Improved laboratory safety by decontamination of unstained sputum smears for acid-fast microscopy
Autor(a) principal: | |
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Data de Publicação: | 2005 |
Outros Autores: | , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UNESP |
Texto Completo: | http://dx.doi.org/10.1128/JCM.43.8.4245-4248.2005 http://hdl.handle.net/11449/7530 |
Resumo: | Tubercle bacilli may survive in unstained heat-fixed sputum smears and may be an infection risk to laboratory staff. We compared the effectiveness of 1% and 5% sodium hypochlorite, 5% phenol, 2% glutaraldehyde, and 3.7% formalin in killing Mycobacterium tuberculosis present in smears prepared from 51 sputum samples. The smears were decontaminated by the tube and slide techniques. Phenol at 5%, glutaraldehyde at 2%, and buffered formalin at 3.7% for 1 min (tube technique) or for 10 min (slide technique) were effective in decontaminating sputum smears and preserved cell morphology and quantitative acid-fast microscopy results. |
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Improved laboratory safety by decontamination of unstained sputum smears for acid-fast microscopyTubercle bacilli may survive in unstained heat-fixed sputum smears and may be an infection risk to laboratory staff. We compared the effectiveness of 1% and 5% sodium hypochlorite, 5% phenol, 2% glutaraldehyde, and 3.7% formalin in killing Mycobacterium tuberculosis present in smears prepared from 51 sputum samples. The smears were decontaminated by the tube and slide techniques. Phenol at 5%, glutaraldehyde at 2%, and buffered formalin at 3.7% for 1 min (tube technique) or for 10 min (slide technique) were effective in decontaminating sputum smears and preserved cell morphology and quantitative acid-fast microscopy results.Ctr Referencia Prof Helio Fraga, Rio de Janeiro, BrazilUniv Estadual Maringa, Dept Anal Clin, Maringa, Parana, BrazilUNESP, Fac Ciências Farmaceut, Araraquara, SP, BrazilUNESP, Fac Ciências Farmaceut, Araraquara, SP, BrazilAmer Soc MicrobiologyCtr Referencia Prof Helio FragaUniversidade Estadual de Maringá (UEM)Universidade Estadual Paulista (Unesp)Giacomelli, LRBHelbel, C.Ogassawara, RLNBarreto, AMWMartins, F. M.Cardoso, C. L.Leite, CQF2014-05-20T13:24:22Z2014-05-20T13:24:22Z2005-08-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article4245-4248application/pdfhttp://dx.doi.org/10.1128/JCM.43.8.4245-4248.2005Journal of Clinical Microbiology. Washington: Amer Soc Microbiology, v. 43, n. 8, p. 4245-4248, 2005.0095-1137http://hdl.handle.net/11449/753010.1128/JCM.43.8.4245-4248.2005WOS:000231136800107WOS000231136800107.pdfWeb of Sciencereponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengJournal of Clinical Microbiology4.0542,256info:eu-repo/semantics/openAccess2024-01-02T06:17:57Zoai:repositorio.unesp.br:11449/7530Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462024-01-02T06:17:57Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false |
dc.title.none.fl_str_mv |
Improved laboratory safety by decontamination of unstained sputum smears for acid-fast microscopy |
title |
Improved laboratory safety by decontamination of unstained sputum smears for acid-fast microscopy |
spellingShingle |
Improved laboratory safety by decontamination of unstained sputum smears for acid-fast microscopy Giacomelli, LRB |
title_short |
Improved laboratory safety by decontamination of unstained sputum smears for acid-fast microscopy |
title_full |
Improved laboratory safety by decontamination of unstained sputum smears for acid-fast microscopy |
title_fullStr |
Improved laboratory safety by decontamination of unstained sputum smears for acid-fast microscopy |
title_full_unstemmed |
Improved laboratory safety by decontamination of unstained sputum smears for acid-fast microscopy |
title_sort |
Improved laboratory safety by decontamination of unstained sputum smears for acid-fast microscopy |
author |
Giacomelli, LRB |
author_facet |
Giacomelli, LRB Helbel, C. Ogassawara, RLN Barreto, AMW Martins, F. M. Cardoso, C. L. Leite, CQF |
author_role |
author |
author2 |
Helbel, C. Ogassawara, RLN Barreto, AMW Martins, F. M. Cardoso, C. L. Leite, CQF |
author2_role |
author author author author author author |
dc.contributor.none.fl_str_mv |
Ctr Referencia Prof Helio Fraga Universidade Estadual de Maringá (UEM) Universidade Estadual Paulista (Unesp) |
dc.contributor.author.fl_str_mv |
Giacomelli, LRB Helbel, C. Ogassawara, RLN Barreto, AMW Martins, F. M. Cardoso, C. L. Leite, CQF |
description |
Tubercle bacilli may survive in unstained heat-fixed sputum smears and may be an infection risk to laboratory staff. We compared the effectiveness of 1% and 5% sodium hypochlorite, 5% phenol, 2% glutaraldehyde, and 3.7% formalin in killing Mycobacterium tuberculosis present in smears prepared from 51 sputum samples. The smears were decontaminated by the tube and slide techniques. Phenol at 5%, glutaraldehyde at 2%, and buffered formalin at 3.7% for 1 min (tube technique) or for 10 min (slide technique) were effective in decontaminating sputum smears and preserved cell morphology and quantitative acid-fast microscopy results. |
publishDate |
2005 |
dc.date.none.fl_str_mv |
2005-08-01 2014-05-20T13:24:22Z 2014-05-20T13:24:22Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://dx.doi.org/10.1128/JCM.43.8.4245-4248.2005 Journal of Clinical Microbiology. Washington: Amer Soc Microbiology, v. 43, n. 8, p. 4245-4248, 2005. 0095-1137 http://hdl.handle.net/11449/7530 10.1128/JCM.43.8.4245-4248.2005 WOS:000231136800107 WOS000231136800107.pdf |
url |
http://dx.doi.org/10.1128/JCM.43.8.4245-4248.2005 http://hdl.handle.net/11449/7530 |
identifier_str_mv |
Journal of Clinical Microbiology. Washington: Amer Soc Microbiology, v. 43, n. 8, p. 4245-4248, 2005. 0095-1137 10.1128/JCM.43.8.4245-4248.2005 WOS:000231136800107 WOS000231136800107.pdf |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Journal of Clinical Microbiology 4.054 2,256 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
4245-4248 application/pdf |
dc.publisher.none.fl_str_mv |
Amer Soc Microbiology |
publisher.none.fl_str_mv |
Amer Soc Microbiology |
dc.source.none.fl_str_mv |
Web of Science reponame:Repositório Institucional da UNESP instname:Universidade Estadual Paulista (UNESP) instacron:UNESP |
instname_str |
Universidade Estadual Paulista (UNESP) |
instacron_str |
UNESP |
institution |
UNESP |
reponame_str |
Repositório Institucional da UNESP |
collection |
Repositório Institucional da UNESP |
repository.name.fl_str_mv |
Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP) |
repository.mail.fl_str_mv |
|
_version_ |
1803047223772053504 |