Analgesic and anti-inflammatory articular effects of essential oil and camphor isolated from Ocimum kilimandscharicum Gürke leaves
Autor(a) principal: | |
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Data de Publicação: | 2021 |
Outros Autores: | , , , , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UNESP |
Texto Completo: | http://dx.doi.org/10.1016/j.jep.2020.113697 http://hdl.handle.net/11449/208289 |
Resumo: | Ethnopharmacological relevance: Leaves from Ocimum kilimandscharicum Gürke (Lamiaceae) are popularly used against articular pain. Aim of study: The aim of this study was to test the anti-inflammatory and anti-hyperalgesic (analgesic) properties of the essential oil and camphor isolated from O. Kilimandscharicum leaves (EOOK) in 4 models including zymosan induced-articular inflammation model in mice. Material and methods: For in vivo models, EOOK was tested in carrageenan-induced paw edema model with oral doses of 30, 100, and 300 mg/kg (oral administration = p.o.) and in zymosan-induced articular inflammation (including knee edema, leukocyte infiltration, mechanical hyperalgesia and nitric oxide), EOOK (100 mg/kg, p. o.) and camphor (30 mg/kg, p. o.) were tested. EOOK (100 mg/kg, p. o.) was tested in the rolling and also in the adhesion of leukocytes to the mesenteric microcirculation in situ model of carrageenan induced inflammation and EOOK (1, 3, 10, 30, and 60 μg/mL) was tested in vitro against neutrophils chemotaxis induced by N-formyl methionyl leucyl phenylalanine (fMLP). Results: The treatment with EOOK significantly inhibited the carrageenan-induced edema, mechanical and cold hyperalgesia. Both, EOOK and camphor inhibited all articular parameters induced by zymosan. In situ intravitral microscopy analysis, EOOK significantly inhibited carrageenan-induced leukocyte rolling and adhesion. In vitro neutrophils chemotaxis, EOOK inhibited the leukocyte chemotaxis induced by fMLP. Conclusions: The present study showed that EOOK inhibited pain and inflammatory parameters contributing, at least in part, to explain the popular use of this plant as analgesic natural agent. This study also demonstrates that camphor and some known anti-inflammatory compounds present in EOOK could contribute for analgesic and anti-inflammatory articular properties. |
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Analgesic and anti-inflammatory articular effects of essential oil and camphor isolated from Ocimum kilimandscharicum Gürke leavesAnti-hyperalgesicArticularCamphorInflammationJointOcimum kilimandscharicumEthnopharmacological relevance: Leaves from Ocimum kilimandscharicum Gürke (Lamiaceae) are popularly used against articular pain. Aim of study: The aim of this study was to test the anti-inflammatory and anti-hyperalgesic (analgesic) properties of the essential oil and camphor isolated from O. Kilimandscharicum leaves (EOOK) in 4 models including zymosan induced-articular inflammation model in mice. Material and methods: For in vivo models, EOOK was tested in carrageenan-induced paw edema model with oral doses of 30, 100, and 300 mg/kg (oral administration = p.o.) and in zymosan-induced articular inflammation (including knee edema, leukocyte infiltration, mechanical hyperalgesia and nitric oxide), EOOK (100 mg/kg, p. o.) and camphor (30 mg/kg, p. o.) were tested. EOOK (100 mg/kg, p. o.) was tested in the rolling and also in the adhesion of leukocytes to the mesenteric microcirculation in situ model of carrageenan induced inflammation and EOOK (1, 3, 10, 30, and 60 μg/mL) was tested in vitro against neutrophils chemotaxis induced by N-formyl methionyl leucyl phenylalanine (fMLP). Results: The treatment with EOOK significantly inhibited the carrageenan-induced edema, mechanical and cold hyperalgesia. Both, EOOK and camphor inhibited all articular parameters induced by zymosan. In situ intravitral microscopy analysis, EOOK significantly inhibited carrageenan-induced leukocyte rolling and adhesion. In vitro neutrophils chemotaxis, EOOK inhibited the leukocyte chemotaxis induced by fMLP. Conclusions: The present study showed that EOOK inhibited pain and inflammatory parameters contributing, at least in part, to explain the popular use of this plant as analgesic natural agent. This study also demonstrates that camphor and some known anti-inflammatory compounds present in EOOK could contribute for analgesic and anti-inflammatory articular properties.Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)Fundação de Apoio ao Desenvolvimento do Ensino, Ciência e Tecnologia do Estado de Mato Grosso do SulSchool of Health Sciences Federal University of Grande DouradosSchool of Health Sciences University Center of Grande Dourados (UNIGRAN)Pharmaceutical Sciences Food and Nutrition College Federal University of Mato Grosso do SulDepartment of Structural and Functional Biology Institute of Biosciences of Botucatu Universidade Estadual Paulista (UNESP)Department of Pharmacology and Therapeutics State University of Maringá (UEM)School of Medicine Federal University of Mato Grosso do SulDepartment of Structural and Functional Biology Institute of Biosciences of Botucatu Universidade Estadual Paulista (UNESP)Fundação de Apoio ao Desenvolvimento do Ensino, Ciência e Tecnologia do Estado de Mato Grosso do Sul: 59/300.130/2016Federal University of Grande DouradosUniversity Center of Grande Dourados (UNIGRAN)Federal University of Mato Grosso do SulUniversidade Estadual Paulista (Unesp)Universidade Estadual de Maringá (UEM)dos Santos, ElisangelaLeitão, Maicon MatosAguero Ito, Caren NaomiSilva-Filho, Saulo EuclidesArena, Arielle Cristina [UNESP]Silva-Comar, Francielli Maria de SouzaNakamura Cuman, Roberto KenjiOliveira, Rodrigo JulianoNazari Formagio, Anelise SamaraLeite Kassuya, Cândida Aparecida2021-06-25T11:09:46Z2021-06-25T11:09:46Z2021-04-06info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articlehttp://dx.doi.org/10.1016/j.jep.2020.113697Journal of Ethnopharmacology, v. 269.1872-75730378-8741http://hdl.handle.net/11449/20828910.1016/j.jep.2020.1136972-s2.0-85098865873Scopusreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengJournal of Ethnopharmacologyinfo:eu-repo/semantics/openAccess2021-10-23T18:56:56Zoai:repositorio.unesp.br:11449/208289Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462024-08-05T15:09:55.301924Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false |
dc.title.none.fl_str_mv |
Analgesic and anti-inflammatory articular effects of essential oil and camphor isolated from Ocimum kilimandscharicum Gürke leaves |
title |
Analgesic and anti-inflammatory articular effects of essential oil and camphor isolated from Ocimum kilimandscharicum Gürke leaves |
spellingShingle |
Analgesic and anti-inflammatory articular effects of essential oil and camphor isolated from Ocimum kilimandscharicum Gürke leaves dos Santos, Elisangela Anti-hyperalgesic Articular Camphor Inflammation Joint Ocimum kilimandscharicum |
title_short |
Analgesic and anti-inflammatory articular effects of essential oil and camphor isolated from Ocimum kilimandscharicum Gürke leaves |
title_full |
Analgesic and anti-inflammatory articular effects of essential oil and camphor isolated from Ocimum kilimandscharicum Gürke leaves |
title_fullStr |
Analgesic and anti-inflammatory articular effects of essential oil and camphor isolated from Ocimum kilimandscharicum Gürke leaves |
title_full_unstemmed |
Analgesic and anti-inflammatory articular effects of essential oil and camphor isolated from Ocimum kilimandscharicum Gürke leaves |
title_sort |
Analgesic and anti-inflammatory articular effects of essential oil and camphor isolated from Ocimum kilimandscharicum Gürke leaves |
author |
dos Santos, Elisangela |
author_facet |
dos Santos, Elisangela Leitão, Maicon Matos Aguero Ito, Caren Naomi Silva-Filho, Saulo Euclides Arena, Arielle Cristina [UNESP] Silva-Comar, Francielli Maria de Souza Nakamura Cuman, Roberto Kenji Oliveira, Rodrigo Juliano Nazari Formagio, Anelise Samara Leite Kassuya, Cândida Aparecida |
author_role |
author |
author2 |
