Analgesic and anti-inflammatory articular effects of essential oil and camphor isolated from Ocimum kilimandscharicum Gürke leaves

Detalhes bibliográficos
Autor(a) principal: dos Santos, Elisangela
Data de Publicação: 2021
Outros Autores: Leitão, Maicon Matos, Aguero Ito, Caren Naomi, Silva-Filho, Saulo Euclides, Arena, Arielle Cristina [UNESP], Silva-Comar, Francielli Maria de Souza, Nakamura Cuman, Roberto Kenji, Oliveira, Rodrigo Juliano, Nazari Formagio, Anelise Samara, Leite Kassuya, Cândida Aparecida
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da UNESP
Texto Completo: http://dx.doi.org/10.1016/j.jep.2020.113697
http://hdl.handle.net/11449/208289
Resumo: Ethnopharmacological relevance: Leaves from Ocimum kilimandscharicum Gürke (Lamiaceae) are popularly used against articular pain. Aim of study: The aim of this study was to test the anti-inflammatory and anti-hyperalgesic (analgesic) properties of the essential oil and camphor isolated from O. Kilimandscharicum leaves (EOOK) in 4 models including zymosan induced-articular inflammation model in mice. Material and methods: For in vivo models, EOOK was tested in carrageenan-induced paw edema model with oral doses of 30, 100, and 300 mg/kg (oral administration = p.o.) and in zymosan-induced articular inflammation (including knee edema, leukocyte infiltration, mechanical hyperalgesia and nitric oxide), EOOK (100 mg/kg, p. o.) and camphor (30 mg/kg, p. o.) were tested. EOOK (100 mg/kg, p. o.) was tested in the rolling and also in the adhesion of leukocytes to the mesenteric microcirculation in situ model of carrageenan induced inflammation and EOOK (1, 3, 10, 30, and 60 μg/mL) was tested in vitro against neutrophils chemotaxis induced by N-formyl methionyl leucyl phenylalanine (fMLP). Results: The treatment with EOOK significantly inhibited the carrageenan-induced edema, mechanical and cold hyperalgesia. Both, EOOK and camphor inhibited all articular parameters induced by zymosan. In situ intravitral microscopy analysis, EOOK significantly inhibited carrageenan-induced leukocyte rolling and adhesion. In vitro neutrophils chemotaxis, EOOK inhibited the leukocyte chemotaxis induced by fMLP. Conclusions: The present study showed that EOOK inhibited pain and inflammatory parameters contributing, at least in part, to explain the popular use of this plant as analgesic natural agent. This study also demonstrates that camphor and some known anti-inflammatory compounds present in EOOK could contribute for analgesic and anti-inflammatory articular properties.
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spelling Analgesic and anti-inflammatory articular effects of essential oil and camphor isolated from Ocimum kilimandscharicum Gürke leavesAnti-hyperalgesicArticularCamphorInflammationJointOcimum kilimandscharicumEthnopharmacological relevance: Leaves from Ocimum kilimandscharicum Gürke (Lamiaceae) are popularly used against articular pain. Aim of study: The aim of this study was to test the anti-inflammatory and anti-hyperalgesic (analgesic) properties of the essential oil and camphor isolated from O. Kilimandscharicum leaves (EOOK) in 4 models including zymosan induced-articular inflammation model in mice. Material and methods: For in vivo models, EOOK was tested in carrageenan-induced paw edema model with oral doses of 30, 100, and 300 mg/kg (oral administration = p.o.) and in zymosan-induced articular inflammation (including knee edema, leukocyte infiltration, mechanical hyperalgesia and nitric oxide), EOOK (100 mg/kg, p. o.) and camphor (30 mg/kg, p. o.) were tested. EOOK (100 mg/kg, p. o.) was tested in the rolling and also in the adhesion of leukocytes to the mesenteric microcirculation in situ model of carrageenan induced inflammation and EOOK (1, 3, 10, 30, and 60 μg/mL) was tested in vitro against neutrophils chemotaxis induced by N-formyl methionyl leucyl phenylalanine (fMLP). Results: The treatment with EOOK significantly inhibited the carrageenan-induced edema, mechanical and cold hyperalgesia. Both, EOOK and camphor inhibited all articular parameters induced by zymosan. In situ intravitral microscopy analysis, EOOK significantly inhibited carrageenan-induced leukocyte rolling and adhesion. In vitro neutrophils chemotaxis, EOOK inhibited the leukocyte chemotaxis induced by fMLP. Conclusions: The present study showed that EOOK inhibited pain and inflammatory parameters contributing, at least in part, to explain the popular use of this plant as analgesic natural agent. This study also demonstrates that camphor and some known anti-inflammatory compounds present in EOOK could contribute for analgesic and anti-inflammatory articular properties.Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)Fundação de Apoio ao Desenvolvimento do Ensino, Ciência e Tecnologia do Estado de Mato Grosso do SulSchool of Health Sciences Federal University of Grande DouradosSchool of Health Sciences University Center of Grande Dourados (UNIGRAN)Pharmaceutical Sciences Food and Nutrition College Federal University of Mato Grosso do SulDepartment of Structural and Functional Biology Institute of Biosciences of Botucatu Universidade Estadual Paulista (UNESP)Department of Pharmacology and Therapeutics State University of Maringá (UEM)School of Medicine Federal University of Mato Grosso do SulDepartment of Structural and Functional Biology Institute of Biosciences of Botucatu Universidade Estadual Paulista (UNESP)Fundação de Apoio ao Desenvolvimento do Ensino, Ciência e Tecnologia do Estado de Mato Grosso do Sul: 59/300.130/2016Federal University of Grande DouradosUniversity Center of Grande Dourados (UNIGRAN)Federal University of Mato Grosso do SulUniversidade Estadual Paulista (Unesp)Universidade Estadual de Maringá (UEM)dos Santos, ElisangelaLeitão, Maicon MatosAguero Ito, Caren NaomiSilva-Filho, Saulo EuclidesArena, Arielle Cristina [UNESP]Silva-Comar, Francielli Maria de SouzaNakamura Cuman, Roberto KenjiOliveira, Rodrigo JulianoNazari Formagio, Anelise SamaraLeite Kassuya, Cândida Aparecida2021-06-25T11:09:46Z2021-06-25T11:09:46Z2021-04-06info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articlehttp://dx.doi.org/10.1016/j.jep.2020.113697Journal of Ethnopharmacology, v. 269.1872-75730378-8741http://hdl.handle.net/11449/20828910.1016/j.jep.2020.1136972-s2.0-85098865873Scopusreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengJournal of Ethnopharmacologyinfo:eu-repo/semantics/openAccess2021-10-23T18:56:56Zoai:repositorio.unesp.br:11449/208289Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462024-08-05T15:09:55.301924Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false
dc.title.none.fl_str_mv Analgesic and anti-inflammatory articular effects of essential oil and camphor isolated from Ocimum kilimandscharicum Gürke leaves
title Analgesic and anti-inflammatory articular effects of essential oil and camphor isolated from Ocimum kilimandscharicum Gürke leaves
spellingShingle Analgesic and anti-inflammatory articular effects of essential oil and camphor isolated from Ocimum kilimandscharicum Gürke leaves
dos Santos, Elisangela
Anti-hyperalgesic
Articular
Camphor
Inflammation
Joint
Ocimum kilimandscharicum
title_short Analgesic and anti-inflammatory articular effects of essential oil and camphor isolated from Ocimum kilimandscharicum Gürke leaves
title_full Analgesic and anti-inflammatory articular effects of essential oil and camphor isolated from Ocimum kilimandscharicum Gürke leaves
title_fullStr Analgesic and anti-inflammatory articular effects of essential oil and camphor isolated from Ocimum kilimandscharicum Gürke leaves
title_full_unstemmed Analgesic and anti-inflammatory articular effects of essential oil and camphor isolated from Ocimum kilimandscharicum Gürke leaves
title_sort Analgesic and anti-inflammatory articular effects of essential oil and camphor isolated from Ocimum kilimandscharicum Gürke leaves
author dos Santos, Elisangela
author_facet dos Santos, Elisangela
Leitão, Maicon Matos
Aguero Ito, Caren Naomi
Silva-Filho, Saulo Euclides
Arena, Arielle Cristina [UNESP]
Silva-Comar, Francielli Maria de Souza
Nakamura Cuman, Roberto Kenji
Oliveira, Rodrigo Juliano
Nazari Formagio, Anelise Samara
Leite Kassuya, Cândida Aparecida
author_role author
