Evaluation of hsp65 Nested PCR-Restriction Analysis (PRA) for Diagnosing Tuberculosis in a High Burden Country

• Autor(a) principal: Macente, Sara
• Data de Publicação: 2013
• Outros Autores: Leite, Clarice Queico Fujimura [UNESP], Barreto Santos, Adolfo Carlos [UNESP], Dias Siqueira, Vera Lucia, Cosmo Machado, Luzia Neri, Marcondes, Nadir Rodrigues, Hirata, Mario Hiroyuki, Crespo Hirata, Rosario Dominguez, Cardoso, Rosilene Fressatti
• Tipo de documento: Artigo
• Idioma: eng
• Título da fonte: Repositório Institucional da UNESP
• Texto Completo: http://dx.doi.org/10.1155/2013/391549; http://hdl.handle.net/11449/113446

Current study evaluated the hsp65 Nested PCR Restriction Fragment Length Polymorphism Analysis (hsp65 Nested PCR-PRA) to detect and identify Mycobacterium tuberculosis complex directly in clinical samples for a rapid and specific diagnosis of tuberculosis (TB). hsp65 Nested PCR-PRA was applied directly to 218 clinical samples obtained from 127 patients suspected of TB or another mycobacterial infection from July 2009 to July 2010. The hsp65 Nested PCR-PRA showed 100% sensitivity and 95.0 and 93.1% specificity in comparison with culture and microscopy (acid fast bacillus smear), respectively. hsp65 Nested PCR-PRA was shown to be a fast and reliable assay for diagnosing TB, which may contribute towards a fast diagnosis that could help the selection of appropriate chemotherapeutic and early epidemiological management of the cases which are of paramount importance in a high TB burden country.