Sustainable one-pot platform for the green recovery of carotenoids from Phaffia rhodozyma yeast and their use as natural additives in soap formulation

Detalhes bibliográficos
Autor(a) principal: Mussagy, Cassamo U.
Data de Publicação: 2023
Outros Autores: Farias, Fabiane O., Santos-Ebinuma, Valeria C. [UNESP], Pereira, Jorge F.B., Pessoa, Adalberto
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da UNESP
Texto Completo: http://dx.doi.org/10.1016/j.eti.2023.103029
http://hdl.handle.net/11449/246759
Resumo: Microbial-based pigments have attracted the interest of the cleaning and cosmeceutical industries as greener and more effective alternatives to the synthetic antioxidants and antimicrobials used in commercially colored soaps. Astaxanthin and β-carotene are natural carotenoids with strong antioxidant activity produced by yeast Phaffia rhodozyma (P. rhodozyma), but its use in industrial-scale processes is still very scarce. The optimization of downstream processing operations for the recovery of carotenoids from P. rhodozyma using environmentally and energetically favorable processes has not yet been fully achieved. In the present work, we propose a simple and sustainable one-pot process using biocompatible and renewable solvents (ethanolic carboxylic acids mixtures) for the recovery of astaxanthin and β-carotene from P. rhodozyma biomass and direct formulation of bioactive soaps, thus minimizing the processing units. For this purpose, carotenoids-enriched ethanolic carboxylic acids solutions were directly (“one-pot”) used in the following saponification process for the formulation of bioactive soaps without the need for additional purification units. The recovery aptitude of ethanolic carboxylic acids solutions and the corresponding solvation mechanisms of different solvent mixtures for astaxanthin and β-carotene were screened using the COSMO-SAC model. Operating parameters (e.g., solvent ratio, time extraction, temperature, and re-extraction cycles) were optimized for the solvent mixture (ethanol:lauric acid) that exhibited the highest recovery capacity for both carotenoids. The incorporation of microbial carotenoids in formulated soaps decreases the moisture content (from 10.04 to 6.85%), the pH (from 8.83 to 8.68), and the surface hardness (from 0.68 to 0.60 mm) and increase the foam height (from 5.83 to 7.07 cm). The biological activity of carotenoids-rich soaps was confirmed by antioxidant tests (60.53%). This work demonstrated that mixing natural yeast-based carotenoids with biosolvents is a simple, effective and sustainable solution for the formulation of functional and colored soaps.
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spelling Sustainable one-pot platform for the green recovery of carotenoids from Phaffia rhodozyma yeast and their use as natural additives in soap formulationAstaxanthinCarboxylic acidsNatural antioxidantsPhaffia rhodozymaSoap formulationβ-caroteneMicrobial-based pigments have attracted the interest of the cleaning and cosmeceutical industries as greener and more effective alternatives to the synthetic antioxidants and antimicrobials used in commercially colored soaps. Astaxanthin and β-carotene are natural carotenoids with strong antioxidant activity produced by yeast Phaffia rhodozyma (P. rhodozyma), but its use in industrial-scale processes is still very scarce. The optimization of downstream processing operations for the recovery of carotenoids from P. rhodozyma using environmentally and energetically favorable processes has not yet been fully achieved. In the present work, we propose a simple and sustainable one-pot process using biocompatible and renewable solvents (ethanolic carboxylic acids mixtures) for the recovery of astaxanthin and β-carotene from P. rhodozyma biomass and direct formulation of bioactive soaps, thus minimizing the processing units. For this purpose, carotenoids-enriched ethanolic carboxylic acids solutions were directly (“one-pot”) used in the following saponification process for the formulation of bioactive soaps without the need for additional purification units. The recovery aptitude of ethanolic carboxylic