In vitro effect of amorphous calcium phosphate paste applied for extended periods of time on enamel remineralization
Autor(a) principal: | |
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Data de Publicação: | 2017 |
Outros Autores: | , , , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Journal of applied oral science (Online) |
Texto Completo: | http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1678-77572017000600596 |
Resumo: | Abstract Dental applications based on the unique characteristics of amorphous calcium phosphate stabilized by casein phosphopeptides (CPP-ACP) have been proposed, as well as the improvement of its properties. Objectives: The objective of this study was to determine the ability of topically applied CPP-ACP from a commercial product to remineralize subsurface lesions when applied for extended periods of time (3 h and 8 h). Material and Methods: Artificially induced carious lesions were produced in 50 bovine enamel blocks previously selected by surface hardness. After treatments with gel without F and CPP-ACP applied for 1 minute (Placebo); 2% NaF neutral gel applied for 1 minute (Fluoride 1 min); CPP-ACP applied for 3 min (ACP 3 min); and CPP-ACP applied for 3 h (ACP 3 h) and for 8 h (ACP 8 h), the enamel blocks were submitted to the remineralization pH-cycling. Surface hardness and synchrotron micro-tomography were used to determine the percentage of surface hardness recovery (%SHR) and to calculate mineral concentration (gHAp.cm−3), respectively. The data were submitted to ANOVA followed by the Student-Newman-Keuls test (p<0.05). Results: Fluoride gel presented higher %SHR followed by ACP 3 min (p<0.001). No difference (p = 0.148) was found for Placebo, ACP 3 h and ACP 8 h groups for %SHR. Fluoride gel showed greater mineral concentration (p<0.001) when compared with the other groups. ACP 3 min demonstrated a significant difference (p<0.001) from ACP 3 h and ACP 8 h. The ACP 3 h and 8 h presented a subsurface lesion with development of laminations in all blocks. Conclusion: In this in vitro study the use of CPP-ACP for extended periods of time did not produce an additive effect in the remineralization process. |
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Journal of applied oral science (Online) |
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In vitro effect of amorphous calcium phosphate paste applied for extended periods of time on enamel remineralizationX-ray microtomographyTooth remineralizationCalcium phosphatesFluorideDental enamelAbstract Dental applications based on the unique characteristics of amorphous calcium phosphate stabilized by casein phosphopeptides (CPP-ACP) have been proposed, as well as the improvement of its properties. Objectives: The objective of this study was to determine the ability of topically applied CPP-ACP from a commercial product to remineralize subsurface lesions when applied for extended periods of time (3 h and 8 h). Material and Methods: Artificially induced carious lesions were produced in 50 bovine enamel blocks previously selected by surface hardness. After treatments with gel without F and CPP-ACP applied for 1 minute (Placebo); 2% NaF neutral gel applied for 1 minute (Fluoride 1 min); CPP-ACP applied for 3 min (ACP 3 min); and CPP-ACP applied for 3 h (ACP 3 h) and for 8 h (ACP 8 h), the enamel blocks were submitted to the remineralization pH-cycling. Surface hardness and synchrotron micro-tomography were used to determine the percentage of surface hardness recovery (%SHR) and to calculate mineral concentration (gHAp.cm−3), respectively. The data were submitted to ANOVA followed by the Student-Newman-Keuls test (p<0.05). Results: Fluoride gel presented higher %SHR followed by ACP 3 min (p<0.001). No difference (p = 0.148) was found for Placebo, ACP 3 h and ACP 8 h groups for %SHR. Fluoride gel showed greater mineral concentration (p<0.001) when compared with the other groups. ACP 3 min demonstrated a significant difference (p<0.001) from ACP 3 h and ACP 8 h. The ACP 3 h and 8 h presented a subsurface lesion with development of laminations in all blocks. Conclusion: In this in vitro study the use of CPP-ACP for extended periods of time did not produce an additive effect in the remineralization process.Faculdade De Odontologia De Bauru - USP2017-12-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersiontext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S1678-77572017000600596Journal of Applied Oral Science v.25 n.6 2017reponame:Journal of applied oral science (Online)instname:Universidade de São Paulo (USP)instacron:USP10.1590/1678-7757-2016-0513info:eu-repo/semantics/openAccessVieira,Ana Elisa de MelloDanelon,MarcelleCamara,Danielle Mendes daRosselli,Eliana RodriguesStock,Stuart RCannon,Mark LXiao,XianghuiDe Carlo,FrancescoDelbem,Alberto Carlos Botazzoeng2017-11-27T00:00:00Zoai:scielo:S1678-77572017000600596Revistahttp://www.scielo.br/jaosPUBhttps://old.scielo.br/oai/scielo-oai.php||jaos@usp.br1678-77651678-7757opendoar:2017-11-27T00:00Journal of applied oral science (Online) - Universidade de São Paulo (USP)false |
