In vitro effect of amorphous calcium phosphate paste applied for extended periods of time on enamel remineralization
Autor(a) principal: | |
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Data de Publicação: | 2017 |
Outros Autores: | , , , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UNESP |
DOI: | 10.1590/1678-7757-2016-0513 |
Texto Completo: | http://dx.doi.org/10.1590/1678-7757-2016-0513 http://hdl.handle.net/11449/163569 |
Resumo: | Dental applications based on the unique characteristics of amorphous calcium phosphate stabilized by casein phosphopeptides (CPP-ACP) have been proposed, as well as the improvement of its properties. Objectives: The objective of this study was to determine the ability of topically applied CPP-ACP from a commercial product to remineralize subsurface lesions when applied for extended periods of time (3 h and 8 h). Material and Methods: Artificially induced carious lesions were produced in 50 bovine enamel blocks previously selected by surface hardness. After treatments with gel without F and CPP-ACP applied for 1 minute (Placebo); 2% NaF neutral gel applied for 1 minute (Fluoride 1 min); CPP-ACP applied for 3 min (ACP 3 min); and CPP-ACP applied for 3 h (ACP 3 h) and for 8 h (ACP 8 h), the enamel blocks were submitted to the remineralization pH-cycling. Surface hardness and synchrotron micro-tomography were used to determine the percentage of surface hardness recovery (% SHR) and to calculate mineral concentration (g(HAp). cm(-3)), respectively. The data were submitted to ANOVA followed by the Student-Newman-Keuls test (p<0.05). Results: Fluoride gel presented higher % SHR followed by ACP 3 min (p<0.001). No difference (p=0.148) was found for Placebo, ACP 3 h and ACP 8 h groups for % SHR. Fluoride gel showed greater mineral concentration (p<0.001) when compared with the other groups. ACP 3 min demonstrated a significant difference (p<0.001) from ACP 3 h and ACP 8 h. The ACP 3 h and 8 h presented a subsurface lesion with development of laminations in all blocks. Conclusion: In this in vitro study the use of CPP-ACP for extended periods of time did not produce an additive effect in the remineralization process. |
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In vitro effect of amorphous calcium phosphate paste applied for extended periods of time on enamel remineralizationX-ray microtomographyTooth remineralizationCalcium phosphatesFluorideDental enamelDental applications based on the unique characteristics of amorphous calcium phosphate stabilized by casein phosphopeptides (CPP-ACP) have been proposed, as well as the improvement of its properties. Objectives: The objective of this study was to determine the ability of topically applied CPP-ACP from a commercial product to remineralize subsurface lesions when applied for extended periods of time (3 h and 8 h). Material and Methods: Artificially induced carious lesions were produced in 50 bovine enamel blocks previously selected by surface hardness. After treatments with gel without F and CPP-ACP applied for 1 minute (Placebo); 2% NaF neutral gel applied for 1 minute (Fluoride 1 min); CPP-ACP applied for 3 min (ACP 3 min); and CPP-ACP applied for 3 h (ACP 3 h) and for 8 h (ACP 8 h), the enamel blocks were submitted to the remineralization pH-cycling. Surface hardness and synchrotron micro-tomography were used to determine the percentage of surface hardness recovery (% SHR) and to calculate mineral concentration (g(HAp). cm(-3)), respectively. The data were submitted to ANOVA followed by the Student-Newman-Keuls test (p<0.05). Results: Fluoride gel presented higher % SHR followed by ACP 3 min (p<0.001). No difference (p=0.148) was found for Placebo, ACP 3 h and ACP 8 h groups for % SHR. Fluoride gel showed greater mineral concentration (p<0.001) when compared with the other groups. ACP 3 min demonstrated a significant difference (p<0.001) from ACP 3 h and ACP 8 h. The ACP 3 h and 8 h presented a subsurface lesion with development of laminations in all blocks. Conclusion: In this in vitro study the use of CPP-ACP for extended periods of time did not produce an additive effect in the