Analysis of Nucleotide Alterations in the E6 Genomic Region of Human Papillomavirus Types 6 and 11 in Condyloma Acuminatum Samples from Brazil
Autor(a) principal: | |
---|---|
Data de Publicação: | 2019 |
Outros Autores: | , , , , , , , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UNESP |
Texto Completo: | http://dx.doi.org/10.1155/2019/5697573 http://hdl.handle.net/11449/186373 |
Resumo: | Condyloma acuminata (CA), or genital warts, are benign proliferative epidermal or mucous lesions that are caused by infection with human papillomavirus (HPV), mainly the low-risk types 6 and 11. HPV variants are defined as viral sequences that share identity in the nucleotide sequence of the L1 gene greater than 98%. Based on this criterion, HPV6 and 11 variant lineages have been studied, and there are ongoing attempts to correlate these genetic variants with different clinical findings of infection. Therefore, the aims of this study were to detect variants and nucleotide alterations present in the E6 regions of HPV types 6 and 11 found in CA samples, to correlate the HPV presence with the clinical-pathological data of the patients, and to determine phylogenetic relationships with variants from other places in the world. The E6 regions of 25 HPV6 samples and 7 HPV11 samples from CA were amplified using PCR with specific primers. The products were ligated to a cloning vector and five colonies of each sample were sequenced to observe the nucleotide alterations. Twelve samples were identified as the HPV6B3 variant, presenting the mutation (guanine) G474A (adenine), and one of them also showed the mutation (thymine) T369G. The other 13 patients were positive for HPV6B1 without nucleotide alterations. In the analysis of the HPV11 samples, all patients showed the mutations T137C and (cytosine) C380T. One patient also presented the nucleotide alteration T410C. None of the mutations found in the 32 analyzed samples resulted in amino acid changes. Patient age, local occurrence, and HIV infection did not show significant association with HPV infection. Besides, the data found in this study did not show a relationship with the geographical region of isolation when compared to other data from different regions of the world. In this way, despite the nucleotide alterations found, it was not possible to observe amino acid changes and variants grouping according to geographical region. |
id |
UNSP_bfd9471e16833fcb6f873de7b349cfd5 |
---|---|
oai_identifier_str |
oai:repositorio.unesp.br:11449/186373 |
network_acronym_str |
UNSP |
network_name_str |
Repositório Institucional da UNESP |
repository_id_str |
2946 |
spelling |
Analysis of Nucleotide Alterations in the E6 Genomic Region of Human Papillomavirus Types 6 and 11 in Condyloma Acuminatum Samples from BrazilCondyloma acuminata (CA), or genital warts, are benign proliferative epidermal or mucous lesions that are caused by infection with human papillomavirus (HPV), mainly the low-risk types 6 and 11. HPV variants are defined as viral sequences that share identity in the nucleotide sequence of the L1 gene greater than 98%. Based on this criterion, HPV6 and 11 variant lineages have been studied, and there are ongoing attempts to correlate these genetic variants with different clinical findings of infection. Therefore, the aims of this study were to detect variants and nucleotide alterations present in the E6 regions of HPV types 6 and 11 found in CA samples, to correlate the HPV presence with the clinical-pathological data of the patients, and to determine phylogenetic relationships with variants from other places in the world. The E6 regions of 25 HPV6 samples and 7 HPV11 samples from CA were amplified using PCR with specific primers. The products were ligated to a cloning vector and five colonies of each sample were sequenced to observe the nucleotide alterations. Twelve samples were identified as the HPV6B3 variant, presenting the mutation (guanine) G474A (adenine), and one of them also showed the mutation (thymine) T369G. The other 13 patients were positive for HPV6B1 without nucleotide alterations. In the analysis of the HPV11 samples, all patients showed the mutations T137C and (cytosine) C380T. One patient also presented the nucleotide alteration T410C. None of the mutations found in the 32 analyzed samples resulted in amino acid changes. Patient age, local occurrence, and HIV infection did not show significant association with HPV infection. Besides, the data found in this study did not show a relationship with the geographical region of isolation when compared to other data from different regions of the world. In this way, despite the nucleotide alterations found, it was not possible to observe amino acid changes and variants grouping according to geographical region.Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)Sao Paulo State Univ, Inst Biosci Letters & Exact Sci, Sao Jose Do Rio Preto, SP, BrazilUniv Sao Paulo, Fac Med Ribeirao Preto, Clin Hosp, Ribeirao Preto, SP, BrazilUniv Sao Paulo, Fac Med Ribeirao Preto, Ribeirao Preto, SP, BrazilFac Med Rio Preto, Sao Jose Do Rio Preto, SP, BrazilSao Paulo State Univ, Inst Biosci Letters & Exact Sci, Sao Jose Do Rio Preto, SP, BrazilFAPESP: 2015/06628-7Hindawi LtdUniversidade Estadual Paulista (Unesp)Universidade de São Paulo (USP)Dias, Marina Carrara [UNESP]Stuqui, Bruna [UNESP]Scarin Provazzi, Paola Jocelan [UNESP]Bittar, Cintia [UNESP]Candido, Natalia Maria [UNESP]Adum de Matos, Renata Prandini [UNESP]Badial, Rodolfo Miglioli [UNESP]Bonfim, Caroline Measso do [UNESP]Santos Melli, Patricia Pereira dosQuintana, Silvana MariaCordeiro, Jose AntonioRahal, Paula [UNESP]Calmon, Marilia de Freitas [UNESP]2019-10-04T19:55:01Z2019-10-04T19:55:01Z2019-01-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article10http://dx.doi.org/10.1155/2019/5697573Advances In Virology. London: Hindawi Ltd, 10 p., 2019.1687-8639http://hdl.handle.net/11449/18637310.1155/2019/5697573WOS:000473386900001799108236267121291656014694362400000-0001-5693-6148Web of Sciencereponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengAdvances In Virologyinfo:eu-repo/semantics/openAccess2021-10-22T21:16:04Zoai:repositorio.unesp.br:11449/186373Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462021-10-22T21:16:04Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false |
dc.title.none.fl_str_mv |
Analysis of Nucleotide Alterations in the E6 Genomic Region of Human Papillomavirus Types 6 and 11 in Condyloma Acuminatum Samples from Brazil |
title |
Analysis of Nucleotide Alterations in the E6 Genomic Region of Human Papillomavirus Types 6 and 11 in Condyloma Acuminatum Samples from Brazil |
spellingShingle |
Analysis of Nucleotide Alterations in the E6 Genomic Region of Human Papillomavirus Types 6 and 11 in Condyloma Acuminatum Samples from Brazil Dias, Marina Carrara [UNESP] |
title_short |
Analysis of Nucleotide Alterations in the E6 Genomic Region of Human Papillomavirus Types 6 and 11 in Condyloma Acuminatum Samples from Brazil |
title_full |
Analysis of Nucleotide Alterations in the E6 Genomic Region of Human Papillomavirus Types 6 and 11 in Condyloma Acuminatum Samples from Brazil |
title_fullStr |
Analysis of Nucleotide Alterations in the E6 Genomic Region of Human Papillomavirus Types 6 and 11 in Condyloma Acuminatum Samples from Brazil |
title_full_unstemmed |
Analysis of Nucleotide Alterations in the E6 Genomic Region of Human Papillomavirus Types 6 and 11 in Condyloma Acuminatum Samples from Brazil |
title_sort |
Analysis of Nucleotide Alterations in the E6 Genomic Region of Human Papillomavirus Types 6 and 11 in Condyloma Acuminatum Samples from Brazil |
author |
Dias, Marina Carrara [UNESP] |
author_facet |
Dias, Marina Carrara [UNESP] Stuqui, Bruna [UNESP] Scarin Provazzi, Paola Jocelan [UNESP] Bittar, Cintia [UNESP] Candido, Natalia Maria [UNESP] Adum de Matos, Renata Prandini [UNESP] Badial, Rodolfo Miglioli [UNESP] Bonfim, Caroline Measso do [UNESP] Santos Melli, Patricia Pereira dos Quintana, Silvana Maria Cordeiro, Jose Antonio Rahal, Paula [UNESP] Calmon, Marilia de Freitas [UNESP] |
author_role |
author |
author2 |
Stuqui, Bruna [UNESP] Scarin Provazzi, Paola Jocelan [UNESP] Bittar, Cintia [UNESP] Candido, Natalia Maria [UNESP] Adum de Matos, Renata Prandini [UNESP] Badial, Rodolfo Miglioli [UNESP] Bonfim, Caroline Measso do [UNESP] Santos Melli, Patricia Pereira dos Quintana, Silvana Maria Cordeiro, Jose Antonio Rahal, Paula [UNESP] Calmon, Marilia de Freitas [UNESP] |
