Corona protein impacts on alternating current biosusceptometry signal and circulation times of differently coated MnFe2O4nanoparticles

• Autor(a) principal: Prospero, Andre Gonçalves [UNESP]
• Data de Publicação: 2021
• Outros Autores: Buranello, Lais Pereira [UNESP], Fernandes, Carlos Ah [UNESP], Dos Santos, Lucilene Delazari [UNESP], Soares, Guilherme [UNESP], C Rossini, Bruno [UNESP], Zufelato, Nícholas, Bakuzis, Andris Figueiroa, De Mattos Fontes, Marcos R [UNESP], De Arruda Miranda, José R [UNESP]
• Tipo de documento: Artigo
• Idioma: eng
• Título da fonte: Repositório Institucional da UNESP
• Texto Completo: http://dx.doi.org/10.2217/nnm-2021-0195; http://hdl.handle.net/11449/229666
• topic: alternating current biosusceptometry; biomedical engineering; corona protein; magnetic nanoparticles; mass spectrometry

Background: We evaluated the impacts of corona protein (CP) formation on the alternating current biosusceptometry (ACB) signal intensity and in vivo circulation times of three differently coated magnetic nanoparticles (MNP): bare, citrate-coated and bovine serum albumin-coated MNPs. Methods: We employed the ACB system, gel electrophoresis and mass spectrometry analysis. Results: Higher CP formation led to a greater reduction in the in vitro ACB signal intensity and circulation time. We found fewer proteins forming the CP for the bovine serum albumin-coated MNPs, which presented the highest circulation time in vivo among the MNPs studied. Conclusion: These data showed better biocompatibility, stability and magnetic signal uniformity in biological media for bovine serum albumin-coated MNPs than for citrate-coated MNPs and bare MNPs.