In vitro and in vivo investigation of the genotoxic potential of waters from rivers under the influence of a petroleum refinery (São Paulo State – Brazil)
Autor(a) principal: | |
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Data de Publicação: | 2017 |
Outros Autores: | |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UNESP |
Texto Completo: | http://dx.doi.org/10.1016/j.chemosphere.2017.01.142 http://hdl.handle.net/11449/169433 |
Resumo: | In recent years concern about the chemical composition of wastewater generated by the oil refining industry has increased, even after its treatment. These wastewaters contain substances that can harm both the entire aquatic ecosystem and the health of any exposed organisms. The aim of this study was to evaluate the genotoxic and mutagenic potentials of the effluent generated by the largest Brazilian petroleum refinery, the effectiveness of the treatments used by the refinery, and whether its effluent can compromise the water quality of the river where it is discarded. Chromosomal aberration and micronucleus assays were performed in Allium cepa and micronucleus test in mammalian cell culture (CHO-K1). The samples were collected in three sites at the refinery: one site on the Jaguari River and two sites on the Atibaia Rivers (upstream and downstream of the discharged effluent), under three different climatic conditions. Tests with A. cepa showed increased frequencies of chromosomal aberrations and micronuclei in meristematic cells for the effluent after physico-chemical treatment, but the samples after treatment biological and stabilization pond presented none of these abnormalities. It was observed that the induced damage in the meristematic cells was not observed in the F1cells of A. cepa roots. The micronucleus test performed with mammalian cell culture also indicated that the effluent, after physico-chemical treatment, induced a significant increase in micronucleus frequencies. Plant and hamster cells exposed to the other samples collected inside the refinery and in the Jaguari and Atibaia Rivers did not present evidence of genotoxicity and mutagenicity in the tests performed. This study showed that the effluent treated carried out by the refinery (biological treatment followed by a stabilization pond) proved to be efficient for the removal of the toxic load still present after the physico-chemical treatment, since no change in the quality of the Atibaia River was observed. However, because this is an industry with a high production of effluent with toxic potential, its effluents must be constantly monitored, so that there is no compromise of the water quality of the receiving river. |
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In vitro and in vivo investigation of the genotoxic potential of waters from rivers under the influence of a petroleum refinery (São Paulo State – Brazil)Allium cepaCHO cell cultureChromosomic aberrationsHPAMicronucleusRefinery effluentIn recent years concern about the chemical composition of wastewater generated by the oil refining industry has increased, even after its treatment. These wastewaters contain substances that can harm both the entire aquatic ecosystem and the health of any exposed organisms. The aim of this study was to evaluate the genotoxic and mutagenic potentials of the effluent generated by the largest Brazilian petroleum refinery, the effectiveness of the treatments used by the refinery, and whether its effluent can compromise the water quality of the river where it is discarded. Chromosomal aberration and micronucleus assays were performed in Allium cepa and micronucleus test in mammalian cell culture (CHO-K1). The samples were collected in three sites at the refinery: one site on the Jaguari River and two sites on the Atibaia Rivers (upstream and downstream of the discharged effluent), under three different climatic conditions. Tests with A. cepa showed increased frequencies of chromosomal aberrations and micronuclei in meristematic cells for the effluent after physico-chemical treatment, but the samples after treatment biological and stabilization pond presented none of these abnormalities. It was observed that the induced damage in the meristematic cells was not observed in the F1cells of A. cepa roots. The micronucleus test performed with mammalian cell culture also indicated that the effluent, after physico-chemical treatment, induced a significant increase in micronucleus frequencies. Plant and hamster cells exposed to the other samples collected inside the refinery and in the Jaguari and Atibaia Rivers did not present evidence of genotoxicity and mutagenicity in the tests performed. This study showed that the effluent treated carried out by the refinery (biological treatment followed by a stabilization pond) proved to be efficient for the removal of the toxic load still present after the physico-chemical treatment, since no change in the quality of the Atibaia River was observed. However, because this is an industry with a high production of effluent with toxic potential, its effluents must be constantly monitored, so that there is no compromise of the water quality of the receiving river.Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)UNESP – Institute of Biosciences Department of Biology, Av 24-A, 1515UNESP – Institute of Biosciences Department of Biology, Av 24-A, 1515FAPESP: 2010/13993-0Universidade Estadual Paulista (Unesp)Hara, Raquel Vaz [UNESP]Marin-Morales, Maria Aparecida [UNESP]2018-12-11T16:45:52Z2018-12-11T16:45:52Z2017-01-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article321-330application/pdfhttp://dx.doi.org/10.1016/j.chemosphere.2017.01.142Chemosphere, v. 174, p. 321-330.1879-12980045-6535http://hdl.handle.net/11449/16943310.1016/j.chemosphere.2017.01.1422-s2.0-850117021152-s2.0-85011702115.pdfScopusreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengChemosphere1,435info:eu-repo/semantics/openAccess2023-10-28T06:08:58Zoai:repositorio.unesp.br:11449/169433Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462023-10-28T06:08:58Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false |