remineralization process.U.S. Department of Energy, Office of Science, Office of Basic Energy SciencesUniv Estadual Paulista, Fac Odontol Aracatuba, Dept Odontol Infantil & Social, Aracatuba, SP, BrazilNorthwestern Univ, Feinberg Sch Med, Dept Mol Pharmacol & Biol Chem, Chicago, IL 60611 USANorthwestern Univ, Feinberg Sch Med, Ann & Robert Lurie Childrens Hosp, Chicago, IL 60611 USAArgonne Natl Lab, Adv Photon Source, Argonne, IL 60439 USAUniv Estadual Paulista, Fac Odontol Aracatuba, Dept Odontol Infantil & Social, Aracatuba, SP, BrazilU.S. Department of Energy, Office of Science, Office of Basic Energy Sciences: DE-AC02-06CH11357Univ Sao Paulo Fac Odontologia BauruUniversidade Estadual Paulista (Unesp)Northwestern UnivArgonne Natl LabMello Vieira, Ana Elisa de [UNESP]Danelon, Marcelle [UNESP]Camara, Danielle Mendes da [UNESP]Rosselli, Eliana Rodrigues [UNESP]Stock, Stuart R.Cannon, Mark L.Xiao, XianghuiDe Carlo, FrancescoBotazzo Delbem, Alberto Carlos [UNESP]2018-11-26T17:42:34Z2018-11-26T17:42:34Z2017-11-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article596-603application/pdfhttp://dx.doi.org/10.1590/1678-7757-2016-0513Journal Of Applied Oral Science. Bauru-sp: Univ Sao Paulo Fac Odontologia Bauru, v. 25, n. 6, p. 596-603, 2017.1678-7757http://hdl.handle.net/11449/16356910.1590/1678-7757-2016-0513S1678-77572017000600596WOS:000417175900003S1678-77572017000600596.pdfWeb of Sciencereponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengJournal Of Applied Oral Science0,645info:eu-repo/semantics/openAccess2024-09-19T17:56:59Zoai:repositorio.unesp.br:11449/163569Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestrepositoriounesp@unesp.bropendoar:29462024-09-19T17:56:59Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false |
dc.title.none.fl_str_mv |
In vitro effect of amorphous calcium phosphate paste applied for extended periods of time on enamel remineralization |
title |
In vitro effect of amorphous calcium phosphate paste applied for extended periods of time on enamel remineralization |
spellingShingle |
In vitro effect of amorphous calcium phosphate paste applied for extended periods of time on enamel remineralization In vitro effect of amorphous calcium phosphate paste applied for extended periods of time on enamel remineralization Mello Vieira, Ana Elisa de [UNESP] X-ray microtomography Tooth remineralization Calcium phosphates Fluoride Dental enamel Mello Vieira, Ana Elisa de [UNESP] X-ray microtomography Tooth remineralization Calcium phosphates Fluoride Dental enamel |
title_short |
In vitro effect of amorphous calcium phosphate paste applied for extended periods of time on enamel remineralization |
title_full |
In vitro effect of amorphous calcium phosphate paste applied for extended periods of time on enamel remineralization |
title_fullStr |
In vitro effect of amorphous calcium phosphate paste applied for extended periods of time on enamel remineralization In vitro effect of amorphous calcium phosphate paste applied for extended periods of time on enamel remineralization |
title_full_unstemmed |
In vitro effect of amorphous calcium phosphate paste applied for extended periods of time on enamel remineralization In vitro effect of amorphous calcium phosphate paste applied for extended periods of time on enamel remineralization |
title_sort |
In vitro effect of amorphous calcium phosphate paste applied for extended periods of time on enamel remineralization |
author |
Mello Vieira, Ana Elisa de [UNESP] |
author_facet |
Mello Vieira, Ana Elisa de [UNESP] Mello Vieira, Ana Elisa de [UNESP] Danelon, Marcelle [UNESP] Camara, Danielle Mendes da [UNESP] Rosselli, Eliana Rodrigues [UNESP] Stock, Stuart R. Cannon, Mark L. Xiao, Xianghui De Carlo, Francesco Botazzo Delbem, Alberto Carlos [UNESP] Danelon, Marcelle [UNESP] Camara, Danielle Mendes da [UNESP] Rosselli, Eliana Rodrigues [UNESP] Stock, Stuart R. Cannon, Mark L. Xiao, Xianghui De Carlo, Francesco Botazzo Delbem, Alberto Carlos [UNESP] |
author_role |
author |
author2 |
Danelon, Marcelle [UNESP] Camara, Danielle Mendes da [UNESP] Rosselli, Eliana Rodrigues [UNESP] Stock, Stuart R. Cannon, Mark L. Xiao, Xianghui De Carlo, Francesco Botazzo Delbem, Alberto Carlos [UNESP] |
