Evaluation of antimicrobial photodynamic therapy with acidic methylene blue for the treatment of experimental periodontitis
Autor(a) principal: | |
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Data de Publicação: | 2022 |
Outros Autores: | , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UNESP |
Texto Completo: | http://dx.doi.org/10.1371/journal.pone.0263103 http://hdl.handle.net/11449/234128 |
Resumo: | Objective To investigate the security and effectiveness of antimicrobial photodynamic therapy (aPDT) with a citric acid-based methylene blue (MB) on the periodontal repair following the treatment of ligature-induced experimental periodontitis (EP) in rats. Material and methods Were used 120 male rats, randomly divided into 4 experimental groups (n = 30): no treatment (NT), SRP alone (SRP), SRP plus aPDT using conventional MB pH 7.0 (aPDT-pH7), SRP plus aPDT using acidic MB pH 1.0 (aPDT-pH1). EP was induced at day 0 by the placement of a ligature around the mandibular left first molars. Ten animals per group/period were euthanized at 14, 22 and 37 days. Histopathological, histometric (percentage of bone in the furcation [PBF]) and immunohistochemical (for tartrate-resistant acid phosphatase [TRAP] and osteocalcin [OCN]) analyses were performed. Data were statistically analyzed. Results aPDT-pH1 showed the highest PBF as compared with the other treatments. Collectively, tissues’ reaction to both dyes were controlled and healthy for the periodontium. Both aPDT protocols reduced the extent and intensity of the local inflammatory response, reduced the alveolar bone resorption, and promoted a better structural arrangement of the connective tissue as compared with SRP. TRAP expression was downregulated while OCN expression was upregulated by aPDT as compared with SRP alone. Conclusion Our data implicate that the novel MB pH 1.0 is as safe as the conventional MB for use in aPDT and raises its additional benefit of increasing the amount of alveolar bone in the furcation. |
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Repositório Institucional da UNESP |
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Evaluation of antimicrobial photodynamic therapy with acidic methylene blue for the treatment of experimental periodontitisObjective To investigate the security and effectiveness of antimicrobial photodynamic therapy (aPDT) with a citric acid-based methylene blue (MB) on the periodontal repair following the treatment of ligature-induced experimental periodontitis (EP) in rats. Material and methods Were used 120 male rats, randomly divided into 4 experimental groups (n = 30): no treatment (NT), SRP alone (SRP), SRP plus aPDT using conventional MB pH 7.0 (aPDT-pH7), SRP plus aPDT using acidic MB pH 1.0 (aPDT-pH1). EP was induced at day 0 by the placement of a ligature around the mandibular left first molars. Ten animals per group/period were euthanized at 14, 22 and 37 days. Histopathological, histometric (percentage of bone in the furcation [PBF]) and immunohistochemical (for tartrate-resistant acid phosphatase [TRAP] and osteocalcin [OCN]) analyses were performed. Data were statistically analyzed. Results aPDT-pH1 showed the highest PBF as compared with the other treatments. Collectively, tissues’ reaction to both dyes were controlled and healthy for the periodontium. Both aPDT protocols reduced the extent and intensity of the local inflammatory response, reduced the alveolar bone resorption, and promoted a better structural arrangement of the connective tissue as compared with SRP. TRAP expression was downregulated while OCN expression was upregulated by aPDT as compared with SRP alone. Conclusion Our data implicate that the novel MB pH 1.0 is as safe as the conventional MB for use in aPDT and raises its additional benefit of increasing the amount of alveolar bone in the furcation.Periodontics Division Department of Diagnosis and Surgery School of Dentistry São Paulo State University (Unesp)School of Dentistry Nucleus of Study and Research in Periodontics and Implantology (NEPPI) São Paulo State University (Unesp), SPDepartment of Basic Science School of Dentistry São Paulo State University (Unesp)Periodontics Division Department of Diagnosis and Surgery School of Dentistry São Paulo State University (Unesp)School of Dentistry Nucleus of Study and Research in Periodontics and Implantology (NEPPI) São Paulo State University (Unesp), SPDepartment of Basic Science School of Dentistry São Paulo State University (Unesp)Universidade Estadual Paulista (UNESP)de Almeida, Juliano Milanezi [UNESP]Matheus, Henrique Rinaldi [UNESP]Alves, Breno Edson Sendão [UNESP]Gusman, David Jonathan Rodrigues [UNESP]Nagata, Maria José Hitomi [UNESP]de Abreu Furquim, Elisa Mara [UNESP]Ervolino, Edilson [UNESP]2022-05-01T13:41:33Z2022-05-01T13:41:33Z2022-02-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articlehttp://dx.doi.org/10.1371/journal.pone.0263103PLoS ONE, v. 17, n. 2 February, 2022.1932-6203http://hdl.handle.net/11449/23412810.1371/journal.pone.02631032-s2.0-85124385523Scopusreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengPLoS ONEinfo:eu-repo/semantics/openAccess2022-05-01T13:41:33Zoai:repositorio.unesp.br:11449/234128Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462024-08-05T22:22:54.941724Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false |
