Biocompatibility and bioactive potential of an experimental tricalcium silicate-based cement in comparison with Bio-C repair and MTA Repair HP materials
Autor(a) principal: | |
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Data de Publicação: | 2023 |
Outros Autores: | , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UNESP |
Texto Completo: | http://dx.doi.org/10.1111/iej.13863 http://hdl.handle.net/11449/249382 |
Resumo: | Aim: To evaluate the tissue reaction of a tricalcium silicate-based repair material associated with 30% calcium tungstate (TCS + CaWO4) in comparison to Bio-C Repair (Bio-C; Angelus) and to MTA Repair HP (MTA HP; Angelus). Methodology: Polyethylene tubes filled with one of the materials or left empty (control group, CG) were implanted into the subcutaneous tissues of rats for 7, 15, 30 and 60 days (n = 32/group). The capsule thickness, number of inflammatory cells, collagen content, interleukin-6 (IL-6), osteocalcin (OCN), von Kossa reaction and analysis under polarized light were evaluated. The data were subjected to generalized linear models for repeated measures, except the OCN. OCN data were submitted to Kruskal–Wallis and Dunn's post hoc test and Friedman followed by Nemenyi's test at significance level of 5%. Results: At all time points, significant differences in the number of inflammatory cells were not observed between TCS + CaWO4 and Bio-C, whereas, at 15, 30 and 60 days, no significant difference was detected between TCS + CaWO4 and MTA HP. At all periods, significant differences were not detected in the number of fibroblasts in TCS + CaWO4 versus MTA HP, and, at 60 days, no significant difference was demonstrated between these groups and CG. Significant differences in the immunoexpression of IL-6 were not detected amongst bioceramic materials at all periods. From 7 to 60 days, significant reduction in the number of inflammatory cells, number of IL-6-immunopositive cells and in the capsule thickness was accompanied by significant increase in the collagen in all groups. OCN-immunolabelled cells, von Kossa-positive structures and amorphous calcite deposits were observed around all materials, whereas, in the CG, these structures were not seen. Conclusions: These findings indicate that the experimental material (TCS + CaWO4) is biocompatible and has a bioactive potential, similar to the MTA HP and Bio-C Repair, and suggest its use as a root repair material. |
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Biocompatibility and bioactive potential of an experimental tricalcium silicate-based cement in comparison with Bio-C repair and MTA Repair HP materialsanimal modelbioceramic materialsinterleukin-6osteocalcinrepair materialstissue reactionAim: To evaluate the tissue reaction of a tricalcium silicate-based repair material associated with 30% calcium tungstate (TCS + CaWO4) in comparison to Bio-C Repair (Bio-C; Angelus) and to MTA Repair HP (MTA HP; Angelus). Methodology: Polyethylene tubes filled with one of the materials or left empty (control group, CG) were implanted into the subcutaneous tissues of rats for 7, 15, 30 and 60 days (n = 32/group). The capsule thickness, number of inflammatory cells, collagen content, interleukin-6 (IL-6), osteocalcin (OCN), von Kossa reaction and analysis under polarized light were evaluated. The data were subjected to generalized linear models for repeated measures, except the OCN. OCN data were submitted to Kruskal–Wallis and Dunn's post hoc test and Friedman followed by Nemenyi's test at significance level of 5%. Results: At all time points, significant differences in the number of inflammatory cells were not observed between TCS + CaWO4 and Bio-C, whereas, at 15, 30 and 60 days, no significant difference was detected between TCS + CaWO4 and MTA HP. At all periods, significant differences were not detected in the number of fibroblasts in TCS + CaWO4 versus MTA HP, and, at 60 days, no significant difference was demonstrated between these groups and CG. Significant