Annexin A1 peptide is able to induce an anti-parasitic effect in human placental explants infected by Toxoplasma gondii

Detalhes bibliográficos
Autor(a) principal: de Oliveira Cardoso, Marystela Fávero
Data de Publicação: 2018
Outros Autores: Moreli, Jusciele Brogin, Gomes, Angélica Oliveira, de Freitas Zanon, Caroline [UNESP], Silva, Ana Elizabete [UNESP], Paulesu, Luana Ricci, Ietta, Francesca, Mineo, José Roberto, Ferro, Eloísa Amália, Oliani, Sonia Maria [UNESP]
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da UNESP
Texto Completo: http://dx.doi.org/10.1016/j.micpath.2018.07.005
http://hdl.handle.net/11449/176576
Resumo: This study was conducted to investigate annexin A1 (ANXA1) functions in human placental explants infected with Toxoplasma gondii (T. gondii). We examined the first and third trimester placental explants infected with T. gondii (n = 7 placentas/group) to identify the number and location of parasites, ANXA1 protein, potential involvement of formyl peptide receptors (FPR1 and FPR2), and COX-2 expressions by immunohistochemistry. Treatments with Ac2-26 mimetic peptide of ANXA1 were performed to verify the parasitism rate (β-galactosidase assay), prostaglandin E2 levels (ELISA assay), and ANXA1, FPR1 and COX-2 expression in third trimester placentas. Placental explants of third trimester expressed less ANXA1 and were more permissive to T. gondii infection than first trimester placentas that expressed more ANXA1. Ac2-26 treatment increases endogenous ANXA1 and decreases parasitism rate, COX-2, and prostaglandin E2 levels. Altogether, these data provide further insight into the anti-parasitic and anti-inflammatory effects of ANXA1 in placentas infected with T. gondii.
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spelling Annexin A1 peptide is able to induce an anti-parasitic effect in human placental explants infected by Toxoplasma gondiiAc2-26 peptideAnnexin A1PlacentaToxoplasmosisThis study was conducted to investigate annexin A1 (ANXA1) functions in human placental explants infected with Toxoplasma gondii (T. gondii). We examined the first and third trimester placental explants infected with T. gondii (n = 7 placentas/group) to identify the number and location of parasites, ANXA1 protein, potential involvement of formyl peptide receptors (FPR1 and FPR2), and COX-2 expressions by immunohistochemistry. Treatments with Ac2-26 mimetic peptide of ANXA1 were performed to verify the parasitism rate (β-galactosidase assay), prostaglandin E2 levels (ELISA assay), and ANXA1, FPR1 and COX-2 expression in third trimester placentas. Placental explants of third trimester expressed less ANXA1 and were more permissive to T. gondii infection than first trimester placentas that expressed more ANXA1. Ac2-26 treatment increases endogenous ANXA1 and decreases parasitism rate, COX-2, and prostaglandin E2 levels. Altogether, these data provide further insight into the anti-parasitic and anti-inflammatory effects of ANXA1 in placentas infected with T. gondii.Post-Graduation in Structural and Functional Biology Federal University of São Paulo (UNIFESP)Faceres School of MedicineDepartment of Structural Biology Institute of Biological and Natural Sciences Federal University of Triângulo Mineiro (UFTM)Department of Biology Instituto de Biociências Letras e Ciências Exatas São Paulo State University (UNESP)Department of Life Sciences University of SienaDepartment of Immunology Microbiology and Parasitology Federal University of Uberlândia (UFU)Department of Histology and Embryology Federal University of Uberlândia (UFU)Department of Biology Instituto de Biociências Letras e Ciências Exatas São Paulo State University (UNESP)Universidade de São Paulo (USP)Faceres School of MedicineFederal University of Triângulo Mineiro (UFTM)Universidade Estadual Paulista (Unesp)University of SienaUniversidade Federal de Uberlândia (UFU)de Oliveira Cardoso, Marystela FáveroMoreli, Jusciele BroginGomes, Angélica Oliveirade Freitas Zanon, Caroline [UNESP]Silva, Ana Elizabete [UNESP]Paulesu, Luana RicciIetta, FrancescaMineo, José RobertoFerro, Eloísa AmáliaOliani, Sonia Maria [UNESP]2018-12-11T17:21:25Z2018-12-11T17:21:25Z2018-10-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article153-161application/pdfhttp://dx.doi.org/10.1016/j.micpath.2018.07.005Microbial Pathogenesis, v. 123, p. 153-161.1096-12080882-4010http://hdl.handle.net/11449/17657610.1016/j.micpath.2018.07.0052-s2.0-850497446502-s2.0-85049744650.pdfScopusreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengMicrobial Pathogenesis0,7510,751info:eu-repo/semantics/openAccess2024-01-11T06:27:04Zoai:repositorio.unesp.br:11449/176576Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462024-08-05T22:40:26.025814Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false
