PRP8 intein in cryptic species of Histoplasma capsulatum: Evolution and phylogeny
Autor(a) principal: | |
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Data de Publicação: | 2013 |
Outros Autores: | , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UNESP |
Texto Completo: | http://dx.doi.org/10.1016/j.meegid.2013.05.001 http://hdl.handle.net/11449/76140 |
Resumo: | The PRP8 intein is the most widespread intein among the Kingdom Fungi. This genetic element occurs within the prp8 gene, and is transcribed and translated simultaneously with the gene. After translation, the intein excises itself from the Prp8 protein by an autocatalytic splicing reaction, subsequently joining the N and C terminals of the host protein, which retains its functional conformation. Besides the splicing domain, some PRP8 inteins also have a homing endonuclease (HE) domain which, if functional, makes the intein a mobile element capable of becoming fixed in a population. This work aimed to study (1) The occurrence of this intein in Histoplasma capsulatum isolates (n=. 99) belonging to different cryptic species collected in diverse geographical locations, and (2) The functionality of the endonuclease domains of H. capsulatum PRP8 inteins and their phylogenetic relationship among the cryptic species. Our results suggest that the PRP8 intein is fixed in H. capsulatum populations and that an admixture or a probable ancestral polymorphism of the PRP8 intein sequences is responsible for the apparent paraphyletic pattern of the LAmA clade which, in the intein phylogeny, also encompasses sequences from LAmB isolates. The PRP8 intein sequences clearly separate the different cryptic species, and may serve as an additional molecular typing tool, as previously proposed for other fungi genus, such as Cryptococcus and Paracoccidioides. © 2013 Elsevier B.V. |
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PRP8 intein in cryptic species of Histoplasma capsulatum: Evolution and phylogenyHistoplasma capsulatumLatin AmericaPhylogenyPRP8 inteinendonucleaseinteinunclassified drugamino acid sequencecladisticscontrolled studyenzyme activityenzyme structurefungus isolationgeographic distributionmolecular evolutionmultilocus sequence typingnonhumannucleotide sequencephylogenypriority journalprotein processingsequence alignmentspecies differenceThe PRP8 intein is the most widespread intein among the Kingdom Fungi. This genetic element occurs within the prp8 gene, and is transcribed and translated simultaneously with the gene. After translation, the intein excises itself from the Prp8 protein by an autocatalytic splicing reaction, subsequently joining the N and C terminals of the host protein, which retains its functional conformation. Besides the splicing domain, some PRP8 inteins also have a homing endonuclease (HE) domain which, if functional, makes the intein a mobile element capable of becoming fixed in a population. This work aimed to study (1) The occurrence of this intein in Histoplasma capsulatum isolates (n=. 99) belonging to different cryptic species collected in diverse geographical locations, and (2) The functionality of the endonuclease domains of H. capsulatum PRP8 inteins and their phylogenetic relationship among the cryptic species. Our results suggest that the PRP8 intein is fixed in H. capsulatum populations and that an admixture or a probable ancestral polymorphism of the PRP8 intein sequences is responsible for the apparent paraphyletic pattern of the LAmA clade which, in the intein phylogeny, also encompasses sequences from LAmB isolates. The PRP8 intein sequences clearly separate the different cryptic species, and may serve as an additional molecular typing tool, as previously proposed for other fungi genus, such as Cryptococcus and Paracoccidioides. © 2013 Elsevier B.V.Universidade Federal do Rio Grande do Norte (UFRN), Campus de Natal, RNMycotic Diseases Branch Centers for Disease Control and Prevention (CDC), Atlanta, GAUnited States Dept. of Agriculture-Agricultural Research Service (USDA-ARS), Davis, CAUniversidade Estadual Paulista (UNESP), Campus de Botucatu, SPUniversidade Estadual Paulista (UNESP), Campus de Botucatu, SPUniversidade Federal do Rio Grande do Norte (UFRN)Centers for Disease Control and Prevention (CDC)United States Dept. of Agriculture-Agricultural Research Service (USDA-ARS)Universidade Estadual Paulista (Unesp)Theodoro, Raquel CordeiroScheel, Christina M.Brandt, Mary E.Kasuga, TakaoBagagli, Eduardo [UNESP]2014-05-27T11:30:05Z2014-05-27T11:30:05Z2013-08-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article174-182application/pdfhttp://dx.doi.org/10.1016/j.meegid.2013.05.001Infection, Genetics and Evolution, v. 18, p. 174-182.1567-13481567-7257http://hdl.handle.net/11449/7614010.1016/j.meegid.2013.05.0012-s2.0-848793878152-s2.0-84879387815.pdf33203275704295390000-0002-8003-41090000-0002-8003-4109Scopusreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengInfection, Genetics and Evolution2.5451,278info:eu-repo/semantics/openAccess2023-11-13T06:14:49Zoai:repositorio.unesp.br:11449/76140Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462024-08-05T17:35:51.348615Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false |
dc.title.none.fl_str_mv |
PRP8 intein in cryptic species of Histoplasma capsulatum: Evolution and phylogeny |
title |
PRP8 intein in cryptic species of Histoplasma capsulatum: Evolution and phylogeny |
