Characterization and transferability of microsatellite markers of the cultivated peanut (Arachis hypogaea)
Autor(a) principal: | |
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Data de Publicação: | 2007 |
Outros Autores: | , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UNESP |
Texto Completo: | http://dx.doi.org/10.1186/1471-2229-7-9 http://hdl.handle.net/11449/17857 |
Resumo: | Background: the genus Arachis includes Arachis hypogaea (cultivated peanut) and wild species that are used in peanut breeding or as forage. Molecular markers have been employed in several studies of this genus, but microsatellite markers have only been used in few investigations. Microsatellites are very informative and are useful to assess genetic variability, analyze mating systems and in genetic mapping. The objectives of this study were to develop A. hypogaea microsatellite loci and to evaluate the transferability of these markers to other Arachis species.Results: Thirteen loci were isolated and characterized using 16 accessions of A. hypogaea. The level of variation found in A. hypogaea using microsatellites was higher than with other markers. Cross-transferability of the markers was also high. Sequencing of the fragments amplified using the primer pair AhII from 17 wild Arachis species showed that almost all wild species had similar repeated sequence to the one observed in A. hypogaea. Sequence data suggested that there is no correlation between taxonomic relationship of a wild species to A. hypogaea and the number of repeats found in its microsatellite loci.Conclusion: These results show that microsatellite primer pairs from A. hypogaea have multiple uses. A higher level of variation among A. hypogaea accessions can be detected using microsatellite markers in comparison to other markers, such as RFLP, RAPD and AFLP. The microsatellite primers of A. hypogaea showed a very high rate of transferability to other species of the genus. These primer pairs provide important tools to evaluate the genetic variability and to assess the mating system in Arachis species. |
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Repositório Institucional da UNESP |
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Characterization and transferability of microsatellite markers of the cultivated peanut (Arachis hypogaea)Background: the genus Arachis includes Arachis hypogaea (cultivated peanut) and wild species that are used in peanut breeding or as forage. Molecular markers have been employed in several studies of this genus, but microsatellite markers have only been used in few investigations. Microsatellites are very informative and are useful to assess genetic variability, analyze mating systems and in genetic mapping. The objectives of this study were to develop A. hypogaea microsatellite loci and to evaluate the transferability of these markers to other Arachis species.Results: Thirteen loci were isolated and characterized using 16 accessions of A. hypogaea. The level of variation found in A. hypogaea using microsatellites was higher than with other markers. Cross-transferability of the markers was also high. Sequencing of the fragments amplified using the primer pair AhII from 17 wild Arachis species showed that almost all wild species had similar repeated sequence to the one observed in A. hypogaea. Sequence data suggested that there is no correlation between taxonomic relationship of a wild species to A. hypogaea and the number of repeats found in its microsatellite loci.Conclusion: These results show that microsatellite primer pairs from A. hypogaea have multiple uses. A higher level of variation among A. hypogaea accessions can be detected using microsatellite markers in comparison to other markers, such as RFLP, RAPD and AFLP. The microsatellite primers of A. hypogaea showed a very high rate of transferability to other species of the genus. These primer pairs provide important tools to evaluate the genetic variability and to assess the mating system in Arachis species.Univ Estadual Paulista, Inst Ciências, Dept Genet, Lab Biotecnol & Genet Mol,BIOGEM, Botucatu, SP, BrazilInst Agron Campinas, Campinas, SP, BrazilUniv Estadual Paulista, Inst Ciências, Dept Genet, Lab Biotecnol & Genet Mol,BIOGEM, Botucatu, SP, BrazilBiomed Central Ltd.Universidade Estadual Paulista (Unesp)Inst Agron CampinasGimenes, Marcos A.Hoshino, Andrea A.Barbosa, Andrea V. G.Palmieri, Dario A.Lopes, Catalina R.2014-05-20T13:50:04Z2014-05-20T13:50:04Z2007-02-27info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article13application/pdfhttp://dx.doi.org/10.1186/1471-2229-7-9Bmc Plant Biology. London: Biomed Central Ltd., v. 7, 13 p., 2007.1471-2229http://hdl.handle.net/11449/1785710.1186/1471-2229-7-9WOS:000245255600001WOS000245255600001.pdfWeb of Sciencereponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengBmc Plant Biology3.9301,887info:eu-repo/semantics/openAccess2023-10-12T06:02:55Zoai:repositorio.unesp.br:11449/17857Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462024-08-05T14:39:41.779422Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false |
