The ideal holding time for boar semen is 24 h at 17 degrees C prior to short-cryopreservation protocols
Autor(a) principal: | |
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Data de Publicação: | 2019 |
Outros Autores: | , , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UNESP |
Texto Completo: | http://dx.doi.org/10.1016/j.cryobiol.2018.12.004 http://hdl.handle.net/11449/185452 |
Resumo: | Boar semen cannot be immediately cryopreserved, it need be hold at 17 degrees C prior to cryopreservation, holding time has been used to improve cryopreserved boar semen, since holding time allows a prolonged interaction between spermatozoa and seminal plasma components. However, until now only few periods of holding time have been studied, and boar semen had been held at 17 degrees C for 24 h to facilitate its manufacture. Thus, this experiment aims to study the effect several holding time (0, 4, 8, 12, 24, 28 and 32 h) on boar spermatozoa post thawed (PT) characteristics. Fifteen sperm-rich fractions of ejaculate were extended in Beltsville Thawing Solution and storage at 17 degrees C. After each holding time (0, 4, 8, 12, 24, 28 and 32 h), a sample was centrifuged, and sperm pellet was diluted in an extender composed of sugars, amino acids, buffers, 20% egg yolk (v/v), antibiotics, 2% glycerol as a cryoprotectant, and 2% methylformamide (v/v). Cryopreservation was performed with an automatic cryopreservation system. Cryopreserved boar semen was evaluated to spermatozoa kinetics, plasma and acrosomal membranes integrity, mitochondrial membrane potential, detection of superoxide anion, plasma membrane fluidity, and peroxidation. Twenty-four hours of holding increase total and progressive motility, rapid spermatozoa, and integrity of plasma and acrosome membranes. To mitochondrial membrane potential, 32 h is needed. However, holding time was not able to control the superoxide anion amount neither membrane lipid peroxidation, and had no effects on membrane fluidity. Thus, to reach the best results of PT boar semen the ideal holding time is 24 h. |
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The ideal holding time for boar semen is 24 h at 17 degrees C prior to short-cryopreservation protocolsSwineSpermatozoaCryopreservationCoolingMembrane integrityBoar semen cannot be immediately cryopreserved, it need be hold at 17 degrees C prior to cryopreservation, holding time has been used to improve cryopreserved boar semen, since holding time allows a prolonged interaction between spermatozoa and seminal plasma components. However, until now only few periods of holding time have been studied, and boar semen had been held at 17 degrees C for 24 h to facilitate its manufacture. Thus, this experiment aims to study the effect several holding time (0, 4, 8, 12, 24, 28 and 32 h) on boar spermatozoa post thawed (PT) characteristics. Fifteen sperm-rich fractions of ejaculate were extended in Beltsville Thawing Solution and storage at 17 degrees C. After each holding time (0, 4, 8, 12, 24, 28 and 32 h), a sample was centrifuged, and sperm pellet was diluted in an extender composed of sugars, amino acids, buffers, 20% egg yolk (v/v), antibiotics, 2% glycerol as a cryoprotectant, and 2% methylformamide (v/v). Cryopreservation was performed with an automatic cryopreservation system. Cryopreserved boar semen was evaluated to spermatozoa kinetics, plasma and acrosomal membranes integrity, mitochondrial membrane potential, detection of superoxide anion, plasma membrane fluidity, and peroxidation. Twenty-four hours of holding increase total and progressive motility, rapid spermatozoa, and integrity of plasma and acrosome membranes. To mitochondrial membrane potential, 32 h is needed. However, holding time was not able to control the superoxide anion amount neither membrane lipid peroxidation, and had no effects on membrane fluidity. Thus, to reach the best results of PT boar semen the ideal holding time is 24 h.Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)Univ Sao Paulo, Swine Res Ctr, Sch Vet Med & Anim Sci, Pirassununga, SP, BrazilSao Paulo State Univ, Sch Vet Med & Anim Sci, Dept Anim Reprod & Vet Radiol, Botucatu, SP, BrazilSao Paulo State Univ, Sch Vet Med & Anim Sci, Dept Anim Reprod & Vet Radiol, Botucatu, SP, BrazilFAPESP: 2015/17620-7FAPESP: 2016/09441-8FAPESP: 2016/24690-4Elsevier B.V.Universidade de São Paulo (USP)Universidade Estadual Paulista (Unesp)Torres, Mariana A.Monteiro, Matheus S.Passarelli, Marina S.Papa, Frederico O. [UNESP]Dell'Aqua, Jose Antonio [UNESP]Alvarenga, Marco Antonio [UNESP]Martins, Simone M. M. K.Andrade, Andre F. C. de2019-10-04T12:35:36Z2019-10-04T12:35:36Z2019-02-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article58-64http://dx.doi.org/10.1016/j.cryobiol.2018.12.004Cryobiology. San Diego: Academic Press Inc Elsevier Science, v. 86, p. 58-64, 2019.0011-2240http://hdl.handle.net/11449/18545210.1016/j.cryobiol.2018.12.004WOS:0004592374000090473846154288947Web of Sciencereponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengCryobiologyinfo:eu-repo/semantics/openAccess2021-10-23T05:17:32Zoai:repositorio.unesp.br:11449/185452Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462021-10-23T05:17:32Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false |
