth Care Needs CEBAPE, BR-12245000 Sao Jose Dos Campos, SP, BrazilSão Paulo State Univ, Sao Jose dos Campos Dent Sch, UNESP, Dept Dent Mat & Prosthesis, BR-12245000 Sao Jose Dos Campos, SP, BrazilSão Paulo State Univ, Sao Jose dos Campos Dent Sch, UNESP, Periodont Div,Dept Diag & Surg, BR-12245000 Sao Jose Dos Campos, SP, BrazilMedicina Oral S LUniversidade Estadual Paulista (Unesp)Monteiro, Adriana-Socorro-Ferreira [UNESP]Macedo, Luis-Guilherme-Scavone [UNESP]Macedo, Nelson-Luiz [UNESP]Feitosa, Fernanda-AlvesToyoshima, Thiago [UNESP]2014-05-20T14:04:50Z2014-05-20T14:04:50Z2011-03-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleE278-E284http://dx.doi.org/10.4317/medoral.16.e278Medicina Oral Patologia Oral Y Cirugia Bucal. Valencia: Medicina Oral S L, v. 16, n. 2, p. E278-E284, 2011.1698-4447http://hdl.handle.net/11449/2273410.4317/medoral.16.e278WOS:000288682100027Web of Sciencereponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengMedicina Oral Patologia Oral y Cirugia Bucal0,841info:eu-repo/semantics/openAccess2021-10-22T19:03:41Zoai:repositorio.unesp.br:11449/22734Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462024-08-05T19:29:14.919404Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false
dc.title.none.fl_str_mv Biocompatibility of a chlorhexidine local delivery system in a subcutaneous mouse model
title Biocompatibility of a chlorhexidine local delivery system in a subcutaneous mouse model
spellingShingle Biocompatibility of a chlorhexidine local delivery system in a subcutaneous mouse model
Monteiro, Adriana-Socorro-Ferreira [UNESP]
Biocompatibility
chlorhexidine toxicity
drug delivery system
periodontitis
title_short Biocompatibility of a chlorhexidine local delivery system in a subcutaneous mouse model
title_full Biocompatibility of a chlorhexidine local delivery system in a subcutaneous mouse model
title_fullStr Biocompatibility of a chlorhexidine local delivery system in a subcutaneous mouse model
title_full_unstemmed Biocompatibility of a chlorhexidine local delivery system in a subcutaneous mouse model
title_sort Biocompatibility of a chlorhexidine local delivery system in a subcutaneous mouse model
author Monteiro, Adriana-Socorro-Ferreira [UNESP]
author_facet Monteiro, Adriana-Socorro-Ferreira [UNESP]
Macedo, Luis-Guilherme-Scavone [UNESP]
Macedo, Nelson-Luiz [UNESP]
Feitosa, Fernanda-Alves
Toyoshima, Thiago [UNESP]
author_role author
author2 Macedo, Luis-Guilherme-Scavone [UNESP]
Macedo, Nelson-Luiz [UNESP]
Feitosa, Fernanda-Alves
Toyoshima, Thiago [UNESP]
author2_role author
author
author
author
dc.contributor.none.fl_str_mv Universidade Estadual Paulista (Unesp)
dc.contributor.author.fl_str_mv Monteiro, Adriana-Socorro-Ferreira [UNESP]
Macedo, Luis-Guilherme-Scavone [UNESP]
Macedo, Nelson-Luiz [UNESP]
Feitosa, Fernanda-Alves
Toyoshima, Thiago [UNESP]
dc.subject.por.fl_str_mv Biocompatibility
chlorhexidine toxicity
drug delivery system
periodontitis
topic Biocompatibility
chlorhexidine toxicity
drug delivery system
periodontitis
description Objective: This study aimed evaluating histologically and histomorphometrically the response of the conjunctive tissue face to the implant of chlorhexidine chips in the subcutaneous tissues of rats. Study Design: In this research 35 male rats Wistar were used to analyze the biocompatibility and the degradation process of chlorhexidine chip. In each animal, it was made 2 incisions for subcutaneous implantation of chlorhexidine chip (test group) and a polytetrafluorethylene membrane (control group). The morphological changes in subcutaneous implantations were assessed after 1, 3, 5, 7, 10, 14, 21 days. The data were submitted to Friedman nonparametric test to analyze the comparisons among observation periods and to allow the comparison among groups. Results: Differences were found in the analysis of the inflammatory response when comparing the tested materials (p values <= 0.05). In test group was observed hemorrhage, edema and intense inflammatory infiltrate predominantly neutrophilic around material. From 3-day and subsequent periods was verified granulation tissue externally at this infiltrate. From 10-day on was observed crescent area of degradation of chlorhexidine chip, associated with neutrophilic and macrophagic infiltrate, that maintained until 21-day. In the control group, moderate inflammatory infiltrate was observed initially, predominantly polymorphonuclear, edema and granulation tissue 3-day period. The inflammatory infiltrate was gradually replaced for granulation tissue, culminating in a fibrous capsule. Giant multinucleate cells situated at contact interface with the coating was examined since 3-day and persisted until 21-day. Conclusion: The chlorhexidine chip induces an intense acute inflammatory response at subcutaneous tissue of rats. Therefore, at conditions of this study was not biocompatible.
publishDate 2011
dc.date.none.fl_str_mv 2011-03-01
2014-05-20T14:04:50Z
2014-05-20T14:04:50Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://dx.doi.org/10.4317/medoral.16.e278
Medicina Oral Patologia Oral Y Cirugia Bucal. Valencia: Medicina Oral S L, v. 16, n. 2, p. E278-E284, 2011.
1698-4447
http://hdl.handle.net/11449/22734
10.4317/medoral.16.e278
WOS:000288682100027
url http://dx.doi.org/10.4317/medoral.16.e278
http://hdl.handle.net/11449/22734
identifier_str_mv Medicina Oral Patologia Oral Y Cirugia Bucal. Valencia: Medicina Oral S L, v. 16, n. 2, p. E278-E284, 2011.
1698-4447
10.4317/medoral.16.e278
WOS:000288682100027
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv Medicina Oral Patologia Oral y Cirugia Bucal
0,841
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv E278-E284
dc.publisher.none.fl_str_mv Medicina Oral S L
publisher.none.fl_str_mv Medicina Oral S L
dc.source.none.fl_str_mv Web of Science
reponame:Repositório Institucional da UNESP
instname:Universidade Estadual Paulista (UNESP)
instacron:UNESP
instname_str Universidade Estadual Paulista (UNESP)
instacron_str UNESP
institution UNESP
reponame_str Repositório Institucional da UNESP
collection Repositório Institucional da UNESP
repository.name.fl_str_mv Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)
repository.mail.fl_str_mv
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