Leitão, Maicon Matos Aguero Ito, Caren Naomi Silva-Filho, Saulo Euclides Arena, Arielle Cristina [UNESP] Silva-Comar, Francielli Maria de Souza Nakamura Cuman, Roberto Kenji Oliveira, Rodrigo Juliano Nazari Formagio, Anelise Samara Leite Kassuya, Cândida Aparecida |
author2_role |
author author author author author author author author author |
dc.contributor.none.fl_str_mv |
Federal University of Grande Dourados University Center of Grande Dourados (UNIGRAN) Federal University of Mato Grosso do Sul Universidade Estadual Paulista (Unesp) Universidade Estadual de Maringá (UEM) |
dc.contributor.author.fl_str_mv |
dos Santos, Elisangela Leitão, Maicon Matos Aguero Ito, Caren Naomi Silva-Filho, Saulo Euclides Arena, Arielle Cristina [UNESP] Silva-Comar, Francielli Maria de Souza Nakamura Cuman, Roberto Kenji Oliveira, Rodrigo Juliano Nazari Formagio, Anelise Samara Leite Kassuya, Cândida Aparecida |
dc.subject.por.fl_str_mv |
Anti-hyperalgesic Articular Camphor Inflammation Joint Ocimum kilimandscharicum |
topic |
Anti-hyperalgesic Articular Camphor Inflammation Joint Ocimum kilimandscharicum |
description |
Ethnopharmacological relevance: Leaves from Ocimum kilimandscharicum Gürke (Lamiaceae) are popularly used against articular pain. Aim of study: The aim of this study was to test the anti-inflammatory and anti-hyperalgesic (analgesic) properties of the essential oil and camphor isolated from O. Kilimandscharicum leaves (EOOK) in 4 models including zymosan induced-articular inflammation model in mice. Material and methods: For in vivo models, EOOK was tested in carrageenan-induced paw edema model with oral doses of 30, 100, and 300 mg/kg (oral administration = p.o.) and in zymosan-induced articular inflammation (including knee edema, leukocyte infiltration, mechanical hyperalgesia and nitric oxide), EOOK (100 mg/kg, p. o.) and camphor (30 mg/kg, p. o.) were tested. EOOK (100 mg/kg, p. o.) was tested in the rolling and also in the adhesion of leukocytes to the mesenteric microcirculation in situ model of carrageenan induced inflammation and EOOK (1, 3, 10, 30, and 60 μg/mL) was tested in vitro against neutrophils chemotaxis induced by N-formyl methionyl leucyl phenylalanine (fMLP). Results: The treatment with EOOK significantly inhibited the carrageenan-induced edema, mechanical and cold hyperalgesia. Both, EOOK and camphor inhibited all articular parameters induced by zymosan. In situ intravitral microscopy analysis, EOOK significantly inhibited carrageenan-induced leukocyte rolling and adhesion. In vitro neutrophils chemotaxis, EOOK inhibited the leukocyte chemotaxis induced by fMLP. Conclusions: The present study showed that EOOK inhibited pain and inflammatory parameters contributing, at least in part, to explain the popular use of this plant as analgesic natural agent. This study also demonstrates that camphor and some known anti-inflammatory compounds present in EOOK could contribute for analgesic and anti-inflammatory articular properties. |
publishDate |
2021 |
dc.date.none.fl_str_mv |
2021-06-25T11:09:46Z 2021-06-25T11:09:46Z 2021-04-06 |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://dx.doi.org/10.1016/j.jep.2020.113697 Journal of Ethnopharmacology, v. 269. 1872-7573 0378-8741 http://hdl.handle.net/11449/208289 10.1016/j.jep.2020.113697 2-s2.0-85098865873 |
url |
http://dx.doi.org/10.1016/j.jep.2020.113697 http://hdl.handle.net/11449/208289 |
identifier_str_mv |
Journal of Ethnopharmacology, v. 269. 1872-7573 0378-8741 10.1016/j.jep.2020.113697 2-s2.0-85098865873 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Journal of Ethnopharmacology |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.source.none.fl_str_mv |
Scopus reponame:Repositório Institucional da UNESP instname:Universidade Estadual Paulista (UNESP) instacron:UNESP |
instname_str |
Universidade Estadual Paulista (UNESP) |
instacron_str |
UNESP |
institution |
UNESP |
reponame_str |
Repositório Institucional da UNESP |
collection |
Repositório Institucional da UNESP |
repository.name.fl_str_mv |
Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP) |
repository.mail.fl_str_mv |
|
_version_ |
1808128471431380992 |