dc.title.none.fl_str_mv |
The ideal holding time for boar semen is 24 h at 17 degrees C prior to short-cryopreservation protocols |
title |
The ideal holding time for boar semen is 24 h at 17 degrees C prior to short-cryopreservation protocols |
spellingShingle |
The ideal holding time for boar semen is 24 h at 17 degrees C prior to short-cryopreservation protocols Torres, Mariana A. Swine Spermatozoa Cryopreservation Cooling Membrane integrity |
title_short |
The ideal holding time for boar semen is 24 h at 17 degrees C prior to short-cryopreservation protocols |
title_full |
The ideal holding time for boar semen is 24 h at 17 degrees C prior to short-cryopreservation protocols |
title_fullStr |
The ideal holding time for boar semen is 24 h at 17 degrees C prior to short-cryopreservation protocols |
title_full_unstemmed |
The ideal holding time for boar semen is 24 h at 17 degrees C prior to short-cryopreservation protocols |
title_sort |
The ideal holding time for boar semen is 24 h at 17 degrees C prior to short-cryopreservation protocols |
author |
Torres, Mariana A. |
author_facet |
Torres, Mariana A. Monteiro, Matheus S. Passarelli, Marina S. Papa, Frederico O. [UNESP] Dell'Aqua, Jose Antonio [UNESP] Alvarenga, Marco Antonio [UNESP] Martins, Simone M. M. K. Andrade, Andre F. C. de |
author_role |
author |
author2 |
Monteiro, Matheus S. Passarelli, Marina S. Papa, Frederico O. [UNESP] Dell'Aqua, Jose Antonio [UNESP] Alvarenga, Marco Antonio [UNESP] Martins, Simone M. M. K. Andrade, Andre F. C. de |
author2_role |
author author author author author author author |
dc.contributor.none.fl_str_mv |
Universidade de São Paulo (USP) Universidade Estadual Paulista (Unesp) |
dc.contributor.author.fl_str_mv |
Torres, Mariana A. Monteiro, Matheus S. Passarelli, Marina S. Papa, Frederico O. [UNESP] Dell'Aqua, Jose Antonio [UNESP] Alvarenga, Marco Antonio [UNESP] Martins, Simone M. M. K. Andrade, Andre F. C. de |
dc.subject.por.fl_str_mv |
Swine Spermatozoa Cryopreservation Cooling Membrane integrity |
topic |
Swine Spermatozoa Cryopreservation Cooling Membrane integrity |
description |
Boar semen cannot be immediately cryopreserved, it need be hold at 17 degrees C prior to cryopreservation, holding time has been used to improve cryopreserved boar semen, since holding time allows a prolonged interaction between spermatozoa and seminal plasma components. However, until now only few periods of holding time have been studied, and boar semen had been held at 17 degrees C for 24 h to facilitate its manufacture. Thus, this experiment aims to study the effect several holding time (0, 4, 8, 12, 24, 28 and 32 h) on boar spermatozoa post thawed (PT) characteristics. Fifteen sperm-rich fractions of ejaculate were extended in Beltsville Thawing Solution and storage at 17 degrees C. After each holding time (0, 4, 8, 12, 24, 28 and 32 h), a sample was centrifuged, and sperm pellet was diluted in an extender composed of sugars, amino acids, buffers, 20% egg yolk (v/v), antibiotics, 2% glycerol as a cryoprotectant, and 2% methylformamide (v/v). Cryopreservation was performed with an automatic cryopreservation system. Cryopreserved boar semen was evaluated to spermatozoa kinetics, plasma and acrosomal membranes integrity, mitochondrial membrane potential, detection of superoxide anion, plasma membrane fluidity, and peroxidation. Twenty-four hours of holding increase total and progressive motility, rapid spermatozoa, and integrity of plasma and acrosome membranes. To mitochondrial membrane potential, 32 h is needed. However, holding time was not able to control the superoxide anion amount neither membrane lipid peroxidation, and had no effects on membrane fluidity. Thus, to reach the best results of PT boar semen the ideal holding time is 24 h. |
publishDate |
2019 |
dc.date.none.fl_str_mv |
2019-10-04T12:35:36Z 2019-10-04T12:35:36Z 2019-02-01 |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://dx.doi.org/10.1016/j.cryobiol.2018.12.004 Cryobiology. San Diego: Academic Press Inc Elsevier Science, v. 86, p. 58-64, 2019. 0011-2240 http://hdl.handle.net/11449/185452 10.1016/j.cryobiol.2018.12.004 WOS:000459237400009 0473846154288947 |
url |
http://dx.doi.org/10.1016/j.cryobiol.2018.12.004 http://hdl.handle.net/11449/185452 |
identifier_str_mv |
Cryobiology. San Diego: Academic Press Inc Elsevier Science, v. 86, p. 58-64, 2019. 0011-2240 10.1016/j.cryobiol.2018.12.004 WOS:000459237400009 0473846154288947 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Cryobiology |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
58-64 |
dc.publisher.none.fl_str_mv |
Elsevier B.V. |
publisher.none.fl_str_mv |
Elsevier B.V. |
dc.source.none.fl_str_mv |
Web of Science reponame:Repositório Institucional da UNESP instname:Universidade Estadual Paulista (UNESP) instacron:UNESP |
instname_str |
Universidade Estadual Paulista (UNESP) |
instacron_str |
UNESP |
institution |
UNESP |
reponame_str |
Repositório Institucional da UNESP |
collection |
Repositório Institucional da UNESP |
repository.name.fl_str_mv |
Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP) |
repository.mail.fl_str_mv |
|
_version_ |
1803047125700837376 |