Biotin self-assembled monolayer for impedimetric genosensor for direct detection of HIV-1
Autor(a) principal: | |
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Data de Publicação: | 2020 |
Outros Autores: | , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UNESP |
Texto Completo: | http://dx.doi.org/10.1016/j.microc.2019.104462 http://hdl.handle.net/11449/201371 |
Resumo: | This study was performed to prove the feasibility of a direct impedimetric DNA genosensor by using biotin self-assembled monolayer (SAM). The sensor was composed of a gold rod with a SAM of biotin-modified 19-base oligonucleotide probe (5´-AAA GAT TAA TCC TGG G -3´,) for HIV-1. The electrode modification was characterized by Surface-Enhancement Raman Spectroscopy (SERS), cyclic voltammetry and the hybridization with the complementary oligonucleotide was monitored by impedimetric technique. SERS was used to confirm the linking of Biotin-DNA on the surface of the Au electrode. The coverage was 89% of the surface and a concentration of complementary oligonucleotides of 2.5 10− 12 molL−1 could be detected. |
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Repositório Institucional da UNESP |
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Biotin self-assembled monolayer for impedimetric genosensor for direct detection of HIV-1DNA biosensorsGenosensorHIV-1HybridizationImpedimetricProviral DNAThis study was performed to prove the feasibility of a direct impedimetric DNA genosensor by using biotin self-assembled monolayer (SAM). The sensor was composed of a gold rod with a SAM of biotin-modified 19-base oligonucleotide probe (5´-AAA GAT TAA TCC TGG G -3´,) for HIV-1. The electrode modification was characterized by Surface-Enhancement Raman Spectroscopy (SERS), cyclic voltammetry and the hybridization with the complementary oligonucleotide was monitored by impedimetric technique. SERS was used to confirm the linking of Biotin-DNA on the surface of the Au electrode. The coverage was 89% of the surface and a concentration of complementary oligonucleotides of 2.5 10− 12 molL−1 could be detected.Department of Analytical Chemistry University of HavanaAIDS Research Laboratory (LISIDA)Department of Analytical Chemistry São Paulo State University (UNESP)São Carlos Institute of Chemistry Department of Chemistry and Molecular Physics University of São Paulo (USP)Department of Analytical Chemistry São Paulo State University (UNESP)University of HavanaAIDS Research Laboratory (LISIDA)Universidade Estadual Paulista (Unesp)Universidade de São Paulo (USP)Tamayo, Abel I. BalbinRizo, Laura S. Lópezde Armas, Madelin BlancoFerreira, Antonio Aparecido Pupim [UNESP]Manzani, DaniloYamanaka, Hideko [UNESP]Guas, Ana Margarita Esteva2020-12-12T02:30:48Z2020-12-12T02:30:48Z2020-03-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articlehttp://dx.doi.org/10.1016/j.microc.2019.104462Microchemical Journal, v. 153.0026-265Xhttp://hdl.handle.net/11449/20137110.1016/j.microc.2019.1044622-s2.0-85075892190Scopusreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengMicrochemical Journalinfo:eu-repo/semantics/openAccess2021-10-22T17:50:38Zoai:repositorio.unesp.br:11449/201371Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462024-08-05T17:14:40.585325Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false |
dc.title.none.fl_str_mv |
Biotin self-assembled monolayer for impedimetric genosensor for direct detection of HIV-1 |
title |
Biotin self-assembled monolayer for impedimetric genosensor for direct detection of HIV-1 |
spellingShingle |
Biotin self-assembled monolayer for impedimetric genosensor for direct detection of HIV-1 Tamayo, Abel I. Balbin DNA biosensors Genosensor HIV-1 Hybridization Impedimetric Proviral DNA |
title_short |
Biotin self-assembled monolayer for impedimetric genosensor for direct detection of HIV-1 |
title_full |
Biotin self-assembled monolayer for impedimetric genosensor for direct detection of HIV-1 |
title_fullStr |
Biotin self-assembled monolayer for impedimetric genosensor for direct detection of HIV-1 |
title_full_unstemmed |
Biotin self-assembled monolayer for impedimetric genosensor for direct detection of HIV-1 |
title_sort |
Biotin self-assembled monolayer for impedimetric genosensor for direct detection of HIV-1 |
author |
Tamayo, Abel I. Balbin |
author_facet |
Tamayo, Abel I. Balbin Rizo, Laura S. López de Armas, Madelin Blanco Ferreira, Antonio Aparecido Pupim [UNESP] Manzani, Danilo Yamanaka, Hideko [UNESP] Guas, Ana Margarita Esteva |
author_role |
author |
author2 |
Rizo, Laura S. López de Armas, Madelin Blanco Ferreira, Antonio Aparecido Pupim [UNESP] Manzani, Danilo Yamanaka, Hideko [UNESP] Guas, Ana Margarita Esteva |
author2_role |
author author author author author author |
dc.contributor.none.fl_str_mv |
University of Havana AIDS Research Laboratory (LISIDA) Universidade Estadual Paulista (Unesp) Universidade de São Paulo (USP) |
dc.contributor.author.fl_str_mv |
Tamayo, Abel I. Balbin Rizo, Laura S. López de Armas, Madelin Blanco Ferreira, Antonio Aparecido Pupim [UNESP] Manzani, Danilo Yamanaka, Hideko [UNESP] Guas, Ana Margarita Esteva |
dc.subject.por.fl_str_mv |
DNA biosensors Genosensor HIV-1 Hybridization Impedimetric Proviral DNA |
topic |
DNA biosensors Genosensor HIV-1 Hybridization Impedimetric Proviral DNA |
description |
This study was performed to prove the feasibility of a direct impedimetric DNA genosensor by using biotin self-assembled monolayer (SAM). The sensor was composed of a gold rod with a SAM of biotin-modified 19-base oligonucleotide probe (5´-AAA GAT TAA TCC TGG G -3´,) for HIV-1. The electrode modification was characterized by Surface-Enhancement Raman Spectroscopy (SERS), cyclic voltammetry and the hybridization with the complementary oligonucleotide was monitored by impedimetric technique. SERS was used to confirm the linking of Biotin-DNA on the surface of the Au electrode. The coverage was 89% of the surface and a concentration of complementary oligonucleotides of 2.5 10− 12 molL−1 could be detected. |
publishDate |
2020 |
dc.date.none.fl_str_mv |
2020-12-12T02:30:48Z 2020-12-12T02:30:48Z 2020-03-01 |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://dx.doi.org/10.1016/j.microc.2019.104462 Microchemical Journal, v. 153. 0026-265X http://hdl.handle.net/11449/201371 10.1016/j.microc.2019.104462 2-s2.0-85075892190 |
url |
http://dx.doi.org/10.1016/j.microc.2019.104462 http://hdl.handle.net/11449/201371 |
identifier_str_mv |
Microchemical Journal, v. 153. 0026-265X 10.1016/j.microc.2019.104462 2-s2.0-85075892190 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Microchemical Journal |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.source.none.fl_str_mv |
Scopus reponame:Repositório Institucional da UNESP instname:Universidade Estadual Paulista (UNESP) instacron:UNESP |
instname_str |
Universidade Estadual Paulista (UNESP) |
instacron_str |
UNESP |
institution |
UNESP |
reponame_str |
Repositório Institucional da UNESP |
collection |
Repositório Institucional da UNESP |
repository.name.fl_str_mv |
Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP) |
repository.mail.fl_str_mv |
|
_version_ |
1808128777836822528 |