Positive effects of antitumor drugs in combination with propolis on canine osteosarcoma cells (spOS-2) and mesenchymal stem cells
Autor(a) principal: | |
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Data de Publicação: | 2018 |
Outros Autores: | , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UNESP |
Texto Completo: | http://dx.doi.org/10.1016/j.biopha.2018.05.027 http://hdl.handle.net/11449/179862 |
Resumo: | The combination of lower concentrations of antitumor drugs (carboplatin – CARB, doxorubicin – DOX, and methotrexate – MET) with propolis was investigated against canine osteosarcoma (spOS-2) and mesenchymal stem cells (MSC) in vitro. The mechanism of action in the combinations was analyzed. spOS-2 cells were incubated up to 72 h with propolis (50 μg/ml) alone or in combination with CARB (10–400 μmol/l), DOX (0.5–2 μmol/l) or MET (50–200 μmol/l). Cell viability was assessed by MTT assay, apoptosis/necrosis by flow cytometry, and MSC was incubated with the optimum combination. Propolis alone exerted no cytotoxic action against spOS-2 cells, whereas CARB (400, 200 and 100 μmol/l) exhibited the highest cytotoxic effects comparing to DOX and MET. The combination of propolis with the lowest concentrations of CARB led to better results comparing to CARB alone, which was not observed using DOX and MET. Apoptosis was involved in the action of propolis + CARB in spOS-2 cells. MSC were not affected by CARB/propolis, indicating that the cytotoxic action of the combination was specific to tumor cells but not to normal ones. Propolis improved the action of CARB against spOS-2 cells using lower concentrations of this drug, without affecting MSC. These findings are relevant and indicate a possible application of propolis in OSA treatment. |
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Positive effects of antitumor drugs in combination with propolis on canine osteosarcoma cells (spOS-2) and mesenchymal stem cellsApoptosisCytotoxic actionOsteosarcomaPropolisThe combination of lower concentrations of antitumor drugs (carboplatin – CARB, doxorubicin – DOX, and methotrexate – MET) with propolis was investigated against canine osteosarcoma (spOS-2) and mesenchymal stem cells (MSC) in vitro. The mechanism of action in the combinations was analyzed. spOS-2 cells were incubated up to 72 h with propolis (50 μg/ml) alone or in combination with CARB (10–400 μmol/l), DOX (0.5–2 μmol/l) or MET (50–200 μmol/l). Cell viability was assessed by MTT assay, apoptosis/necrosis by flow cytometry, and MSC was incubated with the optimum combination. Propolis alone exerted no cytotoxic action against spOS-2 cells, whereas CARB (400, 200 and 100 μmol/l) exhibited the highest cytotoxic effects comparing to DOX and MET. The combination of propolis with the lowest concentrations of CARB led to better results comparing to CARB alone, which was not observed using DOX and MET. Apoptosis was involved in the action of propolis + CARB in spOS-2 cells. MSC were not affected by CARB/propolis, indicating that the cytotoxic action of the combination was specific to tumor cells but not to normal ones. Propolis improved the action of CARB against spOS-2 cells using lower concentrations of this drug, without affecting MSC. These findings are relevant and indicate a possible application of propolis in OSA treatment.Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)São Paulo State University (UNESP) Institute of Biosciences, Campus BotucatuLaboratory of Pathology and Legal Veterinary Medicine Faculty of Veterinary Medicine State University of CearáPharmaceutical-Biochemistry Technology Department School of Pharmaceutical Science University of São PauloSão Paulo State University (UNESP) Institute of Biosciences, Campus BotucatuFAPESP: 2009/53493-9FAPESP: 2012/21158-9Universidade Estadual Paulista (Unesp)State University of CearáUniversidade de São Paulo (USP)Bernardino, Pedro Negri [UNESP]Bersano, Paulo Ricardo OliveiraLima Neto, João FerreiraSforcin, José Maurício [UNESP]2018-12-11T17:37:04Z2018-12-11T17:37:04Z2018-08-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article268-274application/pdfhttp://dx.doi.org/10.1016/j.biopha.2018.05.027Biomedicine and Pharmacotherapy, v. 104, p. 268-274.1950-60070753-3322http://hdl.handle.net/11449/17986210.1016/j.biopha.2018.05.0272-s2.0-850470073632-s2.0-85047007363.pdfScopusreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengBiomedicine and Pharmacotherapy0,951info:eu-repo/semantics/openAccess2023-11-20T06:14:47Zoai:repositorio.unesp.br:11449/179862Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462024-08-05T18:15:46.606791Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false |
