In vivo effects of Bothrops jararaca venom on metabolic profile and on muscle protein metabolism in rats
Autor(a) principal: | |
---|---|
Data de Publicação: | 2008 |
Outros Autores: | , , , , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UNESP |
Texto Completo: | http://dx.doi.org/10.4269/ajtmh.2008.79.771 http://hdl.handle.net/11449/225328 |
Resumo: | This study investigated the in vivo effects of the Bothrops jararaca venom (BjV) on general metabolic profile and, specifically, on muscle protein metabolism in rats. The crude venom (0.4 mg/kg body weight, IV) was infused in awake rats, and plasma activity of enzymes and metabolites levels were determined after 1, 2, 3, and 4 hours. BjV increased urea, lactate, and activities of creatine kinase, lactate dehydrogenase, and aspartate aminotransferase after 4 hours. The content of liver glycogen was reduced by BjV. Protein metabolism was evaluated by means of microdialysis technique and in isolated muscles. BjV induced increase in the muscle interstitial-arterial tyrosine concentration difference, indicating a high protein catabolism. The myotoxicity induced by this venom is associated with reduction of protein synthesis and increase in rates of overall proteolysis, which was accompanied by activation of lysosomal and ubiquitin-proteasome systems without changes in protein levels of cathepsins and ubiquitin-protein conjugates. Copyright © 2008 by The American Society of Tropical Medicine and Hygiene. |
id |
UNSP_3215ca25575f1a09860b71232a86e801 |
---|---|
oai_identifier_str |
oai:repositorio.unesp.br:11449/225328 |
network_acronym_str |
UNSP |
network_name_str |
Repositório Institucional da UNESP |
repository_id_str |
2946 |
spelling |
In vivo effects of Bothrops jararaca venom on metabolic profile and on muscle protein metabolism in ratsThis study investigated the in vivo effects of the Bothrops jararaca venom (BjV) on general metabolic profile and, specifically, on muscle protein metabolism in rats. The crude venom (0.4 mg/kg body weight, IV) was infused in awake rats, and plasma activity of enzymes and metabolites levels were determined after 1, 2, 3, and 4 hours. BjV increased urea, lactate, and activities of creatine kinase, lactate dehydrogenase, and aspartate aminotransferase after 4 hours. The content of liver glycogen was reduced by BjV. Protein metabolism was evaluated by means of microdialysis technique and in isolated muscles. BjV induced increase in the muscle interstitial-arterial tyrosine concentration difference, indicating a high protein catabolism. The myotoxicity induced by this venom is associated with reduction of protein synthesis and increase in rates of overall proteolysis, which was accompanied by activation of lysosomal and ubiquitin-proteasome systems without changes in protein levels of cathepsins and ubiquitin-protein conjugates. Copyright © 2008 by The American Society of Tropical Medicine and Hygiene.Department of Physiology School of Medicine of Ribeirão Preto USP, 14049-900, Ribeirão Preto, SPDepartment of Ophthalmology Otorhinolaryngology and Head and Neck Surgery School of Medicine of Botucatu UNESP, 18618-000, Botucatu, SPDepartment of Molecular Biology Division of Nephrology School of Medicine of São José do Rio Preto, 15090-000, São José do Rio Preto, SPDepartment of Physiology and Biochemistry and Immunology School of Medicine of Ribeirão Preto USP, 14049-900, Ribeirão Preto, SPDepartment of Ophthalmology Otorhinolaryngology and Head and Neck Surgery School of Medicine of Botucatu UNESP, 18618-000, Botucatu, SPUniversidade de São Paulo (USP)Universidade Estadual Paulista (UNESP)School of Medicine of São José do Rio PretoGonçalves, Dawit A. P.Silva, Érico V. C. M. [UNESP]Graça, Flávia A.Lira, Eduardo C.Zanon, Neusa M.Mendes, Glória E.Burdmann, Emmanuel A.Migliorini, Renato H.Kettelhut, Isis C.Navegantes, Luiz C. C.2022-04-28T20:45:28Z2022-04-28T20:45:28Z2008-01-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article771-778http://dx.doi.org/10.4269/ajtmh.2008.79.771American Journal of Tropical Medicine and Hygiene, v. 79, n. 5, p. 771-778, 2008.0002-9637http://hdl.handle.net/11449/22532810.4269/ajtmh.2008.79.7712-s2.0-55849093962Scopusreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengAmerican Journal of Tropical Medicine and Hygieneinfo:eu-repo/semantics/openAccess2022-04-28T20:45:29Zoai:repositorio.unesp.br:11449/225328Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462022-04-28T20:45:29Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false |