acids solutions and the corresponding solvation mechanisms of different solvent mixtures for astaxanthin and β-carotene were screened using the COSMO-SAC model. Operating parameters (e.g., solvent ratio, time extraction, temperature, and re-extraction cycles) were optimized for the solvent mixture (ethanol:lauric acid) that exhibited the highest recovery capacity for both carotenoids. The incorporation of microbial carotenoids in formulated soaps decreases the moisture content (from 10.04 to 6.85%), the pH (from 8.83 to 8.68), and the surface hardness (from 0.68 to 0.60 mm) and increase the foam height (from 5.83 to 7.07 cm). The biological activity of carotenoids-rich soaps was confirmed by antioxidant tests (60.53%). This work demonstrated that mixing natural yeast-based carotenoids with biosolvents is a simple, effective and sustainable solution for the formulation of functional and colored soaps.Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)Escuela de Agronomía Facultad de Ciencias Agronómicas y de los Alimentos Pontificia Universidad Católica de ValparaísoDepartment of Chemical Engineering Federal University of ParanáDepartment of Engineering of Bioprocesses and Biotechnology School of Pharmaceutical Sciences São Paulo State University (UNESP) AraraquaraUniv Coimbra CIEPQPF Department of Chemical Engineering, Rua Sílvio Lima, Pólo II – Pinhal de MarrocosDepartment of Pharmaceutical-Biochemical Technology School of Pharmaceutical Sciences University of São PauloDepartment of Engineering of Bioprocesses and Biotechnology School of Pharmaceutical Sciences São Paulo State University (UNESP) AraraquaraCNPq: 312463/2021-9Pontificia Universidad Católica de ValparaísoFederal University of ParanáUniversidade Estadual Paulista (UNESP)CIEPQPFUniversidade de São Paulo (USP)Mussagy, Cassamo U.Farias, Fabiane O.Santos-Ebinuma, Valeria C. [UNESP]Pereira, Jorge F.B.Pessoa, Adalberto2023-07-29T12:49:47Z2023-07-29T12:49:47Z2023-02-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articlehttp://dx.doi.org/10.1016/j.eti.2023.103029Environmental Technology and Innovation, v. 29.2352-1864http://hdl.handle.net/11449/24675910.1016/j.eti.2023.1030292-s2.0-85147327652Scopusreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengEnvironmental Technology and Innovationinfo:eu-repo/semantics/openAccess2023-07-29T12:49:47Zoai:repositorio.unesp.br:11449/246759Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462024-08-05T15:47:57.047466Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false
dc.title.none.fl_str_mv Sustainable one-pot platform for the green recovery of carotenoids from Phaffia rhodozyma yeast and their use as natural additives in soap formulation
title Sustainable one-pot platform for the green recovery of carotenoids from Phaffia rhodozyma yeast and their use as natural additives in soap formulation
spellingShingle Sustainable one-pot platform for the green recovery of carotenoids from Phaffia rhodozyma yeast and their use as natural additives in soap formulation
Mussagy, Cassamo U.
Astaxanthin
Carboxylic acids
Natural antioxidants
Phaffia rhodozyma
Soap formulation
β-carotene
title_short Sustainable one-pot platform for the green recovery of carotenoids from Phaffia rhodozyma yeast and their use as natural additives in soap formulation
title_full Sustainable one-pot platform for the green recovery of carotenoids from Phaffia rhodozyma yeast and their use as natural additives in soap formulation
title_fullStr Sustainable one-pot platform for the green recovery of carotenoids from Phaffia rhodozyma yeast and their use as natural additives in soap formulation
title_full_unstemmed Sustainable one-pot platform for the green recovery of carotenoids from Phaffia rhodozyma yeast and their use as natural additives in soap formulation
title_sort Sustainable one-pot platform for the green recovery of carotenoids from Phaffia rhodozyma yeast and their use as natural additives in soap formulation
author Mussagy, Cassamo U.
author_facet Mussagy, Cassamo U.
Farias, Fabiane O.
Santos-Ebinuma, Valeria C. [UNESP]
Pereira, Jorge F.B.
Pessoa, Adalberto
author_role author
author2 Farias, Fabiane O.
