The use of menthol in skin wound healing—anti-inflammatory potential, antioxidant defense system stimulation and increased epithelialization
Autor(a) principal: | |
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Data de Publicação: | 2021 |
Outros Autores: | , , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UNESP |
Texto Completo: | http://dx.doi.org/10.3390/pharmaceutics13111902 http://hdl.handle.net/11449/229930 |
Resumo: | Wound healing involves inflammatory, proliferative, and remodeling phases, in which various cells and chemical intermediates are involved. This study aimed to investigate the skin wound healing potential of menthol, as well as the mechanisms involved in its effect, after 3, 7, or 14 days of treatment, according to the phases of wound healing. Skin wound was performed in the back of Wistar rats, which were topically treated with vehicle cream; collagenase-based cream (1.2 U/g); or menthol-based cream at 0.25%, 0.5%, or 1.0% over 3, 7, or 14 days. Menthol cream at 0.5% accelerated the healing right from the inflammatory phase (3 days) by decreasing mRNA expression of inflammatory cytokines TNF-α and Il-6. At the proliferative phase (7 days), menthol 0.5% increased the activity of antioxidant enzymes SOD, GR, and GPx, as well as the level of GSH, in addition to decreasing the levels of inflammatory cytokines TNF-α, IL-6, and IL-1β and augmenting mRNA expression for Ki-67, a marker of cellular proliferation. At the remodeling phase (14 days), levels of inflammatory cytokines were decreased, and the level of Il-10 and its mRNA expression were increased in the menthol 0.5% group. Menthol presented skin wound healing activity by modulating the antioxidant system of the cells and the inflammatory response, in addition to stimulating epithelialization. |
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The use of menthol in skin wound healing—anti-inflammatory potential, antioxidant defense system stimulation and increased epithelializationAntioxidantGene expressionGSHIL-10InflammationMentholSkin wound healingTNF-αWound healing involves inflammatory, proliferative, and remodeling phases, in which various cells and chemical intermediates are involved. This study aimed to investigate the skin wound healing potential of menthol, as well as the mechanisms involved in its effect, after 3, 7, or 14 days of treatment, according to the phases of wound healing. Skin wound was performed in the back of Wistar rats, which were topically treated with vehicle cream; collagenase-based cream (1.2 U/g); or menthol-based cream at 0.25%, 0.5%, or 1.0% over 3, 7, or 14 days. Menthol cream at 0.5% accelerated the healing right from the inflammatory phase (3 days) by decreasing mRNA expression of inflammatory cytokines TNF-α and Il-6. At the proliferative phase (7 days), menthol 0.5% increased the activity of antioxidant enzymes SOD, GR, and GPx, as well as the level of GSH, in addition to decreasing the levels of inflammatory cytokines TNF-α, IL-6, and IL-1β and augmenting mRNA expression for Ki-67, a marker of cellular proliferation. At the remodeling phase (14 days), levels of inflammatory cytokines were decreased, and the level of Il-10 and its mRNA expression were increased in the menthol 0.5% group. Menthol presented skin wound healing activity by modulating the antioxidant system of the cells and the inflammatory response, in addition to stimulating epithelialization.Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)Department of Structural and Functional Biology Institute of Biosciences São Paulo State University (UNESP), Dr. Antonio Celso W Zanin Street, 250Department of Surgery and Veterinary Anesthesiology School of Veterinary Medicine and Animal Science São Paulo State University (UNESP), Dr. Walter M Correa StreetDepartment of Structural and Functional Biology Institute of Biosciences São Paulo State University (UNESP), Dr. Antonio Celso W Zanin Street, 250Department of Surgery and Veterinary Anesthesiology School of Veterinary Medicine and Animal Science São Paulo State University (UNESP), Dr. Walter M Correa StreetFAPESP: 2013/22202-4FAPESP: 2013/23340-1FAPESP: 2015/06636-0FAPESP: 2016/13307-5CNPq: 406854/2016-5Universidade Estadual Paulista (UNESP)Rozza, Ariane Leite [UNESP]Beserra, Fernando Pereira [UNESP]Vieira, Ana Júlia [UNESP]de Souza, Eduardo Oliveira [UNESP]Hussni, Carlos Alberto [UNESP]Martinez, Emanuel Ricardo Monteiro [UNESP]Nóbrega, Rafael Henrique [UNESP]Pellizzon, Cláudia Helena [UNESP]2022-04-29T08:36:42Z2022-04-29T08:36:42Z2021-11-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articlehttp://dx.doi.org/10.3390/pharmaceutics13111902Pharmaceutics, v. 13, n. 11, 2021.1999-4923http://hdl.handle.net/11449/22993010.3390/pharmaceutics131119022-s2.0-85119609400Scopusreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengPharmaceuticsinfo:eu-repo/semantics/openAccess2022-04-29T08:36:42Zoai:repositorio.unesp.br:11449/229930Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462024-08-05T21:16:39.981818Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false |
dc.title.none.fl_str_mv |
