Distribuição de neurônios imunorreativos para calbindina e calretinina no córtex pré-frontal do macaco-prego - Sapajus (Cebus apella)

• Autor(a) principal: Tessarin, Gestter Willian Lattari [UNESP]
• Data de Publicação: 2013
• Tipo de documento: Trabalho de conclusão de curso
• Idioma: por
• Título da fonte: Repositório Institucional da UNESP
• Texto Completo: http://hdl.handle.net/11449/149606; http://www.athena.biblioteca.unesp.br/exlibris/bd/capelo/2017-01-30/000856436.pdf
• topic: Córtex pré-frontal; Proteínas de ligação ao cálcio; Calbindinas; Calbindina 2; Cebus; Prefrontal cortex

The prefrontal cortex (PfC) occupies the most anterior region of the frontal lobe, showing from a phylogenetic viewpoint a progressive increase of surface. This development has been associated with the acquisition of increasingly sophisticated behaviors. Although the PfC of Old World monkeys (catarrhines) has been extensively studied, the same does not happen with the PfC of platyrrhine monkeys. The evolutionary history of New World monkeys is still open to question, but it is accepted that they evolved independently of Old World monkeys for a period of approximately 30 million years. From an evolutionary perspective, it is important to understand how this process of parallel evolution reflected in the organization of cortical circuitry, and thus the distribution of neuroactive elements can provide relevant information. We analyzed in this study the distribution of neurons that express calbindin and calretinin in six areas of the prefrontal cortex in Capuchin monkey (Sapajus apella) as well as its cellular morphology. Two young adult animals were used originated from the Primate Center at the School of Dentistry of Araçatuba (UNESP - University Estadual Paulista, São Paulo, Brazil). The animals were perfused and decapitated and the region containing the prefrontal cortex was removed according to the pre-determined stereotactic plans. The block was later cryoprotected and sectioned using freezing microtome, producing 40μm width cuts. They were subjected to the immuno-histochemical techniques in order to highlight calbindin and calretinin. Immunoreactive neurons to each technique were quantified using the Image-Pro Plus software and then their neuronal densities were determined (number of neurons per mm2). In order to determine eventual differences between areas the Kruskal-Wallis non-parametric test and the...