Toxicological analysis and efficacy of 2-phenylchromone on mycobacteria viability and inflammatory response induced by Mycobacterium bovis

• Autor(a) principal: Moreira, Flora Martinez Figueira
• Data de Publicação: 2021
• Outros Autores: Radai, Joyce Alencar Santos, de Souza, Vanessa Vilamaior, Berno, Claudia Rodrigues, de Araújo, Flavio Henrique Souza, Andrade-Silva, Magaiver, Oliveira, Rodrigo Juliano, Arena, Arielle Cristina [UNESP], Henriques, Maria das Graças Müller de Oliveira, Kassuya, Candida Aparecida Leite, Croda, Julio
• Tipo de documento: Artigo
• Idioma: eng
• Título da fonte: Repositório Institucional da UNESP
• Texto Completo: http://dx.doi.org/10.1016/j.phyplu.2021.100117; http://hdl.handle.net/11449/234218
• topic: Acute toxicity; Comet assay; Micronucleus test; Minimum inhibitory concentration; Mycobacterium tuberculosis; Pleurisy

Background: Studies have reported that compounds similar to flavone present antimicrobial and anti-inflammatory properties; however these effects are poorly described in the context of mycobacterium infection. Purpose: This study investigated the efficacy of 2-phenylchromone (Flavone) on mycobacteria viability and inflammatory response induced by Mycobacterium bovis, as well as its toxicological potential in in vitro and in vivo models. Methods: In vitro antimycobacterial activity was performed using the resazurin microtiter assay method. In order to investigate the anti-inflammatory action, C57Bl/6 mice were pretreated orally with 2-phenylchromone (1–100 mg/kg) 1 h before the pleurisy induction. The leukocyte migration and cytokine production were evaluated. Acute oral toxicity test, genotoxicity evaluations and splenic phagocytosis assay were also evaluated. Results: The minimum inhibitory concentration of 2-phenylchromone in the presence of the Mycobacterium tuberculosis strain was 28.90 µg/mL. In the BCG-induced pleurisy model, the oral treatment with 2-phenylchromone (1, 10, or 100 mg/kg) caused a significant reduction in the tumor necrosis factor (TNF) levels in the pleural exudate, as well as in the total and differential leukocyte counts. The oral treatment with 2-phenylchromone also did not present acute toxicity or genotoxicity in comet assay and micronucleus tests. However, 2-phenylchromone induced an increase of 1.48-times in splenic phagocytosis in the animals. Conclusion: Our results showed that 2-phenylchromone did not induced toxicity signs and is effective against Mycobacterium viability and reduced the inflammatory response elicited by mycobacteria.