Involvement of pituitary gonadotropins, gonadal steroids and breeding season in sex change of protogynous dusky grouper, Epinephelus marginatus (Teleostei: Serranidae), induced by a non-steroidal aromatase inhibitor
Autor(a) principal: | |
---|---|
Data de Publicação: | 2013 |
Outros Autores: | , , , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UNESP |
Texto Completo: | http://dx.doi.org/10.1016/j.ygcen.2013.06.012 http://hdl.handle.net/11449/76738 |
Resumo: | Two experiments were performed using the aromatase inhibitor (AI) letrozole (100mg/kg) to promote sex change, from female-to-male, in protogynous dusky grouper. One experiment was performed during the breeding season (spring) and the other at the end of the breeding season (summer). During the spring, AI promoted sex change after 9weeks and the sperm produced was able to fertilize grouper oocytes. During the summer, the sex change was incomplete; intersex individuals were present and sperm was not released by any of the animals. Sex changed gonads had a lamellar architecture; cysts of spermatocytes and spermatozoa in the lumen of the germinal compartment. In the spring, after 4weeks, 11ketotestosterone (11KT) levels were higher in the AI than in control fish, and after 9weeks, coincident with semen release, testosterone levels increased in the AI group, while 11KT returned to the initial levels. Estradiol (E2) levels remained unchanged during the experimental period. Instead of decreasing throughout the period, as in control group, 17 α-OH progesterone levels did not change in the AI-treated fish, resulting in higher values after 9weeks when compared with control fish. fshβ and lhβ gene expression in the AI animals were lower compared with control fish after 9weeks. The use of AI was effective to obtain functional males during the breeding season. The increase in androgens, modulated by gonadotropins, triggered the sex change, enabling the development of male germ cells, whereas a decrease in E2 levels was not required to change sex in dusky grouper. © 2013 Elsevier Inc. |
id |
UNSP_388bdc58acae07f22f83d5e8026eb09d |
---|---|
oai_identifier_str |
oai:repositorio.unesp.br:11449/76738 |
network_acronym_str |
UNSP |
network_name_str |
Repositório Institucional da UNESP |
repository_id_str |
2946 |
spelling |
Involvement of pituitary gonadotropins, gonadal steroids and breeding season in sex change of protogynous dusky grouper, Epinephelus marginatus (Teleostei: Serranidae), induced by a non-steroidal aromatase inhibitorAromatase inhibitorArtificial sex changeFollicle-stimulating hormoneGonadal steroidsLetrozoleLuteinizing hormoneTwo experiments were performed using the aromatase inhibitor (AI) letrozole (100mg/kg) to promote sex change, from female-to-male, in protogynous dusky grouper. One experiment was performed during the breeding season (spring) and the other at the end of the breeding season (summer). During the spring, AI promoted sex change after 9weeks and the sperm produced was able to fertilize grouper oocytes. During the summer, the sex change was incomplete; intersex individuals were present and sperm was not released by any of the animals. Sex changed gonads had a lamellar architecture; cysts of spermatocytes and spermatozoa in the lumen of the germinal compartment. In the spring, after 4weeks, 11ketotestosterone (11KT) levels were higher in the AI than in control fish, and after 9weeks, coincident with semen release, testosterone levels increased in the AI group, while 11KT returned to the initial levels. Estradiol (E2) levels remained unchanged during the experimental period. Instead of decreasing throughout the period, as in control group, 17 α-OH progesterone levels did not change in the AI-treated fish, resulting in higher values after 9weeks when compared with control fish. fshβ and lhβ gene expression in the AI animals were lower compared with control fish after 9weeks. The use of AI was effective to obtain functional males during the breeding