dc.title.none.fl_str_mv |
Evaluation of antimicrobial photodynamic therapy with acidic methylene blue for the treatment of experimental periodontitis |
title |
Evaluation of antimicrobial photodynamic therapy with acidic methylene blue for the treatment of experimental periodontitis |
spellingShingle |
Evaluation of antimicrobial photodynamic therapy with acidic methylene blue for the treatment of experimental periodontitis de Almeida, Juliano Milanezi [UNESP] |
title_short |
Evaluation of antimicrobial photodynamic therapy with acidic methylene blue for the treatment of experimental periodontitis |
title_full |
Evaluation of antimicrobial photodynamic therapy with acidic methylene blue for the treatment of experimental periodontitis |
title_fullStr |
Evaluation of antimicrobial photodynamic therapy with acidic methylene blue for the treatment of experimental periodontitis |
title_full_unstemmed |
Evaluation of antimicrobial photodynamic therapy with acidic methylene blue for the treatment of experimental periodontitis |
title_sort |
Evaluation of antimicrobial photodynamic therapy with acidic methylene blue for the treatment of experimental periodontitis |
author |
de Almeida, Juliano Milanezi [UNESP] |
author_facet |
de Almeida, Juliano Milanezi [UNESP] Matheus, Henrique Rinaldi [UNESP] Alves, Breno Edson Sendão [UNESP] Gusman, David Jonathan Rodrigues [UNESP] Nagata, Maria José Hitomi [UNESP] de Abreu Furquim, Elisa Mara [UNESP] Ervolino, Edilson [UNESP] |
author_role |
author |
author2 |
Matheus, Henrique Rinaldi [UNESP] Alves, Breno Edson Sendão [UNESP] Gusman, David Jonathan Rodrigues [UNESP] Nagata, Maria José Hitomi [UNESP] de Abreu Furquim, Elisa Mara [UNESP] Ervolino, Edilson [UNESP] |
author2_role |
author author author author author author |
dc.contributor.none.fl_str_mv |
Universidade Estadual Paulista (UNESP) |
dc.contributor.author.fl_str_mv |
de Almeida, Juliano Milanezi [UNESP] Matheus, Henrique Rinaldi [UNESP] Alves, Breno Edson Sendão [UNESP] Gusman, David Jonathan Rodrigues [UNESP] Nagata, Maria José Hitomi [UNESP] de Abreu Furquim, Elisa Mara [UNESP] Ervolino, Edilson [UNESP] |
description |
Objective To investigate the security and effectiveness of antimicrobial photodynamic therapy (aPDT) with a citric acid-based methylene blue (MB) on the periodontal repair following the treatment of ligature-induced experimental periodontitis (EP) in rats. Material and methods Were used 120 male rats, randomly divided into 4 experimental groups (n = 30): no treatment (NT), SRP alone (SRP), SRP plus aPDT using conventional MB pH 7.0 (aPDT-pH7), SRP plus aPDT using acidic MB pH 1.0 (aPDT-pH1). EP was induced at day 0 by the placement of a ligature around the mandibular left first molars. Ten animals per group/period were euthanized at 14, 22 and 37 days. Histopathological, histometric (percentage of bone in the furcation [PBF]) and immunohistochemical (for tartrate-resistant acid phosphatase [TRAP] and osteocalcin [OCN]) analyses were performed. Data were statistically analyzed. Results aPDT-pH1 showed the highest PBF as compared with the other treatments. Collectively, tissues’ reaction to both dyes were controlled and healthy for the periodontium. Both aPDT protocols reduced the extent and intensity of the local inflammatory response, reduced the alveolar bone resorption, and promoted a better structural arrangement of the connective tissue as compared with SRP. TRAP expression was downregulated while OCN expression was upregulated by aPDT as compared with SRP alone. Conclusion Our data implicate that the novel MB pH 1.0 is as safe as the conventional MB for use in aPDT and raises its additional benefit of increasing the amount of alveolar bone in the furcation. |
publishDate |
2022 |
dc.date.none.fl_str_mv |
2022-05-01T13:41:33Z 2022-05-01T13:41:33Z 2022-02-01 |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://dx.doi.org/10.1371/journal.pone.0263103 PLoS ONE, v. 17, n. 2 February, 2022. 1932-6203 http://hdl.handle.net/11449/234128 10.1371/journal.pone.0263103 2-s2.0-85124385523 |
url |
http://dx.doi.org/10.1371/journal.pone.0263103 http://hdl.handle.net/11449/234128 |
identifier_str_mv |
PLoS ONE, v. 17, n. 2 February, 2022. 1932-6203 10.1371/journal.pone.0263103 2-s2.0-85124385523 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
PLoS ONE |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.source.none.fl_str_mv |
Scopus reponame:Repositório Institucional da UNESP instname:Universidade Estadual Paulista (UNESP) instacron:UNESP |
instname_str |
Universidade Estadual Paulista (UNESP) |
instacron_str |
UNESP |
institution |
UNESP |
reponame_str |
Repositório Institucional da UNESP |
collection |
Repositório Institucional da UNESP |
repository.name.fl_str_mv |
Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP) |
repository.mail.fl_str_mv |
|
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1808129421796704256 |