spellingShingle |
PRP8 intein in cryptic species of Histoplasma capsulatum: Evolution and phylogeny Theodoro, Raquel Cordeiro Histoplasma capsulatum Latin America Phylogeny PRP8 intein endonuclease intein unclassified drug amino acid sequence cladistics controlled study enzyme activity enzyme structure fungus isolation geographic distribution molecular evolution multilocus sequence typing nonhuman nucleotide sequence phylogeny priority journal protein processing sequence alignment species difference |
title_short |
PRP8 intein in cryptic species of Histoplasma capsulatum: Evolution and phylogeny |
title_full |
PRP8 intein in cryptic species of Histoplasma capsulatum: Evolution and phylogeny |
title_fullStr |
PRP8 intein in cryptic species of Histoplasma capsulatum: Evolution and phylogeny |
title_full_unstemmed |
PRP8 intein in cryptic species of Histoplasma capsulatum: Evolution and phylogeny |
title_sort |
PRP8 intein in cryptic species of Histoplasma capsulatum: Evolution and phylogeny |
author |
Theodoro, Raquel Cordeiro |
author_facet |
Theodoro, Raquel Cordeiro Scheel, Christina M. Brandt, Mary E. Kasuga, Takao Bagagli, Eduardo [UNESP] |
author_role |
author |
author2 |
Scheel, Christina M. Brandt, Mary E. Kasuga, Takao Bagagli, Eduardo [UNESP] |
author2_role |
author author author author |
dc.contributor.none.fl_str_mv |
Universidade Federal do Rio Grande do Norte (UFRN) Centers for Disease Control and Prevention (CDC) United States Dept. of Agriculture-Agricultural Research Service (USDA-ARS) Universidade Estadual Paulista (Unesp) |
dc.contributor.author.fl_str_mv |
Theodoro, Raquel Cordeiro Scheel, Christina M. Brandt, Mary E. Kasuga, Takao Bagagli, Eduardo [UNESP] |
dc.subject.por.fl_str_mv |
Histoplasma capsulatum Latin America Phylogeny PRP8 intein endonuclease intein unclassified drug amino acid sequence cladistics controlled study enzyme activity enzyme structure fungus isolation geographic distribution molecular evolution multilocus sequence typing nonhuman nucleotide sequence phylogeny priority journal protein processing sequence alignment species difference |
topic |
Histoplasma capsulatum Latin America Phylogeny PRP8 intein endonuclease intein unclassified drug amino acid sequence cladistics controlled study enzyme activity enzyme structure fungus isolation geographic distribution molecular evolution multilocus sequence typing nonhuman nucleotide sequence phylogeny priority journal protein processing sequence alignment species difference |
description |
The PRP8 intein is the most widespread intein among the Kingdom Fungi. This genetic element occurs within the prp8 gene, and is transcribed and translated simultaneously with the gene. After translation, the intein excises itself from the Prp8 protein by an autocatalytic splicing reaction, subsequently joining the N and C terminals of the host protein, which retains its functional conformation. Besides the splicing domain, some PRP8 inteins also have a homing endonuclease (HE) domain which, if functional, makes the intein a mobile element capable of becoming fixed in a population. This work aimed to study (1) The occurrence of this intein in Histoplasma capsulatum isolates (n=. 99) belonging to different cryptic species collected in diverse geographical locations, and (2) The functionality of the endonuclease domains of H. capsulatum PRP8 inteins and their phylogenetic relationship among the cryptic species. Our results suggest that the PRP8 intein is fixed in H. capsulatum populations and that an admixture or a probable ancestral polymorphism of the PRP8 intein sequences is responsible for the apparent paraphyletic pattern of the LAmA clade which, in the intein phylogeny, also encompasses sequences from LAmB isolates. The PRP8 intein sequences clearly separate the different cryptic species, and may serve as an additional molecular typing tool, as previously proposed for other fungi genus, such as Cryptococcus and Paracoccidioides. © 2013 Elsevier B.V. |
publishDate |
2013 |
dc.date.none.fl_str_mv |
2013-08-01 2014-05-27T11:30:05Z 2014-05-27T11:30:05Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://dx.doi.org/10.1016/j.meegid.2013.05.001 Infection, Genetics and Evolution, v. 18, p. 174-182. 1567-1348 1567-7257 http://hdl.handle.net/11449/76140 10.1016/j.meegid.2013.05.001 2-s2.0-84879387815 2-s2.0-84879387815.pdf 3320327570429539 0000-0002-8003-4109 0000-0002-8003-4109 |
url |
http://dx.doi.org/10.1016/j.meegid.2013.05.001 http://hdl.handle.net/11449/76140 |
identifier_str_mv |
Infection, Genetics and Evolution, v. 18, p. 174-182. 1567-1348 1567-7257 10.1016/j.meegid.2013.05.001 2-s2.0-84879387815 2-s2.0-84879387815.pdf 3320327570429539 0000-0002-8003-4109 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Infection, Genetics and Evolution 2.545 1,278 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
174-182 application/pdf |
dc.source.none.fl_str_mv |
Scopus reponame:Repositório Institucional da UNESP instname:Universidade Estadual Paulista (UNESP) instacron:UNESP |
instname_str |
Universidade Estadual Paulista (UNESP) |
instacron_str |
UNESP |
institution |
UNESP |
reponame_str |
Repositório Institucional da UNESP |
collection |
Repositório Institucional da UNESP |
repository.name.fl_str_mv |
Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP) |
repository.mail.fl_str_mv |
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1808128832230653952 |