differences in the immunoexpression of IL-6 were not detected amongst bioceramic materials at all periods. From 7 to 60 days, significant reduction in the number of inflammatory cells, number of IL-6-immunopositive cells and in the capsule thickness was accompanied by significant increase in the collagen in all groups. OCN-immunolabelled cells, von Kossa-positive structures and amorphous calcite deposits were observed around all materials, whereas, in the CG, these structures were not seen. Conclusions: These findings indicate that the experimental material (TCS + CaWO4) is biocompatible and has a bioactive potential, similar to the MTA HP and Bio-C Repair, and suggest its use as a root repair material.Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)Department of Restorative Dentistry São Paulo State University (UNESP)Laboratory of Histology and Embryology Department of Morphology Genetics Orthodontics and Pediatric Dentistry Dental School São Paulo State University (UNESP)Department of Restorative Dentistry São Paulo State University (UNESP)Laboratory of Histology and Embryology Department of Morphology Genetics Orthodontics and Pediatric Dentistry Dental School São Paulo State University (UNESP)CAPES: 001FAPESP: 2019/17739-5Universidade Estadual Paulista (UNESP)Queiroz, Marcela Borsatto [UNESP]Inada, Rafaela N. H. [UNESP]Jampani, José Leandro de Abreu [UNESP]Guerreiro-Tanomaru, Juliane Maria [UNESP]Sasso-Cerri, Estela [UNESP]Tanomaru-Filho, Mário [UNESP]Cerri, Paulo Sérgio [UNESP]2023-07-29T15:14:33Z2023-07-29T15:14:33Z2023-02-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article259-277http://dx.doi.org/10.1111/iej.13863International Endodontic Journal, v. 56, n. 2, p. 259-277, 2023.1365-25910143-2885http://hdl.handle.net/11449/24938210.1111/iej.138632-s2.0-85142095329Scopusreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengInternational Endodontic Journalinfo:eu-repo/semantics/openAccess2023-07-29T15:14:33Zoai:repositorio.unesp.br:11449/249382Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462024-08-05T16:36:39.419500Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false |
dc.title.none.fl_str_mv |
Biocompatibility and bioactive potential of an experimental tricalcium silicate-based cement in comparison with Bio-C repair and MTA Repair HP materials |
title |
Biocompatibility and bioactive potential of an experimental tricalcium silicate-based cement in comparison with Bio-C repair and MTA Repair HP materials |
spellingShingle |
Biocompatibility and bioactive potential of an experimental tricalcium silicate-based cement in comparison with Bio-C repair and MTA Repair HP materials Queiroz, Marcela Borsatto [UNESP] animal model bioceramic materials interleukin-6 osteocalcin repair materials tissue reaction |
title_short |
Biocompatibility and bioactive potential of an experimental tricalcium silicate-based cement in comparison with Bio-C repair and MTA Repair HP materials |
title_full |
Biocompatibility and bioactive potential of an experimental tricalcium silicate-based cement in comparison with Bio-C repair and MTA Repair HP materials |
title_fullStr |
Biocompatibility and bioactive potential of an experimental tricalcium silicate-based cement in comparison with Bio-C repair and MTA Repair HP materials |
title_full_unstemmed |
Biocompatibility and bioactive potential of an experimental tricalcium silicate-based cement in comparison with Bio-C repair and MTA Repair HP materials |
title_sort |
Biocompatibility and bioactive potential of an experimental tricalcium silicate-based cement in comparison with Bio-C repair and MTA Repair HP materials |
author |
Queiroz, Marcela Borsatto [UNESP] |
author_facet |
Queiroz, Marcela Borsatto [UNESP] Inada, Rafaela N. H. [UNESP] Jampani, José Leandro de Abreu [UNESP] Guerreiro-Tanomaru, Juliane Maria [UNESP] Sasso-Cerri, Estela [UNESP] Tanomaru-Filho, Mário [UNESP] Cerri, Paulo Sérgio [UNESP] |
author_role |
author |
author2 |