author2_role |
author author author author author author author author |
dc.contributor.none.fl_str_mv |
Universidade Estadual Paulista (Unesp) Northwestern Univ Argonne Natl Lab |
dc.contributor.author.fl_str_mv |
Mello Vieira, Ana Elisa de [UNESP] Danelon, Marcelle [UNESP] Camara, Danielle Mendes da [UNESP] Rosselli, Eliana Rodrigues [UNESP] Stock, Stuart R. Cannon, Mark L. Xiao, Xianghui De Carlo, Francesco Botazzo Delbem, Alberto Carlos [UNESP] |
dc.subject.por.fl_str_mv |
X-ray microtomography Tooth remineralization Calcium phosphates Fluoride Dental enamel |
topic |
X-ray microtomography Tooth remineralization Calcium phosphates Fluoride Dental enamel |
description |
Dental applications based on the unique characteristics of amorphous calcium phosphate stabilized by casein phosphopeptides (CPP-ACP) have been proposed, as well as the improvement of its properties. Objectives: The objective of this study was to determine the ability of topically applied CPP-ACP from a commercial product to remineralize subsurface lesions when applied for extended periods of time (3 h and 8 h). Material and Methods: Artificially induced carious lesions were produced in 50 bovine enamel blocks previously selected by surface hardness. After treatments with gel without F and CPP-ACP applied for 1 minute (Placebo); 2% NaF neutral gel applied for 1 minute (Fluoride 1 min); CPP-ACP applied for 3 min (ACP 3 min); and CPP-ACP applied for 3 h (ACP 3 h) and for 8 h (ACP 8 h), the enamel blocks were submitted to the remineralization pH-cycling. Surface hardness and synchrotron micro-tomography were used to determine the percentage of surface hardness recovery (% SHR) and to calculate mineral concentration (g(HAp). cm(-3)), respectively. The data were submitted to ANOVA followed by the Student-Newman-Keuls test (p<0.05). Results: Fluoride gel presented higher % SHR followed by ACP 3 min (p<0.001). No difference (p=0.148) was found for Placebo, ACP 3 h and ACP 8 h groups for % SHR. Fluoride gel showed greater mineral concentration (p<0.001) when compared with the other groups. ACP 3 min demonstrated a significant difference (p<0.001) from ACP 3 h and ACP 8 h. The ACP 3 h and 8 h presented a subsurface lesion with development of laminations in all blocks. Conclusion: In this in vitro study the use of CPP-ACP for extended periods of time did not produce an additive effect in the remineralization process. |
publishDate |
2017 |
dc.date.none.fl_str_mv |
2017-11-01 2018-11-26T17:42:34Z 2018-11-26T17:42:34Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://dx.doi.org/10.1590/1678-7757-2016-0513 Journal Of Applied Oral Science. Bauru-sp: Univ Sao Paulo Fac Odontologia Bauru, v. 25, n. 6, p. 596-603, 2017. 1678-7757 http://hdl.handle.net/11449/163569 10.1590/1678-7757-2016-0513 S1678-77572017000600596 WOS:000417175900003 S1678-77572017000600596.pdf |
url |
http://dx.doi.org/10.1590/1678-7757-2016-0513 http://hdl.handle.net/11449/163569 |
identifier_str_mv |
Journal Of Applied Oral Science. Bauru-sp: Univ Sao Paulo Fac Odontologia Bauru, v. 25, n. 6, p. 596-603, 2017. 1678-7757 10.1590/1678-7757-2016-0513 S1678-77572017000600596 WOS:000417175900003 S1678-77572017000600596.pdf |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Journal Of Applied Oral Science 0,645 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
596-603 application/pdf |
dc.publisher.none.fl_str_mv |
Univ Sao Paulo Fac Odontologia Bauru |
publisher.none.fl_str_mv |
Univ Sao Paulo Fac Odontologia Bauru |
dc.source.none.fl_str_mv |
Web of Science reponame:Repositório Institucional da UNESP instname:Universidade Estadual Paulista (UNESP) instacron:UNESP |
instname_str |
Universidade Estadual Paulista (UNESP) |
instacron_str |
UNESP |
institution |
UNESP |
reponame_str |
Repositório Institucional da UNESP |
collection |
Repositório Institucional da UNESP |
repository.name.fl_str_mv |
Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP) |
repository.mail.fl_str_mv |
repositoriounesp@unesp.br |
_version_ |
1822179118549041152 |
dc.identifier.doi.none.fl_str_mv |
10.1590/1678-7757-2016-0513 |