author2 Leitão, Maicon Matos
Aguero Ito, Caren Naomi
Silva-Filho, Saulo Euclides
Arena, Arielle Cristina [UNESP]
Silva-Comar, Francielli Maria de Souza
Nakamura Cuman, Roberto Kenji
Oliveira, Rodrigo Juliano
Nazari Formagio, Anelise Samara
Leite Kassuya, Cândida Aparecida
author2_role author
author
author
author
author
author
author
author
author
dc.contributor.none.fl_str_mv Federal University of Grande Dourados
University Center of Grande Dourados (UNIGRAN)
Federal University of Mato Grosso do Sul
Universidade Estadual Paulista (Unesp)
Universidade Estadual de Maringá (UEM)
dc.contributor.author.fl_str_mv dos Santos, Elisangela
Leitão, Maicon Matos
Aguero Ito, Caren Naomi
Silva-Filho, Saulo Euclides
Arena, Arielle Cristina [UNESP]
Silva-Comar, Francielli Maria de Souza
Nakamura Cuman, Roberto Kenji
Oliveira, Rodrigo Juliano
Nazari Formagio, Anelise Samara
Leite Kassuya, Cândida Aparecida
dc.subject.por.fl_str_mv Anti-hyperalgesic
Articular
Camphor
Inflammation
Joint
Ocimum kilimandscharicum
topic Anti-hyperalgesic
Articular
Camphor
Inflammation
Joint
Ocimum kilimandscharicum
description Ethnopharmacological relevance: Leaves from Ocimum kilimandscharicum Gürke (Lamiaceae) are popularly used against articular pain. Aim of study: The aim of this study was to test the anti-inflammatory and anti-hyperalgesic (analgesic) properties of the essential oil and camphor isolated from O. Kilimandscharicum leaves (EOOK) in 4 models including zymosan induced-articular inflammation model in mice. Material and methods: For in vivo models, EOOK was tested in carrageenan-induced paw edema model with oral doses of 30, 100, and 300 mg/kg (oral administration = p.o.) and in zymosan-induced articular inflammation (including knee edema, leukocyte infiltration, mechanical hyperalgesia and nitric oxide), EOOK (100 mg/kg, p. o.) and camphor (30 mg/kg, p. o.) were tested. EOOK (100 mg/kg, p. o.) was tested in the rolling and also in the adhesion of leukocytes to the mesenteric microcirculation in situ model of carrageenan induced inflammation and EOOK (1, 3, 10, 30, and 60 μg/mL) was tested in vitro against neutrophils chemotaxis induced by N-formyl methionyl leucyl phenylalanine (fMLP). Results: The treatment with EOOK significantly inhibited the carrageenan-induced edema, mechanical and cold hyperalgesia. Both, EOOK and camphor inhibited all articular parameters induced by zymosan. In situ intravitral microscopy analysis, EOOK significantly inhibited carrageenan-induced leukocyte rolling and adhesion. In vitro neutrophils chemotaxis, EOOK inhibited the leukocyte chemotaxis induced by fMLP. Conclusions: The present study showed that EOOK inhibited pain and inflammatory parameters contributing, at least in part, to explain the popular use of this plant as analgesic natural agent. This study also demonstrates that camphor and some known anti-inflammatory compounds present in EOOK could contribute for analgesic and anti-inflammatory articular properties.
publishDate 2021
dc.date.none.fl_str_mv 2021-06-25T11:09:46Z
2021-06-25T11:09:46Z
2021-04-06
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://dx.doi.org/10.1016/j.jep.2020.113697
Journal of Ethnopharmacology, v. 269.
1872-7573
0378-8741
http://hdl.handle.net/11449/208289
10.1016/j.jep.2020.113697
2-s2.0-85098865873
url http://dx.doi.org/10.1016/j.jep.2020.113697
http://hdl.handle.net/11449/208289
identifier_str_mv Journal of Ethnopharmacology, v. 269.
1872-7573
0378-8741
10.1016/j.jep.2020.113697
2-s2.0-85098865873
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv Journal of Ethnopharmacology
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.source.none.fl_str_mv Scopus
reponame:Repositório Institucional da UNESP
instname:Universidade Estadual Paulista (UNESP)
instacron:UNESP
instname_str Universidade Estadual Paulista (UNESP)
instacron_str UNESP
institution UNESP
reponame_str Repositório Institucional da UNESP
collection Repositório Institucional da UNESP
repository.name.fl_str_mv Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)
repository.mail.fl_str_mv
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