The use of menthol in skin wound healing—anti-inflammatory potential, antioxidant defense system stimulation and increased epithelialization

Detalhes bibliográficos
Autor(a) principal: Rozza, Ariane Leite [UNESP]
Data de Publicação: 2021
Outros Autores: Beserra, Fernando Pereira [UNESP], Vieira, Ana Júlia [UNESP], de Souza, Eduardo Oliveira [UNESP], Hussni, Carlos Alberto [UNESP], Martinez, Emanuel Ricardo Monteiro [UNESP], Nóbrega, Rafael Henrique [UNESP], Pellizzon, Cláudia Helena [UNESP]
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da UNESP
Texto Completo: http://dx.doi.org/10.3390/pharmaceutics13111902
http://hdl.handle.net/11449/229930
Resumo: Wound healing involves inflammatory, proliferative, and remodeling phases, in which various cells and chemical intermediates are involved. This study aimed to investigate the skin wound healing potential of menthol, as well as the mechanisms involved in its effect, after 3, 7, or 14 days of treatment, according to the phases of wound healing. Skin wound was performed in the back of Wistar rats, which were topically treated with vehicle cream; collagenase-based cream (1.2 U/g); or menthol-based cream at 0.25%, 0.5%, or 1.0% over 3, 7, or 14 days. Menthol cream at 0.5% accelerated the healing right from the inflammatory phase (3 days) by decreasing mRNA expression of inflammatory cytokines TNF-α and Il-6. At the proliferative phase (7 days), menthol 0.5% increased the activity of antioxidant enzymes SOD, GR, and GPx, as well as the level of GSH, in addition to decreasing the levels of inflammatory cytokines TNF-α, IL-6, and IL-1β and augmenting mRNA expression for Ki-67, a marker of cellular proliferation. At the remodeling phase (14 days), levels of inflammatory cytokines were decreased, and the level of Il-10 and its mRNA expression were increased in the menthol 0.5% group. Menthol presented skin wound healing activity by modulating the antioxidant system of the cells and the inflammatory response, in addition to stimulating epithelialization.
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spelling The use of menthol in skin wound healing—anti-inflammatory potential, antioxidant defense system stimulation and increased epithelializationAntioxidantGene expressionGSHIL-10InflammationMentholSkin wound healingTNF-αWound healing involves inflammatory, proliferative, and remodeling phases, in which various cells and chemical intermediates are involved. This study aimed to investigate the skin wound healing potential of menthol, as well as the mechanisms involved in its effect, after 3, 7, or 14 days of treatment, according to the phases of wound healing. Skin wound was performed in the back of Wistar rats, which were topically treated with vehicle cream; collagenase-based cream (1.2 U/g); or menthol-based cream at 0.25%, 0.5%, or 1.0% over 3, 7, or 14 days. Menthol cream at 0.5% accelerated the healing right from the inflammatory phase (3 days) by decreasing mRNA expression of inflammatory cytokines TNF-α and Il-6. At the proliferative phase (7 days), menthol 0.5% increased the activity of antioxidant enzymes SOD, GR, and GPx, as well as the level of GSH, in addition to decreasing the levels of inflammatory cytokines TNF-α, IL-6, and IL-1β and augmenting mRNA expression for Ki-67, a marker of cellular proliferation. At the remodeling phase (14 days), levels of inflammatory cytokines were decreased, and the level of Il-10 and its mRNA expression were increased in the menthol 0.5% group. Menthol presented skin wound healing activity by modulating the antioxidant system of the cells and the inflammatory response, in addition to stimulating epithelialization.Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)Department of Structural and Functional Biology Institute of Biosciences São Paulo State University (UNESP), Dr. Antonio Celso W Zanin Street, 250Department of Surgery and Veterinary Anesthesiology School of Veterinary Medicine and Animal Science São Paulo State University (UNESP), Dr. Walter M Correa StreetDepartment of Structural and Functional Biology Institute of Biosciences São Paulo State University (UNESP), Dr. Antonio Celso W Zanin Street, 250Department of Surgery and Veterinary Anesthesiology School of Veterinary Medicine and Animal Science São Paulo State University (UNESP), Dr. Walter M Correa StreetFAPESP: 2013/22202-4FAPESP: 2013/23340-1FAPESP: 2015/06636-0FAPESP: 2016/13307-5CNPq: 406854/2016-5Universidade Estadual Paulista (UNESP)Rozza, Ariane Leite [UNESP]Beserra, Fernando Pereira [UNESP]Vieira, Ana Júlia [UNESP]de Souza, Eduardo Oliveira [UNESP]Hussni, Carlos Alberto [UNESP]Martinez, Emanuel Ricardo Monteiro [UNESP]Nóbrega, Rafael Henrique [UNESP]Pellizzon, Cláudia Helena [UNESP]2022-04-29T08:36:42Z2022-04-29T08:36:42Z2021-11-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articlehttp://dx.doi.org/10.3390/pharmaceutics13111902Pharmaceutics, v. 13, n. 11, 2021.1999-4923http://hdl.handle.net/11449/22993010.3390/pharmaceutics131119022-s2.0-85119609400Scopusreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengPharmaceuticsinfo:eu-repo/semantics/openAccess2022-04-29T08:36:42Zoai:repositorio.unesp.br:11449/229930Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462024-08-05T21:16:39.981818Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false