dc.title.none.fl_str_mv |
Characterization and transferability of microsatellite markers of the cultivated peanut (Arachis hypogaea) |
title |
Characterization and transferability of microsatellite markers of the cultivated peanut (Arachis hypogaea) |
spellingShingle |
Characterization and transferability of microsatellite markers of the cultivated peanut (Arachis hypogaea) Gimenes, Marcos A. |
title_short |
Characterization and transferability of microsatellite markers of the cultivated peanut (Arachis hypogaea) |
title_full |
Characterization and transferability of microsatellite markers of the cultivated peanut (Arachis hypogaea) |
title_fullStr |
Characterization and transferability of microsatellite markers of the cultivated peanut (Arachis hypogaea) |
title_full_unstemmed |
Characterization and transferability of microsatellite markers of the cultivated peanut (Arachis hypogaea) |
title_sort |
Characterization and transferability of microsatellite markers of the cultivated peanut (Arachis hypogaea) |
author |
Gimenes, Marcos A. |
author_facet |
Gimenes, Marcos A. Hoshino, Andrea A. Barbosa, Andrea V. G. Palmieri, Dario A. Lopes, Catalina R. |
author_role |
author |
author2 |
Hoshino, Andrea A. Barbosa, Andrea V. G. Palmieri, Dario A. Lopes, Catalina R. |
author2_role |
author author author author |
dc.contributor.none.fl_str_mv |
Universidade Estadual Paulista (Unesp) Inst Agron Campinas |
dc.contributor.author.fl_str_mv |
Gimenes, Marcos A. Hoshino, Andrea A. Barbosa, Andrea V. G. Palmieri, Dario A. Lopes, Catalina R. |
description |
Background: the genus Arachis includes Arachis hypogaea (cultivated peanut) and wild species that are used in peanut breeding or as forage. Molecular markers have been employed in several studies of this genus, but microsatellite markers have only been used in few investigations. Microsatellites are very informative and are useful to assess genetic variability, analyze mating systems and in genetic mapping. The objectives of this study were to develop A. hypogaea microsatellite loci and to evaluate the transferability of these markers to other Arachis species.Results: Thirteen loci were isolated and characterized using 16 accessions of A. hypogaea. The level of variation found in A. hypogaea using microsatellites was higher than with other markers. Cross-transferability of the markers was also high. Sequencing of the fragments amplified using the primer pair AhII from 17 wild Arachis species showed that almost all wild species had similar repeated sequence to the one observed in A. hypogaea. Sequence data suggested that there is no correlation between taxonomic relationship of a wild species to A. hypogaea and the number of repeats found in its microsatellite loci.Conclusion: These results show that microsatellite primer pairs from A. hypogaea have multiple uses. A higher level of variation among A. hypogaea accessions can be detected using microsatellite markers in comparison to other markers, such as RFLP, RAPD and AFLP. The microsatellite primers of A. hypogaea showed a very high rate of transferability to other species of the genus. These primer pairs provide important tools to evaluate the genetic variability and to assess the mating system in Arachis species. |
publishDate |
2007 |
dc.date.none.fl_str_mv |
2007-02-27 2014-05-20T13:50:04Z 2014-05-20T13:50:04Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://dx.doi.org/10.1186/1471-2229-7-9 Bmc Plant Biology. London: Biomed Central Ltd., v. 7, 13 p., 2007. 1471-2229 http://hdl.handle.net/11449/17857 10.1186/1471-2229-7-9 WOS:000245255600001 WOS000245255600001.pdf |
url |
http://dx.doi.org/10.1186/1471-2229-7-9 http://hdl.handle.net/11449/17857 |
identifier_str_mv |
Bmc Plant Biology. London: Biomed Central Ltd., v. 7, 13 p., 2007. 1471-2229 10.1186/1471-2229-7-9 WOS:000245255600001 WOS000245255600001.pdf |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Bmc Plant Biology 3.930 1,887 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
13 application/pdf |
dc.publisher.none.fl_str_mv |
Biomed Central Ltd. |
publisher.none.fl_str_mv |
Biomed Central Ltd. |
dc.source.none.fl_str_mv |
Web of Science reponame:Repositório Institucional da UNESP instname:Universidade Estadual Paulista (UNESP) instacron:UNESP |
instname_str |
Universidade Estadual Paulista (UNESP) |
instacron_str |
UNESP |
institution |
UNESP |
reponame_str |
Repositório Institucional da UNESP |
collection |
Repositório Institucional da UNESP |
repository.name.fl_str_mv |
Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP) |
repository.mail.fl_str_mv |
|
_version_ |
1808128396168790016 |