Cell-mediated immune response to Leishmania chagasi experimental infection of BALB/c immunosuppressed mice
Autor(a) principal: | |
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Data de Publicação: | 2010 |
Outros Autores: | , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UNESP |
Texto Completo: | http://dx.doi.org/10.1590/S1678-91992010000100013 http://hdl.handle.net/11449/225828 |
Resumo: | Leishmaniasis, a zoonosis of worldwide distribution, presents a significant impact on immunosupressed patients. This study aimed to evaluate Leishmania chagasi infection in BALB/c mice immunosuppressed with dexamethasone. Spleen cells stimulated or not with L. chagasi were cultured for cytokine quantification (IFN-γ, IL-2, IL-4 and IL-10) by sandwich ELISA. Parasite loads in the spleen and liver were determined by means of culture microtitration. Immunosuppressed groups showed statistically lower spleen weight and CD4-cell percentage in blood on the day of infection and produced Th1 and Th2 cytokines on other days of the study. The other infected groups, weather immunosupressed or not, also produced Th1 and Th2 cytokines. Parasite loads in the spleen and liver were not statistically different among the groups. It was concluded that L. chagasi infection was not affected by dexamethasone-induced immunosuppression, probably due the reversible effect of the treatment. |
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Cell-mediated immune response to Leishmania chagasi experimental infection of BALB/c immunosuppressed miceImmunopathologyImmunosuppressionInterleukinsMiceVisceral leishmaniasisLeishmaniasis, a zoonosis of worldwide distribution, presents a significant impact on immunosupressed patients. This study aimed to evaluate Leishmania chagasi infection in BALB/c mice immunosuppressed with dexamethasone. Spleen cells stimulated or not with L. chagasi were cultured for cytokine quantification (IFN-γ, IL-2, IL-4 and IL-10) by sandwich ELISA. Parasite loads in the spleen and liver were determined by means of culture microtitration. Immunosuppressed groups showed statistically lower spleen weight and CD4-cell percentage in blood on the day of infection and produced Th1 and Th2 cytokines on other days of the study. The other infected groups, weather immunosupressed or not, also produced Th1 and Th2 cytokines. Parasite loads in the spleen and liver were not statistically different among the groups. It was concluded that L. chagasi infection was not affected by dexamethasone-induced immunosuppression, probably due the reversible effect of the treatment.Zoonosis Research Center NUPEZO Veterinary Medicine and Animal Husbandry School São Paulo State University UNESP, Botucatu, São Paulo StateDepartment of Microbiology and Immunology Botucatu Biosciences Institute São Paulo State University UNESP, Botucatu, São Paulo StateZoonosis Research Center NUPEZO Veterinary Medicine and Animal Husbandry School São Paulo State University UNESP, Botucatu, São Paulo StateDepartment of Microbiology and Immunology Botucatu Biosciences Institute São Paulo State University UNESP, Botucatu, São Paulo StateUniversidade Estadual Paulista (UNESP)Machado, J. G. [UNESP]Hoffmann, J. L. [UNESP]Krause, V. L.K. [UNESP]da Silva, A. V. [UNESP]Dias-Melicio, L. A. [UNESP]Langoni, H. [UNESP]2022-04-28T21:00:55Z2022-04-28T21:00:55Z2010-01-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article131-146http://dx.doi.org/10.1590/S1678-91992010000100013Journal of Venomous Animals and Toxins Including Tropical Diseases, v. 16, n. 1, p. 131-146, 2010.1678-9199http://hdl.handle.net/11449/22582810.1590/S1678-919920100001000132-s2.0-77950854148Scopusreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengJournal of Venomous Animals and Toxins Including Tropical Diseasesinfo:eu-repo/semantics/openAccess2022-04-28T21:00:55Zoai:repositorio.unesp.br:11449/225828Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462024-08-05T21:00:30.441897Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false |
dc.title.none.fl_str_mv |
Cell-mediated immune response to Leishmania chagasi experimental infection of BALB/c immunosuppressed mice |
