Síntese de nanopartículas de prata a partir da romã (Punica granatum): análises físico-química, antibacteriana e citotóxica

• Autor(a) principal: Jacometo, Willian Henrique [UNESP]
• Data de Publicação: 2017
• Tipo de documento: Trabalho de conclusão de curso
• Idioma: por
• Título da fonte: Repositório Institucional da UNESP
• Texto Completo: http://hdl.handle.net/11449/156877; http://www.athena.biblioteca.unesp.br/exlibris/bd/capelo/2018-07-04/000905333.pdf
• topic: Nanotecnologia; Biofilmes; Prata; Fibroblastos; Punicaceae; Nanotechnology

The aim of this study was to synthetize silver nanoparticles (NPAg) through extract of pomegranate peel extract (Punica granatum L.) and to evaluate its effect against Streptococcus mutans ((ATCC 25175) and the cytotoxicity in fibroblast cells. The silver nanoparticles were synthesized reducing the silver ions (Ag+) from silver nitrate (AgNO3) using the alcoholic extract from pomegranate peel obtained by maceration and percolation. The formation of silver nanoparticles was confirmed by x-Ray diffraction and scanning electron microcopy. Following, the minimum inhibitory concentration (MIC) of NPAg was calculated against S. mutans planktonic cells by the microdilution method. The effect of different concentrations and periods of exposition with NPAg on fibroblast cells of mice (L929) was evaluated calculating the percentage of viable cells using Alamar Blue colorimetric technique. In this assay, it was used as control NPAg produced using as reducing agents sodium citrate (NPAg/C) or sodium borohydrate (NPAg /B). All tests were performed in triplicate in three different occasions. NPAg were formed with regular and circular forms with average size of 50 nm. NPAg produced by peel extract of pomegranate were effective against S. mutans planktonic cells at 78.1 μg/mL and were significantly less cytotoxic than NPAg synthetized using conventional reducing agents. Indeed, green NPAg started to be toxic only from 125 μg/mL. So, it was concluded that the extract of pomegranate peel produced NPAg, and, besides these particles presented antibacterial effect against S. mutans they were expressively less cytotoxic than those NPAg synthetized through conventional chemical routes