Study of DNA damage induced by dental bleaching agents in vitro

• Autor(a) principal: Ribeiro, Daniel Araki [UNESP]
• Data de Publicação: 2006
• Outros Autores: Marques, Mariângela Esther Alencar [UNESP], Salvadori, Daisy Maria Favero [UNESP]
• Tipo de documento: Artigo
• Idioma: eng
• Título da fonte: Repositório Institucional da UNESP
• Texto Completo: http://dx.doi.org/10.1590/S1806-83242006000100009; http://hdl.handle.net/11449/68903
• topic: CHO cells; Comet assay; Mutagenicity tests; Tooth bleaching; hydrogen peroxide; oxidizing agent; animal; CHO cell; comet assay; Cricetulus; dental care; DNA damage; drug effect; hamster; in vitro study; nonparametric test; Animals; CHO Cells; Comet Assay; Cricetinae; DNA Damage; Hydrogen Peroxide; Oxidants; Statistics, Nonparametric; Tooth Bleaching

Dental bleaching is a simple and conservative procedure for aesthetic restoration of vital and non-vital discolored teeth. Nevertheless, a number of studies have demonstrated the risk of tissue damage from the contact of these agents with the oral mucosa. In the current study, the genotoxic potential associated with exposure to dental bleaching agents was assessed by the single cell gel (comet) assay in vitro. Chinese hamster ovary (CHO) cells in vitro were exposed to six commercial dental bleaching agents (Clarigel Gold - Dentsply; Whitespeed - Discus Dental; Nite White - Discus Dental; Magic Bleaching - Vigodent; Whiteness HP - FGM and Lase Peroxide - DMC). The results pointed out that all dental bleaching agents tested contributed to DNA damage as depicted by the mean tail moment, being the strongest effect observed with the highest dose of hydrogen peroxide (Whiteness HP and Lase peroxide, at a 35% concentration). On the other hand, Magic Bleaching (Vigodent) induced the lowest level of DNA breakage. Negative and positive controls displayed absence and presence of DNA-damaging, respectively. Taken together, these results suggest that dental bleaching agents may be a factor that increases the level of DNA damage. A higher concentration of hydrogen peroxide produced higher noxious activities in the genome as detected by single cell gel (comet) assay.