Cell-mediated immune response to Leishmania chagasi experimental infection of BALB/c immunosuppressed mice
Autor(a) principal: | |
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Data de Publicação: | 2010 |
Outros Autores: | , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UNESP |
Texto Completo: | http://dx.doi.org/10.1590/S1678-91992010000100013 http://hdl.handle.net/11449/13878 |
Resumo: | Leishmaniasis, a zoonosis of worldwide distribution, presents a significant impact on immunosupressed patients. This study aimed to evaluate Leishmania chagasi infection in BALB/c mice immunosuppressed with dexamethasone. Spleen cells stimulated or not with L. chagasi were cultured for cytokine quantification (IFN-gamma, IL-2, IL-4 and IL-10) by sandwich ELISA. Parasite loads in the spleen and liver were determined by means of culture microtitration. Immunosuppressed groups showed statistically lower spleen weight and CD4-cell percentage in blood on the day of infection and produced Th1 and Th2 cytokines on other days of the study. The other infected groups, weather immunosupressed or not, also produced Th1 and Th2 cytokines. Parasite loads in the spleen and liver were not statistically different among the groups. It was concluded that L. chagasi infection was not affected by dexamethasone-induced immunosuppression, probably due the reversible effect of the treatment. |
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Repositório Institucional da UNESP |
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Cell-mediated immune response to Leishmania chagasi experimental infection of BALB/c immunosuppressed micevisceral leishmaniasisimmunosuppressionimmunopathologymiceinterleukinsLeishmaniasis, a zoonosis of worldwide distribution, presents a significant impact on immunosupressed patients. This study aimed to evaluate Leishmania chagasi infection in BALB/c mice immunosuppressed with dexamethasone. Spleen cells stimulated or not with L. chagasi were cultured for cytokine quantification (IFN-gamma, IL-2, IL-4 and IL-10) by sandwich ELISA. Parasite loads in the spleen and liver were determined by means of culture microtitration. Immunosuppressed groups showed statistically lower spleen weight and CD4-cell percentage in blood on the day of infection and produced Th1 and Th2 cytokines on other days of the study. The other infected groups, weather immunosupressed or not, also produced Th1 and Th2 cytokines. Parasite loads in the spleen and liver were not statistically different among the groups. It was concluded that L. chagasi infection was not affected by dexamethasone-induced immunosuppression, probably due the reversible effect of the treatment.Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)São Paulo State Univ, UNESP, Dept Higiene Vet & Saúde Publ,NUPEZO, Zoonosis Res Ctr,Fac Med Vet & Zootecnia,Vet Med, BR-18618000 Botucatu, SP, BrazilSão Paulo State Univ, UNESP, Botucatu Biosci Inst, Dept Microbiol & Immunol, BR-18618000 Botucatu, SP, BrazilSão Paulo State Univ, UNESP, Dept Higiene Vet & Saúde Publ,NUPEZO, Zoonosis Res Ctr,Fac Med Vet & Zootecnia,Vet Med, BR-18618000 Botucatu, SP, BrazilSão Paulo State Univ, UNESP, Botucatu Biosci Inst, Dept Microbiol & Immunol, BR-18618000 Botucatu, SP, BrazilFAPESP: 04/14153-4FAPESP: 04/14762-0Universidade Estadual Paulista (Unesp), Centro de Estudos de Venenos e Animais Peçonhentos (CEVAP)Universidade Estadual Paulista (Unesp)Machado, J. G. [UNESP]Hoffmann, J. L.Krause, V. L. K.da Silva, A.Dias-Melicio, L. A. [UNESP]Langoni, Hélio [UNESP]2014-05-20T13:39:58Z2014-05-20T13:39:58Z2010-01-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article131-146application/pdfhttp://dx.doi.org/10.1590/S1678-91992010000100013Journal of Venomous Animals and Toxins Including Tropical Diseases. Botucatu: Cevap-unesp, v. 16, n. 1, p. 131-146, 2010.1678-9199http://hdl.handle.net/11449/13878S1678-91992010000100013WOS:000276086200013S1678-91992010000100013-en.pdfWeb of Sciencereponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengJournal of Venomous Animals and Toxins Including Tropical Diseases1.7820,573info:eu-repo/semantics/openAccess2023-12-19T06:26:25Zoai:repositorio.unesp.br:11449/13878Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462024-08-05T20:46:40.579192Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false |
dc.title.none.fl_str_mv |
Cell-mediated immune response to Leishmania chagasi experimental infection of BALB/c immunosuppressed mice |
title |