dc.title.none.fl_str_mv |
Positive effects of antitumor drugs in combination with propolis on canine osteosarcoma cells (spOS-2) and mesenchymal stem cells |
title |
Positive effects of antitumor drugs in combination with propolis on canine osteosarcoma cells (spOS-2) and mesenchymal stem cells |
spellingShingle |
Positive effects of antitumor drugs in combination with propolis on canine osteosarcoma cells (spOS-2) and mesenchymal stem cells Bernardino, Pedro Negri [UNESP] Apoptosis Cytotoxic action Osteosarcoma Propolis |
title_short |
Positive effects of antitumor drugs in combination with propolis on canine osteosarcoma cells (spOS-2) and mesenchymal stem cells |
title_full |
Positive effects of antitumor drugs in combination with propolis on canine osteosarcoma cells (spOS-2) and mesenchymal stem cells |
title_fullStr |
Positive effects of antitumor drugs in combination with propolis on canine osteosarcoma cells (spOS-2) and mesenchymal stem cells |
title_full_unstemmed |
Positive effects of antitumor drugs in combination with propolis on canine osteosarcoma cells (spOS-2) and mesenchymal stem cells |
title_sort |
Positive effects of antitumor drugs in combination with propolis on canine osteosarcoma cells (spOS-2) and mesenchymal stem cells |
author |
Bernardino, Pedro Negri [UNESP] |
author_facet |
Bernardino, Pedro Negri [UNESP] Bersano, Paulo Ricardo Oliveira Lima Neto, João Ferreira Sforcin, José Maurício [UNESP] |
author_role |
author |
author2 |
Bersano, Paulo Ricardo Oliveira Lima Neto, João Ferreira Sforcin, José Maurício [UNESP] |
author2_role |
author author author |
dc.contributor.none.fl_str_mv |
Universidade Estadual Paulista (Unesp) State University of Ceará Universidade de São Paulo (USP) |
dc.contributor.author.fl_str_mv |
Bernardino, Pedro Negri [UNESP] Bersano, Paulo Ricardo Oliveira Lima Neto, João Ferreira Sforcin, José Maurício [UNESP] |
dc.subject.por.fl_str_mv |
Apoptosis Cytotoxic action Osteosarcoma Propolis |
topic |
Apoptosis Cytotoxic action Osteosarcoma Propolis |
description |
The combination of lower concentrations of antitumor drugs (carboplatin – CARB, doxorubicin – DOX, and methotrexate – MET) with propolis was investigated against canine osteosarcoma (spOS-2) and mesenchymal stem cells (MSC) in vitro. The mechanism of action in the combinations was analyzed. spOS-2 cells were incubated up to 72 h with propolis (50 μg/ml) alone or in combination with CARB (10–400 μmol/l), DOX (0.5–2 μmol/l) or MET (50–200 μmol/l). Cell viability was assessed by MTT assay, apoptosis/necrosis by flow cytometry, and MSC was incubated with the optimum combination. Propolis alone exerted no cytotoxic action against spOS-2 cells, whereas CARB (400, 200 and 100 μmol/l) exhibited the highest cytotoxic effects comparing to DOX and MET. The combination of propolis with the lowest concentrations of CARB led to better results comparing to CARB alone, which was not observed using DOX and MET. Apoptosis was involved in the action of propolis + CARB in spOS-2 cells. MSC were not affected by CARB/propolis, indicating that the cytotoxic action of the combination was specific to tumor cells but not to normal ones. Propolis improved the action of CARB against spOS-2 cells using lower concentrations of this drug, without affecting MSC. These findings are relevant and indicate a possible application of propolis in OSA treatment. |
publishDate |
2018 |
dc.date.none.fl_str_mv |
2018-12-11T17:37:04Z 2018-12-11T17:37:04Z 2018-08-01 |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://dx.doi.org/10.1016/j.biopha.2018.05.027 Biomedicine and Pharmacotherapy, v. 104, p. 268-274. 1950-6007 0753-3322 http://hdl.handle.net/11449/179862 10.1016/j.biopha.2018.05.027 2-s2.0-85047007363 2-s2.0-85047007363.pdf |
url |
http://dx.doi.org/10.1016/j.biopha.2018.05.027 http://hdl.handle.net/11449/179862 |
identifier_str_mv |
Biomedicine and Pharmacotherapy, v. 104, p. 268-274. 1950-6007 0753-3322 10.1016/j.biopha.2018.05.027 2-s2.0-85047007363 2-s2.0-85047007363.pdf |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Biomedicine and Pharmacotherapy 0,951 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
268-274 application/pdf |
dc.source.none.fl_str_mv |
Scopus reponame:Repositório Institucional da UNESP instname:Universidade Estadual Paulista (UNESP) instacron:UNESP |
instname_str |
Universidade Estadual Paulista (UNESP) |
instacron_str |
UNESP |
institution |
UNESP |
reponame_str |
Repositório Institucional da UNESP |
collection |
Repositório Institucional da UNESP |
repository.name.fl_str_mv |
Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP) |
repository.mail.fl_str_mv |
|
_version_ |
1808128912615538688 |