dc.title.none.fl_str_mv |
In vitro effect of amorphous calcium phosphate paste applied for extended periods of time on enamel remineralization |
title |
In vitro effect of amorphous calcium phosphate paste applied for extended periods of time on enamel remineralization |
spellingShingle |
In vitro effect of amorphous calcium phosphate paste applied for extended periods of time on enamel remineralization Vieira,Ana Elisa de Mello X-ray microtomography Tooth remineralization Calcium phosphates Fluoride Dental enamel |
title_short |
In vitro effect of amorphous calcium phosphate paste applied for extended periods of time on enamel remineralization |
title_full |
In vitro effect of amorphous calcium phosphate paste applied for extended periods of time on enamel remineralization |
title_fullStr |
In vitro effect of amorphous calcium phosphate paste applied for extended periods of time on enamel remineralization |
title_full_unstemmed |
In vitro effect of amorphous calcium phosphate paste applied for extended periods of time on enamel remineralization |
title_sort |
In vitro effect of amorphous calcium phosphate paste applied for extended periods of time on enamel remineralization |
author |
Vieira,Ana Elisa de Mello |
author_facet |
Vieira,Ana Elisa de Mello Danelon,Marcelle Camara,Danielle Mendes da Rosselli,Eliana Rodrigues Stock,Stuart R Cannon,Mark L Xiao,Xianghui De Carlo,Francesco Delbem,Alberto Carlos Botazzo |
author_role |
author |
author2 |
Danelon,Marcelle Camara,Danielle Mendes da Rosselli,Eliana Rodrigues Stock,Stuart R Cannon,Mark L Xiao,Xianghui De Carlo,Francesco Delbem,Alberto Carlos Botazzo |
author2_role |
author author author author author author author author |
dc.contributor.author.fl_str_mv |
Vieira,Ana Elisa de Mello Danelon,Marcelle Camara,Danielle Mendes da Rosselli,Eliana Rodrigues Stock,Stuart R Cannon,Mark L Xiao,Xianghui De Carlo,Francesco Delbem,Alberto Carlos Botazzo |
dc.subject.por.fl_str_mv |
X-ray microtomography Tooth remineralization Calcium phosphates Fluoride Dental enamel |
topic |
X-ray microtomography Tooth remineralization Calcium phosphates Fluoride Dental enamel |
description |
Abstract Dental applications based on the unique characteristics of amorphous calcium phosphate stabilized by casein phosphopeptides (CPP-ACP) have been proposed, as well as the improvement of its properties. Objectives: The objective of this study was to determine the ability of topically applied CPP-ACP from a commercial product to remineralize subsurface lesions when applied for extended periods of time (3 h and 8 h). Material and Methods: Artificially induced carious lesions were produced in 50 bovine enamel blocks previously selected by surface hardness. After treatments with gel without F and CPP-ACP applied for 1 minute (Placebo); 2% NaF neutral gel applied for 1 minute (Fluoride 1 min); CPP-ACP applied for 3 min (ACP 3 min); and CPP-ACP applied for 3 h (ACP 3 h) and for 8 h (ACP 8 h), the enamel blocks were submitted to the remineralization pH-cycling. Surface hardness and synchrotron micro-tomography were used to determine the percentage of surface hardness recovery (%SHR) and to calculate mineral concentration (gHAp.cm−3), respectively. The data were submitted to ANOVA followed by the Student-Newman-Keuls test (p<0.05). Results: Fluoride gel presented higher %SHR followed by ACP 3 min (p<0.001). No difference (p = 0.148) was found for Placebo, ACP 3 h and ACP 8 h groups for %SHR. Fluoride gel showed greater mineral concentration (p<0.001) when compared with the other groups. ACP 3 min demonstrated a significant difference (p<0.001) from ACP 3 h and ACP 8 h. The ACP 3 h and 8 h presented a subsurface lesion with development of laminations in all blocks. Conclusion: In this in vitro study the use of CPP-ACP for extended periods of time did not produce an additive effect in the remineralization process. |
publishDate |
2017 |
dc.date.none.fl_str_mv |
2017-12-01 |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1678-77572017000600596 |
url |
http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1678-77572017000600596 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
10.1590/1678-7757-2016-0513 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
text/html |
dc.publisher.none.fl_str_mv |
Faculdade De Odontologia De Bauru - USP |
publisher.none.fl_str_mv |
Faculdade De Odontologia De Bauru - USP |
dc.source.none.fl_str_mv |
Journal of Applied Oral Science v.25 n.6 2017 reponame:Journal of applied oral science (Online) instname:Universidade de São Paulo (USP) instacron:USP |
instname_str |
Universidade de São Paulo (USP) |
instacron_str |
USP |
institution |
USP |
reponame_str |
Journal of applied oral science (Online) |
collection |
Journal of applied oral science (Online) |
repository.name.fl_str_mv |
Journal of applied oral science (Online) - Universidade de São Paulo (USP) |
repository.mail.fl_str_mv |
||jaos@usp.br |
_version_ |
1748936439487266816 |