season. The increase in androgens, modulated by gonadotropins, triggered the sex change, enabling the development of male germ cells, whereas a decrease in E2 levels was not required to change sex in dusky grouper. © 2013 Elsevier Inc.Instituto de Biociências Universidade de São Paulo, Rua do Matão, Trav. 14, 321, São Paulo 05508-090, SPUniversidade Estadual Paulista Júlio de Mesquita Filho Centro de Aquicultura (CAUNESP), Via de Acesso Prof. Paulo Donato Castelane, s/n., Jaboticabal 14884-900, SPCentro de Biologia Marinha da Universidade de São Paulo (CEBIMar/USP) Rodovia Manoel Hypólito do Rego, Km. 131, 5, São Sebastião CEP 11600-000. SPUniversidade Estadual Paulista Júlio de Mesquita Filho Centro de Aquicultura (CAUNESP), Via de Acesso Prof. Paulo Donato Castelane, s/n., Jaboticabal 14884-900, SPUniversidade de São Paulo (USP)Universidade Estadual Paulista (Unesp)Garcia, Carlos Eduardo de O.Araújo, Bruno C.Mello, Paulo H.Narcizo, Amanda de M.Rodrigues-Filho, Jandyr A. [UNESP]Medrado, Andreone T.Zampieri, Ricardo A.Floeter-Winter, Lucile M.Moreira, Renata Guimarães2014-05-27T11:30:48Z2014-05-27T11:30:48Z2013-10-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article170-180application/pdfhttp://dx.doi.org/10.1016/j.ygcen.2013.06.012General and Comparative Endocrinology, v. 192, p. 170-180.0016-64801095-6840http://hdl.handle.net/11449/7673810.1016/j.ygcen.2013.06.012WOS:0003257364000202-s2.0-848844161422-s2.0-84884416142.pdfScopusreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengGeneral and Comparative Endocrinology2.5641,1011,101info:eu-repo/semantics/openAccess2024-04-09T15:43:44Zoai:repositorio.unesp.br:11449/76738Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462024-04-09T15:43:44Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false |
dc.title.none.fl_str_mv |
Involvement of pituitary gonadotropins, gonadal steroids and breeding season in sex change of protogynous dusky grouper, Epinephelus marginatus (Teleostei: Serranidae), induced by a non-steroidal aromatase inhibitor |
title |
Involvement of pituitary gonadotropins, gonadal steroids and breeding season in sex change of protogynous dusky grouper, Epinephelus marginatus (Teleostei: Serranidae), induced by a non-steroidal aromatase inhibitor |
spellingShingle |
Involvement of pituitary gonadotropins, gonadal steroids and breeding season in sex change of protogynous dusky grouper, Epinephelus marginatus (Teleostei: Serranidae), induced by a non-steroidal aromatase inhibitor Garcia, Carlos Eduardo de O. Aromatase inhibitor Artificial sex change Follicle-stimulating hormone Gonadal steroids Letrozole Luteinizing hormone |
title_short |
Involvement of pituitary gonadotropins, gonadal steroids and breeding season in sex change of protogynous dusky grouper, Epinephelus marginatus (Teleostei: Serranidae), induced by a non-steroidal aromatase inhibitor |
title_full |
Involvement of pituitary gonadotropins, gonadal steroids and breeding season in sex change of protogynous dusky grouper, Epinephelus marginatus (Teleostei: Serranidae), induced by a non-steroidal aromatase inhibitor |
title_fullStr |
Involvement of pituitary gonadotropins, gonadal steroids and breeding season in sex change of protogynous dusky grouper, Epinephelus marginatus (Teleostei: Serranidae), induced by a non-steroidal aromatase inhibitor |
title_full_unstemmed |
Involvement of pituitary gonadotropins, gonadal steroids and breeding season in sex change of protogynous dusky grouper, Epinephelus marginatus (Teleostei: Serranidae), induced by a non-steroidal aromatase inhibitor |
title_sort |
Involvement of pituitary gonadotropins, gonadal steroids and breeding season in sex change of protogynous dusky grouper, Epinephelus marginatus (Teleostei: Serranidae), induced by a non-steroidal aromatase inhibitor |
author |
Garcia, Carlos Eduardo de O. |
author_facet |
Garcia, Carlos Eduardo de O. Araújo, Bruno C. Mello, Paulo H. Narcizo, Amanda de M. Rodrigues-Filho, Jandyr A. [UNESP] Medrado, Andreone T. Zampieri, Ricardo A. Floeter-Winter, Lucile M. Moreira, Renata Guimarães |