Inada, Rafaela N. H. [UNESP] Jampani, José Leandro de Abreu [UNESP] Guerreiro-Tanomaru, Juliane Maria [UNESP] Sasso-Cerri, Estela [UNESP] Tanomaru-Filho, Mário [UNESP] Cerri, Paulo Sérgio [UNESP] |
author2_role |
author author author author author author |
dc.contributor.none.fl_str_mv |
Universidade Estadual Paulista (UNESP) |
dc.contributor.author.fl_str_mv |
Queiroz, Marcela Borsatto [UNESP] Inada, Rafaela N. H. [UNESP] Jampani, José Leandro de Abreu [UNESP] Guerreiro-Tanomaru, Juliane Maria [UNESP] Sasso-Cerri, Estela [UNESP] Tanomaru-Filho, Mário [UNESP] Cerri, Paulo Sérgio [UNESP] |
dc.subject.por.fl_str_mv |
animal model bioceramic materials interleukin-6 osteocalcin repair materials tissue reaction |
topic |
animal model bioceramic materials interleukin-6 osteocalcin repair materials tissue reaction |
description |
Aim: To evaluate the tissue reaction of a tricalcium silicate-based repair material associated with 30% calcium tungstate (TCS + CaWO4) in comparison to Bio-C Repair (Bio-C; Angelus) and to MTA Repair HP (MTA HP; Angelus). Methodology: Polyethylene tubes filled with one of the materials or left empty (control group, CG) were implanted into the subcutaneous tissues of rats for 7, 15, 30 and 60 days (n = 32/group). The capsule thickness, number of inflammatory cells, collagen content, interleukin-6 (IL-6), osteocalcin (OCN), von Kossa reaction and analysis under polarized light were evaluated. The data were subjected to generalized linear models for repeated measures, except the OCN. OCN data were submitted to Kruskal–Wallis and Dunn's post hoc test and Friedman followed by Nemenyi's test at significance level of 5%. Results: At all time points, significant differences in the number of inflammatory cells were not observed between TCS + CaWO4 and Bio-C, whereas, at 15, 30 and 60 days, no significant difference was detected between TCS + CaWO4 and MTA HP. At all periods, significant differences were not detected in the number of fibroblasts in TCS + CaWO4 versus MTA HP, and, at 60 days, no significant difference was demonstrated between these groups and CG. Significant differences in the immunoexpression of IL-6 were not detected amongst bioceramic materials at all periods. From 7 to 60 days, significant reduction in the number of inflammatory cells, number of IL-6-immunopositive cells and in the capsule thickness was accompanied by significant increase in the collagen in all groups. OCN-immunolabelled cells, von Kossa-positive structures and amorphous calcite deposits were observed around all materials, whereas, in the CG, these structures were not seen. Conclusions: These findings indicate that the experimental material (TCS + CaWO4) is biocompatible and has a bioactive potential, similar to the MTA HP and Bio-C Repair, and suggest its use as a root repair material. |
publishDate |
2023 |
dc.date.none.fl_str_mv |
2023-07-29T15:14:33Z 2023-07-29T15:14:33Z 2023-02-01 |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://dx.doi.org/10.1111/iej.13863 International Endodontic Journal, v. 56, n. 2, p. 259-277, 2023. 1365-2591 0143-2885 http://hdl.handle.net/11449/249382 10.1111/iej.13863 2-s2.0-85142095329 |
url |
http://dx.doi.org/10.1111/iej.13863 http://hdl.handle.net/11449/249382 |
identifier_str_mv |
International Endodontic Journal, v. 56, n. 2, p. 259-277, 2023. 1365-2591 0143-2885 10.1111/iej.13863 2-s2.0-85142095329 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
International Endodontic Journal |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
259-277 |
dc.source.none.fl_str_mv |
Scopus reponame:Repositório Institucional da UNESP instname:Universidade Estadual Paulista (UNESP) instacron:UNESP |
instname_str |
Universidade Estadual Paulista (UNESP) |
instacron_str |
UNESP |
institution |
UNESP |
reponame_str |
Repositório Institucional da UNESP |
collection |
Repositório Institucional da UNESP |
repository.name.fl_str_mv |
Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP) |
repository.mail.fl_str_mv |
|
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1808128677370658816 |