Santos-Ebinuma, Valeria C. [UNESP]
Pereira, Jorge F.B.
Pessoa, Adalberto
author2_role author
author
author
author
dc.contributor.none.fl_str_mv Pontificia Universidad Católica de Valparaíso
Federal University of Paraná
Universidade Estadual Paulista (UNESP)
CIEPQPF
Universidade de São Paulo (USP)
dc.contributor.author.fl_str_mv Mussagy, Cassamo U.
Farias, Fabiane O.
Santos-Ebinuma, Valeria C. [UNESP]
Pereira, Jorge F.B.
Pessoa, Adalberto
dc.subject.por.fl_str_mv Astaxanthin
Carboxylic acids
Natural antioxidants
Phaffia rhodozyma
Soap formulation
β-carotene
topic Astaxanthin
Carboxylic acids
Natural antioxidants
Phaffia rhodozyma
Soap formulation
β-carotene
description Microbial-based pigments have attracted the interest of the cleaning and cosmeceutical industries as greener and more effective alternatives to the synthetic antioxidants and antimicrobials used in commercially colored soaps. Astaxanthin and β-carotene are natural carotenoids with strong antioxidant activity produced by yeast Phaffia rhodozyma (P. rhodozyma), but its use in industrial-scale processes is still very scarce. The optimization of downstream processing operations for the recovery of carotenoids from P. rhodozyma using environmentally and energetically favorable processes has not yet been fully achieved. In the present work, we propose a simple and sustainable one-pot process using biocompatible and renewable solvents (ethanolic carboxylic acids mixtures) for the recovery of astaxanthin and β-carotene from P. rhodozyma biomass and direct formulation of bioactive soaps, thus minimizing the processing units. For this purpose, carotenoids-enriched ethanolic carboxylic acids solutions were directly (“one-pot”) used in the following saponification process for the formulation of bioactive soaps without the need for additional purification units. The recovery aptitude of ethanolic carboxylic acids solutions and the corresponding solvation mechanisms of different solvent mixtures for astaxanthin and β-carotene were screened using the COSMO-SAC model. Operating parameters (e.g., solvent ratio, time extraction, temperature, and re-extraction cycles) were optimized for the solvent mixture (ethanol:lauric acid) that exhibited the highest recovery capacity for both carotenoids. The incorporation of microbial carotenoids in formulated soaps decreases the moisture content (from 10.04 to 6.85%), the pH (from 8.83 to 8.68), and the surface hardness (from 0.68 to 0.60 mm) and increase the foam height (from 5.83 to 7.07 cm). The biological activity of carotenoids-rich soaps was confirmed by antioxidant tests (60.53%). This work demonstrated that mixing natural yeast-based carotenoids with biosolvents is a simple, effective and sustainable solution for the formulation of functional and colored soaps.
publishDate 2023
dc.date.none.fl_str_mv 2023-07-29T12:49:47Z
2023-07-29T12:49:47Z
2023-02-01
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://dx.doi.org/10.1016/j.eti.2023.103029
Environmental Technology and Innovation, v. 29.
2352-1864
http://hdl.handle.net/11449/246759
10.1016/j.eti.2023.103029
2-s2.0-85147327652
url http://dx.doi.org/10.1016/j.eti.2023.103029
http://hdl.handle.net/11449/246759
identifier_str_mv Environmental Technology and Innovation, v. 29.
2352-1864
10.1016/j.eti.2023.103029
2-s2.0-85147327652
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv Environmental Technology and Innovation
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.source.none.fl_str_mv Scopus
reponame:Repositório Institucional da UNESP
instname:Universidade Estadual Paulista (UNESP)
instacron:UNESP
instname_str Universidade Estadual Paulista (UNESP)
instacron_str UNESP
institution UNESP
reponame_str Repositório Institucional da UNESP
collection Repositório Institucional da UNESP
repository.name.fl_str_mv Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)
repository.mail.fl_str_mv
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