dc.title.none.fl_str_mv The use of menthol in skin wound healing—anti-inflammatory potential, antioxidant defense system stimulation and increased epithelialization
title The use of menthol in skin wound healing—anti-inflammatory potential, antioxidant defense system stimulation and increased epithelialization
spellingShingle The use of menthol in skin wound healing—anti-inflammatory potential, antioxidant defense system stimulation and increased epithelialization
Rozza, Ariane Leite [UNESP]
Antioxidant
Gene expression
GSH
IL-10
Inflammation
Menthol
Skin wound healing
TNF-α
title_short The use of menthol in skin wound healing—anti-inflammatory potential, antioxidant defense system stimulation and increased epithelialization
title_full The use of menthol in skin wound healing—anti-inflammatory potential, antioxidant defense system stimulation and increased epithelialization
title_fullStr The use of menthol in skin wound healing—anti-inflammatory potential, antioxidant defense system stimulation and increased epithelialization
title_full_unstemmed The use of menthol in skin wound healing—anti-inflammatory potential, antioxidant defense system stimulation and increased epithelialization
title_sort The use of menthol in skin wound healing—anti-inflammatory potential, antioxidant defense system stimulation and increased epithelialization
author Rozza, Ariane Leite [UNESP]
author_facet Rozza, Ariane Leite [UNESP]
Beserra, Fernando Pereira [UNESP]
Vieira, Ana Júlia [UNESP]
de Souza, Eduardo Oliveira [UNESP]
Hussni, Carlos Alberto [UNESP]
Martinez, Emanuel Ricardo Monteiro [UNESP]
Nóbrega, Rafael Henrique [UNESP]
Pellizzon, Cláudia Helena [UNESP]
author_role author
author2 Beserra, Fernando Pereira [UNESP]
Vieira, Ana Júlia [UNESP]
de Souza, Eduardo Oliveira [UNESP]
Hussni, Carlos Alberto [UNESP]
Martinez, Emanuel Ricardo Monteiro [UNESP]
Nóbrega, Rafael Henrique [UNESP]
Pellizzon, Cláudia Helena [UNESP]
author2_role author
author
author
author
author
author
author
dc.contributor.none.fl_str_mv Universidade Estadual Paulista (UNESP)
dc.contributor.author.fl_str_mv Rozza, Ariane Leite [UNESP]
Beserra, Fernando Pereira [UNESP]
Vieira, Ana Júlia [UNESP]
de Souza, Eduardo Oliveira [UNESP]
Hussni, Carlos Alberto [UNESP]
Martinez, Emanuel Ricardo Monteiro [UNESP]
Nóbrega, Rafael Henrique [UNESP]
Pellizzon, Cláudia Helena [UNESP]
dc.subject.por.fl_str_mv Antioxidant
Gene expression
GSH
IL-10
Inflammation
Menthol
Skin wound healing
TNF-α
topic Antioxidant
Gene expression
GSH
IL-10
Inflammation
Menthol
Skin wound healing
TNF-α
description Wound healing involves inflammatory, proliferative, and remodeling phases, in which various cells and chemical intermediates are involved. This study aimed to investigate the skin wound healing potential of menthol, as well as the mechanisms involved in its effect, after 3, 7, or 14 days of treatment, according to the phases of wound healing. Skin wound was performed in the back of Wistar rats, which were topically treated with vehicle cream; collagenase-based cream (1.2 U/g); or menthol-based cream at 0.25%, 0.5%, or 1.0% over 3, 7, or 14 days. Menthol cream at 0.5% accelerated the healing right from the inflammatory phase (3 days) by decreasing mRNA expression of inflammatory cytokines TNF-α and Il-6. At the proliferative phase (7 days), menthol 0.5% increased the activity of antioxidant enzymes SOD, GR, and GPx, as well as the level of GSH, in addition to decreasing the levels of inflammatory cytokines TNF-α, IL-6, and IL-1β and augmenting mRNA expression for Ki-67, a marker of cellular proliferation. At the remodeling phase (14 days), levels of inflammatory cytokines were decreased, and the level of Il-10 and its mRNA expression were increased in the menthol 0.5% group. Menthol presented skin wound healing activity by modulating the antioxidant system of the cells and the inflammatory response, in addition to stimulating epithelialization.
publishDate 2021
dc.date.none.fl_str_mv 2021-11-01
2022-04-29T08:36:42Z
2022-04-29T08:36:42Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://dx.doi.org/10.3390/pharmaceutics13111902
Pharmaceutics, v. 13, n. 11, 2021.
1999-4923
http://hdl.handle.net/11449/229930
10.3390/pharmaceutics13111902
2-s2.0-85119609400
url http://dx.doi.org/10.3390/pharmaceutics13111902
http://hdl.handle.net/11449/229930
identifier_str_mv Pharmaceutics, v. 13, n. 11, 2021.
1999-4923
10.3390/pharmaceutics13111902
2-s2.0-85119609400
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv Pharmaceutics
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.source.none.fl_str_mv Scopus
reponame:Repositório Institucional da UNESP
instname:Universidade Estadual Paulista (UNESP)
instacron:UNESP
instname_str Universidade Estadual Paulista (UNESP)
instacron_str UNESP
institution UNESP
reponame_str Repositório Institucional da UNESP
collection Repositório Institucional da UNESP
repository.name.fl_str_mv Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)
repository.mail.fl_str_mv
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