Efficacy of photodynamic therapy using TiO2 nanoparticles doped with Zn and hypericin in the treatment of cutaneous Leishmaniasis caused by Leishmania amazonensis
Autor(a) principal: | |
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Data de Publicação: | 2020 |
Outros Autores: | , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UNESP |
Texto Completo: | http://dx.doi.org/10.1016/j.pdpdt.2020.101676 http://hdl.handle.net/11449/201577 |
Resumo: | Since Leishmania parasites exhibit resistance outbreaks to drugs conventionally used in medical treatments, research of new antileishmanial compounds or alternative treatment therapies are essential. A focus of interest has been the implementation of light-based therapies such as photodynamic therapy, where inorganic compounds such as titanium dioxide have shown promising results as drug delivery carriers. In this work, nanoparticles of TiO2 doped with Zn (TiO2/Zn) were synthesized through solution combustion route and with hypericin (HY) in order to enhance its photodynamic activity in the visible light region. Scanning (SEM) and transmission (TEM) electron microscopy analyses showed particles of (TiO2/Zn) with sizes smaller than 20 nm and formation of aggregates smaller than 1 μm, whilst electron diffraction spectroscopy (EDS) analysis ensured the presence of Zn in the system. The association of the TiO2/Zn with HY (TiO2/Zn-HY) was further confirmed by fluorescence spectrometry. Measurements of its cellular uptake showed the presence of smaller molecules into promastigotes after 120 min incubation. TiO2/Zn-HY showed good antileishmanial activity (EC50 of 17.5 ± 0.2 μg mL−1) and low cytotoxicity against murine macrophages (CC50 35.2 ± 0.3 μg mL−1) in the visible light (22 mW cm-2; 52.8 J cm-2). Moreover, in the in vivo analysis, TiO2/Zn-HY decreased the parasite load of L. amazonensis - BALB/c infected mice by 43% – 58% after a combination of blue and red light presenting 22 mW cm-2 of intensity and 52.8 J cm-2 of fluency delivered. All together, these data indicate a new combined system of nanoparticles associated with a photosensitizer and PDT as alternative to amphotericin B for the treatment of cutaneous leishmaniasis. |
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Efficacy of photodynamic therapy using TiO2 nanoparticles doped with Zn and hypericin in the treatment of cutaneous Leishmaniasis caused by Leishmania amazonensisCutaneous leishmaniasisHypericinLeishmania amazonensisNanoparticlesPhotodynamic Therapy (PDT)Reactive oxygen species (ROS)TitaniumSince Leishmania parasites exhibit resistance outbreaks to drugs conventionally used in medical treatments, research of new antileishmanial compounds or alternative treatment therapies are essential. A focus of interest has been the implementation of light-based therapies such as photodynamic therapy, where inorganic compounds such as titanium dioxide have shown promising results as drug delivery carriers. In this work, nanoparticles of TiO2 doped with Zn (TiO2/Zn) were synthesized through solution combustion route and with hypericin (HY) in order to enhance its photodynamic activity in the visible light region. Scanning (SEM) and transmission (TEM) electron microscopy analyses showed particles of (TiO2/Zn) with sizes smaller than 20 nm and formation of aggregates smaller than 1 μm, whilst electron diffraction spectroscopy (EDS) analysis ensured the presence of Zn in the system. The association of the TiO2/Zn with HY (TiO2/Zn-HY) was further confirmed by fluorescence spectrometry. Measurements of its cellular uptake showed the presence of smaller molecules into promastigotes after 120 min incubation. TiO2/Zn-HY showed good antileishmanial activity (EC50 of 17.5 ± 0.2 μg mL−1) and low cytotoxicity against murine macrophages (CC50 35.2 ± 0.3 μg mL−1) in the visible light (22 mW cm-2; 52.8 J cm-2). Moreover, in the in vivo analysis, TiO2/Zn-HY decreased the parasite load of L. amazonensis - BALB/c infected mice by 43% – 58% after a combination of blue and red light presenting 22 mW cm-2 of intensity and 52.8 J cm-2 of fluency delivered. All together, these data indicate a new combined system of nanoparticles associated with a photosensitizer and PDT as alternative to amphotericin B for the treatment of cutaneous leishmaniasis.Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)Universidad Nacional de Colombia Escuela de Física, Carrera 65 Nro. 59A - 110 Grupo de Cerámicos y VítreosInstitución universitaria Pascual Bravo Facultad de Ingeniería Grupo GICEI, calle 73 No. 73 A 226, código postal 050034Universidade Estadual Paulista (UNESP) Faculdade de Ciências FarmacêuticasInstituto de Química de São Carlos Universidade de São PauloUniversidade Estadual Paulista (UNESP) Faculdade de Ciências FarmacêuticasEscuela de FísicaFacultad de Ingeniería Grupo GICEIUniversidade Estadual Paulista (Unesp)Universidade de São Paulo (USP)Sepúlveda, Alex Arbey LoperaArenas Velásquez, Angela Maria [UNESP]Patiño Linares, Irwin Alexanderde Almeida, Leticia [UNESP]Fontana, Carla Raquel [UNESP]Garcia, ClaudiaGraminha, Marcia Aparecida Silva [UNESP]2020-12-12T02:36:15Z2020-12-12T02:36:15Z2020-06-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articlehttp://dx.doi.org/10.1016/j.pdpdt.2020.101676Photodiagnosis and Photodynamic Therapy, v. 30.1873-15971572-1000http://hdl.handle.net/11449/20157710.1016/j.pdpdt.2020.1016762-s2.0-85079880190Scopusreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengPhotodiagnosis and Photodynamic Therapyinfo:eu-repo/semantics/openAccess2024-06-21T15:18:34Zoai:repositorio.unesp.br:11449/201577Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462024-08-05T16:13:33.337002Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false |
dc.title.none.fl_str_mv |
Efficacy of photodynamic therapy using TiO2 nanoparticles doped with Zn and hypericin in the treatment of cutaneous Leishmaniasis caused by Leishmania amazonensis |
title |
Efficacy of photodynamic therapy using TiO2 nanoparticles doped with Zn and hypericin in the treatment of cutaneous Leishmaniasis caused by Leishmania amazonensis |
spellingShingle |
Efficacy of photodynamic therapy using TiO2 nanoparticles doped with Zn and hypericin in the treatment of cutaneous Leishmaniasis caused by Leishmania amazonensis Sepúlveda, Alex Arbey Lopera Cutaneous leishmaniasis Hypericin Leishmania amazonensis Nanoparticles Photodynamic Therapy (PDT) Reactive oxygen species (ROS) Titanium |
title_short |
Efficacy of photodynamic therapy using TiO2 nanoparticles doped with Zn and hypericin in the treatment of cutaneous Leishmaniasis caused by Leishmania amazonensis |
title_full |
Efficacy of photodynamic therapy using TiO2 nanoparticles doped with Zn and hypericin in the treatment of cutaneous Leishmaniasis caused by Leishmania amazonensis |
title_fullStr |
Efficacy of photodynamic therapy using TiO2 nanoparticles doped with Zn and hypericin in the treatment of cutaneous Leishmaniasis caused by Leishmania amazonensis |
title_full_unstemmed |
Efficacy of photodynamic therapy using TiO2 nanoparticles doped with Zn and hypericin in the treatment of cutaneous Leishmaniasis caused by Leishmania amazonensis |
title_sort |
Efficacy of photodynamic therapy using TiO2 nanoparticles doped with Zn and hypericin in the treatment of cutaneous Leishmaniasis caused by Leishmania amazonensis |
author |
Sepúlveda, Alex Arbey Lopera |
author_facet |
Sepúlveda, Alex Arbey Lopera Arenas Velásquez, Angela Maria [UNESP] Patiño Linares, Irwin Alexander de Almeida, Leticia [UNESP] Fontana, Carla Raquel [UNESP] Garcia, Claudia Graminha, Marcia Aparecida Silva [UNESP] |
author_role |
author |
author2 |
Arenas Velásquez, Angela Maria [UNESP] Patiño Linares, Irwin Alexander de Almeida, Leticia [UNESP] Fontana, Carla Raquel [UNESP] Garcia, Claudia Graminha, Marcia Aparecida Silva [UNESP] |