dc.title.none.fl_str_mv Annexin A1 peptide is able to induce an anti-parasitic effect in human placental explants infected by Toxoplasma gondii
title Annexin A1 peptide is able to induce an anti-parasitic effect in human placental explants infected by Toxoplasma gondii
spellingShingle Annexin A1 peptide is able to induce an anti-parasitic effect in human placental explants infected by Toxoplasma gondii
de Oliveira Cardoso, Marystela Fávero
Ac2-26 peptide
Annexin A1
Placenta
Toxoplasmosis
title_short Annexin A1 peptide is able to induce an anti-parasitic effect in human placental explants infected by Toxoplasma gondii
title_full Annexin A1 peptide is able to induce an anti-parasitic effect in human placental explants infected by Toxoplasma gondii
title_fullStr Annexin A1 peptide is able to induce an anti-parasitic effect in human placental explants infected by Toxoplasma gondii
title_full_unstemmed Annexin A1 peptide is able to induce an anti-parasitic effect in human placental explants infected by Toxoplasma gondii
title_sort Annexin A1 peptide is able to induce an anti-parasitic effect in human placental explants infected by Toxoplasma gondii
author de Oliveira Cardoso, Marystela Fávero
author_facet de Oliveira Cardoso, Marystela Fávero
Moreli, Jusciele Brogin
Gomes, Angélica Oliveira
de Freitas Zanon, Caroline [UNESP]
Silva, Ana Elizabete [UNESP]
Paulesu, Luana Ricci
Ietta, Francesca
Mineo, José Roberto
Ferro, Eloísa Amália
Oliani, Sonia Maria [UNESP]
author_role author
author2 Moreli, Jusciele Brogin
Gomes, Angélica Oliveira
de Freitas Zanon, Caroline [UNESP]
Silva, Ana Elizabete [UNESP]
Paulesu, Luana Ricci
Ietta, Francesca
Mineo, José Roberto
Ferro, Eloísa Amália
Oliani, Sonia Maria [UNESP]
author2_role author
author
author
author
author
author
author
author
author
dc.contributor.none.fl_str_mv Universidade de São Paulo (USP)
Faceres School of Medicine
Federal University of Triângulo Mineiro (UFTM)
Universidade Estadual Paulista (Unesp)
University of Siena
Universidade Federal de Uberlândia (UFU)
dc.contributor.author.fl_str_mv de Oliveira Cardoso, Marystela Fávero
Moreli, Jusciele Brogin
Gomes, Angélica Oliveira
de Freitas Zanon, Caroline [UNESP]
Silva, Ana Elizabete [UNESP]
Paulesu, Luana Ricci
Ietta, Francesca
Mineo, José Roberto
Ferro, Eloísa Amália
Oliani, Sonia Maria [UNESP]
dc.subject.por.fl_str_mv Ac2-26 peptide
Annexin A1
Placenta
Toxoplasmosis
topic Ac2-26 peptide
Annexin A1
Placenta
Toxoplasmosis
description This study was conducted to investigate annexin A1 (ANXA1) functions in human placental explants infected with Toxoplasma gondii (T. gondii). We examined the first and third trimester placental explants infected with T. gondii (n = 7 placentas/group) to identify the number and location of parasites, ANXA1 protein, potential involvement of formyl peptide receptors (FPR1 and FPR2), and COX-2 expressions by immunohistochemistry. Treatments with Ac2-26 mimetic peptide of ANXA1 were performed to verify the parasitism rate (β-galactosidase assay), prostaglandin E2 levels (ELISA assay), and ANXA1, FPR1 and COX-2 expression in third trimester placentas. Placental explants of third trimester expressed less ANXA1 and were more permissive to T. gondii infection than first trimester placentas that expressed more ANXA1. Ac2-26 treatment increases endogenous ANXA1 and decreases parasitism rate, COX-2, and prostaglandin E2 levels. Altogether, these data provide further insight into the anti-parasitic and anti-inflammatory effects of ANXA1 in placentas infected with T. gondii.
publishDate 2018
dc.date.none.fl_str_mv 2018-12-11T17:21:25Z
2018-12-11T17:21:25Z
2018-10-01
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://dx.doi.org/10.1016/j.micpath.2018.07.005
Microbial Pathogenesis, v. 123, p. 153-161.
1096-1208
0882-4010
http://hdl.handle.net/11449/176576
10.1016/j.micpath.2018.07.005
2-s2.0-85049744650
2-s2.0-85049744650.pdf
url http://dx.doi.org/10.1016/j.micpath.2018.07.005
http://hdl.handle.net/11449/176576
identifier_str_mv Microbial Pathogenesis, v. 123, p. 153-161.
1096-1208
0882-4010
10.1016/j.micpath.2018.07.005
2-s2.0-85049744650
2-s2.0-85049744650.pdf
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv Microbial Pathogenesis
0,751
0,751
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv 153-161
application/pdf
dc.source.none.fl_str_mv Scopus
reponame:Repositório Institucional da UNESP
instname:Universidade Estadual Paulista (UNESP)
instacron:UNESP
instname_str Universidade Estadual Paulista (UNESP)
instacron_str UNESP
institution UNESP
reponame_str Repositório Institucional da UNESP
collection Repositório Institucional da UNESP
repository.name.fl_str_mv Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)
repository.mail.fl_str_mv
_version_ 1808129450019127296