In vitro effect of amorphous calcium phosphate paste applied for extended periods of time on enamel remineralization

• Autor(a) principal: Mello Vieira, Ana Elisa de [UNESP]
• Data de Publicação: 2017
• Outros Autores: Danelon, Marcelle [UNESP], Camara, Danielle Mendes da [UNESP], Rosselli, Eliana Rodrigues [UNESP], Stock, Stuart R., Cannon, Mark L., Xiao, Xianghui, De Carlo, Francesco, Botazzo Delbem, Alberto Carlos [UNESP]
• Tipo de documento: Artigo
• Idioma: eng
• Título da fonte: Repositório Institucional da UNESP
• Texto Completo: http://dx.doi.org/10.1590/1678-7757-2016-0513; http://hdl.handle.net/11449/163569
• topic: X-ray microtomography; Tooth remineralization; Calcium phosphates; Fluoride; Dental enamel

Dental applications based on the unique characteristics of amorphous calcium phosphate stabilized by casein phosphopeptides (CPP-ACP) have been proposed, as well as the improvement of its properties. Objectives: The objective of this study was to determine the ability of topically applied CPP-ACP from a commercial product to remineralize subsurface lesions when applied for extended periods of time (3 h and 8 h). Material and Methods: Artificially induced carious lesions were produced in 50 bovine enamel blocks previously selected by surface hardness. After treatments with gel without F and CPP-ACP applied for 1 minute (Placebo); 2% NaF neutral gel applied for 1 minute (Fluoride 1 min); CPP-ACP applied for 3 min (ACP 3 min); and CPP-ACP applied for 3 h (ACP 3 h) and for 8 h (ACP 8 h), the enamel blocks were submitted to the remineralization pH-cycling. Surface hardness and synchrotron micro-tomography were used to determine the percentage of surface hardness recovery (% SHR) and to calculate mineral concentration (g(HAp). cm(-3)), respectively. The data were submitted to ANOVA followed by the Student-Newman-Keuls test (p<0.05). Results: Fluoride gel presented higher % SHR followed by ACP 3 min (p<0.001). No difference (p=0.148) was found for Placebo, ACP 3 h and ACP 8 h groups for % SHR. Fluoride gel showed greater mineral concentration (p<0.001) when compared with the other groups. ACP 3 min demonstrated a significant difference (p<0.001) from ACP 3 h and ACP 8 h. The ACP 3 h and 8 h presented a subsurface lesion with development of laminations in all blocks. Conclusion: In this in vitro study the use of CPP-ACP for extended periods of time did not produce an additive effect in the remineralization process.