author2_role |
author author author author author author |
dc.contributor.none.fl_str_mv |
Escuela de Física Facultad de Ingeniería Grupo GICEI Universidade Estadual Paulista (Unesp) Universidade de São Paulo (USP) |
dc.contributor.author.fl_str_mv |
Sepúlveda, Alex Arbey Lopera Arenas Velásquez, Angela Maria [UNESP] Patiño Linares, Irwin Alexander de Almeida, Leticia [UNESP] Fontana, Carla Raquel [UNESP] Garcia, Claudia Graminha, Marcia Aparecida Silva [UNESP] |
dc.subject.por.fl_str_mv |
Cutaneous leishmaniasis Hypericin Leishmania amazonensis Nanoparticles Photodynamic Therapy (PDT) Reactive oxygen species (ROS) Titanium |
topic |
Cutaneous leishmaniasis Hypericin Leishmania amazonensis Nanoparticles Photodynamic Therapy (PDT) Reactive oxygen species (ROS) Titanium |
description |
Since Leishmania parasites exhibit resistance outbreaks to drugs conventionally used in medical treatments, research of new antileishmanial compounds or alternative treatment therapies are essential. A focus of interest has been the implementation of light-based therapies such as photodynamic therapy, where inorganic compounds such as titanium dioxide have shown promising results as drug delivery carriers. In this work, nanoparticles of TiO2 doped with Zn (TiO2/Zn) were synthesized through solution combustion route and with hypericin (HY) in order to enhance its photodynamic activity in the visible light region. Scanning (SEM) and transmission (TEM) electron microscopy analyses showed particles of (TiO2/Zn) with sizes smaller than 20 nm and formation of aggregates smaller than 1 μm, whilst electron diffraction spectroscopy (EDS) analysis ensured the presence of Zn in the system. The association of the TiO2/Zn with HY (TiO2/Zn-HY) was further confirmed by fluorescence spectrometry. Measurements of its cellular uptake showed the presence of smaller molecules into promastigotes after 120 min incubation. TiO2/Zn-HY showed good antileishmanial activity (EC50 of 17.5 ± 0.2 μg mL−1) and low cytotoxicity against murine macrophages (CC50 35.2 ± 0.3 μg mL−1) in the visible light (22 mW cm-2; 52.8 J cm-2). Moreover, in the in vivo analysis, TiO2/Zn-HY decreased the parasite load of L. amazonensis - BALB/c infected mice by 43% – 58% after a combination of blue and red light presenting 22 mW cm-2 of intensity and 52.8 J cm-2 of fluency delivered. All together, these data indicate a new combined system of nanoparticles associated with a photosensitizer and PDT as alternative to amphotericin B for the treatment of cutaneous leishmaniasis. |
publishDate |
2020 |
dc.date.none.fl_str_mv |
2020-12-12T02:36:15Z 2020-12-12T02:36:15Z 2020-06-01 |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://dx.doi.org/10.1016/j.pdpdt.2020.101676 Photodiagnosis and Photodynamic Therapy, v. 30. 1873-1597 1572-1000 http://hdl.handle.net/11449/201577 10.1016/j.pdpdt.2020.101676 2-s2.0-85079880190 |
url |
http://dx.doi.org/10.1016/j.pdpdt.2020.101676 http://hdl.handle.net/11449/201577 |
identifier_str_mv |
Photodiagnosis and Photodynamic Therapy, v. 30. 1873-1597 1572-1000 10.1016/j.pdpdt.2020.101676 2-s2.0-85079880190 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Photodiagnosis and Photodynamic Therapy |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.source.none.fl_str_mv |
Scopus reponame:Repositório Institucional da UNESP instname:Universidade Estadual Paulista (UNESP) instacron:UNESP |
instname_str |
Universidade Estadual Paulista (UNESP) |
instacron_str |
UNESP |
institution |
UNESP |
reponame_str |
Repositório Institucional da UNESP |
collection |
Repositório Institucional da UNESP |
repository.name.fl_str_mv |
Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP) |
repository.mail.fl_str_mv |
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_version_ |
1808128620673105920 |