author2_role |
author author author author author author author author author author author author |
dc.contributor.none.fl_str_mv |
Universidade Estadual Paulista (Unesp) Universidade de São Paulo (USP) |
dc.contributor.author.fl_str_mv |
Dias, Marina Carrara [UNESP] Stuqui, Bruna [UNESP] Scarin Provazzi, Paola Jocelan [UNESP] Bittar, Cintia [UNESP] Candido, Natalia Maria [UNESP] Adum de Matos, Renata Prandini [UNESP] Badial, Rodolfo Miglioli [UNESP] Bonfim, Caroline Measso do [UNESP] Santos Melli, Patricia Pereira dos Quintana, Silvana Maria Cordeiro, Jose Antonio Rahal, Paula [UNESP] Calmon, Marilia de Freitas [UNESP] |
description |
Condyloma acuminata (CA), or genital warts, are benign proliferative epidermal or mucous lesions that are caused by infection with human papillomavirus (HPV), mainly the low-risk types 6 and 11. HPV variants are defined as viral sequences that share identity in the nucleotide sequence of the L1 gene greater than 98%. Based on this criterion, HPV6 and 11 variant lineages have been studied, and there are ongoing attempts to correlate these genetic variants with different clinical findings of infection. Therefore, the aims of this study were to detect variants and nucleotide alterations present in the E6 regions of HPV types 6 and 11 found in CA samples, to correlate the HPV presence with the clinical-pathological data of the patients, and to determine phylogenetic relationships with variants from other places in the world. The E6 regions of 25 HPV6 samples and 7 HPV11 samples from CA were amplified using PCR with specific primers. The products were ligated to a cloning vector and five colonies of each sample were sequenced to observe the nucleotide alterations. Twelve samples were identified as the HPV6B3 variant, presenting the mutation (guanine) G474A (adenine), and one of them also showed the mutation (thymine) T369G. The other 13 patients were positive for HPV6B1 without nucleotide alterations. In the analysis of the HPV11 samples, all patients showed the mutations T137C and (cytosine) C380T. One patient also presented the nucleotide alteration T410C. None of the mutations found in the 32 analyzed samples resulted in amino acid changes. Patient age, local occurrence, and HIV infection did not show significant association with HPV infection. Besides, the data found in this study did not show a relationship with the geographical region of isolation when compared to other data from different regions of the world. In this way, despite the nucleotide alterations found, it was not possible to observe amino acid changes and variants grouping according to geographical region. |
publishDate |
2019 |
dc.date.none.fl_str_mv |
2019-10-04T19:55:01Z 2019-10-04T19:55:01Z 2019-01-01 |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://dx.doi.org/10.1155/2019/5697573 Advances In Virology. London: Hindawi Ltd, 10 p., 2019. 1687-8639 http://hdl.handle.net/11449/186373 10.1155/2019/5697573 WOS:000473386900001 7991082362671212 9165601469436240 0000-0001-5693-6148 |
url |
http://dx.doi.org/10.1155/2019/5697573 http://hdl.handle.net/11449/186373 |
identifier_str_mv |
Advances In Virology. London: Hindawi Ltd, 10 p., 2019. 1687-8639 10.1155/2019/5697573 WOS:000473386900001 7991082362671212 9165601469436240 0000-0001-5693-6148 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Advances In Virology |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
10 |
dc.publisher.none.fl_str_mv |
Hindawi Ltd |
publisher.none.fl_str_mv |
Hindawi Ltd |
dc.source.none.fl_str_mv |
Web of Science reponame:Repositório Institucional da UNESP instname:Universidade Estadual Paulista (UNESP) instacron:UNESP |
instname_str |
Universidade Estadual Paulista (UNESP) |
instacron_str |
UNESP |
institution |
UNESP |
reponame_str |
Repositório Institucional da UNESP |
collection |
Repositório Institucional da UNESP |
repository.name.fl_str_mv |
Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP) |
repository.mail.fl_str_mv |
|
_version_ |
1803047449120473088 |