In vitro effect of amorphous calcium phosphate paste applied for extended periods of time on enamel remineralization

• Autor(a) principal: Vieira, Ana Elisa de Mello
• Data de Publicação: 2017
• Outros Autores: Danelon, Marcelle, Camara, Danielle Mendes da, Rosselli, Eliana Rodrigues, Stock, Stuart R, Cannon, Mark L, Xiao, Xianghui, De Carlo, Francesco, Delbem, Alberto Carlos Botazzo
• Tipo de documento: Artigo
• Idioma: eng
• Título da fonte: Journal of applied oral science (Online)
• Texto Completo: https://www.revistas.usp.br/jaos/article/view/142418
• topic: X-ray microtomography; Tooth remineralization; Calcium phosphates; Fluoride; Dental enamel

Dental applications based on the unique characteristics of amorphous calcium phosphate stabilized by casein phosphopeptides (CPP-ACP) have been proposed, as well as the improvement of its properties. Objectives: The objective of this study was to determine the ability of topically applied CPP-ACP from a commercial product to remineralize subsurface lesions when applied for extended periods of time (3 h and 8 h). Material and Methods: Artificially induced carious lesions were produced in 50 bovine enamel blocks previously selected by surface hardness. After treatments with gel without F and CPP-ACP applied for 1 minute (Placebo); 2% NaF neutral gel applied for 1 minute (Fluoride 1 min); CPP-ACP applied for 3 min (ACP 3 min); and CPP-ACP applied for 3 h (ACP 3 h) and for 8 h (ACP 8 h), the enamel blocks were submitted to the remineralization pH-cycling. Surface hardness and synchrotron micro-tomography were used to determine the percentage of surface hardness recovery (%SHR) and to calculate mineral concentration (gHAp.cm−3), respectively. The data were submitted to ANOVA followed by the Student-Newman-Keuls test (p