The use of menthol in skin wound healing—anti-inflammatory potential, antioxidant defense system stimulation and increased epithelialization |
title |
The use of menthol in skin wound healing—anti-inflammatory potential, antioxidant defense system stimulation and increased epithelialization |
spellingShingle |
The use of menthol in skin wound healing—anti-inflammatory potential, antioxidant defense system stimulation and increased epithelialization Rozza, Ariane Leite [UNESP] Antioxidant Gene expression GSH IL-10 Inflammation Menthol Skin wound healing TNF-α |
title_short |
The use of menthol in skin wound healing—anti-inflammatory potential, antioxidant defense system stimulation and increased epithelialization |
title_full |
The use of menthol in skin wound healing—anti-inflammatory potential, antioxidant defense system stimulation and increased epithelialization |
title_fullStr |
The use of menthol in skin wound healing—anti-inflammatory potential, antioxidant defense system stimulation and increased epithelialization |
title_full_unstemmed |
The use of menthol in skin wound healing—anti-inflammatory potential, antioxidant defense system stimulation and increased epithelialization |
title_sort |
The use of menthol in skin wound healing—anti-inflammatory potential, antioxidant defense system stimulation and increased epithelialization |
author |
Rozza, Ariane Leite [UNESP] |
author_facet |
Rozza, Ariane Leite [UNESP] Beserra, Fernando Pereira [UNESP] Vieira, Ana Júlia [UNESP] de Souza, Eduardo Oliveira [UNESP] Hussni, Carlos Alberto [UNESP] Martinez, Emanuel Ricardo Monteiro [UNESP] Nóbrega, Rafael Henrique [UNESP] Pellizzon, Cláudia Helena [UNESP] |
author_role |
author |
author2 |
Beserra, Fernando Pereira [UNESP] Vieira, Ana Júlia [UNESP] de Souza, Eduardo Oliveira [UNESP] Hussni, Carlos Alberto [UNESP] Martinez, Emanuel Ricardo Monteiro [UNESP] Nóbrega, Rafael Henrique [UNESP] Pellizzon, Cláudia Helena [UNESP] |
author2_role |
author author author author author author author |
dc.contributor.none.fl_str_mv |
Universidade Estadual Paulista (UNESP) |
dc.contributor.author.fl_str_mv |
Rozza, Ariane Leite [UNESP] Beserra, Fernando Pereira [UNESP] Vieira, Ana Júlia [UNESP] de Souza, Eduardo Oliveira [UNESP] Hussni, Carlos Alberto [UNESP] Martinez, Emanuel Ricardo Monteiro [UNESP] Nóbrega, Rafael Henrique [UNESP] Pellizzon, Cláudia Helena [UNESP] |
dc.subject.por.fl_str_mv |
Antioxidant Gene expression GSH IL-10 Inflammation Menthol Skin wound healing TNF-α |
topic |
Antioxidant Gene expression GSH IL-10 Inflammation Menthol Skin wound healing TNF-α |
description |
Wound healing involves inflammatory, proliferative, and remodeling phases, in which various cells and chemical intermediates are involved. This study aimed to investigate the skin wound healing potential of menthol, as well as the mechanisms involved in its effect, after 3, 7, or 14 days of treatment, according to the phases of wound healing. Skin wound was performed in the back of Wistar rats, which were topically treated with vehicle cream; collagenase-based cream (1.2 U/g); or menthol-based cream at 0.25%, 0.5%, or 1.0% over 3, 7, or 14 days. Menthol cream at 0.5% accelerated the healing right from the inflammatory phase (3 days) by decreasing mRNA expression of inflammatory cytokines TNF-α and Il-6. At the proliferative phase (7 days), menthol 0.5% increased the activity of antioxidant enzymes SOD, GR, and GPx, as well as the level of GSH, in addition to decreasing the levels of inflammatory cytokines TNF-α, IL-6, and IL-1β and augmenting mRNA expression for Ki-67, a marker of cellular proliferation. At the remodeling phase (14 days), levels of inflammatory cytokines were decreased, and the level of Il-10 and its mRNA expression were increased in the menthol 0.5% group. Menthol presented skin wound healing activity by modulating the antioxidant system of the cells and the inflammatory response, in addition to stimulating epithelialization. |
publishDate |
2021 |
dc.date.none.fl_str_mv |
2021-11-01 2022-04-29T08:36:42Z 2022-04-29T08:36:42Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://dx.doi.org/10.3390/pharmaceutics13111902 Pharmaceutics, v. 13, n. 11, 2021. 1999-4923 http://hdl.handle.net/11449/229930 10.3390/pharmaceutics13111902 2-s2.0-85119609400 |
url |
http://dx.doi.org/10.3390/pharmaceutics13111902 http://hdl.handle.net/11449/229930 |
identifier_str_mv |
Pharmaceutics, v. 13, n. 11, 2021. 1999-4923 10.3390/pharmaceutics13111902 2-s2.0-85119609400 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Pharmaceutics |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.source.none.fl_str_mv |
Scopus reponame:Repositório Institucional da UNESP instname:Universidade Estadual Paulista (UNESP) instacron:UNESP |
instname_str |
Universidade Estadual Paulista (UNESP) |
instacron_str |
UNESP |
institution |
UNESP |
reponame_str |
Repositório Institucional da UNESP |
collection |
Repositório Institucional da UNESP |
repository.name.fl_str_mv |
Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP) |
repository.mail.fl_str_mv |
|
_version_ |
1808129304594219008 |