dc.title.none.fl_str_mv |
In vivo effects of Bothrops jararaca venom on metabolic profile and on muscle protein metabolism in rats |
title |
In vivo effects of Bothrops jararaca venom on metabolic profile and on muscle protein metabolism in rats |
spellingShingle |
In vivo effects of Bothrops jararaca venom on metabolic profile and on muscle protein metabolism in rats Gonçalves, Dawit A. P. |
title_short |
In vivo effects of Bothrops jararaca venom on metabolic profile and on muscle protein metabolism in rats |
title_full |
In vivo effects of Bothrops jararaca venom on metabolic profile and on muscle protein metabolism in rats |
title_fullStr |
In vivo effects of Bothrops jararaca venom on metabolic profile and on muscle protein metabolism in rats |
title_full_unstemmed |
In vivo effects of Bothrops jararaca venom on metabolic profile and on muscle protein metabolism in rats |
title_sort |
In vivo effects of Bothrops jararaca venom on metabolic profile and on muscle protein metabolism in rats |
author |
Gonçalves, Dawit A. P. |
author_facet |
Gonçalves, Dawit A. P. Silva, Érico V. C. M. [UNESP] Graça, Flávia A. Lira, Eduardo C. Zanon, Neusa M. Mendes, Glória E. Burdmann, Emmanuel A. Migliorini, Renato H. Kettelhut, Isis C. Navegantes, Luiz C. C. |
author_role |
author |
author2 |
Silva, Érico V. C. M. [UNESP] Graça, Flávia A. Lira, Eduardo C. Zanon, Neusa M. Mendes, Glória E. Burdmann, Emmanuel A. Migliorini, Renato H. Kettelhut, Isis C. Navegantes, Luiz C. C. |
author2_role |
author author author author author author author author author |
dc.contributor.none.fl_str_mv |
Universidade de São Paulo (USP) Universidade Estadual Paulista (UNESP) School of Medicine of São José do Rio Preto |
dc.contributor.author.fl_str_mv |
Gonçalves, Dawit A. P. Silva, Érico V. C. M. [UNESP] Graça, Flávia A. Lira, Eduardo C. Zanon, Neusa M. Mendes, Glória E. Burdmann, Emmanuel A. Migliorini, Renato H. Kettelhut, Isis C. Navegantes, Luiz C. C. |
description |
This study investigated the in vivo effects of the Bothrops jararaca venom (BjV) on general metabolic profile and, specifically, on muscle protein metabolism in rats. The crude venom (0.4 mg/kg body weight, IV) was infused in awake rats, and plasma activity of enzymes and metabolites levels were determined after 1, 2, 3, and 4 hours. BjV increased urea, lactate, and activities of creatine kinase, lactate dehydrogenase, and aspartate aminotransferase after 4 hours. The content of liver glycogen was reduced by BjV. Protein metabolism was evaluated by means of microdialysis technique and in isolated muscles. BjV induced increase in the muscle interstitial-arterial tyrosine concentration difference, indicating a high protein catabolism. The myotoxicity induced by this venom is associated with reduction of protein synthesis and increase in rates of overall proteolysis, which was accompanied by activation of lysosomal and ubiquitin-proteasome systems without changes in protein levels of cathepsins and ubiquitin-protein conjugates. Copyright © 2008 by The American Society of Tropical Medicine and Hygiene. |
publishDate |
2008 |
dc.date.none.fl_str_mv |
2008-01-01 2022-04-28T20:45:28Z 2022-04-28T20:45:28Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://dx.doi.org/10.4269/ajtmh.2008.79.771 American Journal of Tropical Medicine and Hygiene, v. 79, n. 5, p. 771-778, 2008. 0002-9637 http://hdl.handle.net/11449/225328 10.4269/ajtmh.2008.79.771 2-s2.0-55849093962 |
url |
http://dx.doi.org/10.4269/ajtmh.2008.79.771 http://hdl.handle.net/11449/225328 |
identifier_str_mv |
American Journal of Tropical Medicine and Hygiene, v. 79, n. 5, p. 771-778, 2008. 0002-9637 10.4269/ajtmh.2008.79.771 2-s2.0-55849093962 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
American Journal of Tropical Medicine and Hygiene |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
771-778 |
dc.source.none.fl_str_mv |
Scopus reponame:Repositório Institucional da UNESP instname:Universidade Estadual Paulista (UNESP) instacron:UNESP |
instname_str |
Universidade Estadual Paulista (UNESP) |
instacron_str |
UNESP |
institution |
UNESP |
reponame_str |
Repositório Institucional da UNESP |
collection |
Repositório Institucional da UNESP |
repository.name.fl_str_mv |
Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP) |
repository.mail.fl_str_mv |
|
_version_ |
1799965174942138368 |