dc.title.none.fl_str_mv |
In vitro and in vivo investigation of the genotoxic potential of waters from rivers under the influence of a petroleum refinery (São Paulo State – Brazil) |
title |
In vitro and in vivo investigation of the genotoxic potential of waters from rivers under the influence of a petroleum refinery (São Paulo State – Brazil) |
spellingShingle |
In vitro and in vivo investigation of the genotoxic potential of waters from rivers under the influence of a petroleum refinery (São Paulo State – Brazil) Hara, Raquel Vaz [UNESP] Allium cepa CHO cell culture Chromosomic aberrations HPA Micronucleus Refinery effluent |
title_short |
In vitro and in vivo investigation of the genotoxic potential of waters from rivers under the influence of a petroleum refinery (São Paulo State – Brazil) |
title_full |
In vitro and in vivo investigation of the genotoxic potential of waters from rivers under the influence of a petroleum refinery (São Paulo State – Brazil) |
title_fullStr |
In vitro and in vivo investigation of the genotoxic potential of waters from rivers under the influence of a petroleum refinery (São Paulo State – Brazil) |
title_full_unstemmed |
In vitro and in vivo investigation of the genotoxic potential of waters from rivers under the influence of a petroleum refinery (São Paulo State – Brazil) |
title_sort |
In vitro and in vivo investigation of the genotoxic potential of waters from rivers under the influence of a petroleum refinery (São Paulo State – Brazil) |
author |
Hara, Raquel Vaz [UNESP] |
author_facet |
Hara, Raquel Vaz [UNESP] Marin-Morales, Maria Aparecida [UNESP] |
author_role |
author |
author2 |
Marin-Morales, Maria Aparecida [UNESP] |
author2_role |
author |
dc.contributor.none.fl_str_mv |
Universidade Estadual Paulista (Unesp) |
dc.contributor.author.fl_str_mv |
Hara, Raquel Vaz [UNESP] Marin-Morales, Maria Aparecida [UNESP] |
dc.subject.por.fl_str_mv |
Allium cepa CHO cell culture Chromosomic aberrations HPA Micronucleus Refinery effluent |
topic |
Allium cepa CHO cell culture Chromosomic aberrations HPA Micronucleus Refinery effluent |
description |
In recent years concern about the chemical composition of wastewater generated by the oil refining industry has increased, even after its treatment. These wastewaters contain substances that can harm both the entire aquatic ecosystem and the health of any exposed organisms. The aim of this study was to evaluate the genotoxic and mutagenic potentials of the effluent generated by the largest Brazilian petroleum refinery, the effectiveness of the treatments used by the refinery, and whether its effluent can compromise the water quality of the river where it is discarded. Chromosomal aberration and micronucleus assays were performed in Allium cepa and micronucleus test in mammalian cell culture (CHO-K1). The samples were collected in three sites at the refinery: one site on the Jaguari River and two sites on the Atibaia Rivers (upstream and downstream of the discharged effluent), under three different climatic conditions. Tests with A. cepa showed increased frequencies of chromosomal aberrations and micronuclei in meristematic cells for the effluent after physico-chemical treatment, but the samples after treatment biological and stabilization pond presented none of these abnormalities. It was observed that the induced damage in the meristematic cells was not observed in the F1cells of A. cepa roots. The micronucleus test performed with mammalian cell culture also indicated that the effluent, after physico-chemical treatment, induced a significant increase in micronucleus frequencies. Plant and hamster cells exposed to the other samples collected inside the refinery and in the Jaguari and Atibaia Rivers did not present evidence of genotoxicity and mutagenicity in the tests performed. This study showed that the effluent treated carried out by the refinery (biological treatment followed by a stabilization pond) proved to be efficient for the removal of the toxic load still present after the physico-chemical treatment, since no change in the quality of the Atibaia River was observed. However, because this is an industry with a high production of effluent with toxic potential, its effluents must be constantly monitored, so that there is no compromise of the water quality of the receiving river. |
publishDate |
2017 |
dc.date.none.fl_str_mv |
2017-01-01 2018-12-11T16:45:52Z 2018-12-11T16:45:52Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://dx.doi.org/10.1016/j.chemosphere.2017.01.142 Chemosphere, v. 174, p. 321-330. 1879-1298 0045-6535 http://hdl.handle.net/11449/169433 10.1016/j.chemosphere.2017.01.142 2-s2.0-85011702115 2-s2.0-85011702115.pdf |
url |
http://dx.doi.org/10.1016/j.chemosphere.2017.01.142 http://hdl.handle.net/11449/169433 |
identifier_str_mv |
Chemosphere, v. 174, p. 321-330. 1879-1298 0045-6535 10.1016/j.chemosphere.2017.01.142 2-s2.0-85011702115 2-s2.0-85011702115.pdf |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Chemosphere 1,435 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
321-330 application/pdf |
dc.source.none.fl_str_mv |
Scopus reponame:Repositório Institucional da UNESP instname:Universidade Estadual Paulista (UNESP) instacron:UNESP |
instname_str |
Universidade Estadual Paulista (UNESP) |
instacron_str |
UNESP |
institution |
UNESP |
reponame_str |
Repositório Institucional da UNESP |
collection |
Repositório Institucional da UNESP |
repository.name.fl_str_mv |
Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP) |
repository.mail.fl_str_mv |
|
_version_ |
1799964733517856768 |