title |
Cell-mediated immune response to Leishmania chagasi experimental infection of BALB/c immunosuppressed mice |
spellingShingle |
Cell-mediated immune response to Leishmania chagasi experimental infection of BALB/c immunosuppressed mice Machado, J. G. [UNESP] Immunopathology Immunosuppression Interleukins Mice Visceral leishmaniasis |
title_short |
Cell-mediated immune response to Leishmania chagasi experimental infection of BALB/c immunosuppressed mice |
title_full |
Cell-mediated immune response to Leishmania chagasi experimental infection of BALB/c immunosuppressed mice |
title_fullStr |
Cell-mediated immune response to Leishmania chagasi experimental infection of BALB/c immunosuppressed mice |
title_full_unstemmed |
Cell-mediated immune response to Leishmania chagasi experimental infection of BALB/c immunosuppressed mice |
title_sort |
Cell-mediated immune response to Leishmania chagasi experimental infection of BALB/c immunosuppressed mice |
author |
Machado, J. G. [UNESP] |
author_facet |
Machado, J. G. [UNESP] Hoffmann, J. L. [UNESP] Krause, V. L.K. [UNESP] da Silva, A. V. [UNESP] Dias-Melicio, L. A. [UNESP] Langoni, H. [UNESP] |
author_role |
author |
author2 |
Hoffmann, J. L. [UNESP] Krause, V. L.K. [UNESP] da Silva, A. V. [UNESP] Dias-Melicio, L. A. [UNESP] Langoni, H. [UNESP] |
author2_role |
author author author author author |
dc.contributor.none.fl_str_mv |
Universidade Estadual Paulista (UNESP) |
dc.contributor.author.fl_str_mv |
Machado, J. G. [UNESP] Hoffmann, J. L. [UNESP] Krause, V. L.K. [UNESP] da Silva, A. V. [UNESP] Dias-Melicio, L. A. [UNESP] Langoni, H. [UNESP] |
dc.subject.por.fl_str_mv |
Immunopathology Immunosuppression Interleukins Mice Visceral leishmaniasis |
topic |
Immunopathology Immunosuppression Interleukins Mice Visceral leishmaniasis |
description |
Leishmaniasis, a zoonosis of worldwide distribution, presents a significant impact on immunosupressed patients. This study aimed to evaluate Leishmania chagasi infection in BALB/c mice immunosuppressed with dexamethasone. Spleen cells stimulated or not with L. chagasi were cultured for cytokine quantification (IFN-γ, IL-2, IL-4 and IL-10) by sandwich ELISA. Parasite loads in the spleen and liver were determined by means of culture microtitration. Immunosuppressed groups showed statistically lower spleen weight and CD4-cell percentage in blood on the day of infection and produced Th1 and Th2 cytokines on other days of the study. The other infected groups, weather immunosupressed or not, also produced Th1 and Th2 cytokines. Parasite loads in the spleen and liver were not statistically different among the groups. It was concluded that L. chagasi infection was not affected by dexamethasone-induced immunosuppression, probably due the reversible effect of the treatment. |
publishDate |
2010 |
dc.date.none.fl_str_mv |
2010-01-01 2022-04-28T21:00:55Z 2022-04-28T21:00:55Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://dx.doi.org/10.1590/S1678-91992010000100013 Journal of Venomous Animals and Toxins Including Tropical Diseases, v. 16, n. 1, p. 131-146, 2010. 1678-9199 http://hdl.handle.net/11449/225828 10.1590/S1678-91992010000100013 2-s2.0-77950854148 |
url |
http://dx.doi.org/10.1590/S1678-91992010000100013 http://hdl.handle.net/11449/225828 |
identifier_str_mv |
Journal of Venomous Animals and Toxins Including Tropical Diseases, v. 16, n. 1, p. 131-146, 2010. 1678-9199 10.1590/S1678-91992010000100013 2-s2.0-77950854148 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Journal of Venomous Animals and Toxins Including Tropical Diseases |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
131-146 |
dc.source.none.fl_str_mv |
Scopus reponame:Repositório Institucional da UNESP instname:Universidade Estadual Paulista (UNESP) instacron:UNESP |
instname_str |
Universidade Estadual Paulista (UNESP) |
instacron_str |
UNESP |
institution |
UNESP |
reponame_str |
Repositório Institucional da UNESP |
collection |
Repositório Institucional da UNESP |
repository.name.fl_str_mv |
Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP) |
repository.mail.fl_str_mv |
|
_version_ |
1808129272819220480 |