Cell-mediated immune response to Leishmania chagasi experimental infection of BALB/c immunosuppressed mice |
spellingShingle |
Cell-mediated immune response to Leishmania chagasi experimental infection of BALB/c immunosuppressed mice Machado, J. G. [UNESP] visceral leishmaniasis immunosuppression immunopathology mice interleukins |
title_short |
Cell-mediated immune response to Leishmania chagasi experimental infection of BALB/c immunosuppressed mice |
title_full |
Cell-mediated immune response to Leishmania chagasi experimental infection of BALB/c immunosuppressed mice |
title_fullStr |
Cell-mediated immune response to Leishmania chagasi experimental infection of BALB/c immunosuppressed mice |
title_full_unstemmed |
Cell-mediated immune response to Leishmania chagasi experimental infection of BALB/c immunosuppressed mice |
title_sort |
Cell-mediated immune response to Leishmania chagasi experimental infection of BALB/c immunosuppressed mice |
author |
Machado, J. G. [UNESP] |
author_facet |
Machado, J. G. [UNESP] Hoffmann, J. L. Krause, V. L. K. da Silva, A. Dias-Melicio, L. A. [UNESP] Langoni, Hélio [UNESP] |
author_role |
author |
author2 |
Hoffmann, J. L. Krause, V. L. K. da Silva, A. Dias-Melicio, L. A. [UNESP] Langoni, Hélio [UNESP] |
author2_role |
author author author author author |
dc.contributor.none.fl_str_mv |
Universidade Estadual Paulista (Unesp) |
dc.contributor.author.fl_str_mv |
Machado, J. G. [UNESP] Hoffmann, J. L. Krause, V. L. K. da Silva, A. Dias-Melicio, L. A. [UNESP] Langoni, Hélio [UNESP] |
dc.subject.por.fl_str_mv |
visceral leishmaniasis immunosuppression immunopathology mice interleukins |
topic |
visceral leishmaniasis immunosuppression immunopathology mice interleukins |
description |
Leishmaniasis, a zoonosis of worldwide distribution, presents a significant impact on immunosupressed patients. This study aimed to evaluate Leishmania chagasi infection in BALB/c mice immunosuppressed with dexamethasone. Spleen cells stimulated or not with L. chagasi were cultured for cytokine quantification (IFN-gamma, IL-2, IL-4 and IL-10) by sandwich ELISA. Parasite loads in the spleen and liver were determined by means of culture microtitration. Immunosuppressed groups showed statistically lower spleen weight and CD4-cell percentage in blood on the day of infection and produced Th1 and Th2 cytokines on other days of the study. The other infected groups, weather immunosupressed or not, also produced Th1 and Th2 cytokines. Parasite loads in the spleen and liver were not statistically different among the groups. It was concluded that L. chagasi infection was not affected by dexamethasone-induced immunosuppression, probably due the reversible effect of the treatment. |
publishDate |
2010 |
dc.date.none.fl_str_mv |
2010-01-01 2014-05-20T13:39:58Z 2014-05-20T13:39:58Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://dx.doi.org/10.1590/S1678-91992010000100013 Journal of Venomous Animals and Toxins Including Tropical Diseases. Botucatu: Cevap-unesp, v. 16, n. 1, p. 131-146, 2010. 1678-9199 http://hdl.handle.net/11449/13878 S1678-91992010000100013 WOS:000276086200013 S1678-91992010000100013-en.pdf |
url |
http://dx.doi.org/10.1590/S1678-91992010000100013 http://hdl.handle.net/11449/13878 |
identifier_str_mv |
Journal of Venomous Animals and Toxins Including Tropical Diseases. Botucatu: Cevap-unesp, v. 16, n. 1, p. 131-146, 2010. 1678-9199 S1678-91992010000100013 WOS:000276086200013 S1678-91992010000100013-en.pdf |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Journal of Venomous Animals and Toxins Including Tropical Diseases 1.782 0,573 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
131-146 application/pdf |
dc.publisher.none.fl_str_mv |
Universidade Estadual Paulista (Unesp), Centro de Estudos de Venenos e Animais Peçonhentos (CEVAP) |
publisher.none.fl_str_mv |
Universidade Estadual Paulista (Unesp), Centro de Estudos de Venenos e Animais Peçonhentos (CEVAP) |
dc.source.none.fl_str_mv |
Web of Science reponame:Repositório Institucional da UNESP instname:Universidade Estadual Paulista (UNESP) instacron:UNESP |
instname_str |
Universidade Estadual Paulista (UNESP) |
instacron_str |
UNESP |
institution |
UNESP |
reponame_str |
Repositório Institucional da UNESP |
collection |
Repositório Institucional da UNESP |
repository.name.fl_str_mv |
Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP) |
repository.mail.fl_str_mv |
|
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1808129247176294400 |