author_role |
author |
author2 |
Araújo, Bruno C. Mello, Paulo H. Narcizo, Amanda de M. Rodrigues-Filho, Jandyr A. [UNESP] Medrado, Andreone T. Zampieri, Ricardo A. Floeter-Winter, Lucile M. Moreira, Renata Guimarães |
author2_role |
author author author author author author author author |
dc.contributor.none.fl_str_mv |
Universidade de São Paulo (USP) Universidade Estadual Paulista (Unesp) |
dc.contributor.author.fl_str_mv |
Garcia, Carlos Eduardo de O. Araújo, Bruno C. Mello, Paulo H. Narcizo, Amanda de M. Rodrigues-Filho, Jandyr A. [UNESP] Medrado, Andreone T. Zampieri, Ricardo A. Floeter-Winter, Lucile M. Moreira, Renata Guimarães |
dc.subject.por.fl_str_mv |
Aromatase inhibitor Artificial sex change Follicle-stimulating hormone Gonadal steroids Letrozole Luteinizing hormone |
topic |
Aromatase inhibitor Artificial sex change Follicle-stimulating hormone Gonadal steroids Letrozole Luteinizing hormone |
description |
Two experiments were performed using the aromatase inhibitor (AI) letrozole (100mg/kg) to promote sex change, from female-to-male, in protogynous dusky grouper. One experiment was performed during the breeding season (spring) and the other at the end of the breeding season (summer). During the spring, AI promoted sex change after 9weeks and the sperm produced was able to fertilize grouper oocytes. During the summer, the sex change was incomplete; intersex individuals were present and sperm was not released by any of the animals. Sex changed gonads had a lamellar architecture; cysts of spermatocytes and spermatozoa in the lumen of the germinal compartment. In the spring, after 4weeks, 11ketotestosterone (11KT) levels were higher in the AI than in control fish, and after 9weeks, coincident with semen release, testosterone levels increased in the AI group, while 11KT returned to the initial levels. Estradiol (E2) levels remained unchanged during the experimental period. Instead of decreasing throughout the period, as in control group, 17 α-OH progesterone levels did not change in the AI-treated fish, resulting in higher values after 9weeks when compared with control fish. fshβ and lhβ gene expression in the AI animals were lower compared with control fish after 9weeks. The use of AI was effective to obtain functional males during the breeding season. The increase in androgens, modulated by gonadotropins, triggered the sex change, enabling the development of male germ cells, whereas a decrease in E2 levels was not required to change sex in dusky grouper. © 2013 Elsevier Inc. |
publishDate |
2013 |
dc.date.none.fl_str_mv |
2013-10-01 2014-05-27T11:30:48Z 2014-05-27T11:30:48Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://dx.doi.org/10.1016/j.ygcen.2013.06.012 General and Comparative Endocrinology, v. 192, p. 170-180. 0016-6480 1095-6840 http://hdl.handle.net/11449/76738 10.1016/j.ygcen.2013.06.012 WOS:000325736400020 2-s2.0-84884416142 2-s2.0-84884416142.pdf |
url |
http://dx.doi.org/10.1016/j.ygcen.2013.06.012 http://hdl.handle.net/11449/76738 |
identifier_str_mv |
General and Comparative Endocrinology, v. 192, p. 170-180. 0016-6480 1095-6840 10.1016/j.ygcen.2013.06.012 WOS:000325736400020 2-s2.0-84884416142 2-s2.0-84884416142.pdf |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
General and Comparative Endocrinology 2.564 1,101 1,101 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
170-180 application/pdf |
dc.source.none.fl_str_mv |
Scopus reponame:Repositório Institucional da UNESP instname:Universidade Estadual Paulista (UNESP) instacron:UNESP |
instname_str |
Universidade Estadual Paulista (UNESP) |
instacron_str |
UNESP |
institution |
UNESP |
reponame_str |
Repositório Institucional da UNESP |
collection |
Repositório Institucional da UNESP |
repository.name.fl_str_mv |
Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP) |
repository.mail.fl_str_mv |
|
_version_ |
1803047482559561728 |